Dr. Sharangouda J. Patil | Indian Council of Agricultural Research (ICAR) (original) (raw)
Papers by Dr. Sharangouda J. Patil
International journal of pharmaceutical quality assurance, Jun 25, 2024
The unregulated spread of abnormal cells across the body characterizes cancer, one of the most de... more The unregulated spread of abnormal cells across the body characterizes cancer, one of the most devastating diseases that humanity has ever encountered. The normal process of cell division occurs when the body needs new cells to replace damaged or dead cells. The study found that Nigella sativa stem has anticancer activity, with phenols, flavonoids, saponins, and glycosides present in its phytochemical screening. In an in-vivo study, rats supplemented with N. sativa showed no significant changes in liver and heart architecture, serum ALT and AST levels, or liver tissue pathology. We still don't know which chemical components in the stem are responsible for its anticancer action, but the study did find that participants' body weights stayed healthy.
With more and more advances in the field of electrophysiological studies and techniques, with imp... more With more and more advances in the field of electrophysiological studies and techniques, with improvised and advanced neuroimaging techniques, the diagnostic accuracy has increased over the years. Although satisfactory treatment of this condition is still to come, pharmacotheurapcitics and drug trails are ongoing for the search of novel drugs for better control of symptoms. In the last few decades, surgery either external or percutanous procedures has provided a number of treatment options in terms of cost effectiveness, minimal invasiveness and morbidity. Maxillary and mandibular divisions were more frequently involved with higher incidence of left side involvement. 45.1% reported definite triggering factor/factors with tactile stimulus and cold face wash being the common triggering factors. 83.8% complained symptoms as electric shock like sensation followed by intense stabbing / pricking and burning pain in 70.9% and 67.0% respectively. Latencies of blink reflex were absent or prolonged in 41.9% of patients. 80% of patients with abnormal blink reflex had abnormalities in MRI too which was statistically significant (P=0.007). Severity of pain did not correlate with either MRI or EPS (P>0.05)
Social Science Research Network, Nov 21, 2019
Social Science Research Network, Nov 25, 2020
Social Science Research Network, Dec 31, 2020
International research journal of pharmacy, 2018
Human Herpesvirus 3 (HHV3), commonly known as the Varicella zoster virus (VZV), is a highly infec... more Human Herpesvirus 3 (HHV3), commonly known as the Varicella zoster virus (VZV), is a highly infectious α-herpesvirus that causes Varicella (chicken pox) on primary infection, as well as Herpes zoster (shingles) on latent reactivation. Lifethreatening sequelae and complications such as post-herpetic neuralgia (PHN), meningoencephalitis, myelopathy, skin necrosis, etc. have also been observed in some immunocompromised individuals. Although effective vaccines have been developed against VZV, they are limited by the scope of vaccination and in some cases by the vaccine e cacy. Addressing the need for a much safer and more e cient vaccine, an in-silico approach-based study was carried out to design a multi-epitope vaccine against VZV. Accounting for their crucial role in the viral entry mechanism, the envelope glycoproteins were analyzed for potent epitopes that can induce cytotoxic T cells, Helper T cells, and B cells. These epitopes were further screened using various computational tools, for constructing a multi-epitope vaccine, via the addition of suitable linkers and an adjuvant. Antigenicity, allergenicity, conservancy, population coverage, and other physiochemical properties of the nal vaccine construct were evaluated, post which their structural properties and the interaction patterns with TLR1 and TLR4 were analyzed. Further, the vaccine construct was subjected to molecular dynamics simulation studies, in order to assess its structural stability. Finally, the multi-epitope vaccine was codon optimized and cloned into the expression vector pET-28a+, to have it expressed e ciently in the K12 strain of E.Coli bacteria. The immune reactions triggered by the vaccine candidate were validated using immune simulation studies. Laboratory validation of the ndings for its immunogenicity and safety can help in assessing the in vivo e ciency of the vaccine to prevent VZV infection.
Silk is a globally renowned abundant biopolymer obtained from various sources of the Lepidoptera ... more Silk is a globally renowned abundant biopolymer obtained from various sources of the Lepidoptera family, among which the most commonly used and researched are spider silk and silk worm silk. All varieties of silk have beneficial characteristics such as high tensile strength, biocompatibility, producing a reduced immune response in a biological system, biodegradability, and the ability to withstand environmental stresses as well. These features make silk suitable for a number of applications as a biomaterial. The vast potential of silk and its proteins in cosmetics, oncology, tissue engineering, TOC screenings, for preserving food, cosmetic product as a silk gel and bioremediation makes it a well-sought biopolymer among researchers. Experiments over the years have revealed that biomaterials constituting silk are very potent but are yet to be scaled up for commercial uses, but the various advantageous properties of silk biomaterial far overshadows the impeding problems of production.
Asian Journal of Pharmaceutical Analysis
Nucleic acid identification, separation, purification, sequencing, diagnosis mapping, etc. are em... more Nucleic acid identification, separation, purification, sequencing, diagnosis mapping, etc. are emerging field of bioscience. While designing acceptable method is crucial part of analyser. The repetitive task assigned during method the development and controlling the internal as well as external parameters may design a prominent approach for identification. The interpretation is also directed towards functional complexity of genetic disorders. The high-resolution techniques are minimize the biological barriers in recent developments and focusing on independent structure. The nucleic acid analysis is increasing in the field of bioscience and most preferable application in diagnostic laboratories at commercial scale. Identification of nucleic acid sequence will going to be applied in the analytical aspects like forensic samples, medicines, diseases identification, staging and few more environmental applications. The paper highlights the recent developments of laboratory methods in nucl...
Environmental Sciences
The present study reveals the correlation of zooplankton productivity of lotic and lentic water e... more The present study reveals the correlation of zooplankton productivity of lotic and lentic water ecosystem. The biological rhythms are useful tool to determine the zooplankton production. Planktons enrich the trophic level of lentic and lotic ecosystems. The relationship of primary productivity of any aquatic ecosystem depends on the biological biodiversity. Estimation of zooloplankton productivity of any water system helps to analyse its richness of species as biological population which are sustainable in it by the adequate amount physicochemical parameters. It is observed in most of the water system the quality of physical, chemical and biological phenomena are depends directly or indirectly correlation to establish diverse life as suitable habitat. To identify the current problems on lentic and lotic water system correlation studies will be more helpful, it is need of hour to give more attention on limnology because most of the biodiversity polluted due to industrialisation and a...
Social Science Research Network, Feb 13, 2020
Research Journal of Medicinal Plant, 2019
Social Science Research Network, Dec 1, 2020
Indian Journal of Pharmaceutical Sciences, 2010
12. Agarwala SC, Sharma CP. The standardization of sand culture technique for the study of macro ... more 12. Agarwala SC, Sharma CP. The standardization of sand culture technique for the study of macro and micro (trace) element deficiencies under Indian conditions. Curr Sci 1961;11:427. 13. Srivastava NK, Luthra R. Distribution of Photo-synthetically fixed 14 CO 2 into essential oil in relation to primary metabolites in developing peppermint (Mentha piperita) leaves.
Journal of Advanced Medical Sciences and Applied Technologies, 2017
In India control of fertility is based on the folk use of numerous Indian Traditional Plants that... more In India control of fertility is based on the folk use of numerous Indian Traditional Plants that has been practiced for contraception from many years. Present investigation aims that, to evaluate the contraceptive potential of Achyranthes aspera leaves MeOH extract in male albino mice and has shown promising results of antispermatogenic, antiandrogenic and hypolipidaemic activities. Materials & Methods: MeOH extract of A. aspera leaves at the doses of 25 mg and 50 mg/100 gm body weight was administered orally for 30 days to adult male albino mice. On day 31st, the rats were sacrificed and the testis were removed and weighed for gravimetrical analysis. Organs were processed for biochemical estimation, histological evaluation and histomorphometry for testicular analysis. Results: Treatment with A. aspera leaves extract resulted in decrease the weight of testis 0.925±0.015 significantly (P<0.05). Morphometric analysis like diameter of testis 2554.22±18.545 seminiferous tubules 247.074±8.051 and Leydig cells 7.842±0.248 were decreased significantly (P<0.05) at 50mg/100gm body weight when compare to control. Spermatogenic elements like, spermatogonia, spermatocytes and spermatids in the testis were reduced significantly (P<0.05) as well as Leydig cells count in testis when compared to control. Biochemical analysis resulted as, protein, glycogen, and cholesterol content were decreased significantly (P<0.05) in testis of extracts treated mice, whereas alkaline phosphatase increased and acid phosphatase decreased when compared with that of control. Conclusion: MeOH extract of A. aspera leaves arrest the spermatogenesis and inhibited testicular function in male mice without side effects to act as potential contraceptive.
Pharmaceutical Chemistry Journal
Asian Journal of Biological and Life sciences
The present study deals with the isolation of Rhizobium bacteria from root nodules of Mimosa pudi... more The present study deals with the isolation of Rhizobium bacteria from root nodules of Mimosa pudica, and to check its efficiency as bio-fertilizer in improving plant growth. Rhizobium sps SN01 was isolated from root nodules of Mimosa pudica and confirmed to be Rhizobium bacteria through following biochemical tests of indole, methyl red, Voges-Proskauer and citrate utilization (IMViC), urease, hydrogen sulphide, carbohydrate fermentation tests. Rhizobium sps SN01 was analysed for antibiotic sensitivity test towards three different antibiotics such as Azithromycin, Amoxicillin and Cephalosporin. To check the efficiency, seeds of legume crops like cow peas, peas, and fenugreek were treated with Rhizobium sps, SN01, sowed in soil and coco-peat to study its germination rate and effectiveness in plant growth resulting in root length, shoot length, protein content and chlorophyll content. Impact of Rhizobium sps SN01 bacteria in soil and coco-peat quality and improvement was also focused by analyzing micro and macro nutrients responsible for its quality to improve plant growth.
Advances in Pharmacology and Pharmacy, 2021
Traditional Indian medicinal plant Simarouba glauca is a highly sacred plant known as "Paradise T... more Traditional Indian medicinal plant Simarouba glauca is a highly sacred plant known as "Paradise Tree or Lakshmi Taru" which is recognized for its pharmacological and pharmaceutical properties. The present study was envisaged to know the in vitro phytochemical, antioxidant and free radical scavenging activity of S. glauca seeds. Petroleum ether was used as a solvent to extract plant material using hot extraction method. In our investigation, phytochemical analysis was carried out qualitatively and quantitatively and for the evaluation of antioxidant profile carried out in vitro studies on free radical scavenging potential by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, decolourization potential of 2,2'-azino-bis[3-ethyl benzthiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant potential (FRAP) assay were determined in in vitro studies. The qualitative phytochemical analysis of this plant was positive for flavonoids, fatty acids, proteins, steroids and terpenoids and in quantitative analysis total flavonoid content was 17.32±0.12 mg/μg quercetin equivalent, total proanthocyanidin content exhibited 62.91±0.61 µg catechin equivalent, total phenol content exhibited 17.75±5.82 μg gallic acid equivalent and the total flavonol content was 1.54±0.01 µg quercetin equivalent of the extract. In the antioxidant profile, the maximum DPPH scavenging activity exhibited was 74% with an IC 50 value 137.89 µg/mL, the maximum ABTS scavenging activity exhibited was 35% with an IC 50 value 337.29 µg/mL and FRAP scavenging activity exhibited 62.5 µg/mL as ascorbic acid equivalents (AAE/ml) concentration. These findings evidenced that, the petroleum ether extract of S. glauca seeds has potential source of natural antioxidants and can be used it as therapeutic medicine.
International Journal of Current Pharmaceutical Research, 2016
Objective: Karnataka (India) is the darling child of nature where about 722 medicinal plants are ... more Objective: Karnataka (India) is the darling child of nature where about 722 medicinal plants are available. Ions of different metal elements have an important role in both the reactions. Few elements are essential to the body as nutrients called minerals. Present study deals with trace element analysis was carried out in Ballari district, Karnataka, India.Methods: 0.25g each of the powdered medicinal plant spice seeds samples digested in 6.5 ml of acid solution to study the trace element like Mn, Fe, Cu, Zn, Cd, and Cr in Coriander seeds, Ajwain seeds, Pepper seeds, Cumin seeds and Sweet cumin seeds at ppm levels were figured out by using AAS analysis.Results: Analysed trace mineral contents of these spice plants resulted highest level of Manganese is 1.92 ppm, Ferrous is 3.69 ppm and in Chromium is 0.0980 ppm in Ajwain seeds, Copper is 0.42 ppm in Coriander seeds, Zinc is 3.02 ppm and Cadmium is 0.0070 ppm in Sweet cumin seeds and lowest were found Mn is 0.65 ppm, Fe is 2.43 ppm in...
Springer International Publishing eBooks, 2022
International journal of pharmaceutical quality assurance, Jun 25, 2024
The unregulated spread of abnormal cells across the body characterizes cancer, one of the most de... more The unregulated spread of abnormal cells across the body characterizes cancer, one of the most devastating diseases that humanity has ever encountered. The normal process of cell division occurs when the body needs new cells to replace damaged or dead cells. The study found that Nigella sativa stem has anticancer activity, with phenols, flavonoids, saponins, and glycosides present in its phytochemical screening. In an in-vivo study, rats supplemented with N. sativa showed no significant changes in liver and heart architecture, serum ALT and AST levels, or liver tissue pathology. We still don't know which chemical components in the stem are responsible for its anticancer action, but the study did find that participants' body weights stayed healthy.
With more and more advances in the field of electrophysiological studies and techniques, with imp... more With more and more advances in the field of electrophysiological studies and techniques, with improvised and advanced neuroimaging techniques, the diagnostic accuracy has increased over the years. Although satisfactory treatment of this condition is still to come, pharmacotheurapcitics and drug trails are ongoing for the search of novel drugs for better control of symptoms. In the last few decades, surgery either external or percutanous procedures has provided a number of treatment options in terms of cost effectiveness, minimal invasiveness and morbidity. Maxillary and mandibular divisions were more frequently involved with higher incidence of left side involvement. 45.1% reported definite triggering factor/factors with tactile stimulus and cold face wash being the common triggering factors. 83.8% complained symptoms as electric shock like sensation followed by intense stabbing / pricking and burning pain in 70.9% and 67.0% respectively. Latencies of blink reflex were absent or prolonged in 41.9% of patients. 80% of patients with abnormal blink reflex had abnormalities in MRI too which was statistically significant (P=0.007). Severity of pain did not correlate with either MRI or EPS (P>0.05)
Social Science Research Network, Nov 21, 2019
Social Science Research Network, Nov 25, 2020
Social Science Research Network, Dec 31, 2020
International research journal of pharmacy, 2018
Human Herpesvirus 3 (HHV3), commonly known as the Varicella zoster virus (VZV), is a highly infec... more Human Herpesvirus 3 (HHV3), commonly known as the Varicella zoster virus (VZV), is a highly infectious α-herpesvirus that causes Varicella (chicken pox) on primary infection, as well as Herpes zoster (shingles) on latent reactivation. Lifethreatening sequelae and complications such as post-herpetic neuralgia (PHN), meningoencephalitis, myelopathy, skin necrosis, etc. have also been observed in some immunocompromised individuals. Although effective vaccines have been developed against VZV, they are limited by the scope of vaccination and in some cases by the vaccine e cacy. Addressing the need for a much safer and more e cient vaccine, an in-silico approach-based study was carried out to design a multi-epitope vaccine against VZV. Accounting for their crucial role in the viral entry mechanism, the envelope glycoproteins were analyzed for potent epitopes that can induce cytotoxic T cells, Helper T cells, and B cells. These epitopes were further screened using various computational tools, for constructing a multi-epitope vaccine, via the addition of suitable linkers and an adjuvant. Antigenicity, allergenicity, conservancy, population coverage, and other physiochemical properties of the nal vaccine construct were evaluated, post which their structural properties and the interaction patterns with TLR1 and TLR4 were analyzed. Further, the vaccine construct was subjected to molecular dynamics simulation studies, in order to assess its structural stability. Finally, the multi-epitope vaccine was codon optimized and cloned into the expression vector pET-28a+, to have it expressed e ciently in the K12 strain of E.Coli bacteria. The immune reactions triggered by the vaccine candidate were validated using immune simulation studies. Laboratory validation of the ndings for its immunogenicity and safety can help in assessing the in vivo e ciency of the vaccine to prevent VZV infection.
Silk is a globally renowned abundant biopolymer obtained from various sources of the Lepidoptera ... more Silk is a globally renowned abundant biopolymer obtained from various sources of the Lepidoptera family, among which the most commonly used and researched are spider silk and silk worm silk. All varieties of silk have beneficial characteristics such as high tensile strength, biocompatibility, producing a reduced immune response in a biological system, biodegradability, and the ability to withstand environmental stresses as well. These features make silk suitable for a number of applications as a biomaterial. The vast potential of silk and its proteins in cosmetics, oncology, tissue engineering, TOC screenings, for preserving food, cosmetic product as a silk gel and bioremediation makes it a well-sought biopolymer among researchers. Experiments over the years have revealed that biomaterials constituting silk are very potent but are yet to be scaled up for commercial uses, but the various advantageous properties of silk biomaterial far overshadows the impeding problems of production.
Asian Journal of Pharmaceutical Analysis
Nucleic acid identification, separation, purification, sequencing, diagnosis mapping, etc. are em... more Nucleic acid identification, separation, purification, sequencing, diagnosis mapping, etc. are emerging field of bioscience. While designing acceptable method is crucial part of analyser. The repetitive task assigned during method the development and controlling the internal as well as external parameters may design a prominent approach for identification. The interpretation is also directed towards functional complexity of genetic disorders. The high-resolution techniques are minimize the biological barriers in recent developments and focusing on independent structure. The nucleic acid analysis is increasing in the field of bioscience and most preferable application in diagnostic laboratories at commercial scale. Identification of nucleic acid sequence will going to be applied in the analytical aspects like forensic samples, medicines, diseases identification, staging and few more environmental applications. The paper highlights the recent developments of laboratory methods in nucl...
Environmental Sciences
The present study reveals the correlation of zooplankton productivity of lotic and lentic water e... more The present study reveals the correlation of zooplankton productivity of lotic and lentic water ecosystem. The biological rhythms are useful tool to determine the zooplankton production. Planktons enrich the trophic level of lentic and lotic ecosystems. The relationship of primary productivity of any aquatic ecosystem depends on the biological biodiversity. Estimation of zooloplankton productivity of any water system helps to analyse its richness of species as biological population which are sustainable in it by the adequate amount physicochemical parameters. It is observed in most of the water system the quality of physical, chemical and biological phenomena are depends directly or indirectly correlation to establish diverse life as suitable habitat. To identify the current problems on lentic and lotic water system correlation studies will be more helpful, it is need of hour to give more attention on limnology because most of the biodiversity polluted due to industrialisation and a...
Social Science Research Network, Feb 13, 2020
Research Journal of Medicinal Plant, 2019
Social Science Research Network, Dec 1, 2020
Indian Journal of Pharmaceutical Sciences, 2010
12. Agarwala SC, Sharma CP. The standardization of sand culture technique for the study of macro ... more 12. Agarwala SC, Sharma CP. The standardization of sand culture technique for the study of macro and micro (trace) element deficiencies under Indian conditions. Curr Sci 1961;11:427. 13. Srivastava NK, Luthra R. Distribution of Photo-synthetically fixed 14 CO 2 into essential oil in relation to primary metabolites in developing peppermint (Mentha piperita) leaves.
Journal of Advanced Medical Sciences and Applied Technologies, 2017
In India control of fertility is based on the folk use of numerous Indian Traditional Plants that... more In India control of fertility is based on the folk use of numerous Indian Traditional Plants that has been practiced for contraception from many years. Present investigation aims that, to evaluate the contraceptive potential of Achyranthes aspera leaves MeOH extract in male albino mice and has shown promising results of antispermatogenic, antiandrogenic and hypolipidaemic activities. Materials & Methods: MeOH extract of A. aspera leaves at the doses of 25 mg and 50 mg/100 gm body weight was administered orally for 30 days to adult male albino mice. On day 31st, the rats were sacrificed and the testis were removed and weighed for gravimetrical analysis. Organs were processed for biochemical estimation, histological evaluation and histomorphometry for testicular analysis. Results: Treatment with A. aspera leaves extract resulted in decrease the weight of testis 0.925±0.015 significantly (P<0.05). Morphometric analysis like diameter of testis 2554.22±18.545 seminiferous tubules 247.074±8.051 and Leydig cells 7.842±0.248 were decreased significantly (P<0.05) at 50mg/100gm body weight when compare to control. Spermatogenic elements like, spermatogonia, spermatocytes and spermatids in the testis were reduced significantly (P<0.05) as well as Leydig cells count in testis when compared to control. Biochemical analysis resulted as, protein, glycogen, and cholesterol content were decreased significantly (P<0.05) in testis of extracts treated mice, whereas alkaline phosphatase increased and acid phosphatase decreased when compared with that of control. Conclusion: MeOH extract of A. aspera leaves arrest the spermatogenesis and inhibited testicular function in male mice without side effects to act as potential contraceptive.
Pharmaceutical Chemistry Journal
Asian Journal of Biological and Life sciences
The present study deals with the isolation of Rhizobium bacteria from root nodules of Mimosa pudi... more The present study deals with the isolation of Rhizobium bacteria from root nodules of Mimosa pudica, and to check its efficiency as bio-fertilizer in improving plant growth. Rhizobium sps SN01 was isolated from root nodules of Mimosa pudica and confirmed to be Rhizobium bacteria through following biochemical tests of indole, methyl red, Voges-Proskauer and citrate utilization (IMViC), urease, hydrogen sulphide, carbohydrate fermentation tests. Rhizobium sps SN01 was analysed for antibiotic sensitivity test towards three different antibiotics such as Azithromycin, Amoxicillin and Cephalosporin. To check the efficiency, seeds of legume crops like cow peas, peas, and fenugreek were treated with Rhizobium sps, SN01, sowed in soil and coco-peat to study its germination rate and effectiveness in plant growth resulting in root length, shoot length, protein content and chlorophyll content. Impact of Rhizobium sps SN01 bacteria in soil and coco-peat quality and improvement was also focused by analyzing micro and macro nutrients responsible for its quality to improve plant growth.
Advances in Pharmacology and Pharmacy, 2021
Traditional Indian medicinal plant Simarouba glauca is a highly sacred plant known as "Paradise T... more Traditional Indian medicinal plant Simarouba glauca is a highly sacred plant known as "Paradise Tree or Lakshmi Taru" which is recognized for its pharmacological and pharmaceutical properties. The present study was envisaged to know the in vitro phytochemical, antioxidant and free radical scavenging activity of S. glauca seeds. Petroleum ether was used as a solvent to extract plant material using hot extraction method. In our investigation, phytochemical analysis was carried out qualitatively and quantitatively and for the evaluation of antioxidant profile carried out in vitro studies on free radical scavenging potential by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, decolourization potential of 2,2'-azino-bis[3-ethyl benzthiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant potential (FRAP) assay were determined in in vitro studies. The qualitative phytochemical analysis of this plant was positive for flavonoids, fatty acids, proteins, steroids and terpenoids and in quantitative analysis total flavonoid content was 17.32±0.12 mg/μg quercetin equivalent, total proanthocyanidin content exhibited 62.91±0.61 µg catechin equivalent, total phenol content exhibited 17.75±5.82 μg gallic acid equivalent and the total flavonol content was 1.54±0.01 µg quercetin equivalent of the extract. In the antioxidant profile, the maximum DPPH scavenging activity exhibited was 74% with an IC 50 value 137.89 µg/mL, the maximum ABTS scavenging activity exhibited was 35% with an IC 50 value 337.29 µg/mL and FRAP scavenging activity exhibited 62.5 µg/mL as ascorbic acid equivalents (AAE/ml) concentration. These findings evidenced that, the petroleum ether extract of S. glauca seeds has potential source of natural antioxidants and can be used it as therapeutic medicine.
International Journal of Current Pharmaceutical Research, 2016
Objective: Karnataka (India) is the darling child of nature where about 722 medicinal plants are ... more Objective: Karnataka (India) is the darling child of nature where about 722 medicinal plants are available. Ions of different metal elements have an important role in both the reactions. Few elements are essential to the body as nutrients called minerals. Present study deals with trace element analysis was carried out in Ballari district, Karnataka, India.Methods: 0.25g each of the powdered medicinal plant spice seeds samples digested in 6.5 ml of acid solution to study the trace element like Mn, Fe, Cu, Zn, Cd, and Cr in Coriander seeds, Ajwain seeds, Pepper seeds, Cumin seeds and Sweet cumin seeds at ppm levels were figured out by using AAS analysis.Results: Analysed trace mineral contents of these spice plants resulted highest level of Manganese is 1.92 ppm, Ferrous is 3.69 ppm and in Chromium is 0.0980 ppm in Ajwain seeds, Copper is 0.42 ppm in Coriander seeds, Zinc is 3.02 ppm and Cadmium is 0.0070 ppm in Sweet cumin seeds and lowest were found Mn is 0.65 ppm, Fe is 2.43 ppm in...
Springer International Publishing eBooks, 2022
Contraception: An Herbal Medicine Approach of Citrus medica Seeds for Fertility Regulation, this... more Contraception: An Herbal Medicine Approach of Citrus medica Seeds for Fertility Regulation, this research book focused on the evaluation of contraceptive activities of C. medica seeds on albino rats. Out of the tested three crude extracts, petroleum ether extract found more potential on fertility regulation, judged by phytochemical analysis, acute and chronic toxicity studies, antiimplantation, abortifacient, antiovulatory, estrogenic, progestogenic activities. Results were analysed by gravimetric, histological, histometrical, biochemical, antioxidant assays, hormonal profile by RIA and withdrawal studies with applying statistical analysis Student’s ‘t’ Test and also its TLC fractions were tested and found potential at the dose level of 50 and 100mg/kg/b.w. of fraction II in significantly. Spectroscopic analysis like UV, GCMS, IR and H1 NMR were resulted isolated compound is fatty acid mixture of fraction II, structure not elucidated and characterized due to the nature of fatty acid moiety. Hence, the fraction II is rich in fatty acid and shown contraceptive effects without obtrusive side effects in rats. Therefore, this fraction-II can be subjected for further study to harvest it, as a commercial contraceptive agent in near future.
Citrus medica Seeds for Fertility Regulation
Contraception: Citrus medica Seeds
International Journal of Pharmacy and Biological Sciences, 2019
Nanotechnology is a rapidly developing field, the purpose of which includes manufacturing new mat... more Nanotechnology is a rapidly developing field, the purpose of which includes manufacturing new materials at nano scale. The current technique of green synthesis of silver nanoparticles (AgNPs) is a progressive method used in medical science. An emerging problem for medical sector is multi drug resistance to antibiotics which is shown by wide range of bacteria pathogens. This study investigates an efficient and sustainable route of AgNPs preparation from 1mM aqueous AgNO3 using leaf extracts of seven plants Trachyspermum ammi (ajwain), Moringa oleifera (drumstick), Phyllanthus niruri (nela usiri), Tagetes erecta (marigold), Pimenta dioica (jamaica pepper), Hemigraphis alternata (red ivy) and Plectranthus purpuratus (vicks) well adorned for their wide availability and medicinal property. AgNPs were synthesized by the reaction of 1mM AgNO3 and 10% leaf extract of type of leaf separately. The solutions of AgNPs produced from plant extract were tested for its antibacterial activity. The AgNPs were characterized by UV visible spectrophotometer, X-ray Diffraction (XRD) and transmission electron microscopy (TEM). Fourier transform infrared spectrometer (FTIR) analysis was carried out to determine the nature of capping agents in each of these leaf extracts. AgNPs obtained showed significantly higher antimicrobial activity against Staphylococcus aureus, Klebsiella pneumoniae, Enterobacteriaceae. AgNPs from leaf extract of Trachyspermum ammi showed highest antibacterial activity on the MDRs used.