Ameneh Sazgarnia | Mashhad University of Medical Sciences, Mashhad. Iran. (original) (raw)

Papers by Ameneh Sazgarnia

Magnetic Resonance Materials in Physics, Biology and Medicine

Iranian Journal of Medical Physics, 2021

Objectives: Photodynamic therapy (PDT) can be considered as a non-invasive method for cancer trea... more Objectives: Photodynamic therapy (PDT) can be considered as a non-invasive method for cancer treatment. One of the most commonly used photosensitizer (PS) is Indocyanine Green (ICG), a water-soluble dye with high penetration depth. However, high ‎cytotoxicity in high concentration and instability in aqueous media were limited its application. ‎It was shown that using nanoparticles or plant extracts in combination with PS could improve PDT efficiency. In this study, anti-cancer properties of crocetin (Crt) loaded PLGA (Poly lactic-co-glycolic acid) nanoparticles (NPs) were utilized to increase the PDT efficacy with ICG on the MCF-7 cells. Materials and Methods: Crt was encapsulated into PLGA NPs and its particle size distribution and encapsulation efficiency were evaluated. IC10 of Crt, PLGA-Crt NPs and ICG was determined by MTT assay in MCF-7 cancer cells. At these concentrations, the cells were pre-treated with Crt or PLG-Crt, then treated with ICG and finally exposure to near infr...

Journal of Vaccines and Vaccination, Jun 21, 2013

ijbms.mums.ac.ir Ultraviolet B efficacy in improving antileishmanial effects of silver nanoparticles

Iranian Journal of Medical Physics, 2007

Introduction: Electroporation is a practical technique used to transport the molecules across the... more Introduction: Electroporation is a practical technique used to transport the molecules across the cell membrane. The utilization of fluorescent molecules is the method widely used to evaluate the electropermeability of cell membrane as a result of pulse application. It is also possible to use mathematical methods to predict the changes caused in cell electropermeability as a result of the changes made in the pulse parameters. So the technique can be managed in such a way that the maximum permeability and minimum cell death can be achieved when desirable. Material and Methods: In this study, MCF-7 cell line of human breast adenocarcinoma tumor was used. Propidium Iodide (PI) and Fluorometry technique was used to monitor the cell electropermeability and the cell survival. The cells were incubated in PI and electrical pulses were applied affecting the cell permeability. As a result the cell membrane is disrupted releasing the fluorescent molecules affiliated with PI. The fluorescence s...

In spite of major advances in cancer chemotherapy, effective response is obtained only in a small... more In spite of major advances in cancer chemotherapy, effective response is obtained only in a small proportion of patients, which is due to acquired drug resistance of cancer cells. Ferula species are good sources of sesquiterpenes, which are naturally occurring hydrocarbons with antimicrobial, antiviral, anticancer, anti-malarial and anti-inflammatory activities. Ferutinin, is a sesquiterpene found in umbelliferae family which has shown antimycobacterial, anti-inflammatory, antifungal, and apoptosis inducing activities. In present study, ferutinin was isolated from the roots of Ferula ovina and evaluated for its possible cytotoxic effects on human NTERA2 (human teratocarcinoma) and KYSE30 (esophageal cancer) cell lines. To determine IC50 values of this compound NTERA2 and KYSE30 cells were treated with different concentrations of ferutinin (5-100 μg/ml). After 24, 48 and 72 h cell viability was quantified by MTT assay and morphological changes were observed under a microscope. In ord...

Iranian Journal of Medical Physics, 2008

Introduction: As a physical treatment, electrochemical therapy (ECHT) has provided an effective a... more Introduction: As a physical treatment, electrochemical therapy (ECHT) has provided an effective and useful approach for treating localized tumors. The chemical changes due to a direct electric current destroy the tumor. This study evaluates the effect of electrical dose and polarity on the efficacy of the treatment of a colon tumor model. Materials and Methods: In order to induce tumors, 5 × 10 5 CT26 cells were injected into the right dorsal side of 100 Balb/c mice subcutaneously. When the mean volume of the tumors reached 350 ± 50 mm 3 , they were randomly divided into 2 control and 8 test groups, each consisting of 10 mice. Six platinum electrodes (3 cathodes and 3 anodes) were inserted into the base and the apex of each tumor. Electrical doses of 0, 10, 20, 30 or 40 C/cm 3 were then applied. Tumors volumes were subsequently measured daily and the mortalities were recorded. A histopatholological study was also performed. Results: After ECHT, a decrease in...

Iranian Journal of Biotechnology, 2010

This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (D... more This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (DC) derived from esophageal squamous cell carcinoma (ESCC) patients. Applying an electroporation technique, in vitro synthesized Green Fluorescent Protein (GFP) mRNA was transfected as an indicator into the DCs derived from a healthy donor. Flow cytometry revealed 84.9% transfection efficiency for DCs transfected with GFP mRNA. Optimized condition (500V/300 μs) yielded 79.8% efficiency in transfecting GFP mRNA into DCs from ESCC patient. Applying this efficient method, tumoral mRNA was transfected into DCs. T cells were then primed with tumor RNA/DCs and cytotoxicity assay revealed significantly higher lyses of tumor/DC vs. Mock DC; approving the optimized method for further establishment of the preclinical phase of DC-based immunotherapy for ESCC.

Photodiagnosis and Photodynamic Therapy, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Photodiagnosis and Photodynamic Therapy, 2019

nanopolymer without MX. The level of free radicals produced by nanopolymer was significantly incr... more nanopolymer without MX. The level of free radicals produced by nanopolymer was significantly increased during irradiation with X-rays. The highest mean of synergistic indexes was observed in MIP. Conclusion: The higher level of hydroxyl free radicals in MIP and lower cell viability in the DFW cell line as well as enhanced treatment efficiency confirm the hypothesis regarding the production of photodynamic effects by synthesized nanopolymer during radiotherapy.

Iranian Red Crescent Medical Journal, 2016

Background: Ionizing radiation has become an essential part of modern medicine. However, applying... more Background: Ionizing radiation has become an essential part of modern medicine. However, applying the effective dose of ionizing radiation is compromised by its inevitable radiation-induced damage to normal tissues. Natural products with free radical scavenging, antioxidant activities, and immune stimulatory effects offer suitable radio-protective effects. Objectives: This study was designed to evaluate the in vivo radio-protective activity of lycopene, a naturally dietary carotenoid, against whole-body gamma-irradiation-induced mortality and sickness in mice. Methods: In this animal experimental study, lycopene was extracted from tomato paste, characterized, and then administered to mice. Consecutively, 80 healthy adult male mice were recruited and irradiated individually, and then monitored for irradiation sickness and survival parameters. Results: Pretreatment with 7 mg/kg dose of lycopene 7 days before irradiation was found to reduce the severity of symptoms of radiation sickness in a 30-day post-radiation monitoring. Lycopene treatment increased the survival rate. At the radiation dosage of 8 and 9 Gy, the difference between the survival rates of control and sample groups became significant after day 15 and 25. Lycopene was also shown to have a protective effect on white blood cell count against 8 and 9 Gy gamma radiations. Conclusions: Natural and exhibiting whole-body radio-protective activity, lycopene can be proposed as an effective radio-protector in healthy people who are exposed to radiation, such as radiographers, nuclear pharmacists, and radiologists.

Introduction: Instruments and equipments used in dentistry field produce sounds with wide spectru... more Introduction: Instruments and equipments used in dentistry field produce sounds with wide spectrum, so dentists and other personnel are exposed to these sounds continuously. The purpose of this study was measuring the received noise level of personnel working in clinics of Mashhad dental school and frequency analysis of sounds at 20-20000 Hz frequency range. Materials and Methods: In this study, the noise level in operational dentistry, endodontics, prosthodontics, oral surgery, periodontics, pediatric dentistry and orthodontics of Mashhad dental school were assessed in 2004. Sound level measuring device and their establishment and set up were selected in which according to acquired data, the received noise level of dentist and average received noise level of personnel could be calculated. Results: Equivalent noise level (LAeq) measured surrounding central unit of each clinic, was less than 73.2 dB and noise level inside each clinic was less than 69.9dB. The dentists received noise level during work and presence at the studied clinics was measured to be 67.2-83.7 dB. The equivalent maximum noise level in all clinics was related to frequency band of 630 Hz and sounds of operational dentistry, endodontics and periodontics clinics had strong components in high frequency. Conclusion: According to permitted exposure noise level, the received noise level of dentists and personnel in studied clinics were not hazardous, but because of high frequency sounds, it is suggested to repeat the study with systems which can assess analysis of higher frequencies.

Iranian Journal of Medical Physics, 2005

Introduction: Among the environmental pollutions, noise is very important for its physiologicalÂ ... more Introduction: Among the environmental pollutions, noise is very important for its physiologicalÂ and psychological effects on human. Traffic noise is one of the most important pollutants and theÂ hospitals Â are Â one Â of Â the Â critical Â places Â regarding Â this Â type Â of Â noise. Â For Â these Â reasons, Â in Â theÂ summer Â of Â 1382, Â the Â traffic Â noise Â of Â Mashhad Â main Â streets Â around Â the Â hospitals Â was Â assessedÂ during the busiest hours.Â Materials and Methods: The noise indexes such as L Aeq , L Afmax , L 10 , L 50 Â and L 90 Â were measuredÂ by a Sound-Level-Meter, model Investigator 2260. The traffic load was also determined. On theÂ basis Â of Â these Â results, Â Noise Â Pollution Â Level Â (NPL) Â and Â Traffic Â Noise Â Index Â (TNI) Â wereÂ calculated. The assessment was done during three different periods of the days in twelve stations. Â Results: Based on the obtained results, the maximum L Aeq Â was recorded on Bahar Street duringÂ the morni...

Nanomedicine Journal, 2019

The application of nanotechnology in medicine involves using nanomaterials to develop novel thera... more The application of nanotechnology in medicine involves using nanomaterials to develop novel therapeutic and diagnostic modalities. Given the unique physiochemical and optical properties of gold nanoparticle (GNP) such as good biocompatibility, nontoxic nature, surface properties and comparative stability, it has been widely studied in medicine, especially as a cancer theranostic agent. This review focuses on recent progresses in the field of gold nanostructures in cancer treatment and diagnosis. As far as cancer detection is concerned, several studies have indicated that GNPs can be used for X-ray, MR and optical imaging. With regard to cancer treatment, most studies have investigated the effect of GNPs in different treatment modalities like photothermal therapy, photodynamic therapy, sonodynamic therapy, drug delivery, and radiotherapy. In this paper, we have focused on reviewing the role of GNPs in improving radiotherapy efficiency as radiosensitizers. For optimization of paramete...

Iranian Journal of Medical Physics, 2018

Introduction: Hollow gold nanoshells (HAuNS) are one of the most attractive nanostructures for bi... more Introduction: Hollow gold nanoshells (HAuNS) are one of the most attractive nanostructures for biomedical applications due to their interesting physicochemical properties. This study sought to evaluate the plasmonic photothermal effect of HAuNS irradiated with incoherent light on melanoma cell line. Materials and Methods: After the synthesis of nanostructures, the temperature changes of HAuNS and polyethylene glycol stabilized HAuNS (HAuNS-PEG) were evaluated at different irradiation dose levels. After determining the potential cytotoxicity of the agents, the DFW cells were irradiated by incoherent light with and without the nanostructures at different exposure doses with two spectral bands of 670±25 nm and 730±25 nm. Finally, the rate of the cell survival was determined by 1-Methyltetrazole-5-Thiol assay 24 h after irradiating. Results: The HAuNS, HAuNS-PEG, and light exposure did not have any significant effect on the cell survival, individually. Stabilizing with PEG led to an inc...

Introduction: Hyperthermia created by microwave (MW), infrared, ultrasound and other methods, is ... more Introduction: Hyperthermia created by microwave (MW), infrared, ultrasound and other methods, is often utilized as an adjuvant to sensitize cancer cells to the effects of chemotherapy and radiation therapy. We investigated the efficacy of hyperthermia using MW in synergy with chemotherapy in the presence and absence and gold nanoparticles (GNPs). Material and Methods: After culturing and proliferation of the Saos-2 cell line derived from human osteogenic sarcoma, the cells were incubated at two concentrations of GNPs in two diameters of 20 and 40 nm and in the absence and presence of doxorubicin in different groups. Forty eight hours after irradiating the cells with MW up to a temperature of 42°C, cell survival rate was determined using the MTT method, in order to study the effectiveness of the therapeutic parameters. Results: Cell survival in the presence of GNPs was greater than 95%. After chemotherapy by doxorubicin with and without 40 nm GNPs, cell survival rates were determined...

Introduction: Photodynamic therapy is a noninvasive therapeutic method for tumors with a maximum ... more Introduction: Photodynamic therapy is a noninvasive therapeutic method for tumors with a maximum depth of 5 mm. On the other hand, most photosensitizers are also susceptible to ultrasound waves (the basis of sonodynamic therapy). Therefore, it is expected that a combination of the two therapeutic methods will increase effectiveness of photodynamic therapies for lower doses of sensitizer and curing deeper tumors. This study evaluates the synergistic effects of photodynamic and sonodynamic therapies. Materials and methods: The study was conducted on a colon carcinoma tumor model in Balb/c mice. The colon carcinoma tumors were induced in the mice by subcutaneous injection. Twenty four hours after intraperitoneal injection of Zinc Phthalocyanine liposome as a sensitizer, at first ultrasound irradiation with a known frequency and intensity was performed followed by illumination of the tumor area. Evaluation of the treatment efficacy was done using daily measurement of the tumors and cal...

Photodiagnosis and Photodynamic Therapy, 2021

BACKGROUND Cold Atmospheric Plasma (CAP) has proposed as a new approach based on its anticancer p... more BACKGROUND Cold Atmospheric Plasma (CAP) has proposed as a new approach based on its anticancer potential. However, its biological effects in combination with other physical modalities and also enhance efficiency and expand the applicability of the CAP method remain elusive. On the other hand, Photodynamic Therapy (PDT) has been partially improved by the use of indocyanine green (ICG) photosensitizer with absorption wavelength in the near infrared region. In this study, the effectiveness of cold atmospheric helium plasma (He-CAP) as a pretreatment on the efficiency of ICG mediated PDT was investigated. METHODS AND MATERIAL First, toxicity of different concentrations of ICG on HT-29 and U-87MG cell lines was examined for 24 h. IC10 and IC30 of ICG were determined and then cells were treated with this ICG concentrations with different plasma radiation doses and light exposures for 48 h. Finally, MTT assay was performed for all treatment groups. The experiments were repeated at least 4 times at each group for two cell lines, separately. In order to compare the results, several indicators such as treatment efficiency, synergistic ratio, and the amount of optical exposure required for 50% cell death (ED50) were also defined. Finally, SPSS 20 software is used for statistical analysis of data. RESULTS Pretreatment with CAP could significantly reduce cell survival in both cell lines (P<0.05). Also concentrations, irradiation time with CAP, and appropriate light exposure in both cell lines increased therapeutic efficiency compared to either treatment alone (P<0.05). While increasing the efficiency of photodynamic therapy varied between the two cell lines, the improvement in the PDT process was demonstrated by pretreatment with CAP. CONCLUSION Synergistic effect in the cell death with PDT were observed following He-CAP treatment and the results indicated that pretreatment with He-CAP improves the efficiency of photodynamic therapy.