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Papers by Samaneh Mollazadeh

Http Dx Doi Org 10 1080 10286020 2010 485565, Jul 1, 2010

Journal of Asian Natural Products Research, 2010

Bladder cancer is the second common cancer of the genitourinary system throughout the world and i... more Bladder cancer is the second common cancer of the genitourinary system throughout the world and intravesical chemotherapy is usually used to reduce tumour recurrence and progression. Human transitional cell carcinoma (TCC) is an epithelial-like adherent cell line originally established from primary bladder carcinoma. Here we report the effect of mogoltacin, a sesquiterpene coumarin from Ferula badrakema on TCC cells. Mogoltacin was isolated from the fruits of F. badrakema, using silica gel column chromatography and preparative thin layer chromatography. Mogoltacin did not have any significant cytotoxicity effect on neoplastic TCC cells at 16, 32, 64, 128, 200 and 600 mg ml À1 concentrations. In order to analyse its combination effect, TCC cells were cultured in the presence of various combining concentrations of mogoltacin and vincristine. Cells were then observed for morphological changes (by light microscopy) and cytotoxicity using MTT assay. The effect of mogoltacin on vincristine toxicity was studied after 24, 48 and 72 h of drug administration. The results of MTT assay showed that mogoltacin can significantly enhance the cytotoxicity of vincristine and confirmed the morphological observations. Results revealed that combination of 40 mg ml À1 vincristine with 16 mg ml À1 mogoltacin increased the cytotoxicity of vincristine after 48 h by 32.8%.

Bladder cancer is one of the most common cancers worldwide, with the highest incidence in indust... more Bladder cancer is one of the most common cancers worldwide, with the highest incidence
in industrialized countries. There are three major histological subtypes of bladder cancer:
transitional cell carcinoma (TCC) (>90%), squamous cell carcinoma (<10%) and adeno-
carcinoma (1 – 2%). The present study was carried out to assess the effects of conferone, a
sesquiterpene coumarin isolated from Ferula badrakema, on a TCC subline, 5637 cells. In or-
der to test the effects of conferone, 5637 cells were treated with different concentrations (16,
32, 64, 128 μg/ml) of conferone. The results indicated that conferone did not have any signiﬁ -
cant cytotoxic effect on these neoplastic cells. To determine the combining effects, the cells
were cultured in the presence of different concentrations of conferone (16, 32, 64, 128 μg/
ml) and vincristine (30, 40, 50 μg/ml) in combination. The morphological changes were then
observed and cytotoxicity effects were studied using the MTT assay 24, 48 and 72 h follow-
ing drug administration. The cells were more rounded and granulated after treatments with
both drugs in comparison to vincristine only. The results of the MTT assay conﬁ rmed the
morphological observations. After 48 h of combined treatment with 40 μg/ml vincristine and
16 μg/ml conferone, the cytotoxicity of vincristine was increased by 23.6%.
Key words: Conferone, Vincristine, 5637 Cell Line

Transitional cell carcinoma (TCC), which is the most common type of bladder cancer, shows resist... more Transitional cell carcinoma (TCC), which is the most common type of bladder cancer,
shows resistance to chemotherapeutic agents due to the overexpression of drug efﬂ ux
pumps. In this study, the effects of feselol, a sesquiterpene coumarin extracted from Ferula
badrakema, on cisplatin cytotoxicity were investigated in 5637 cells, a TCC subline. Cell vi-
ability and DNA lesion were evaluated by thiazolyl blue tetrazolium bromide and comet
assays, respectively. Feselol had no signiﬁ cant cytotoxic effect on 5637 cells but at 32 μg/mL
it increased the cytotoxicity of 1 μg/mL cisplatin by 37% after 24 h. Furthermore, the comet
assay revealed that DNA damage induced by cisplatin on 5637 cells is enhanced by 31%
when used in combination with feselol. Therefore, feselol might be considered as an effective
reversal agent for future in vivo and clinical studies.
Key words: Feselol, Cisplatin, 5637 Cells

Urinary bladder cancer is one of the most common cancers worldwide. Human transitional cell carci... more Urinary bladder cancer is one of the most common cancers worldwide. Human transitional cell carcinoma (TCC) cells are epithelial-like adherent cells originally established from a primary bladder carcinoma. Studies have shown that TCC cells are resistant to some chemotherapeutic agents such as vincristine (VCR). In the present study, the effect of feselol, a sesquiterpene coumarin isolated from the fruits of Ferula badrakema, was investigated on VCR effectiveness. Our results demonstrated that feselol itself did not have any cytotoxic effect on TCC cells. In order to check its combinatorial effects, TCC cells were exposed to various combined concentrations of feselol and VCR. Then, morphological changes were monitored and cytotoxicity was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay for three consequent days. Results showed that the combination of 40 mg/ml VCR with 16 mg/ml feselol increased the cytotoxicity of VCR by 28.32% after 48 h. This effect might be due to inhibition of P-glycoprotein in TCC cells by feselol.

In this article, bone cells and their intercellular communications have been reviewed. Gap juncti... more In this article, bone cells and their intercellular communications have been reviewed. Gap junctions and hemichannels are the main routes of interactions in bone tissue. They play a substantial role in survival and cell death, since pro-apoptotic signals can propagate through them. Different adhesion molecules are required for apoptosis, particularly caspase family as well as noncaspase proteases. The disruption outcome of apoptosis could result in bone-related diseases such as osteonecrosis. Anti-apoptotic strategies include inhibition of caspase, poly [ADP-ribose] polymerase (PARP), and Bcl-2 proteins as well as induction of the PKB/Akt pathway and inhibitors of apoptosis (IAP) family of proteins. Thus, understanding the mechanism of apoptosis gives detailed insights of anti-apoptotic molecular targets. Based on these targets, different treatments were designed and produced such as estrogen replacement therapy, administration of different bisphosphonates, raloxifene, calcitonin, sodium fluoride, calcium, and vitamin D. As a result, new applicable drugs for treatment of related bone problems can be proposed for clinical approach especially in the early stage of diseases.

Introduction: Ciprofloxacin which was used in this study is a Fluoroquinolone (FQ). This kind of ... more Introduction: Ciprofloxacin which was used in this study is a Fluoroquinolone (FQ). This kind of drug may cause epileptic seizures probably because of the inhibition of GABA binding to its receptors. Wag/Rij rats (an animal model for generalized absence epilepsy), were used as experimental subjects. Methods: For EEG study, electrodes were inserted into the cortex of animals according to paxinos coordinates. After and before ciprofloxacin injection, EEG was recorded and their SWDs were compared with each others. Results: Findings showed a significant increase in the mean number of seizures during recording period. But the mean number of SWDs during seizures did not show any significant differences between groups. Discussion: These results may be due to involvement of GABA antagonistic effects of FQs and/or Mg2+ linked blockade of NMDA receptors. More researches are going to determine physiopathology of SWDs and find new effective substance against this kind of epilepsy.

Other by Samaneh Mollazadeh

Transitional cell carcinomas (TCCs), which account for 90% of bladder cancers, arise from the tra... more Transitional cell carcinomas (TCCs), which account for 90% of bladder cancers, arise from the transitional epithelium of bladder. Cisplatin is a chemotherapeutic drug used to treat bladder cancer, but intrinsic and acquired resistance to cisplatin limit its effectiveness. The aim of this study was to determine the ability of mogoltacin, a sesquiterpene-coumarin from Ferula badrakema, to enhance cytotoxic effects of cisplatin on 5637 cells, using MTT assay, comet method, DAPI staining and efflux assay. In order to analyse mogoltacin combinatorial effects, 5637 cells were cultured in the presence of various combined concentrations of mogoltacin and cisplatin. The results of MTT assay revealed that combination of 1 μg/mL cisplatin+32 μg/mL mogoltacin, increased the cytotoxicity of cisplatin by 45.3%. Investigating the mechanism of this action by comet assay indicated that mogoltacin increases the apoptotic effects of cisplatin on 5637 cells via DNA lesion by 44%. Furthermore, studying nuclear morphological changes revealed that the combination of mogoltacin+cisplatin significantly (P<0.001) increases the number of apoptotic cells. Results of efflux assay indicated that mogoltacin did not have any significant effect on the activity of MDR transporters, therefore, this sesquiterpene-coumarin increases the effects of cisplatin possibly by interacting with other drug transporters.

Http Dx Doi Org 10 1080 10286020 2010 485565, Jul 1, 2010

Journal of Asian Natural Products Research, 2010