Ali Moradi | Mashhad University of Medical Sciences, Mashhad. Iran. (original) (raw)
Papers by Ali Moradi
Journal of Biomedical Materials Research Part A, 2015
Extracellular matrices have drawn attention in tissue engineering as potential biomaterials for s... more Extracellular matrices have drawn attention in tissue engineering as potential biomaterials for scaffold fabrication because of their bioactive components. Noninvasive techniques of scaffold fabrication and cross-linking treatments are believed to maintain the integrity of bioactive molecules while providing proper architectural and mechanical properties. Cartilage matrix derived scaffolds are designed to support the maintenance of chondrocytes and provide proper signals for differentiation of chondroinducible cells. Chondroinductive potential of bovine articular cartilage matrix derived porous scaffolds on human dermal fibroblasts and the effect of scaffold shrinkage on chondrogenesis were investigated. An increase in sulfated glycosaminoglycans production along with upregulation of chondrogenic genes confirmed that physically treated cartilage matrix derived scaffolds have chondrogenic potential on human dermal fibroblasts. V
Journal of Nanoscience and Nanotechnology, 2011
A novel biodegradable polymer elastomer nanocomposite composing of poly(1,8-octanediol-citrate) (... more A novel biodegradable polymer elastomer nanocomposite composing of poly(1,8-octanediol-citrate) (POC) polymer matrix and carbon nanotubes (CNTs) additive was successfully fabricated and systematically investigated using Fourier transform infrared (FT-IR), X-ray diffractometer (XRD), differential scanning calorimetry (DSC), tensile test, incubation and cytotoxicity tests. It was found that the addition of CNTs in POC elastomer did not result in any noticeable change in its chemical structure and the amorphous state. However, the tensile strength and elongation at break were greatly improved by the addition of CNTs in POC polymer matrix. It revealed that the swelling ratio and percentage of weight loss of POC/CNTs nanocomposite were lower, compared with the pure POC material. Moreover, the adsorption amount of bovine serum albumin (BSA) increased with an increase of the CNTs mass content in POC matrix revealing the enhanced hydrophilicity of POC/CNTs nanocomposites contributed by the carboxyl of the CNTs. Additionally, the cytotoxicity tests with L929 cell line revealed that the experimental POC/CNTs nanocomposites possessed good in vitro biocompatibility.
Journal of Biomedical Materials Research Part A, 2014
Extracellular environments can regulate cell behavior because cells can actively sense their mech... more Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al 2 O 3 ) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt % Al 2 O 3 at a curing temperature of 50 C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1 0 -dioctadecyl-1,3,3,3 0 ,3 0 -tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt % Al 2 O 3 ) exhibited higher stiff-ness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al 2 O 3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells. V C 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 00A: 000-000, 2014.
Abstract: Extracellular matrices have drawn attention in tissue engineering as potential biomater... more Abstract: Extracellular matrices have drawn attention in tissue
engineering as potential biomaterials for scaffold fabrication
because of their bioactive components. Noninvasive techniques
of scaffold fabrication and cross-linking treatments are
believed to maintain the integrity of bioactive molecules while
providing proper architectural and mechanical properties. Cartilage
matrix derived scaffolds are designed to support the
maintenance of chondrocytes and provide proper signals for
differentiation of chondroinducible cells. Chondroinductive
potential of bovine articular cartilage matrix derived porous
scaffolds on human dermal fibroblasts and the effect of scaffold shrinkage on chondrogenesis were investigated. An
increase in sulfated glycosaminoglycans production along
with upregulation of chondrogenic genes confirmed that
physically treated cartilage matrix derived scaffolds have chondrogenic potential on human dermal fibroblasts.
Precise and accurate porosity measurement is essential for characterization of porous materials. ... more Precise and accurate porosity measurement is essential for characterization of porous materials.
Considering Archimedes' principle based liquid displacement methods of measuring porosity we have
developed an excellent modified micro-volumetric method of porosity measurement. Changes in the
liquid level in a glass pipette after immersing and also removing the porous sample were recorded by
using a digital camera and analysed by ImageJ® software. Results of porosity measurement through a
micro-volumetric method were compared with micro-CT results. Bland–Altman analysis showed a
much higher precision and accuracy for our micro-volumetric method (bias ¼0.023, CI: ]0.459,
0.413[, SD ¼ 1.96 Ơ) compared to the micro-CT method (bias ¼ 6.075, CI: ]20.993, 33.142[, SD ¼
1.96 Ơ). Our highly precise and accurate micro-volumetric method of porosity measurement is
particularly applicable to small ultra-light highly porous materials.
Background aims. Numerous protocols for the isolation of bovine aortic endothelial cells have bee... more Background aims. Numerous protocols for the isolation of bovine aortic endothelial cells have been described in the previous literature. However, these protocols prevent researchers from obtaining the pure population of endothelial cells. Thus, this study aimed to develop a new and economical method for the isolation of pure endothelial cells by introducing a new strategy to the enzymatic digestion method proposed by previous researchers. Methods. With the use of this method, the lumen of a bovine aorta was filled with wash medium and the outer surface of the sample was washed with alcohol for 30 seconds. Under a laminar flow hood, the inner surface of the sample was covered with filter paper. Collagenase type II was dripped onto the filter paper as a digestion enzyme. The digestion fluid was seeded in T25 flasks and fed with complete medium every 3 days. Results. The isolated cells were characterized by markers such as CD31, von Willebrand factor, 1,1 0 -dioctadecyl-1,3,3,3 0 ,3 0tetramethylindocarbocyanine perchlorate acetylated low-density lipoprotein and angiogenesis behavior. The purity of endothelial cells was detected by flow cytometry to be of nearly 90% purity; these results were confirmed by immunostaining. Moreover, endothelial cells formed blood vesselelike tubes in a three-dimensional environment, which is specific dynamic behavior for endothelial cells. Conclusions. The new strategy presented in the current report enables isolation of a highly pure population of endothelial cells that can survive long-term culture without inducing an overgrowth of fibroblast cells.
Native cartilage matrix derived (CMD) scaffolds from various animal and human sources have drawn ... more Native cartilage matrix derived (CMD) scaffolds from various animal and human sources have
drawn attention in cartilage tissue engineering due to the demonstrable presence of bioactive
components. Different chemical and physical treatments have been employed to enhance the
micro-architecture of CMD scaffolds. In this study we have assessed the typical effects of
physical cross-linking methods, namely ultraviolet (UV) light, dehydrothermal (DHT) treatment,
and combinations of them on bovine articular CMD porous scaffolds with three different matrix
concentrations (5%, 15% and 30%) to assess the relative strengths of each treatment. Our
findings suggest that UV and UV–DHT treatments on 15% CMD scaffolds can yield
architecturally optimal scaffolds for cartilage tissue engineering.
In this paper we describe an effective technical route for the production of porous scaffolds fro... more In this paper we describe an effective technical route for the production of porous scaffolds from microcomposite material consistent of citric acid-based polyester elastomer and hydroxyapatite microparticles with potential use in tissue engineering applications. Fabricated scaffolds were characterized by field emission electron microscopy, Fourier-transform infra-red spectroscopy, thermal gravimetric analysis and mechanical tests. We demonstrate that the chemical composition, scaffold morphology, mechanical integrity and porosity of the produced microcomposite scaffolds are strongly affected by variation of the initial hydroxyapatite concentration in the mineral-polymer mixture during the fabrication process. This polyester/mineral scaffold exhibits versatility in compression modulus and strength in the range of 21.72-33.25 KPa and 49.92-101.48 KPa respectively, corresponding to the hydroxyapatite concentration in the composite (10-30 wt.%).
Journal of Biomedical Materials Research Part A, 2015
Extracellular matrices have drawn attention in tissue engineering as potential biomaterials for s... more Extracellular matrices have drawn attention in tissue engineering as potential biomaterials for scaffold fabrication because of their bioactive components. Noninvasive techniques of scaffold fabrication and cross-linking treatments are believed to maintain the integrity of bioactive molecules while providing proper architectural and mechanical properties. Cartilage matrix derived scaffolds are designed to support the maintenance of chondrocytes and provide proper signals for differentiation of chondroinducible cells. Chondroinductive potential of bovine articular cartilage matrix derived porous scaffolds on human dermal fibroblasts and the effect of scaffold shrinkage on chondrogenesis were investigated. An increase in sulfated glycosaminoglycans production along with upregulation of chondrogenic genes confirmed that physically treated cartilage matrix derived scaffolds have chondrogenic potential on human dermal fibroblasts. V
Journal of Nanoscience and Nanotechnology, 2011
A novel biodegradable polymer elastomer nanocomposite composing of poly(1,8-octanediol-citrate) (... more A novel biodegradable polymer elastomer nanocomposite composing of poly(1,8-octanediol-citrate) (POC) polymer matrix and carbon nanotubes (CNTs) additive was successfully fabricated and systematically investigated using Fourier transform infrared (FT-IR), X-ray diffractometer (XRD), differential scanning calorimetry (DSC), tensile test, incubation and cytotoxicity tests. It was found that the addition of CNTs in POC elastomer did not result in any noticeable change in its chemical structure and the amorphous state. However, the tensile strength and elongation at break were greatly improved by the addition of CNTs in POC polymer matrix. It revealed that the swelling ratio and percentage of weight loss of POC/CNTs nanocomposite were lower, compared with the pure POC material. Moreover, the adsorption amount of bovine serum albumin (BSA) increased with an increase of the CNTs mass content in POC matrix revealing the enhanced hydrophilicity of POC/CNTs nanocomposites contributed by the carboxyl of the CNTs. Additionally, the cytotoxicity tests with L929 cell line revealed that the experimental POC/CNTs nanocomposites possessed good in vitro biocompatibility.
Journal of Biomedical Materials Research Part A, 2014
Extracellular environments can regulate cell behavior because cells can actively sense their mech... more Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al 2 O 3 ) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt % Al 2 O 3 at a curing temperature of 50 C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1 0 -dioctadecyl-1,3,3,3 0 ,3 0 -tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt % Al 2 O 3 ) exhibited higher stiff-ness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al 2 O 3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells. V C 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 00A: 000-000, 2014.
Abstract: Extracellular matrices have drawn attention in tissue engineering as potential biomater... more Abstract: Extracellular matrices have drawn attention in tissue
engineering as potential biomaterials for scaffold fabrication
because of their bioactive components. Noninvasive techniques
of scaffold fabrication and cross-linking treatments are
believed to maintain the integrity of bioactive molecules while
providing proper architectural and mechanical properties. Cartilage
matrix derived scaffolds are designed to support the
maintenance of chondrocytes and provide proper signals for
differentiation of chondroinducible cells. Chondroinductive
potential of bovine articular cartilage matrix derived porous
scaffolds on human dermal fibroblasts and the effect of scaffold shrinkage on chondrogenesis were investigated. An
increase in sulfated glycosaminoglycans production along
with upregulation of chondrogenic genes confirmed that
physically treated cartilage matrix derived scaffolds have chondrogenic potential on human dermal fibroblasts.
Precise and accurate porosity measurement is essential for characterization of porous materials. ... more Precise and accurate porosity measurement is essential for characterization of porous materials.
Considering Archimedes' principle based liquid displacement methods of measuring porosity we have
developed an excellent modified micro-volumetric method of porosity measurement. Changes in the
liquid level in a glass pipette after immersing and also removing the porous sample were recorded by
using a digital camera and analysed by ImageJ® software. Results of porosity measurement through a
micro-volumetric method were compared with micro-CT results. Bland–Altman analysis showed a
much higher precision and accuracy for our micro-volumetric method (bias ¼0.023, CI: ]0.459,
0.413[, SD ¼ 1.96 Ơ) compared to the micro-CT method (bias ¼ 6.075, CI: ]20.993, 33.142[, SD ¼
1.96 Ơ). Our highly precise and accurate micro-volumetric method of porosity measurement is
particularly applicable to small ultra-light highly porous materials.
Background aims. Numerous protocols for the isolation of bovine aortic endothelial cells have bee... more Background aims. Numerous protocols for the isolation of bovine aortic endothelial cells have been described in the previous literature. However, these protocols prevent researchers from obtaining the pure population of endothelial cells. Thus, this study aimed to develop a new and economical method for the isolation of pure endothelial cells by introducing a new strategy to the enzymatic digestion method proposed by previous researchers. Methods. With the use of this method, the lumen of a bovine aorta was filled with wash medium and the outer surface of the sample was washed with alcohol for 30 seconds. Under a laminar flow hood, the inner surface of the sample was covered with filter paper. Collagenase type II was dripped onto the filter paper as a digestion enzyme. The digestion fluid was seeded in T25 flasks and fed with complete medium every 3 days. Results. The isolated cells were characterized by markers such as CD31, von Willebrand factor, 1,1 0 -dioctadecyl-1,3,3,3 0 ,3 0tetramethylindocarbocyanine perchlorate acetylated low-density lipoprotein and angiogenesis behavior. The purity of endothelial cells was detected by flow cytometry to be of nearly 90% purity; these results were confirmed by immunostaining. Moreover, endothelial cells formed blood vesselelike tubes in a three-dimensional environment, which is specific dynamic behavior for endothelial cells. Conclusions. The new strategy presented in the current report enables isolation of a highly pure population of endothelial cells that can survive long-term culture without inducing an overgrowth of fibroblast cells.
Native cartilage matrix derived (CMD) scaffolds from various animal and human sources have drawn ... more Native cartilage matrix derived (CMD) scaffolds from various animal and human sources have
drawn attention in cartilage tissue engineering due to the demonstrable presence of bioactive
components. Different chemical and physical treatments have been employed to enhance the
micro-architecture of CMD scaffolds. In this study we have assessed the typical effects of
physical cross-linking methods, namely ultraviolet (UV) light, dehydrothermal (DHT) treatment,
and combinations of them on bovine articular CMD porous scaffolds with three different matrix
concentrations (5%, 15% and 30%) to assess the relative strengths of each treatment. Our
findings suggest that UV and UV–DHT treatments on 15% CMD scaffolds can yield
architecturally optimal scaffolds for cartilage tissue engineering.
In this paper we describe an effective technical route for the production of porous scaffolds fro... more In this paper we describe an effective technical route for the production of porous scaffolds from microcomposite material consistent of citric acid-based polyester elastomer and hydroxyapatite microparticles with potential use in tissue engineering applications. Fabricated scaffolds were characterized by field emission electron microscopy, Fourier-transform infra-red spectroscopy, thermal gravimetric analysis and mechanical tests. We demonstrate that the chemical composition, scaffold morphology, mechanical integrity and porosity of the produced microcomposite scaffolds are strongly affected by variation of the initial hydroxyapatite concentration in the mineral-polymer mixture during the fabrication process. This polyester/mineral scaffold exhibits versatility in compression modulus and strength in the range of 21.72-33.25 KPa and 49.92-101.48 KPa respectively, corresponding to the hydroxyapatite concentration in the composite (10-30 wt.%).