Bikash ranjan Jena | Acharya Nagarjuna University (original) (raw)

Papers by Bikash ranjan Jena

Current drug discovery technologies, Apr 29, 2024

Micro and nanosystems, Jan 25, 2024

Bioimpacts, Dec 31, 2022

Introduction: The mixed flavonoid supplement (MFS) [Trimethoxy Flavones (TMF) + epigallocatechin-... more Introduction: The mixed flavonoid supplement (MFS) [Trimethoxy Flavones (TMF) + epigallocatechin-3gallate (EGCG)] can be used to suppress inflammatory ulcers as an ethical medicine in Ayurveda. The inflammation of the rectum and anal regions is mostly attributed to nuclear factor kappa beta (NF-κB) signaling. NF-κB stimulates the expression of matrix metalloproteinase (MMP9), inflammatory cytokines tumor necrosis factor (TNF-α), and interleukin-1β (IL-1β). Although much research targeted the NF-κB and MMP9 signaling pathways, a subsequent investigation of target mediators in the inflammatory ulcer healing and NF-κB pathway has not been done. Methods: The docking studies of compounds TMF and EGCG were performed by applying PyRx and available software to understand ligand binding properties with the target proteins. The synergistic ulcer healing and anti-arthritic effects of MFS were elucidated using dextran sulfate sodium (DSS)-induced colon ulcer in Swiss albino rats. The colon mucosal injury was analyzed by colon ulcer index (CUI) and anorectic tissue microscopy. The IL-1β, tumor necrosis factor (TNF-α), and the pERK, MMP9, and NF-κB expressions in the colon tissue were determined by ELISA and Western blotting. RT-PCR determined the mRNA expression for inflammatory marker enzymes. Results: The docking studies revealed that EGCG and TMF had a good binding affinity with MMP9 (i.e.,-6.8 and-6.0 Kcal/mol) and NF-kB (-9.4 and 8.3 kcal/mol). The high dose MFS better suppressed ulcerative colitis (UC) and associated arthritis with marked low-density pERK, MMP9, and NF-κB proteins. The CUI score and inflammatory mediator levels were suppressed with endogenous antioxidant levels in MFS treated rats. Conclusion: The MFS effectively unraveled anorectic tissue inflammation and associated arthritis by suppressing NF-κB-mediated MMP9 and cytokines.

Bikash Ranjan Jena has over 8.5 years of academic and research experience in the field of Pharmac... more Bikash Ranjan Jena has over 8.5 years of academic and research experience in the field of Pharmaceutical Analysis, especially systematic development and validation of some selected anticancer molecules employing Analytical Quality by Design (AQbD) approach and Design of Experiments (DoE). Currently, he is perusing his Ph.D.

International Journal of Drug Research and Technology, Jun 30, 2017

The present study demonstrates a simple, precise and accurate UV spectrophotometric method develo... more The present study demonstrates a simple, precise and accurate UV spectrophotometric method development for the quantitative estimation of glipizide in bulk and pharmaceutical dosage forms. The analysis was carried out by using Elico SL 159 UV-Visible spectrophotometer with 1cm matched quartz cells. In this study, the zero order spectrum of glipizide in the presence of phosphate buffer (pH 3.8) is conducted and measured the absorbance at λ max 230 nm. The method was verified by using various validation parameters such as linearity, accuracy, precision. The calibration curve was linear over the concentration range tested (1-50 μg/mL). The accuracy and precision studies were carried out which is less than 2% that indicates good accuracy and precision values. There is no interference of excipients used in the formulation as low % RSD values in the recovery studies. These results indicate that the method shows a practical application as a quality control tool for analysis of the drug in its tablet dosage forms in pharmaceutical industries. The developed method was validated according to ICH-Q1C guidelines and it is applicable for the analysis of bulk drug in its tablet dosage forms.

BioImpacts

Journal of Ethnopharmacology, 2019

Ethnopharmacological relevance: The plant Tabebuia chrysantha (Jaq.) Nicholson (Bignoniaceae) is ... more Ethnopharmacological relevance: The plant Tabebuia chrysantha (Jaq.) Nicholson (Bignoniaceae) is commonly known as "Golden Goddess" in the Southern part of India and "Golden Trumpet Tree " in Central America. Stems of this plant have been traditionally used for antioxidant, anti-inflammatory, antimicrobial and anticancer actions. Aim of the study: To evaluate the antitumor activity of methanol extract of Tabebuia chrysantha stem (METC). Materials and methods: The in vivo antitumor potential of METC against Ehrlich Ascites Carcinoma (EAC) in Swiss albino mice was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters, biochemical parameters, and antioxidant parameters. The in vitro antitumor potential of METC at different concentrations (100, 200, 400, 800, 1000) µg/mL has been evaluated, by using the MTT [3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and Trypan blue dilution assay for a period of 3 h treatment. Before that, the crude extract was pre-screened for cytotoxicity property using Brine shrimp lethality bioassay. Results: Phytoconstituents 2-Hydroxynaphthalene-1,4-dione, β-lapachone and 2-((dimethylamino)methyl)-3methoxynaphthalene-1,4-dione were isolated from the METC. Their occurrence and structures were determined by HPLC chromatography, UV spectroscopy, and 1D and 2D NMR spectroscopies respectively. The extract showed a direct cytotoxic effect on EAC cells in a dose-dependent manner with IG 50 value 463.27 µg/mL in MTT assay and 443.58 µg/mL in trypan blue dilution assay. The METC (300 mg/kg) and 5-FU (30 mg/kg) exhibited significant (p < 0.001) decrease in tumor volume, tumor weight and viable cell count of EAC-treated mice. Also, hematological profile, tissue parameters such as lipid peroxidation, reduced glutathione, superoxide dismutase, and catalase were reverted to the normal levels compared to the EAC control group. The Western blotting analysis demonstrated apoptosis of carcinoma was due to inhibition of soluble epidermal growth factor receptor proteins (sEGFR) expression in the blood. Conclusion: The antitumor potential of the stem extract of T chrysantha was due to naphthaquinones and polyphenol content in the crude extract and so T chrysantha could be a cytotoxic plant to control tumor growth.

Current Pharmaceutical Biotechnology

: The current review intends to regulate and accurately evaluate genotoxic contaminants in drug s... more : The current review intends to regulate and accurately evaluate genotoxic contaminants in drug substance and drug product method and formulation process development, validation, and degradation pathways. The quality by design (QbD) principles can be applied to the systematic evaluation and control of impurities enabled by the development of modern analytical techniques, including the performance of risk assessment, the screening of critical process parameters (CPPs), and the identification of the most influential variables in the optimization of the evaluation and control methods. Current difficulties in removing genotoxic contaminants and the procedures for doing so have been outlined in this review, along with the steps necessary to acquire optimum techniques and the most acceptable formulations. In addition to this, division, characterization, assessment, quantification, and formation of genotoxic impurities sources and control strategy for genotoxic impurities, handling of nitrosamine assay content of drug products in different industrial methodologies and their chemometric prospects and associated recent patents are also explored.

Current Analytical Chemistry

Introduction: The objective of this study is to provide a rapid, sensitive, consistent, and coste... more Introduction: The objective of this study is to provide a rapid, sensitive, consistent, and costeffective method for quantifying isradipine using ultra-fast liquid chromatography. Methods: Quality by Design principles will form the basis of this approach, grounded on response surface analysis. Shimadzu liquid chromatographic system equipped with a photodiode array detector and LC solution software was used to conduct the RP-UFLC method development and validation. An ODS C18 (250 x 4.6 mm; 5 μm) UFLC column was used to complete the analysis. The RSM methodology utilized a central composite design to perform the optimization studies. Results: The mobile phase ratio and flow rate were considered crucial method parameters, as well as the peak area, retention time, and USP plate were considered critical analytical attributes. The optimal conditions for chromatographic separation were followed using 80% acetonitrile and water (20% v/v) as mobile phase, a 1 mL/min flow rate, an injection v...

Nanoscience & Nanotechnology-Asia

: In the last few years, nanozymes have emerged as an adequate substitute for natural enzymes. Re... more : In the last few years, nanozymes have emerged as an adequate substitute for natural enzymes. Recently, much attention has been paid to enzyme-mimic nanomaterials (nanozymes). Because of their distinct characteristics, they are a critical alternative to natural enzymes that can be produced at a subordinate cost and more efficiently. These nanomaterials have enzyme-like activity and have been cast off to detect and treat biomolecules such as DNA, proteins, cells, and tiny molecules such as glucose. Hence, the critical analysis of recent nanozyme is deemed essential for futuristic research, outcome-based results specified to current trends of analytical tools, and several disease monitoring for targeted oncology therapies like circulating tumor cells, MRI, PET, etc. In addition, the multivariate applications of nanozymes for biosensors, immunoassay formation, tumor cell detection with earlier remedies, and environmentally-sound engineering technologies are discussed to climax the modern advancements. The novelty and originality of this current review are to intensify the recent advancement, biomedical applications, types and mimicking activity, biomedical applications of nanozymes, implementation of the chemometric approach in nanozymes, and its futuristic approach. Finally, to promote the understanding of nanozymes and the development of novel and multifunctional nanozymes, we provide a comprehensive review of the nanozymes with their broadest applications and modern technologies involved in targeted drug discovery inventory with other diversified arenas and existing patents indicating future implications.

Purpose: To increase the drug's oral bioavailability, a self-nano emulsifying drug delivery s... more Purpose: To increase the drug's oral bioavailability, a self-nano emulsifying drug delivery system was designed using capmul MCM, labrasol, and tween-20 as the oil, surfactant, and co-surfactant, respectively. Oil and Smix were tested for pre-isotropic compatibility and optimization of the formulations by using DoE software. Dispersibility, self-emulsifying duration, mean globule size, and stability were determined by a heating-cooling cycle and phase separation. Methods: Self-nano emulsifying systems were created into free-flowing granules by adsorbing using the ratios of aerosil 200, sylysia 350, 550, and 730 as porous carriers and neusilin as an adsorbing agent. The FT-IR study assessed the drug's compatibility with various excipients and confirm no interaction. The produced granules were analyzed using differential scanning calorimetry, dissolution profile, and other flow property measures. The SEM examination revealed no evidence of drug precipitation on the carrier&#39...

Acta Scientific Pharmaceutical Sciences

Drug Delivery Letters

Background: The prime intent of this study was to formulate, optimize and evaluate the floating m... more Background: The prime intent of this study was to formulate, optimize and evaluate the floating microballoons of rosuvastatin calcium to extend the stomach or gastrointestinal residence time, dissolution rate, and bioavailability of the drug. Objective: Objective: Rosuvastatin calcium-loaded floating microballoons were prepared by solvent evaporation technique and systematic optimization of such formulations by response surface methodology using Box-Behnken Design, with the selected independent variables like concentration of HPMC K4M (X1), K15M (X2), and K100M (X3) and dependent variables as mean particle size in µm (R1), % entrapment efficiency (R2), and % drug released at 12h (R3). Methods: For each of the studied response variables, the trial formulations were subsequently evaluated for in vitro floating lag time, drug content, total floating time, and drug content, and the data analysis through optimization was carried out by placing the experimental data with an appropriate ma...

Beni-Suef University Journal of Basic and Applied Sciences

Background The current study expands on the use of design of experiment in developing cefuroxime ... more Background The current study expands on the use of design of experiment in developing cefuroxime axetil mucoadhesive minitablets for treating antibiotic-associated colitis. A comprehensive QbD-based product development strategy was implemented, with the target product profile defined based on the desired product quality of mucoadhesive minitablets. The identified critical quality attributes are based on the target product profile. The goal was to find the optimum levels by using the concentrations of chitosan (mg) (X1), HPMC K100M (X2) and sodium carboxymethyl cellulose (X3) as the influential variables. The response surface methodology determines the dependent variables using 33 Box–Behnken design to optimize the selected critical factors. The friability (%), drug content (%) and mucoadhesive strength (%) characteristics of cefuroxime axetil mucoadhesive minitablets were evaluated using ANOVA for the observed responses or dependent variables. Results The study demonstrated that run...

Current Pharmaceutical Biotechnology, Mar 29, 2022

: Platelet-inspired nanoparticles have ignited the possibility of new opportunities for producing... more : Platelet-inspired nanoparticles have ignited the possibility of new opportunities for producing similar biological particulates, such as structural cellular and vesicular components, as well as various viral forms, to improve biocompatible features that could improve the nature of biocompatible elements and enhance therapeutic efficacy. The simplicity and more effortless adaptability of such biomimetic techniques uplift the delivery of the carriers laden with cellular structures, which has created varied opportunities and scope of merits like; prolongation in circulation and alleviating immunogenicity improvement of the site-specific active targeting. Platelet-inspired nanoparticles or medicines are the most recent nanotechnology-based drug targeting systems used mainly to treat blood-related disorders, tumors, and cancer. The present review encompasses the current approach of platelet-inspired nanoparticles or medicines that have boosted the scientific community from versatile fields to advance biomedical sciences. Surprisingly, this knowledge has streamlined to development of newer diagnostic methods, imaging techniques, and novel nanocarriers, which might further help in the treatment protocol of the various diseased conditions. The review primarily focuses on the novel advancements and recent patents in nanoscience and nanomedicine that could be streamlined in the future for the management of progressive cancers and tumor targeting. Rigorous technological advancements like biomimetic stem cells, pH-sensitive drug delivery of nanoparticles, DNA origami devices, virosomes, nano cells like exosomes mimicking nanovesicles, DNA nanorobots, microbots, etc., can be implemented effectively for target-specific drug delivery.

Nanoscience &Nanotechnology-Asia, 2019

Background: Irbesartan is an anti-hypertensive BCS class II drug exhibiting poor aqueous solubili... more Background: Irbesartan is an anti-hypertensive BCS class II drug exhibiting poor aqueous solubility, which makes it highly challenging for delivery through the oral route. Based on this fact, a self-microemulsifying drug delivery system (SMEDDS) was designed and characterized for augmenting the aqueous solubility and dissolution rate of irbesartan. Methods: Several blends of oil (Capmul MCM EP), surfactant (Tween 80) and co-surfactant (PEG 600) were screened from the preliminary solubility and pseudo-ternary phase diagram studies. Systematic optimization of the SMEDDS was carried out using 3-factor 3-level Box-Behnken design. Results: The optimized formulation was identified by numerical optimization technique, which revealed faster emulsification time, high percent transmittance and drug content, lower globule size < 100 nm, zeta potential and excellent thermodynamic stability. The optimal formulation unveiled more than 93.3% drug release in vitro within 60 minutes, while the pu...