Bitwell Chibuye | Mukuba University (original) (raw)
Papers by Bitwell Chibuye
Biochemistry and biophysics reports, Sep 1, 2024
Heliyon, Apr 1, 2024
Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they... more Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they lived in their ethnic groupings. To a large extent, it may be true for other ethnic groups and communities across the globe as well. This study aimed to document a drop from the ocean of ethnochemistry knowledge that people in Zambia practiced in the past and modern times to use such ethnochemistry knowledge to teach chemistry in ethnically responsive ways. Further, this study sought to raise the profile of indigenous cultural knowledge in the globalized world dominated by modernity. Ethnography research design was used including unstructured interviews for data collection. This study purposively selected twenty (20) research participants using snowball sampling. Results show that many relevant ethnochemistry practices in Zambia can be used to grow the national economy, enrich the teaching of Secondary School Chemistry to ethnically diverse students, and generate secondary school students' interest and better familiarity with Chemistry.
Scientific African, Jun 1, 2024
Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good heal... more Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good health can be enhanced by including health-promoting foods in the diet without side effects. Adenia panduriformis (Passifloraceae) is a wild vegetable consumed as part of the everyday diet in some communities in Zambia. The plant is also used in traditional medicine to effectively treat and manage many diseases, including degenerative diseases. Because of significant health benefits and medicinal attributes, this vegetable has very high merit to be investigated for its phytochemicals and bioactivities. Therefore, this study screened phytochemicals using qualitative chemical tests and Liquid chromatography-tandem mass spectrometry (LCMS/MS), identified metabolites using molecular networking tools and evaluated the total polyphenolic content and antioxidant activity of the methanolic leaf extract of the vegetable. Solvent extraction by maceration recovered metabolites for screening by qualitative chemical tests, extraction by QuEChERS (quick, easy, cheap, effective, rugged, and safe) was used to recover metabolites for screening by LCMS/MS, and tentative phytochemical constituents were identified using MZmine3, GNPS (global natural products social molecular networking) and METLIN. Total phenolic content (TPC) determination used the Folin-Ciocalteu (FC) method at 765 nm. Total flavonoid content (TFC) was evaluated using the aluminium chloride colorimetric assay at 510 nm. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay at 517 nm. Phytochemical screening by qualitative chemical tests revealed the presence of many classes of phytocompounds, such as alkaloids, polyphenols, terpenoids, and saponins. TPC was determined to be 122.30 ± 2.00 mg GAE/g, and TFC was 60.17 ± 0.25 mg QE/g. Screening by LCMS/MS revealed the presence of many metabolites, of which 23 phytocompounds were identified using MZmine3, 42 by GNPS, and 21 by METLIN. The crude extract exhibited high DPPH free radical quenching activity with a relatively low IC50 value of 53.48 µg/ mL compared to the reference standard ascorbic acid IC50 of 74.47 µg/mL. The significantly lower IC50 value for the wild vegetable than that of ascorbic acid indicates a considerably high efficiency of the metabolites in the vegetable as antioxidants. The presence of many metabolites with health-promoting properties and high antioxidant activity of the leaf extract of A. panduriformis may be the science behind its remarkable health benefits and power to treat and manage a variety of diseases, including degenerative illnesses. This study presents results that indicate the potential of A. panduriformis as a rich source of metabolites with brilliant health-promoting properties and high antioxidant activity, which may open exciting opportunities for further development in the fields of pharmacology and drug development.
Elsevier , 2024
In silico and ADMET molecular analysis targeted to discover novel anti-inflammatory drug candidat... more In silico and ADMET molecular analysis targeted to discover novel anti-inflammatory drug candidates as COX-2 inhibitors from specific metabolites of Diospyros batokana (Ebenaceae)
Heliyon, 2024
Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they... more Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they lived in their ethnic groupings. To a large extent, it may be true for other ethnic groups and communities across the globe as well. This study aimed to document a drop from the ocean of ethnochemistry knowledge that people in Zambia practiced in the past and modern times to use such ethnochemistry knowledge to teach chemistry in ethnically responsive ways. Further, this study sought to raise the profile of indigenous cultural knowledge in the globalized world dominated by modernity. Ethnography research design was used including unstructured interviews for data collection. This study purposively selected twenty (20) research participants using snowball sampling. Results show that many relevant ethnochemistry practices in Zambia can be used to grow the national economy, enrich the teaching of Secondary School Chemistry to ethnically diverse students, and generate secondary school students' interest and better familiarity with Chemistry.
More than 277 distinct forms of cancer disease are referred to as cancer in the broadest sense. R... more More than 277 distinct forms of cancer disease are referred to as cancer in the broadest sense. Researchers have discovered various cancer stages, suggesting that a number of gene alterations have a role in the genesis of cancer. Anomalous cell proliferation results from these gene alterations. Millions of people worldwide are still impacted by the complicated and common health issue that is breast cancer. In this paper, the complex field of breast cancer is thoroughly examined, with a focus on the wide range of risk factors that contribute to the disease's occurrence and the developments in diagnostic techniques. Many risk variables, including genetic predispositions such mutations, hormone effects, lifestyle factors, and reproductive patterns, have been found by a thorough review of the literature published recently. The complex web of elements that contribute to breast cancer etiology also includes age, family history, and environmental factors. New technologies such as genetic testing and magnetic resonance imaging complement mammography, the mainstay of breast cancer screening, to improve the sensitivity and specificity of breast cancer diagnosis. Even with these developments, it is still difficult to guarantee that screening programs are widely accessible, especially in environments with low resources. To sum up, this review highlights the significance of comprehending many risk variables in the emergence of breast cancer and stresses the vital role that developing diagnostic methods have in improving early detection. The goal of this review's synthesis of the available data is to further our understanding of the complex nature of breast cancer and provide guidance for future investigations, screening methods, and preventative measures.
Scientific African, 2024
Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good hea... more Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good
health can be enhanced by including health-promoting foods in the diet without side effects.
Adenia panduriformis (Passifloraceae) is a wild vegetable consumed as part of the everyday diet in
some communities in Zambia. The plant is also used in traditional medicine to effectively treat
and manage many diseases, including degenerative diseases. Because of significant health benefits and medicinal attributes, this vegetable has very high merit to be investigated for its phytochemicals and bioactivities. Therefore, this study screened phytochemicals using qualitative
chemical tests and Liquid chromatography-tandem mass spectrometry (LCMS/MS), identified
metabolites using molecular networking tools and evaluated the total polyphenolic content and
antioxidant activity of the methanolic leaf extract of the vegetable. Solvent extraction by
maceration recovered metabolites for screening by qualitative chemical tests, extraction by
QuEChERS (quick, easy, cheap, effective, rugged, and safe) was used to recover metabolites for
screening by LCMS/MS, and tentative phytochemical constituents were identified using
MZmine3, GNPS (global natural products social molecular networking) and METLIN. Total
phenolic content (TPC) determination used the Folin-Ciocalteu (FC) method at 765 nm. Total
flavonoid content (TFC) was evaluated using the aluminium chloride colorimetric assay at 510
nm. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical
scavenging assay at 517 nm. Phytochemical screening by qualitative chemical tests revealed the
presence of many classes of phytocompounds, such as alkaloids, polyphenols, terpenoids, and
saponins. TPC was determined to be 122.30 ± 2.00 mg GAE/g, and TFC was 60.17 ± 0.25 mg
QE/g. Screening by LCMS/MS revealed the presence of many metabolites, of which 23 phytocompounds were identified using MZmine3, 42 by GNPS, and 21 by METLIN. The crude extract
exhibited high DPPH free radical quenching activity with a relatively low IC50 value of 53.48 µg/
mL compared to the reference standard ascorbic acid IC50 of 74.47 µg/mL. The significantly lower
IC50 value for the wild vegetable than that of ascorbic acid indicates a considerably high efficiency of the metabolites in the vegetable as antioxidants. The presence of many metabolites with
health-promoting properties and high antioxidant activity of the leaf extract of A. panduriformis
may be the science behind its remarkable health benefits and power to treat and manage a variety
of diseases, including degenerative illnesses. This study presents results that indicate the potential of A. panduriformis as a rich source of metabolites with brilliant health-promoting properties and
high antioxidant activity, which may open exciting opportunities for further development in the
fields of pharmacology and drug development.
Social Science Research Network, 2022
Journal of Education and Practice, 2016
The main purpose of the study was to find out the effect of ethnochemistry practices on secondary... more The main purpose of the study was to find out the effect of ethnochemistry practices on secondary school students' attitude towards Chemistry. The design of the study was pre-test post-test control group quasiexperimental design. Two grade 11 intact classes were assigned into experimental and control groups randomly. The total sample size comprised 113 students. To assess attitude of the student towards Chemistry, a chemistry attitude questionnaire was used. An independent samples t-test at an alpha (α) = .05 was conducted to analyze the results of the pre-test and post-test scores. There was a statistically significant difference in the post-test attitude scores for control (M=74.71, SD=13.90) and experimental (M=83.36, S=14.68) groups; t (111) =-3.22, p = 0.002. Therefore, incorporating ethnochemistry practices in teaching chemistry was found to have a positive effect on enhancing secondary school students' attitude towards Chemistry.
Scientific African
These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylo... more These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylocarpon leaves, stem bark, and roots in the treatment of oxidative stress-related disorders such as diabetes mellitus, chronic wounds, and diarrhea. This study may lead to the production of new herbal medicines using D. condylocarpon.
World journal of research and review, Mar 2, 2022
The purpose of the study was to determine the effect of Dialectical Constructivist learning appro... more The purpose of the study was to determine the effect of Dialectical Constructivist learning approach on the attitude and performance of second-year Nutritional Sciences students in Introduction to Biochemistry course. The sample size of the study was 75 students, 38 were in the control group, and 37 were in the experimental group. The research subjects were purposively sampled but randomly assigned to control and experimental groups. Two research instruments were used for data collection. The first was Attitude towards Biochemistry Questionnaire and the second was a Biochemistry Performance Test. The design of the study was pre-test post-test control group quasi experimental design. The students in the experimental group were taught Biochemistry course using Dialectical constructivist learning approach, whereas students in the control group were taught using the conventional approach. To assess attitude of the students towards Biochemistry, a Biochemistry Attitude Questionnaire was used. There was enhancement of attitude towards Biochemistry in the experimental group than in the control group owing to a significant change of attitude Mean. Therefore, using Dialectical constructivism in teaching Biochemistry was found to have a positive effect on enhancing Nutritional Science students' attitude towards Biochemistry. Further, to assess the achievement of students towards Biochemistry, a Biochemistry achievement test was used. An independent samples t-test at an alpha (α) = .05 was conducted to analyze the results of the pre-test and post-test scores. There was a significant difference in the scores for experimental (M=58.9189, SD=15.01551) and control (M=43.4474, SD=13.92392) groups; t (73) =4.629, p = 0.000 The magnitude of the differences in the means was large (eta squared =.227). Therefore, using Dialectical Constructivism in teaching Introduction to Biochemistry course was found to have a positive effect on enhancing students' performance in the course.
For centuries, phytochemicals have been of immense value to communities worldwide. These metaboli... more For centuries, phytochemicals have been of immense value to communities worldwide. These metabolites have been used in healthcare systems as medicines to treat various diseases. Further, phytochemicals are used as lead compounds in the synthesis of drugs. The extraction of compounds from plant materials is the cornerstone of natural product research. There has been a relentless endeavor to discover better extractive methods. In the same vein, several promising modern green extraction methods such as supercritical fluid, ultrasound, accelerated solvent, microwave, enzyme-assisted extraction methods are gaining significance. This review describes and discusses the various extraction techniques used to obtain the phytochemicals from different plant parts. These extraction techniques include the conventional solvent-based and the more robust modern and green extraction techniques. The review critically analyses the extraction conditions, optimized situations, advantages, and disadvantages of these extraction techniques. The review includes the recent applications of these extraction techniques. The review will propel advanced research and applications in the extraction process, a significant and integral component of natural products research.
These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylo... more These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylocarpon leaves, stem bark, and roots in the treatment of oxidative stress-related disorders such as diabetes mellitus, chronic wounds, and diarrhea. This study may lead to the production of new herbal medicines using D. condylocarpon.
Scientific African, 2023
These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylo... more These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylocarpon leaves, stem bark, and roots in the treatment of oxidative stress-related disorders such as diabetes mellitus, chronic wounds, and diarrhea. This study may lead to the production of new herbal medicines using D. condylocarpon.
Scientific African, 2023
For centuries, phytochemicals have been of immense value to communities worldwide. These metaboli... more For centuries, phytochemicals have been of immense value to communities worldwide. These metabolites have been used in healthcare systems as medicines to treat various diseases. Further, phytochemicals are used as lead compounds in the synthesis of drugs. The extraction of compounds from plant materials is the cornerstone of natural product research. There has been a relentless endeavor to discover better extractive methods. In the same vein, several promising modern green extraction methods such as supercritical fluid, ultrasound, accelerated solvent, microwave, enzyme-assisted extraction methods are gaining significance. This review describes and discusses the various extraction techniques used to obtain the phytochemicals from different plant parts. These extraction techniques include the conventional solvent-based and the more robust modern and green extraction techniques. The review critically analyses the extraction conditions, optimized situations, advantages, and disadvantages of these extraction techniques. The review includes the recent applications of these extraction techniques. The review will propel advanced research and applications in the extraction process, a significant and integral component of natural products research.
Scientific African
For centuries, phytochemicals have been of immense value to communities worldwide. These metaboli... more For centuries, phytochemicals have been of immense value to communities worldwide. These metabolites have been used in healthcare systems as medicines to treat various diseases. Further, phytochemicals are used as lead compounds in the synthesis of drugs. The extraction of compounds from plant materials is the cornerstone of natural product research. There has been a relentless endeavor to discover better extractive methods. In the same vein, several promising modern green extraction methods such as supercritical fluid, ultrasound, accelerated solvent, microwave, enzyme-assisted extraction methods are gaining significance. This review describes and discusses the various extraction techniques used to obtain the phytochemicals from different plant parts. These extraction techniques include the conventional solvent-based and the more robust modern and green extraction techniques. The review critically analyses the extraction conditions, optimized situations, advantages, and disadvantages of these extraction techniques. The review includes the recent applications of these extraction techniques. The review will propel advanced research and applications in the extraction process, a significant and integral component of natural products research.
Journal of Education and Practice, 2019
The study was undertaken to establish the effect of Algebraic Method on Secondary School Students... more The study was undertaken to establish the effect of Algebraic Method on Secondary School Students' performance in balancing chemical equations. The sample of the study consisted of 81 Grade 11 students from two intact classes which were purposively selected but randomly assigned to experimental and control groups. The design of the study was pre-test post-test control group quasi-experimental design. The study subjects in the experimental group were taught using Algebraic method, while the control group was taught using the conventional Inspection method over a period of four weeks. Balancing Chemical Equation Performance Test (BCEPT) was used to determine the performance of the two groups after treatment. An independent samples t-test at an alpha level (α) = .05 was conducted to compare the performance of the groups during both the pre-test and post-test. The results of pre-test revealed that there was no significant difference in the performance of both groups. This result implied that the experimental and control groups were equivalent in terms of performance before treatment was administered to the experimental group. However, during post-test there was a statistically significant difference in the mean performance scores of experimental (M = 62.2, SD = 21.8) group and control (M =49.7, SD = 18.0) group t (79) = 2.817, p = .006. The implicature was that students taught balancing chemical equations using Algebraic method performed better than those taught using Inspection method.
Journal of Education and Practice, 2016
The main purpose of the study was to find out the effect of ethnochemistry practices on secondary... more The main purpose of the study was to find out the effect of ethnochemistry practices on secondary school students’ attitude towards Chemistry. The design of the study was pre-test post-test control group quasi-experimental design. Two grade 11 intact classes were assigned into experimental and control groups randomly. The total sample size comprised 113 students. To assess attitude of the student towards Chemistry, a chemistry attitude questionnaire was used. An independent samples t-test at an alpha (α) = .05 was conducted to analyze the results of the pre-test and post-test scores. There was a statistically significant difference in the post-test attitude scores for control (M=74.71, SD=13.90) and experimental (M=83.36, S=14.68) groups; t (111) = -3.22, p = 0.002. Therefore, incorporating ethnochemistry practices in teaching chemistry was found to have a positive effect on enhancing secondary school students’ attitude towards Chemistry. Keywords: Ethnochemistry, Conventional app...
Biochemistry and biophysics reports, Sep 1, 2024
Heliyon, Apr 1, 2024
Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they... more Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they lived in their ethnic groupings. To a large extent, it may be true for other ethnic groups and communities across the globe as well. This study aimed to document a drop from the ocean of ethnochemistry knowledge that people in Zambia practiced in the past and modern times to use such ethnochemistry knowledge to teach chemistry in ethnically responsive ways. Further, this study sought to raise the profile of indigenous cultural knowledge in the globalized world dominated by modernity. Ethnography research design was used including unstructured interviews for data collection. This study purposively selected twenty (20) research participants using snowball sampling. Results show that many relevant ethnochemistry practices in Zambia can be used to grow the national economy, enrich the teaching of Secondary School Chemistry to ethnically diverse students, and generate secondary school students' interest and better familiarity with Chemistry.
Scientific African, Jun 1, 2024
Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good heal... more Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good health can be enhanced by including health-promoting foods in the diet without side effects. Adenia panduriformis (Passifloraceae) is a wild vegetable consumed as part of the everyday diet in some communities in Zambia. The plant is also used in traditional medicine to effectively treat and manage many diseases, including degenerative diseases. Because of significant health benefits and medicinal attributes, this vegetable has very high merit to be investigated for its phytochemicals and bioactivities. Therefore, this study screened phytochemicals using qualitative chemical tests and Liquid chromatography-tandem mass spectrometry (LCMS/MS), identified metabolites using molecular networking tools and evaluated the total polyphenolic content and antioxidant activity of the methanolic leaf extract of the vegetable. Solvent extraction by maceration recovered metabolites for screening by qualitative chemical tests, extraction by QuEChERS (quick, easy, cheap, effective, rugged, and safe) was used to recover metabolites for screening by LCMS/MS, and tentative phytochemical constituents were identified using MZmine3, GNPS (global natural products social molecular networking) and METLIN. Total phenolic content (TPC) determination used the Folin-Ciocalteu (FC) method at 765 nm. Total flavonoid content (TFC) was evaluated using the aluminium chloride colorimetric assay at 510 nm. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay at 517 nm. Phytochemical screening by qualitative chemical tests revealed the presence of many classes of phytocompounds, such as alkaloids, polyphenols, terpenoids, and saponins. TPC was determined to be 122.30 ± 2.00 mg GAE/g, and TFC was 60.17 ± 0.25 mg QE/g. Screening by LCMS/MS revealed the presence of many metabolites, of which 23 phytocompounds were identified using MZmine3, 42 by GNPS, and 21 by METLIN. The crude extract exhibited high DPPH free radical quenching activity with a relatively low IC50 value of 53.48 µg/ mL compared to the reference standard ascorbic acid IC50 of 74.47 µg/mL. The significantly lower IC50 value for the wild vegetable than that of ascorbic acid indicates a considerably high efficiency of the metabolites in the vegetable as antioxidants. The presence of many metabolites with health-promoting properties and high antioxidant activity of the leaf extract of A. panduriformis may be the science behind its remarkable health benefits and power to treat and manage a variety of diseases, including degenerative illnesses. This study presents results that indicate the potential of A. panduriformis as a rich source of metabolites with brilliant health-promoting properties and high antioxidant activity, which may open exciting opportunities for further development in the fields of pharmacology and drug development.
Elsevier , 2024
In silico and ADMET molecular analysis targeted to discover novel anti-inflammatory drug candidat... more In silico and ADMET molecular analysis targeted to discover novel anti-inflammatory drug candidates as COX-2 inhibitors from specific metabolites of Diospyros batokana (Ebenaceae)
Heliyon, 2024
Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they... more Before formally introducing chemistry in schools, Africans practiced it as ethnochemistry as they lived in their ethnic groupings. To a large extent, it may be true for other ethnic groups and communities across the globe as well. This study aimed to document a drop from the ocean of ethnochemistry knowledge that people in Zambia practiced in the past and modern times to use such ethnochemistry knowledge to teach chemistry in ethnically responsive ways. Further, this study sought to raise the profile of indigenous cultural knowledge in the globalized world dominated by modernity. Ethnography research design was used including unstructured interviews for data collection. This study purposively selected twenty (20) research participants using snowball sampling. Results show that many relevant ethnochemistry practices in Zambia can be used to grow the national economy, enrich the teaching of Secondary School Chemistry to ethnically diverse students, and generate secondary school students' interest and better familiarity with Chemistry.
More than 277 distinct forms of cancer disease are referred to as cancer in the broadest sense. R... more More than 277 distinct forms of cancer disease are referred to as cancer in the broadest sense. Researchers have discovered various cancer stages, suggesting that a number of gene alterations have a role in the genesis of cancer. Anomalous cell proliferation results from these gene alterations. Millions of people worldwide are still impacted by the complicated and common health issue that is breast cancer. In this paper, the complex field of breast cancer is thoroughly examined, with a focus on the wide range of risk factors that contribute to the disease's occurrence and the developments in diagnostic techniques. Many risk variables, including genetic predispositions such mutations, hormone effects, lifestyle factors, and reproductive patterns, have been found by a thorough review of the literature published recently. The complex web of elements that contribute to breast cancer etiology also includes age, family history, and environmental factors. New technologies such as genetic testing and magnetic resonance imaging complement mammography, the mainstay of breast cancer screening, to improve the sensitivity and specificity of breast cancer diagnosis. Even with these developments, it is still difficult to guarantee that screening programs are widely accessible, especially in environments with low resources. To sum up, this review highlights the significance of comprehending many risk variables in the emergence of breast cancer and stresses the vital role that developing diagnostic methods have in improving early detection. The goal of this review's synthesis of the available data is to further our understanding of the complex nature of breast cancer and provide guidance for future investigations, screening methods, and preventative measures.
Scientific African, 2024
Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good hea... more Diseases, especially degenerative illnesses, can be treated and avoided altogether, and good
health can be enhanced by including health-promoting foods in the diet without side effects.
Adenia panduriformis (Passifloraceae) is a wild vegetable consumed as part of the everyday diet in
some communities in Zambia. The plant is also used in traditional medicine to effectively treat
and manage many diseases, including degenerative diseases. Because of significant health benefits and medicinal attributes, this vegetable has very high merit to be investigated for its phytochemicals and bioactivities. Therefore, this study screened phytochemicals using qualitative
chemical tests and Liquid chromatography-tandem mass spectrometry (LCMS/MS), identified
metabolites using molecular networking tools and evaluated the total polyphenolic content and
antioxidant activity of the methanolic leaf extract of the vegetable. Solvent extraction by
maceration recovered metabolites for screening by qualitative chemical tests, extraction by
QuEChERS (quick, easy, cheap, effective, rugged, and safe) was used to recover metabolites for
screening by LCMS/MS, and tentative phytochemical constituents were identified using
MZmine3, GNPS (global natural products social molecular networking) and METLIN. Total
phenolic content (TPC) determination used the Folin-Ciocalteu (FC) method at 765 nm. Total
flavonoid content (TFC) was evaluated using the aluminium chloride colorimetric assay at 510
nm. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical
scavenging assay at 517 nm. Phytochemical screening by qualitative chemical tests revealed the
presence of many classes of phytocompounds, such as alkaloids, polyphenols, terpenoids, and
saponins. TPC was determined to be 122.30 ± 2.00 mg GAE/g, and TFC was 60.17 ± 0.25 mg
QE/g. Screening by LCMS/MS revealed the presence of many metabolites, of which 23 phytocompounds were identified using MZmine3, 42 by GNPS, and 21 by METLIN. The crude extract
exhibited high DPPH free radical quenching activity with a relatively low IC50 value of 53.48 µg/
mL compared to the reference standard ascorbic acid IC50 of 74.47 µg/mL. The significantly lower
IC50 value for the wild vegetable than that of ascorbic acid indicates a considerably high efficiency of the metabolites in the vegetable as antioxidants. The presence of many metabolites with
health-promoting properties and high antioxidant activity of the leaf extract of A. panduriformis
may be the science behind its remarkable health benefits and power to treat and manage a variety
of diseases, including degenerative illnesses. This study presents results that indicate the potential of A. panduriformis as a rich source of metabolites with brilliant health-promoting properties and
high antioxidant activity, which may open exciting opportunities for further development in the
fields of pharmacology and drug development.
Social Science Research Network, 2022
Journal of Education and Practice, 2016
The main purpose of the study was to find out the effect of ethnochemistry practices on secondary... more The main purpose of the study was to find out the effect of ethnochemistry practices on secondary school students' attitude towards Chemistry. The design of the study was pre-test post-test control group quasiexperimental design. Two grade 11 intact classes were assigned into experimental and control groups randomly. The total sample size comprised 113 students. To assess attitude of the student towards Chemistry, a chemistry attitude questionnaire was used. An independent samples t-test at an alpha (α) = .05 was conducted to analyze the results of the pre-test and post-test scores. There was a statistically significant difference in the post-test attitude scores for control (M=74.71, SD=13.90) and experimental (M=83.36, S=14.68) groups; t (111) =-3.22, p = 0.002. Therefore, incorporating ethnochemistry practices in teaching chemistry was found to have a positive effect on enhancing secondary school students' attitude towards Chemistry.
Scientific African
These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylo... more These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylocarpon leaves, stem bark, and roots in the treatment of oxidative stress-related disorders such as diabetes mellitus, chronic wounds, and diarrhea. This study may lead to the production of new herbal medicines using D. condylocarpon.
World journal of research and review, Mar 2, 2022
The purpose of the study was to determine the effect of Dialectical Constructivist learning appro... more The purpose of the study was to determine the effect of Dialectical Constructivist learning approach on the attitude and performance of second-year Nutritional Sciences students in Introduction to Biochemistry course. The sample size of the study was 75 students, 38 were in the control group, and 37 were in the experimental group. The research subjects were purposively sampled but randomly assigned to control and experimental groups. Two research instruments were used for data collection. The first was Attitude towards Biochemistry Questionnaire and the second was a Biochemistry Performance Test. The design of the study was pre-test post-test control group quasi experimental design. The students in the experimental group were taught Biochemistry course using Dialectical constructivist learning approach, whereas students in the control group were taught using the conventional approach. To assess attitude of the students towards Biochemistry, a Biochemistry Attitude Questionnaire was used. There was enhancement of attitude towards Biochemistry in the experimental group than in the control group owing to a significant change of attitude Mean. Therefore, using Dialectical constructivism in teaching Biochemistry was found to have a positive effect on enhancing Nutritional Science students' attitude towards Biochemistry. Further, to assess the achievement of students towards Biochemistry, a Biochemistry achievement test was used. An independent samples t-test at an alpha (α) = .05 was conducted to analyze the results of the pre-test and post-test scores. There was a significant difference in the scores for experimental (M=58.9189, SD=15.01551) and control (M=43.4474, SD=13.92392) groups; t (73) =4.629, p = 0.000 The magnitude of the differences in the means was large (eta squared =.227). Therefore, using Dialectical Constructivism in teaching Introduction to Biochemistry course was found to have a positive effect on enhancing students' performance in the course.
For centuries, phytochemicals have been of immense value to communities worldwide. These metaboli... more For centuries, phytochemicals have been of immense value to communities worldwide. These metabolites have been used in healthcare systems as medicines to treat various diseases. Further, phytochemicals are used as lead compounds in the synthesis of drugs. The extraction of compounds from plant materials is the cornerstone of natural product research. There has been a relentless endeavor to discover better extractive methods. In the same vein, several promising modern green extraction methods such as supercritical fluid, ultrasound, accelerated solvent, microwave, enzyme-assisted extraction methods are gaining significance. This review describes and discusses the various extraction techniques used to obtain the phytochemicals from different plant parts. These extraction techniques include the conventional solvent-based and the more robust modern and green extraction techniques. The review critically analyses the extraction conditions, optimized situations, advantages, and disadvantages of these extraction techniques. The review includes the recent applications of these extraction techniques. The review will propel advanced research and applications in the extraction process, a significant and integral component of natural products research.
These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylo... more These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylocarpon leaves, stem bark, and roots in the treatment of oxidative stress-related disorders such as diabetes mellitus, chronic wounds, and diarrhea. This study may lead to the production of new herbal medicines using D. condylocarpon.
Scientific African, 2023
These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylo... more These antioxidant activities support the traditionally claimed therapeutic benefits of D. condylocarpon leaves, stem bark, and roots in the treatment of oxidative stress-related disorders such as diabetes mellitus, chronic wounds, and diarrhea. This study may lead to the production of new herbal medicines using D. condylocarpon.
Scientific African, 2023
For centuries, phytochemicals have been of immense value to communities worldwide. These metaboli... more For centuries, phytochemicals have been of immense value to communities worldwide. These metabolites have been used in healthcare systems as medicines to treat various diseases. Further, phytochemicals are used as lead compounds in the synthesis of drugs. The extraction of compounds from plant materials is the cornerstone of natural product research. There has been a relentless endeavor to discover better extractive methods. In the same vein, several promising modern green extraction methods such as supercritical fluid, ultrasound, accelerated solvent, microwave, enzyme-assisted extraction methods are gaining significance. This review describes and discusses the various extraction techniques used to obtain the phytochemicals from different plant parts. These extraction techniques include the conventional solvent-based and the more robust modern and green extraction techniques. The review critically analyses the extraction conditions, optimized situations, advantages, and disadvantages of these extraction techniques. The review includes the recent applications of these extraction techniques. The review will propel advanced research and applications in the extraction process, a significant and integral component of natural products research.
Scientific African
For centuries, phytochemicals have been of immense value to communities worldwide. These metaboli... more For centuries, phytochemicals have been of immense value to communities worldwide. These metabolites have been used in healthcare systems as medicines to treat various diseases. Further, phytochemicals are used as lead compounds in the synthesis of drugs. The extraction of compounds from plant materials is the cornerstone of natural product research. There has been a relentless endeavor to discover better extractive methods. In the same vein, several promising modern green extraction methods such as supercritical fluid, ultrasound, accelerated solvent, microwave, enzyme-assisted extraction methods are gaining significance. This review describes and discusses the various extraction techniques used to obtain the phytochemicals from different plant parts. These extraction techniques include the conventional solvent-based and the more robust modern and green extraction techniques. The review critically analyses the extraction conditions, optimized situations, advantages, and disadvantages of these extraction techniques. The review includes the recent applications of these extraction techniques. The review will propel advanced research and applications in the extraction process, a significant and integral component of natural products research.
Journal of Education and Practice, 2019
The study was undertaken to establish the effect of Algebraic Method on Secondary School Students... more The study was undertaken to establish the effect of Algebraic Method on Secondary School Students' performance in balancing chemical equations. The sample of the study consisted of 81 Grade 11 students from two intact classes which were purposively selected but randomly assigned to experimental and control groups. The design of the study was pre-test post-test control group quasi-experimental design. The study subjects in the experimental group were taught using Algebraic method, while the control group was taught using the conventional Inspection method over a period of four weeks. Balancing Chemical Equation Performance Test (BCEPT) was used to determine the performance of the two groups after treatment. An independent samples t-test at an alpha level (α) = .05 was conducted to compare the performance of the groups during both the pre-test and post-test. The results of pre-test revealed that there was no significant difference in the performance of both groups. This result implied that the experimental and control groups were equivalent in terms of performance before treatment was administered to the experimental group. However, during post-test there was a statistically significant difference in the mean performance scores of experimental (M = 62.2, SD = 21.8) group and control (M =49.7, SD = 18.0) group t (79) = 2.817, p = .006. The implicature was that students taught balancing chemical equations using Algebraic method performed better than those taught using Inspection method.
Journal of Education and Practice, 2016
The main purpose of the study was to find out the effect of ethnochemistry practices on secondary... more The main purpose of the study was to find out the effect of ethnochemistry practices on secondary school students’ attitude towards Chemistry. The design of the study was pre-test post-test control group quasi-experimental design. Two grade 11 intact classes were assigned into experimental and control groups randomly. The total sample size comprised 113 students. To assess attitude of the student towards Chemistry, a chemistry attitude questionnaire was used. An independent samples t-test at an alpha (α) = .05 was conducted to analyze the results of the pre-test and post-test scores. There was a statistically significant difference in the post-test attitude scores for control (M=74.71, SD=13.90) and experimental (M=83.36, S=14.68) groups; t (111) = -3.22, p = 0.002. Therefore, incorporating ethnochemistry practices in teaching chemistry was found to have a positive effect on enhancing secondary school students’ attitude towards Chemistry. Keywords: Ethnochemistry, Conventional app...