Shan Taylor | Edinburgh Napier University (original) (raw)
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Papers by Shan Taylor
Brain Research, 2006
This report describes the identification and characterization of the murine orphan GPCR, Gpr101. ... more This report describes the identification and characterization of the murine orphan GPCR, Gpr101. Both human and murine genes were localized to chromosome X. Similar to its human ortholog, murine Gpr101 mRNA was detected predominantly in the brain within discrete nuclei. A knowledge-restricted hidden Markov model-based algorithm, capable of accurately predicting G-protein coupling selectivity, indicated that both human and murine GPR101 were likely coupled to Gs. This prediction was supported by the elevation of cyclic AMP levels and the activation of a cyclic AMP response element-luciferase reporter gene in HEK293 cells over-expressing human GPR101. Consistent with this, over-expression of human GPR101 in a yeast-based system yielded an elevated, agonist-independent reporter gene response in the presence of a yeast chimeric Gαs protein. These results indicate that GPR101 participates in a potentially wide range of activities in the CNS via modulation of cAMP levels. ava i l a b l e a t w w w. s c i e n c e d i r e c t . c o m w w w. e l s ev i e r. c o m / l o c a t e / b r a i n r e s
This paper is dedicated to the memory of our ATLAS colleagues who did not live to see the full im... more This paper is dedicated to the memory of our ATLAS colleagues who did not live to see the full impact and significance of their contributions to the experiment.
Preventive Medicine, 2011
Brain Research, 2006
This report describes the identification and characterization of the murine orphan GPCR, Gpr101. ... more This report describes the identification and characterization of the murine orphan GPCR, Gpr101. Both human and murine genes were localized to chromosome X. Similar to its human ortholog, murine Gpr101 mRNA was detected predominantly in the brain within discrete nuclei. A knowledge-restricted hidden Markov model-based algorithm, capable of accurately predicting G-protein coupling selectivity, indicated that both human and murine GPR101 were likely coupled to Gs. This prediction was supported by the elevation of cyclic AMP levels and the activation of a cyclic AMP response element-luciferase reporter gene in HEK293 cells over-expressing human GPR101. Consistent with this, over-expression of human GPR101 in a yeast-based system yielded an elevated, agonist-independent reporter gene response in the presence of a yeast chimeric Gαs protein. These results indicate that GPR101 participates in a potentially wide range of activities in the CNS via modulation of cAMP levels. ava i l a b l e a t w w w. s c i e n c e d i r e c t . c o m w w w. e l s ev i e r. c o m / l o c a t e / b r a i n r e s
This paper is dedicated to the memory of our ATLAS colleagues who did not live to see the full im... more This paper is dedicated to the memory of our ATLAS colleagues who did not live to see the full impact and significance of their contributions to the experiment.
Preventive Medicine, 2011