Kartikeya Tiwari | Management and science university,Malaysia (original) (raw)

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Papers by Kartikeya Tiwari

Reviews in Medical Microbiology

F1000Research

Background: A large number of undiscovered fungal species still exist on earth, which can be usef... more Background: A large number of undiscovered fungal species still exist on earth, which can be useful for bioprospecting, particularly for single cell oil (SCO) production. Mortierella is one of the significant genera in this field and contains about hundred species. Moreover, M. alpina is the main single cell oil producer at commercial scale under this genus. Methods: Soil samples from four unique locations of North-East Libya were collected for the isolation of oleaginous Mortierella alpina strains by a serial dilution method. Morphological identification was carried out using light microscopy (Olympus, Japan) and genetic diversity of the isolated Mortierella alpina strains was assessed using conserved internal transcribed spacer (ITS) gene sequences available on the NCBI GenBank database for the confirmation of novelty. The nucleotide sequences reported in this study have been deposited at GenBank (accession no. MZ298831:MZ298835). The MultAlin program was used to align the sequenc...

INTERNATIONAL JOURNAL OF MEDICAL TOXICOLOGY & LEGAL MEDICINE

Agricultural Science Digest - A Research Journal

Background: A large number of bioactive compounds are produced by neem tree (Azadirachta indica).... more Background: A large number of bioactive compounds are produced by neem tree (Azadirachta indica). The association between the neem tree and endophytes is not well explored in West Malaysia. Endophytes are the organisms that live inside the medicinal plants and produce bioactive compounds of medicinal importance. The present research work has been carried out to harness significant biocontrol activity showing endophytic bacterial strains. Methods: Screening for bacterial endophytes was performed by using surface sterilisation method. Sterile explants were placed on nutrient agar plates and incubated at 37oC. Plates were observed for the presence of endophytes surrounding the explants. The bacterial endophytes were identified microscopically by Gram staining protocol and molecular characterization was performed by amplification of 16 S rRNA and sequencing. Result: Twelve endophytic bacterial strains isolated in which, only two bacterial strains were found significant in terms of bio-c...

Journal of Biodiversity, Bioprospecting and Development

International Journal of Medical Toxicology & Legal Medicine

Journal of Natural & Ayurvedic Medicine

Asian Journal of Biological Sciences

Journal of Pure and Applied Microbiology

Mycotoxins are a variety of critical secondary metabolites for the defense, that produced by mult... more Mycotoxins are a variety of critical secondary metabolites for the defense, that produced by multiple types of fungi. These metabolites are toxins where metabolic pathways that produce these toxins are found in adjacent gene groups in the fungal genome when they have adequate environmental and dietary conditions. Mainly, they found in commodities stored by the wrong ways. Mycotoxins are the most potent known toxins that cause serious diseases with minimal concentrations. Genetic diversity was detected using polymorphic randomized amplification technique for DNA fragments between fungal isolates from different crops. This review article aims to review the current status of genetically diverse of mycotoxigenic fungi in various contaminated food. Several studies that have focused on the determination of prevalence and frequency of varies types of toxic fungi were reviewed. Also, the articles that study the toxicity of stored crops such as cereals and oilseeds were considered. The high contrast between findings of these works was presented in terms of the genetic diversity of fungal isolates produced toxins. Aspergillus, Fusarium, and Penicillium were observed among the most common fungus producing toxins. This study which derived from previous researches observed that Aflatoxin was the most toxin produced by most fungi. Aspergillus was the most genetically modified fungus, carrying the most genes responsible for producing the fungal toxins.

Journal of Biodiversity, Bioprospecting and Development, 2014

Higher plants are ubiquitously colonized with bacterial endophytes that often lack readily detect... more Higher plants are ubiquitously colonized with bacterial endophytes that often lack readily detectable structures. In the present study seven novel endophytic bacterial species viz; Bacillus amyloliquefaciens (JNU-001), Burkholderia denitrificans (JNU-002), Pseudomonas aeruginosa (JNU-003), Xanthomonas campestris (JNU-004), Azotobacter tropicalis (JNU-005), Acetobacter xylinum (JNU-006) and Azospirillum lipoferum (JNU-007) has been recovered from native neem varieties at Sanganer areas namely Khonagorion, Muhana, Jagatpura, Goner, Watika, Kalwara and Bagru of Rajasthan state. In which Bacillus amyloliquefaciens was found dominant and associated endophytically in all the plants. Molecular characterization of the isolates was performed using 16S r-DNA restriction analysis. Similarity index in unweighted pair group method with arithmetic mean programme clustered the isolates according to their geographical distribution and confirmed the endophytic association between these novel bacterial strains and neem varieties.

Pakistan Journal of Biological Sciences, 2013

SpringerPlus, 2013

Higher plants are ubiquitously colonized with fungal endophytes that often lack readily detectabl... more Higher plants are ubiquitously colonized with fungal endophytes that often lack readily detectable structures. Current study examines the distribution of endophytic fungal communities within Karanja plants and diversity of novel fungal endophyte Alternaria solani isolates collected from different locations of Sanganer region of Rajasthan. Results confirmed that A. solani is a major fungal endophyte consortium associated with Karanja plants. PCR Amplified fragments using random amplified polymorphic DNA (RAPD) primers were subjected to unweighted pair group method analysis (UPGMA), which clearly distinguished twelve ecologically diverse A. solani isolates. A total of 58 RAPD loci were amplified, out of which 35 (60.34%) were polymorphic and 23 were monomorphic (39.66%) in nature. These polymorphic loci were identified with an average of 2.92 bands per primer. The efficacy of RAPD markers proved as an efficient marker system with respect to detection of polymorphism and number of loci scored and can be used for the identification of a particular isolates, thereby defining core collections and strengthening their exploitation in acquiring novel products produced by them.

LS: International Journal of Life Sciences, 2012

As far as microbial world concerned on earth, there are large number of domains has shown the pot... more As far as microbial world concerned on earth, there are large number of domains has shown the potential for various applications. Fungal domain is one of them. Since the discovery of penicillin, various fungi has been harnessed for a large number of applications. Still there is a requirement of novel products for human welfare and development. So what are the fungi of next door? Are the fungal endophytes? Yes? This is the area untouched for a longer period of time. These fungi represent one of the most understudied and diverse group of organisms, are ubiquitous and occur within almost all the plants, including a broad range of hosts in various ecosystems, and therefore play an important role in the natural environment. Despite extensive work in this field, the products of these fungi remains poorly characterized. A large number of endophytic fungi such as Taxomyces, Pestalotiopsis,Muscodor, Piriformospora, Alternaria, Colletotrichum, Cryptosporiopsis, produce enormous valuable products formedicinal, agricultural and industrial importance. Some of the well-known products are taxol (Paclitaxel), serine hydrolases for polyurethane degradation, Annulene (Volatile organic compounds), Colletotric acid and Cryptocandin. So what next, are endophytic fungal metabolites are the future products? The present review explore the potential of these endophytic fungi with respect to the production of various novel secondary metabolites/products in comparison with the traditional fungi of importance.