Amrutha Audipudi | Acharya Nagarjuna University (original) (raw)
Papers by Amrutha Audipudi
Recent trends in PGPR research for sustainable crop productivity. 4th Asian PGPR Conference Proceedings, Hanoi, Vietnam, 3-6 May, 2015, 2016
Biochemical and Biophysical Research Communications, 2000
Twenty three bacterial strains isolated from the soil of Chollangi mangrove environment and demon... more Twenty three bacterial strains isolated from the soil of Chollangi mangrove environment and demonstrated Phosphate solubilization potential of isolated bacterial strains. Seven isolates showed phosphate solubilization activity. Morpho physiologically and biochemically identified two isolates (CMB1 and CMB2) as Bacillus subtilis, three isolates (CMFP3, CMFP4 and CMFP5) as fluorescent Pseudomonas and two isolates (CMAZ1 and CMAZ2) as Azotobacter sp. Plant growth promoting ability of seven bacterial strains tested for optimization of P H , carbon source and temperature and estimated phosphate solubilization quantitatively. Isolated strains visualized colonies at P H 8.6 after 72 hrs, where as pure cultures visualized early within 18 hrs at P H 6.8-7.2 range. Observations indicated bacterial isolates of arid mangrove environment can easily adapted to normal soil P H and enhance phosphate content in soil.
Journal of advanced scientific research, May 31, 2021
Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an econom... more Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an economical technique, which is employed as an efficient tool for microorganism identification and FTIR spectrum obtai ned for any identified compound gives the information on the unique fingerprint. During this study, an effort was made to interpret FTIR spectral data in differentiating the ability of antagonism of endophytic bacteria against Fusarium oxysporium var. lycopersii, a phytopathogen of tomato. Antagonism of chilli endophytic bacteria Pseudomonas aeruginosa AVSCE-1 (FR3) against Fusarium oxysporum-MTCC10270 (FO) were studied by dual culture method. FR3 showed significant antagonism against the growth of FO with inhibition of 62.5%. FTIR spectra of ethyl acetate extracts of FO, FR3 and FO+FR3 were analyzed. In FTIR analysis,
Arabian Journal of Chemistry, Feb 1, 2022
Research Square (Research Square), Feb 22, 2023
A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole co... more A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole compounds via an e cient, one-pot, pseudo four-component reaction using 5-amino-2mercaptobenzimidazole, aralkyl halides, ammonium thiocyanate, and substituted α-bromoacetophenones in glacial acetic acid at ambient temperature to give nal compounds (4a-p) in good yields in shorter time. The spectral data of synthesized compounds were evaluated by analytical and spectral techniques (IR, 1 H-NMR, 13 C-NMR, and ESI-HRMS). Further, some of the synthesized compounds were screened for their in-vitro antibacterial activity studies using the agar well diffusion method against Gram-positive Streptococcus Pneumoniae (2451) bacteria and Gram-negative Porteous Mirabilis (2081) bacteria. Based on the MIC results, it was observed that the most active compounds 4b, 4e, 4f, and 4k are shown promising anti-bacterial activity with the zone of inhibition values of 2.85 cm 2.75 cm, 3.6 cm, and 3.3 cm against both Gram-negative and Gram-positive bacteria cell lines respectively. Further, we have also insight into the molecular simulation studies, based on the binding results, compound 4i showed stable binding interactions with streptomycin drug with active site of the gyrase protein (PDB ID: 1KIJ). The structure-activity relationship (SAR) studies of all the title scaffolds were also established. The antibacterial activity, molecular docking studies, molecular dynamic simulations of the title compounds were suggested that these are promising anti-bacterial active skeletons. Ethanol NaOH 60 12 43 Ethanol KOH 60 12 45 Ethanol Na 2 CO 3 60 12 35 Ethanol K 2 CO 3 60 12 42 Ethanol Et 3 N 60 12 41 Ethanol Acetic acid 60 10 52 Ethanol Acetic acid 60 6 60 AcOH-60 4 65 AcOH AcONa (1.0 mmol) 60 4 AcOH AcONa (2.0 mmol) 70 4 88 AcOH-re ux 4 51
Journal of advanced scientific research, Apr 30, 2022
From decades, viral diseases including emerging and chronic viral infections are increasing world... more From decades, viral diseases including emerging and chronic viral infections are increasing worldwide health concern. Corona virus, SARS COV-2, is a new strain identified in 2019 caused Covid-19, in Wuhan, China has caused more than 5,304,772 infections and ~342,029 deaths worldwide over 203 countries, and the numbers are increasing exponentially from time to time. Since no specific treatment and diagnosis are available for COVID-19, discovery of new antiviral agents have become the most urgent need than in past, especially from natural sources. Hence, our present study is aimed to investigate bioactive molecules from Zingiber officinale (ginger) and Allium sativum (garlic) as potential SARS COV-2 main protease inhibitors, using a molecular docking by Auto dock 4.2, with the Lamarckian Genetic Algorithm, to analyze the probability of docking. SARS COV-2 main protease was docked with five selected compounds (zingerone, gingerol, paradol, dichloro flavin (ginger), and allicin (garlic), and were analyzed by Auto dock 4.2, PyMol version 1.7.4.5. Chloroquine and hydroxy chloroquine were used as standards for comparison. The binding energies obtained from the docking of Covid-19 main protease with biological ligand; zingerone, gingerol, paradol, dichloro flavin, and allicin were-5.31,-4.73,-5.51,-6.05,-3.74 kcal/mol, while, standard drugs such as hydroxy chloroquine and chloroquine possess binding energies of-4.88 and-5.25 kcal/mol respectively. It indicates that, zingerone, paradol and dichloloroflavan show more binding energy than chloroquine and hydroxy chloroquine, whereas, gingerol and allicin exhibited lesser binding energy. Comparing these five molecules, dichloroflavan and paradol from ginger shows highest affinity and binding sites for target protein.
Research Square (Research Square), Mar 15, 2022
A non-uorescent pigment produced from the uorescent Bacillus endophyticus AVP-9 which possess abu... more A non-uorescent pigment produced from the uorescent Bacillus endophyticus AVP-9 which possess abundant lutein content considered as a xanthophyl-type compound with potentially bene cial pharmacological properties for enhancing human health. In this context, bacterial lutein was extracted by employing different solvents to isolate from Bacillus endophyticus was examined in this study. Chromatographically puri ed lutein was spectrally characterized using ultraviolet-visible spectrometry and spectral analysis was carried through liquid chromatography-mass spectrometry, functional group identi cation was done by using fourier transform-infrared spectroscopy, structural determination by nuclear magnetic resonance spectrometry. By using hexane and ethyl acetate (7:3) with the ow rate of 5ml/min, was proven to be an excellent solvent phase for separation of lutein, meaning that up to 90% pure lutein generated with a 60% estimated yield. The puri ed compound identi ed as a β, ε-3, 3 1-diol (lutein) having molecular weight of 568.6g/mol with the molecular formula C 40 H 56 O 2 which exhibits potential antibacterial activity against E. coli (35mm) followed by S. typhi (33mm), S. exneri (30mm), P. aureginosa (29mm), M. lutea (28mm), P. vulgaris (24mm), S. aureus (21mm) and lowest antibacterial activity against V. cholera (19mm). Puri ed pigment shows concentration dependent antioxidant activities with IC 50 values of pigment was found to be 4.5 mg/ml. Whereas anti-proliferation activity pigment shows considerable effect MCF-7 and LNCaP cell lines, when treated with 100µg/ml, cell viability was decreased to 36.363% at IC 50 value of 21.935 µg/ml and for LNCaP, the cell viability was decreased to 42.449% at IC 50 value of 56.937 µg/ml.
A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole co... more A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole compounds via an efficient, one-pot, pseudo four-component reaction using 5-amino-2-mercaptobenzimidazole, aralkyl halides, ammonium thiocyanate, and substituted α-bromo-acetophenones in glacial acetic acid at ambient temperature to give final compounds (4a-p) in good yields in shorter time. The spectral data of synthesized compounds were evaluated by analytical and spectral techniques (IR, 1H-NMR, 13C-NMR, and ESI-HRMS). Further, some of the synthesized compounds were screened for their in-vitro antibacterial activity studies using the agar well diffusion method against Gram-positive Streptococcus Pneumoniae (2451) bacteria and Gram-negative Porteous Mirabilis (2081) bacteria. Based on the MIC results, it was observed that the most active compounds 4b, 4e, 4f, and 4k are shown promising anti-bacterial activity with the zone of inhibition values of 2.85 cm 2.75 cm, 3.6 cm, and 3.3 cm again...
Journal of Advanced Scientific Research
Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an econom... more Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an economical technique, which is employed as an efficient tool for microorganism identification and FTIR spectrum obtained for any identified compound gives the information on the unique fingerprint. During this study, an effort was made to interpret FTIR spectral data in differentiating the ability of antagonism of endophytic bacteria against Fusarium oxysporium var. lycopersii, a phytopathogen of tomato. Antagonism of chilli endophytic bacteria Pseudomonas aeruginosa AVSCE-1 (FR3) against Fusarium oxysporum- MTCC10270 (FO) were studied by dual culture method. FR3 showed significant antagonism against the growth of FO with inhibition of 62.5%. FTIR spectra of ethyl acetate extracts of FO, FR3 and FO+FR3 were analyzed. In FTIR analysis, C-O and C-C stretch of chitin at 1073.35 cm-1, C-N stretch of Amide II at 1232.97 cm-1, C-O stretch of Amide III at 1300 cm-1 and C=O stretch of lipid at 1650-18...
Int. J. of Aquatic Science, Jun 1, 2021
It turns out that there is a tremendous amount of wealth below the surface of the ocean waiting t... more It turns out that there is a tremendous amount of wealth below the surface of the ocean waiting to be discovered. The ocean environment is capable of solving many unsolved questions and is also home to an undiscovered hoard of mysteries. Seawater collected from Suryalanka marine sediments in Guntur, Andhra Pradesh, India was used to extract coastal soil samples. Selected bacterial strains were isolated using the spread plate technique, the bacteria with biological activity were screened using antibacterial activity, and the 16S rRNA sequencing was utilised to identify the bacteria. Metabolite which is biologically active was created in a defined setting (incubation, temperature, pH, Nacl concentration, and carbon amino acid and nitrogen sources). TLC was used to purify crude extract, which was then eluted with hexane to separate it on a column. Spectrally characterised purified fraction was examined for antibacterial and anticancer properties. Out of the 23 isolates, isolate AVSC3 exhibits a noticeable inhibitory activity against test organisms. This was established through a characterization of the test organism, Bacillus subtilis AVSC3, with a GenBank accession number of GEO:M21745. MN386243. To produce bioactive metabolites, an ideal incubation time is 60 hours, 35°C, pH 7, 0.5% NaCl, and 1% glucose and peptone. The antibacterial property of the pure fraction was found to be hexadecanoic acid, with a molecular formula of C16H32O2, which has spectral characteristics characteristic of this molecule. 14.2 mm-long Coli followed by Staphylococcus The 13.7-mm-diameter aureus, the 9-mm-diameter M.luteus, the 8.1-mm-diameter S.pyogenes, the 4.6-mm-diameter P. vulgaris, and the 4.4-mm-diameter S.typhi all demonstrated significant antimicrobial activity against MCF-7 cell lines, with the strongest effect observed at 25µg.
Int.J.Curr.Microbiol.App.Sci, 2017
L-asparaginase is used in the treatment of cancer, especially for acute lymphoblastic leukemia. B... more L-asparaginase is used in the treatment of cancer, especially for acute lymphoblastic leukemia. Bacterial species were isolated from the Nizampatnam mangrove sample and screened for its ability to produce the extracellular Lasparaginase enzyme. Effects of pH and temperature were investigated in the production of enzymes. The highest yield of the enzyme was observed when the pH of the fermentation cycle raised above 9 at 37°C. Effective production was observed while using Dextrose and Peptone as carbon and nitrogen source.
Recently there has been an explosion of information about novel bioactive compounds that have bee... more Recently there has been an explosion of information about novel bioactive compounds that have been isolated from marine microbes in an effort to further explore the relatively untapped marine microbes and their secondary metabolites for drug discovery. New therapeutic agents are urgently needed to treat medicinal needs that are currently unmet .The biodiversity of marine microbes and the versatility of their bioactive metabolites has not been fully explored. L-asparaginase therapy, alone is finding increased success in the management of acute lymphocytic leukemia's. Large amounts of purified enzyme and increasing recognition of successful therapy will generate increased demand. The production of L-asparaginase by using bacteria has attracted great attention owing to their cost effective and eco-friendly nature. In search of efficient microorganisms potential for production of anticancer enzymes, bacteria isolated from mangrove soil of Nizampatnam Guntur district were screened fo...
Arabian Journal of Chemistry, 2021
... 1) 2011 ISSN: 2231-2560 Research Article 659 Asian Journal of Biochemical and Pharmaceutical ... more ... 1) 2011 ISSN: 2231-2560 Research Article 659 Asian Journal of Biochemical and Pharmaceutical Research Clinical Manifestation & Novel Targets for HIV Therapy Niyaz Alam*, Alok Bhardwaj, Faisal Amin, Vivek Dabas, . Rameesh ...
Microorganisms are good sources for the production of biologically active secondary metabolites (... more Microorganisms are good sources for the production of biologically active secondary metabolites (Monaghan RL et al., 1990). Bacteria belonging to the genus Bacillus are among the most widely distributed microorganisms have produced antibiotics in the soluble protein structure and that these antibiotics have been found to be cheaper and more effective, these microorganisms are preferable for commercial production (Priest FG et al., 1989). Also, Strains of Bacillus are known producers of bioactive cyclic lipopeptides (Konz D et al., 1999). Bacillus has been investigated for their ability to produce so called bacteriocin like inhibitory substance BLIS. It has been reported that strains of B. thuringiensis, B. subtilis, B. stearothermophilus, B. licheniformis, B. megaterium and B. cereus produce BLIS (Motta AS et al., 2007). On the other hand, Bacillus subtilis showed a production of a large number of antibiotics, which are classified as non-ribosomal (Stein T et al., 2005). In another ...
World Journal of Dentistry
ORIGINAL RESEARCH agents include various antimicrobial agents, out of which mouth rinses are part... more ORIGINAL RESEARCH agents include various antimicrobial agents, out of which mouth rinses are particularly well accepted due to their ease of use and ability to reach places that are difficult to clean with a toothbrush. 6 Chlorhexidine mouth rinses (0.2%), among a myriad of oral hygiene products, have acquired an eponym of "gold standard in plaque control" owing to their remarkable therapeutic effect and substantivity. It is also accompanied by some disquieting characteristics such as extrinsic staining of teeth and tongue by attaching to the IntroductIon Oral health has a significant impact on general and oral health-related quality of life. Dental caries is one of the most prevalent, biofilm mediated, sugar-driven, and multifactorial dynamic diseases affecting most of the population, regardless of age, sex, or race. 1 Worldwide, approximately 36% of the population has dental caries in their permanent teeth, and in primary teeth, the incidence is about 9%. The overall prevalence rate of dental caries in children worldwide is 46.2% in primary teeth, whereas it is about 53.8% in permanent teeth. The reasons could be due to changes in dietary habits, illiteracy, improper oral hygiene practices, a dearth in the awareness on the significance of primary teeth, deficiency of conveyance facilities, and access to oral healthcare services. 2,3 Dental plaque is a major etiological factor in the initiation and progression of oral diseases such as dental caries and gingivitis. Plaque has a diverse microbial composition, out of which S. mutans is considered to be the primary contributing factor responsible for the initiation of dental caries. 4 Therefore, caries control programs include chemoprophylactic agents that target S. mutans. Plaque control can be accomplished either mechanically or chemically or by a combination of both. Mechanical plaque control includes tooth brushing and flossing. However, it may be limited by several factors, such as lack of time, poor manual dexterity skills, or deficient motivation. 5 Hence, a customary approach is to support mechanical plaque removal with a chemotherapeutic agent. Chemically available plaque control 1-4
Journal of microbiology, biotechnology and food sciences, 2019
Antiproliferative secondary metabolites producing bacterial strain AVSC4 isolated from marine sed... more Antiproliferative secondary metabolites producing bacterial strain AVSC4 isolated from marine sediments was identified as Bacillus flexus based on 16S rRNA gene sequence analysis. Under the strategy of liquid- liquid extraction, the crude extract was obtained showed significant antibacterial activity against different clinical pathogens. 0.5% methionine and 0.4% NaCl act as inducers for maximizing the growth and antibacterial activity of strain at pH 7 and 40°C. Heptadecanoic acid and methyl hexadecanoic acid were identified as major and dominant secondary metabolites by GC-MS analysis and also showed significant antiproliferative activity against HT-29 (Human colorectal adenocarcinoma) and A-549 (Lung Cancer) with IC50 value 93.4 µg/ml and 50.04 µg/ml.
The objective of the work was to isolate and screened for phosphate solubilization, multiple plan... more The objective of the work was to isolate and screened for phosphate solubilization, multiple plant growth promoting traits and phytopathogenic antagonism from chilli rhizosphere .The isolate putatively identified as Bacillus endophyticus AVP9 on the basis of biochemical characteristics and 16srRNA partial gene sequence analysis. Acid phosphatase activity of AVP9 was also characterized for the first time and its activity in correlation with growth was studied at different PH (3.0 to 4.8), 12 different amino acids and 15 carbon sources After growth optimization Bacillus endophyticus AVP9 showed 1112ppm inorganic phosphate solubilization and multiple plant growth promoting traits indole acetic acid(80µg), ammonia (110 µg) , HCN ,catalase and siderophore production . Maximum acid phosphatase activity was found at PH 3.4(0.2IU), Fructose (0.86IU) and Mannitol (0.83IU) and no correlation was observed with growth. Multiple potential of isolate AVP9 can be exploited in agricultural and indu...
To characterize and bioprocessing of oncolytic enzyme-L-asparaginase from novel marine bacteria b... more To characterize and bioprocessing of oncolytic enzyme-L-asparaginase from novel marine bacteria by statistical optimization for maximum asparaginase production. Out of 47, 9 bacterial isolates obtained from mangrove sediments showed positive Lasparaginase activity. One of the novel marine Bacillus AVP 14 was identified as Bacillus subtilis by 16S rRNA partial sequence and characterized for growth optimization and maximum production of anticancer enzyme. Solid state fermentation of L-Asparaginase production by using sesame oil cake, Groundnut oil cake, Red gram husk, Bengal gram husk, coconut oil cake, Green tea, Coffee was also done. Bacillus subtilis AVP 14 identified as potent extracellular producer of L-Asparaginase (347.42 IU) and showed 15 folds enhancement of L-asparaginase production (5,205 U/gds) under submerged fermentation condition in 5 days in presence of inducers and activators. The results emphasizes that submerged fermentation with Red gram yields more production than...
Recent trends in PGPR research for sustainable crop productivity. 4th Asian PGPR Conference Proceedings, Hanoi, Vietnam, 3-6 May, 2015, 2016
Biochemical and Biophysical Research Communications, 2000
Twenty three bacterial strains isolated from the soil of Chollangi mangrove environment and demon... more Twenty three bacterial strains isolated from the soil of Chollangi mangrove environment and demonstrated Phosphate solubilization potential of isolated bacterial strains. Seven isolates showed phosphate solubilization activity. Morpho physiologically and biochemically identified two isolates (CMB1 and CMB2) as Bacillus subtilis, three isolates (CMFP3, CMFP4 and CMFP5) as fluorescent Pseudomonas and two isolates (CMAZ1 and CMAZ2) as Azotobacter sp. Plant growth promoting ability of seven bacterial strains tested for optimization of P H , carbon source and temperature and estimated phosphate solubilization quantitatively. Isolated strains visualized colonies at P H 8.6 after 72 hrs, where as pure cultures visualized early within 18 hrs at P H 6.8-7.2 range. Observations indicated bacterial isolates of arid mangrove environment can easily adapted to normal soil P H and enhance phosphate content in soil.
Journal of advanced scientific research, May 31, 2021
Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an econom... more Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an economical technique, which is employed as an efficient tool for microorganism identification and FTIR spectrum obtai ned for any identified compound gives the information on the unique fingerprint. During this study, an effort was made to interpret FTIR spectral data in differentiating the ability of antagonism of endophytic bacteria against Fusarium oxysporium var. lycopersii, a phytopathogen of tomato. Antagonism of chilli endophytic bacteria Pseudomonas aeruginosa AVSCE-1 (FR3) against Fusarium oxysporum-MTCC10270 (FO) were studied by dual culture method. FR3 showed significant antagonism against the growth of FO with inhibition of 62.5%. FTIR spectra of ethyl acetate extracts of FO, FR3 and FO+FR3 were analyzed. In FTIR analysis,
Arabian Journal of Chemistry, Feb 1, 2022
Research Square (Research Square), Feb 22, 2023
A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole co... more A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole compounds via an e cient, one-pot, pseudo four-component reaction using 5-amino-2mercaptobenzimidazole, aralkyl halides, ammonium thiocyanate, and substituted α-bromoacetophenones in glacial acetic acid at ambient temperature to give nal compounds (4a-p) in good yields in shorter time. The spectral data of synthesized compounds were evaluated by analytical and spectral techniques (IR, 1 H-NMR, 13 C-NMR, and ESI-HRMS). Further, some of the synthesized compounds were screened for their in-vitro antibacterial activity studies using the agar well diffusion method against Gram-positive Streptococcus Pneumoniae (2451) bacteria and Gram-negative Porteous Mirabilis (2081) bacteria. Based on the MIC results, it was observed that the most active compounds 4b, 4e, 4f, and 4k are shown promising anti-bacterial activity with the zone of inhibition values of 2.85 cm 2.75 cm, 3.6 cm, and 3.3 cm against both Gram-negative and Gram-positive bacteria cell lines respectively. Further, we have also insight into the molecular simulation studies, based on the binding results, compound 4i showed stable binding interactions with streptomycin drug with active site of the gyrase protein (PDB ID: 1KIJ). The structure-activity relationship (SAR) studies of all the title scaffolds were also established. The antibacterial activity, molecular docking studies, molecular dynamic simulations of the title compounds were suggested that these are promising anti-bacterial active skeletons. Ethanol NaOH 60 12 43 Ethanol KOH 60 12 45 Ethanol Na 2 CO 3 60 12 35 Ethanol K 2 CO 3 60 12 42 Ethanol Et 3 N 60 12 41 Ethanol Acetic acid 60 10 52 Ethanol Acetic acid 60 6 60 AcOH-60 4 65 AcOH AcONa (1.0 mmol) 60 4 AcOH AcONa (2.0 mmol) 70 4 88 AcOH-re ux 4 51
Journal of advanced scientific research, Apr 30, 2022
From decades, viral diseases including emerging and chronic viral infections are increasing world... more From decades, viral diseases including emerging and chronic viral infections are increasing worldwide health concern. Corona virus, SARS COV-2, is a new strain identified in 2019 caused Covid-19, in Wuhan, China has caused more than 5,304,772 infections and ~342,029 deaths worldwide over 203 countries, and the numbers are increasing exponentially from time to time. Since no specific treatment and diagnosis are available for COVID-19, discovery of new antiviral agents have become the most urgent need than in past, especially from natural sources. Hence, our present study is aimed to investigate bioactive molecules from Zingiber officinale (ginger) and Allium sativum (garlic) as potential SARS COV-2 main protease inhibitors, using a molecular docking by Auto dock 4.2, with the Lamarckian Genetic Algorithm, to analyze the probability of docking. SARS COV-2 main protease was docked with five selected compounds (zingerone, gingerol, paradol, dichloro flavin (ginger), and allicin (garlic), and were analyzed by Auto dock 4.2, PyMol version 1.7.4.5. Chloroquine and hydroxy chloroquine were used as standards for comparison. The binding energies obtained from the docking of Covid-19 main protease with biological ligand; zingerone, gingerol, paradol, dichloro flavin, and allicin were-5.31,-4.73,-5.51,-6.05,-3.74 kcal/mol, while, standard drugs such as hydroxy chloroquine and chloroquine possess binding energies of-4.88 and-5.25 kcal/mol respectively. It indicates that, zingerone, paradol and dichloloroflavan show more binding energy than chloroquine and hydroxy chloroquine, whereas, gingerol and allicin exhibited lesser binding energy. Comparing these five molecules, dichloroflavan and paradol from ginger shows highest affinity and binding sites for target protein.
Research Square (Research Square), Mar 15, 2022
A non-uorescent pigment produced from the uorescent Bacillus endophyticus AVP-9 which possess abu... more A non-uorescent pigment produced from the uorescent Bacillus endophyticus AVP-9 which possess abundant lutein content considered as a xanthophyl-type compound with potentially bene cial pharmacological properties for enhancing human health. In this context, bacterial lutein was extracted by employing different solvents to isolate from Bacillus endophyticus was examined in this study. Chromatographically puri ed lutein was spectrally characterized using ultraviolet-visible spectrometry and spectral analysis was carried through liquid chromatography-mass spectrometry, functional group identi cation was done by using fourier transform-infrared spectroscopy, structural determination by nuclear magnetic resonance spectrometry. By using hexane and ethyl acetate (7:3) with the ow rate of 5ml/min, was proven to be an excellent solvent phase for separation of lutein, meaning that up to 90% pure lutein generated with a 60% estimated yield. The puri ed compound identi ed as a β, ε-3, 3 1-diol (lutein) having molecular weight of 568.6g/mol with the molecular formula C 40 H 56 O 2 which exhibits potential antibacterial activity against E. coli (35mm) followed by S. typhi (33mm), S. exneri (30mm), P. aureginosa (29mm), M. lutea (28mm), P. vulgaris (24mm), S. aureus (21mm) and lowest antibacterial activity against V. cholera (19mm). Puri ed pigment shows concentration dependent antioxidant activities with IC 50 values of pigment was found to be 4.5 mg/ml. Whereas anti-proliferation activity pigment shows considerable effect MCF-7 and LNCaP cell lines, when treated with 100µg/ml, cell viability was decreased to 36.363% at IC 50 value of 21.935 µg/ml and for LNCaP, the cell viability was decreased to 42.449% at IC 50 value of 56.937 µg/ml.
A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole co... more A general and sustainable multicomponent approach for the synthesis of benzimidazolyl-thiazole compounds via an efficient, one-pot, pseudo four-component reaction using 5-amino-2-mercaptobenzimidazole, aralkyl halides, ammonium thiocyanate, and substituted α-bromo-acetophenones in glacial acetic acid at ambient temperature to give final compounds (4a-p) in good yields in shorter time. The spectral data of synthesized compounds were evaluated by analytical and spectral techniques (IR, 1H-NMR, 13C-NMR, and ESI-HRMS). Further, some of the synthesized compounds were screened for their in-vitro antibacterial activity studies using the agar well diffusion method against Gram-positive Streptococcus Pneumoniae (2451) bacteria and Gram-negative Porteous Mirabilis (2081) bacteria. Based on the MIC results, it was observed that the most active compounds 4b, 4e, 4f, and 4k are shown promising anti-bacterial activity with the zone of inhibition values of 2.85 cm 2.75 cm, 3.6 cm, and 3.3 cm again...
Journal of Advanced Scientific Research
Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an econom... more Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an economical technique, which is employed as an efficient tool for microorganism identification and FTIR spectrum obtained for any identified compound gives the information on the unique fingerprint. During this study, an effort was made to interpret FTIR spectral data in differentiating the ability of antagonism of endophytic bacteria against Fusarium oxysporium var. lycopersii, a phytopathogen of tomato. Antagonism of chilli endophytic bacteria Pseudomonas aeruginosa AVSCE-1 (FR3) against Fusarium oxysporum- MTCC10270 (FO) were studied by dual culture method. FR3 showed significant antagonism against the growth of FO with inhibition of 62.5%. FTIR spectra of ethyl acetate extracts of FO, FR3 and FO+FR3 were analyzed. In FTIR analysis, C-O and C-C stretch of chitin at 1073.35 cm-1, C-N stretch of Amide II at 1232.97 cm-1, C-O stretch of Amide III at 1300 cm-1 and C=O stretch of lipid at 1650-18...
Int. J. of Aquatic Science, Jun 1, 2021
It turns out that there is a tremendous amount of wealth below the surface of the ocean waiting t... more It turns out that there is a tremendous amount of wealth below the surface of the ocean waiting to be discovered. The ocean environment is capable of solving many unsolved questions and is also home to an undiscovered hoard of mysteries. Seawater collected from Suryalanka marine sediments in Guntur, Andhra Pradesh, India was used to extract coastal soil samples. Selected bacterial strains were isolated using the spread plate technique, the bacteria with biological activity were screened using antibacterial activity, and the 16S rRNA sequencing was utilised to identify the bacteria. Metabolite which is biologically active was created in a defined setting (incubation, temperature, pH, Nacl concentration, and carbon amino acid and nitrogen sources). TLC was used to purify crude extract, which was then eluted with hexane to separate it on a column. Spectrally characterised purified fraction was examined for antibacterial and anticancer properties. Out of the 23 isolates, isolate AVSC3 exhibits a noticeable inhibitory activity against test organisms. This was established through a characterization of the test organism, Bacillus subtilis AVSC3, with a GenBank accession number of GEO:M21745. MN386243. To produce bioactive metabolites, an ideal incubation time is 60 hours, 35°C, pH 7, 0.5% NaCl, and 1% glucose and peptone. The antibacterial property of the pure fraction was found to be hexadecanoic acid, with a molecular formula of C16H32O2, which has spectral characteristics characteristic of this molecule. 14.2 mm-long Coli followed by Staphylococcus The 13.7-mm-diameter aureus, the 9-mm-diameter M.luteus, the 8.1-mm-diameter S.pyogenes, the 4.6-mm-diameter P. vulgaris, and the 4.4-mm-diameter S.typhi all demonstrated significant antimicrobial activity against MCF-7 cell lines, with the strongest effect observed at 25µg.
Int.J.Curr.Microbiol.App.Sci, 2017
L-asparaginase is used in the treatment of cancer, especially for acute lymphoblastic leukemia. B... more L-asparaginase is used in the treatment of cancer, especially for acute lymphoblastic leukemia. Bacterial species were isolated from the Nizampatnam mangrove sample and screened for its ability to produce the extracellular Lasparaginase enzyme. Effects of pH and temperature were investigated in the production of enzymes. The highest yield of the enzyme was observed when the pH of the fermentation cycle raised above 9 at 37°C. Effective production was observed while using Dextrose and Peptone as carbon and nitrogen source.
Recently there has been an explosion of information about novel bioactive compounds that have bee... more Recently there has been an explosion of information about novel bioactive compounds that have been isolated from marine microbes in an effort to further explore the relatively untapped marine microbes and their secondary metabolites for drug discovery. New therapeutic agents are urgently needed to treat medicinal needs that are currently unmet .The biodiversity of marine microbes and the versatility of their bioactive metabolites has not been fully explored. L-asparaginase therapy, alone is finding increased success in the management of acute lymphocytic leukemia's. Large amounts of purified enzyme and increasing recognition of successful therapy will generate increased demand. The production of L-asparaginase by using bacteria has attracted great attention owing to their cost effective and eco-friendly nature. In search of efficient microorganisms potential for production of anticancer enzymes, bacteria isolated from mangrove soil of Nizampatnam Guntur district were screened fo...
Arabian Journal of Chemistry, 2021
... 1) 2011 ISSN: 2231-2560 Research Article 659 Asian Journal of Biochemical and Pharmaceutical ... more ... 1) 2011 ISSN: 2231-2560 Research Article 659 Asian Journal of Biochemical and Pharmaceutical Research Clinical Manifestation & Novel Targets for HIV Therapy Niyaz Alam*, Alok Bhardwaj, Faisal Amin, Vivek Dabas, . Rameesh ...
Microorganisms are good sources for the production of biologically active secondary metabolites (... more Microorganisms are good sources for the production of biologically active secondary metabolites (Monaghan RL et al., 1990). Bacteria belonging to the genus Bacillus are among the most widely distributed microorganisms have produced antibiotics in the soluble protein structure and that these antibiotics have been found to be cheaper and more effective, these microorganisms are preferable for commercial production (Priest FG et al., 1989). Also, Strains of Bacillus are known producers of bioactive cyclic lipopeptides (Konz D et al., 1999). Bacillus has been investigated for their ability to produce so called bacteriocin like inhibitory substance BLIS. It has been reported that strains of B. thuringiensis, B. subtilis, B. stearothermophilus, B. licheniformis, B. megaterium and B. cereus produce BLIS (Motta AS et al., 2007). On the other hand, Bacillus subtilis showed a production of a large number of antibiotics, which are classified as non-ribosomal (Stein T et al., 2005). In another ...
World Journal of Dentistry
ORIGINAL RESEARCH agents include various antimicrobial agents, out of which mouth rinses are part... more ORIGINAL RESEARCH agents include various antimicrobial agents, out of which mouth rinses are particularly well accepted due to their ease of use and ability to reach places that are difficult to clean with a toothbrush. 6 Chlorhexidine mouth rinses (0.2%), among a myriad of oral hygiene products, have acquired an eponym of "gold standard in plaque control" owing to their remarkable therapeutic effect and substantivity. It is also accompanied by some disquieting characteristics such as extrinsic staining of teeth and tongue by attaching to the IntroductIon Oral health has a significant impact on general and oral health-related quality of life. Dental caries is one of the most prevalent, biofilm mediated, sugar-driven, and multifactorial dynamic diseases affecting most of the population, regardless of age, sex, or race. 1 Worldwide, approximately 36% of the population has dental caries in their permanent teeth, and in primary teeth, the incidence is about 9%. The overall prevalence rate of dental caries in children worldwide is 46.2% in primary teeth, whereas it is about 53.8% in permanent teeth. The reasons could be due to changes in dietary habits, illiteracy, improper oral hygiene practices, a dearth in the awareness on the significance of primary teeth, deficiency of conveyance facilities, and access to oral healthcare services. 2,3 Dental plaque is a major etiological factor in the initiation and progression of oral diseases such as dental caries and gingivitis. Plaque has a diverse microbial composition, out of which S. mutans is considered to be the primary contributing factor responsible for the initiation of dental caries. 4 Therefore, caries control programs include chemoprophylactic agents that target S. mutans. Plaque control can be accomplished either mechanically or chemically or by a combination of both. Mechanical plaque control includes tooth brushing and flossing. However, it may be limited by several factors, such as lack of time, poor manual dexterity skills, or deficient motivation. 5 Hence, a customary approach is to support mechanical plaque removal with a chemotherapeutic agent. Chemically available plaque control 1-4
Journal of microbiology, biotechnology and food sciences, 2019
Antiproliferative secondary metabolites producing bacterial strain AVSC4 isolated from marine sed... more Antiproliferative secondary metabolites producing bacterial strain AVSC4 isolated from marine sediments was identified as Bacillus flexus based on 16S rRNA gene sequence analysis. Under the strategy of liquid- liquid extraction, the crude extract was obtained showed significant antibacterial activity against different clinical pathogens. 0.5% methionine and 0.4% NaCl act as inducers for maximizing the growth and antibacterial activity of strain at pH 7 and 40°C. Heptadecanoic acid and methyl hexadecanoic acid were identified as major and dominant secondary metabolites by GC-MS analysis and also showed significant antiproliferative activity against HT-29 (Human colorectal adenocarcinoma) and A-549 (Lung Cancer) with IC50 value 93.4 µg/ml and 50.04 µg/ml.
The objective of the work was to isolate and screened for phosphate solubilization, multiple plan... more The objective of the work was to isolate and screened for phosphate solubilization, multiple plant growth promoting traits and phytopathogenic antagonism from chilli rhizosphere .The isolate putatively identified as Bacillus endophyticus AVP9 on the basis of biochemical characteristics and 16srRNA partial gene sequence analysis. Acid phosphatase activity of AVP9 was also characterized for the first time and its activity in correlation with growth was studied at different PH (3.0 to 4.8), 12 different amino acids and 15 carbon sources After growth optimization Bacillus endophyticus AVP9 showed 1112ppm inorganic phosphate solubilization and multiple plant growth promoting traits indole acetic acid(80µg), ammonia (110 µg) , HCN ,catalase and siderophore production . Maximum acid phosphatase activity was found at PH 3.4(0.2IU), Fructose (0.86IU) and Mannitol (0.83IU) and no correlation was observed with growth. Multiple potential of isolate AVP9 can be exploited in agricultural and indu...
To characterize and bioprocessing of oncolytic enzyme-L-asparaginase from novel marine bacteria b... more To characterize and bioprocessing of oncolytic enzyme-L-asparaginase from novel marine bacteria by statistical optimization for maximum asparaginase production. Out of 47, 9 bacterial isolates obtained from mangrove sediments showed positive Lasparaginase activity. One of the novel marine Bacillus AVP 14 was identified as Bacillus subtilis by 16S rRNA partial sequence and characterized for growth optimization and maximum production of anticancer enzyme. Solid state fermentation of L-Asparaginase production by using sesame oil cake, Groundnut oil cake, Red gram husk, Bengal gram husk, coconut oil cake, Green tea, Coffee was also done. Bacillus subtilis AVP 14 identified as potent extracellular producer of L-Asparaginase (347.42 IU) and showed 15 folds enhancement of L-asparaginase production (5,205 U/gds) under submerged fermentation condition in 5 days in presence of inducers and activators. The results emphasizes that submerged fermentation with Red gram yields more production than...