Kuldeep K. Lal | National Bureau of Fish Genetic resources (original) (raw)

Papers by Kuldeep K. Lal

Indian Journal of Fisheries, 2020

The length-weight relationship of a newly described catfish Pangasius silasi Dwivedi et. al., 201... more The length-weight relationship of a newly described catfish Pangasius silasi Dwivedi et. al., 2017 was studied from 88 specimens collected from the native distribution range of the species i.e. Nagarjuna Sagar Dam, Telengana, India. The total length and weight of the sampled fishes ranged from 290 to -590 mm and 240 to 2300 g respectively. Results of regression analysis showed that the regression coefficient (r²) values varied from 0.985 to 0.989. The average condition factor observed was 1.04 and 0.98 for males and females respectively. Positive allometric growth and condition factor of the species indicated the potential of the species for aquaculture. The information generated will also be useful in sustainable management and conservation of the species in natural waters.

PeerJ, 2020

Stem cell research has come into prominence because of its applications in assisted reproductive ... more Stem cell research has come into prominence because of its applications in assisted reproductive technology and the treatment of deadly diseases. In teleost fishes, spermatogonial stem cells have been effectively used to produce surrogate gametes and progeny through germ cell transplantation technique. The present study is the first report of an innovative application of stem cell therapy in fish species for revitalising the reproductive competence of senescent individuals. Senescent male goldfish, Carassius auratus, approximately 10 years of age were procured from a fish-breeding farm and were reared locally in the lab for an additional two years. The senescence of the individuals was then evaluated and confirmed using histological analysis, gonadal index assessment, and germ-cell specific vasa gene expression. Analyses revealed absence of spermatogonial cells and other germ cells in the testes of the senescent fish (n = 5). Spermatogonial cells from sexually immature C. auratus ma...

Mitochondrial DNA Part B, 2017

Systomus sarana sarana, commonly known as olive barb, is an important food and ornamental fish sp... more Systomus sarana sarana, commonly known as olive barb, is an important food and ornamental fish species with wide distribution in South Asia. Here, the complete mitogenome was sequenced on HiSeq 2500. With 16,590 nucleotides, the base composition was 32.9% (A), 26% (C), 15.4% (G) and 25.7% (T), comparable with other carps. The clustering pattern depicted the monophyly of S. sarana sarana with sister cyprinids.

Molecular Biology Reports, 2020

Genetic diversity and population structure in Indian featherback fish, Chitala chitala (Hamilton,... more Genetic diversity and population structure in Indian featherback fish, Chitala chitala (Hamilton, 1822) was investigated by combined analyses of two full mitochondrial genes, ATPase 6/8 and Cytochrome b. A total of 403 individuals, collected from 14 rivers yielded 61 haplotypes. Hierarchical partitioning analysis identified 19.01% variance 'among' and 80.99% variance 'within groups and populations'. The mean coefficient of genetic differentiation (F ST) was observed to be significant 0.26 (p < 0.05). Mantel tests rejected the hypothesis that genetic and geographic distances were correlated. The patterns of genetic differentiation, AMOVA and principal coordinate analyses indicated that natural populations were sub-structured and comprised of four genetic stocks of C. chitala in Indian rivers. The results also supported the higher resolution potential of concatenated gene sequences. The knowledge of genetic variation and divergence, from this study, can be utilized for its scientific conservation and management in the wild.

Aquaculture International, Apr 25, 2023

Journal of Applied Ichthyology, 2004

Forty-four primer sequences available for four cyprinid fishes were tested to amplify microsatell... more Forty-four primer sequences available for four cyprinid fishes were tested to amplify microsatellite loci in Indian major carp, Cirrhinus mrigala. A total of 12 loci were successfully amplified with clear scorable patterns and five thereof were polymorphic. Suitability of the identified ...

Thalassas: An International Journal of Marine Sciences

Bathymyrus simus, B. smithi and B. echinorhynchus are the three species recognised worldwide unde... more Bathymyrus simus, B. smithi and B. echinorhynchus are the three species recognised worldwide under the genus Bathymyrus. Of these, B. smithi and B. echinorhynchus have been reported in the Indian Ocean. Two specimens of Bathymyrus simus were collected from the Bay of Bengal, one from the by-catch of trawler at Nagapattinam fishing harbour, Tamil Nadu and another from the by-catch of trawler at Digha, West Bengal. The morphometric and meristic characters of the specimens were compared with the previous records of the Bathymyrus genus. It was reported that, the presence of dental rostral expansion has been attributed as a sexual dimorphic character in males; however the present female specimens with dental rostral expansion clearly indicates that it is not a sexual dimorphic character, but a species specific character. We report the presence of B. simus in the Indian Ocean, extending its known geographic range from the Western Pacific to the Indian Ocean.

Molecular biology reports, Jan 9, 2016

The stock characterization of wild populations of Silonia silondia is important for its scientifi... more The stock characterization of wild populations of Silonia silondia is important for its scientific management. At present, the information on genetic parameters of S. silondia is very limited. The species-specific microsatellite markers were developed in current study. The validated markers were used to genotype individuals from four distant rivers. To develop de novo microsatellite loci, an enriched genomic library was constructed for S. silondia using affinity-capture approach. The markers were validated for utility in population genetics. A total number of 76 individuals from four natural riverine populations were used to generate data for population analysis. The screening of isolated repeat sequences yielded eleven novel polymorphic microsatellite loci. The microsatellite loci exhibited high level of polymorphism, with 6-24 alleles per locus and the PIC value ranged from 0.604 to 0.927. The observed (Ho) and expected (He) heterozygosities ranged from 0.081 to 0.84 and 0.66 to 0...

Molecular biology reports, Jan 9, 2016

The stock characterization of wild populations of Silonia silondia is important for its scientifi... more The stock characterization of wild populations of Silonia silondia is important for its scientific management. At present, the information on genetic parameters of S. silondia is very limited. The species-specific microsatellite markers were developed in current study. The validated markers were used to genotype individuals from four distant rivers. To develop de novo microsatellite loci, an enriched genomic library was constructed for S. silondia using affinity-capture approach. The markers were validated for utility in population genetics. A total number of 76 individuals from four natural riverine populations were used to generate data for population analysis. The screening of isolated repeat sequences yielded eleven novel polymorphic microsatellite loci. The microsatellite loci exhibited high level of polymorphism, with 6-24 alleles per locus and the PIC value ranged from 0.604 to 0.927. The observed (Ho) and expected (He) heterozygosities ranged from 0.081 to 0.84 and 0.66 to 0...

Molecular Ecology Resources, Dec 10, 2012

27 polymorphic EST-SSRs were used to analyze two natural populations of Clarias batrachus for pop... more 27 polymorphic EST-SSRs were used to analyze two natural populations of Clarias batrachus for population variability parameters. Allele numbers ranged from 2 to 12; and observed and expected heterozygosity values from 0.3658 and 0.6850; and 0.5668 and 0.5828, respectively. Fifteen loci showed no significant departure from HWE (p<0.05). Linkage disequilibrium test was significant for three pairs of loci. Two populations studied were found to be significantly divergent. Cross-species amplification for 7 loci was successful in two related species. These SSRs markers may be useful for the assessment of genetic variations and population differentiation studies in C. batrachus.

Abstract Research on fish reproduction has progressed considerably during the last few years owin... more Abstract Research on fish reproduction has progressed considerably during the last few years owing to the development of new and varied techniques. However, its application to acquaculture has largely been confined to a few selected species for quite a long time. ...

Genetic structure of the anadromous clupeid hilsa shad (Tenualosa ilisha) in Ganges was investiga... more Genetic structure of the anadromous clupeid hilsa shad (Tenualosa ilisha) in Ganges was investigated using allozyme markers. A total of 26 loci are genotyped for samples collected during spawning period from five riverine locations viz. Brahmaputra, Padma, Ganga, Hoogly and Feeder canal. Despite the high level of polymorphism (31-50%, most common allele < 0.99), the samples from different locations do not exhibit significant genetic heterogeneity. The present results do not support earlier reports of the possibility of different races of hilsa in Ganges, but provide evidence for the existence of a single panmictic population. The observed homogeneity of allele frequencies is most likely to be explained by gene flow between the hilsa populations ascending different channels of Ganges.

Indian Journal of Fisheries

Spermatozoa of rohu (Laheo rohita) were diluted in two cryodiluents and fine structural changes w... more Spermatozoa of rohu (Laheo rohita) were diluted in two cryodiluents and fine structural changes were investigated. Damages were observed in head, mid-piece and tail of about 51% spermatozoa immediately after dilution in one of the cryodiluents. After 10 minutes of equilibration period, the frequency of damaged sperms increased to 85%. The other diluent did not exhibit significant structural changes unlike the former one and nearly 60% sperms remained visibly intact even after 10 minutes of equilibration time. The present investigation points out (1) diluents inhibiting sperm motility alone need not always be ideal for a species and (2) usefulness of ultrastructural images offish sperms in determining the fertilizing ability of milt and in preliminary screening and selection of cryodiluents for deepfreezing programmes.

Fourteen polymorphic allozyme loci were identified in yellow catfish, Horabagrus brachysoma. The ... more Fourteen polymorphic allozyme loci were identified in yellow catfish, Horabagrus brachysoma. The genetic variation detected at each allozyme locus was assessed for samples collected from three rivers. The observed heterozygosities per locus ranged from 0.0286 to 0.4000. Significant genotype heterogeneity indicated that the samples are not drawn from same gene pool. The results suggest the potential of the identified loci to analyze stock structure of natural populations of H. brachysoma.

In India and other developing countries, the role of institutional support and social connectedne... more In India and other developing countries, the role of institutional support and social connectedness among fishing communities is played by and large by conventional fishermen groups, fishermen cooperative societies, federations, etc. These organizations by their very nature and reason for existence are aimed at increasing the maximum output from the fishery resources and thereby, increasing the livelihood, income and wellbeing of their members. In India, fishing cooperative societies have been studied by very few researchers and most of these studies have focused on performance of fishing cooperatives on production, marketing and financial aspects. Fishing cooperative societies as a social organization have not adequately been studied in India. This paper reports findings from a study undertaken to assess the internal functioning of the fishing cooperative societies established and promoted under different scenarios. The study covered 58 fishing cooperative societies in three states viz. Himachal Pradesh, Madhya Pradesh and Uttar Pradesh.

Indian Journal of Fisheries, 2020

The length-weight relationship of a newly described catfish Pangasius silasi Dwivedi et. al., 201... more The length-weight relationship of a newly described catfish Pangasius silasi Dwivedi et. al., 2017 was studied from 88 specimens collected from the native distribution range of the species i.e. Nagarjuna Sagar Dam, Telengana, India. The total length and weight of the sampled fishes ranged from 290 to -590 mm and 240 to 2300 g respectively. Results of regression analysis showed that the regression coefficient (r²) values varied from 0.985 to 0.989. The average condition factor observed was 1.04 and 0.98 for males and females respectively. Positive allometric growth and condition factor of the species indicated the potential of the species for aquaculture. The information generated will also be useful in sustainable management and conservation of the species in natural waters.

PeerJ, 2020

Stem cell research has come into prominence because of its applications in assisted reproductive ... more Stem cell research has come into prominence because of its applications in assisted reproductive technology and the treatment of deadly diseases. In teleost fishes, spermatogonial stem cells have been effectively used to produce surrogate gametes and progeny through germ cell transplantation technique. The present study is the first report of an innovative application of stem cell therapy in fish species for revitalising the reproductive competence of senescent individuals. Senescent male goldfish, Carassius auratus, approximately 10 years of age were procured from a fish-breeding farm and were reared locally in the lab for an additional two years. The senescence of the individuals was then evaluated and confirmed using histological analysis, gonadal index assessment, and germ-cell specific vasa gene expression. Analyses revealed absence of spermatogonial cells and other germ cells in the testes of the senescent fish (n = 5). Spermatogonial cells from sexually immature C. auratus ma...

Mitochondrial DNA Part B, 2017

Systomus sarana sarana, commonly known as olive barb, is an important food and ornamental fish sp... more Systomus sarana sarana, commonly known as olive barb, is an important food and ornamental fish species with wide distribution in South Asia. Here, the complete mitogenome was sequenced on HiSeq 2500. With 16,590 nucleotides, the base composition was 32.9% (A), 26% (C), 15.4% (G) and 25.7% (T), comparable with other carps. The clustering pattern depicted the monophyly of S. sarana sarana with sister cyprinids.

Molecular Biology Reports, 2020

Genetic diversity and population structure in Indian featherback fish, Chitala chitala (Hamilton,... more Genetic diversity and population structure in Indian featherback fish, Chitala chitala (Hamilton, 1822) was investigated by combined analyses of two full mitochondrial genes, ATPase 6/8 and Cytochrome b. A total of 403 individuals, collected from 14 rivers yielded 61 haplotypes. Hierarchical partitioning analysis identified 19.01% variance 'among' and 80.99% variance 'within groups and populations'. The mean coefficient of genetic differentiation (F ST) was observed to be significant 0.26 (p < 0.05). Mantel tests rejected the hypothesis that genetic and geographic distances were correlated. The patterns of genetic differentiation, AMOVA and principal coordinate analyses indicated that natural populations were sub-structured and comprised of four genetic stocks of C. chitala in Indian rivers. The results also supported the higher resolution potential of concatenated gene sequences. The knowledge of genetic variation and divergence, from this study, can be utilized for its scientific conservation and management in the wild.

Aquaculture International, Apr 25, 2023

Journal of Applied Ichthyology, 2004

Forty-four primer sequences available for four cyprinid fishes were tested to amplify microsatell... more Forty-four primer sequences available for four cyprinid fishes were tested to amplify microsatellite loci in Indian major carp, Cirrhinus mrigala. A total of 12 loci were successfully amplified with clear scorable patterns and five thereof were polymorphic. Suitability of the identified ...

Thalassas: An International Journal of Marine Sciences

Bathymyrus simus, B. smithi and B. echinorhynchus are the three species recognised worldwide unde... more Bathymyrus simus, B. smithi and B. echinorhynchus are the three species recognised worldwide under the genus Bathymyrus. Of these, B. smithi and B. echinorhynchus have been reported in the Indian Ocean. Two specimens of Bathymyrus simus were collected from the Bay of Bengal, one from the by-catch of trawler at Nagapattinam fishing harbour, Tamil Nadu and another from the by-catch of trawler at Digha, West Bengal. The morphometric and meristic characters of the specimens were compared with the previous records of the Bathymyrus genus. It was reported that, the presence of dental rostral expansion has been attributed as a sexual dimorphic character in males; however the present female specimens with dental rostral expansion clearly indicates that it is not a sexual dimorphic character, but a species specific character. We report the presence of B. simus in the Indian Ocean, extending its known geographic range from the Western Pacific to the Indian Ocean.

Molecular biology reports, Jan 9, 2016

The stock characterization of wild populations of Silonia silondia is important for its scientifi... more The stock characterization of wild populations of Silonia silondia is important for its scientific management. At present, the information on genetic parameters of S. silondia is very limited. The species-specific microsatellite markers were developed in current study. The validated markers were used to genotype individuals from four distant rivers. To develop de novo microsatellite loci, an enriched genomic library was constructed for S. silondia using affinity-capture approach. The markers were validated for utility in population genetics. A total number of 76 individuals from four natural riverine populations were used to generate data for population analysis. The screening of isolated repeat sequences yielded eleven novel polymorphic microsatellite loci. The microsatellite loci exhibited high level of polymorphism, with 6-24 alleles per locus and the PIC value ranged from 0.604 to 0.927. The observed (Ho) and expected (He) heterozygosities ranged from 0.081 to 0.84 and 0.66 to 0...

Molecular biology reports, Jan 9, 2016

The stock characterization of wild populations of Silonia silondia is important for its scientifi... more The stock characterization of wild populations of Silonia silondia is important for its scientific management. At present, the information on genetic parameters of S. silondia is very limited. The species-specific microsatellite markers were developed in current study. The validated markers were used to genotype individuals from four distant rivers. To develop de novo microsatellite loci, an enriched genomic library was constructed for S. silondia using affinity-capture approach. The markers were validated for utility in population genetics. A total number of 76 individuals from four natural riverine populations were used to generate data for population analysis. The screening of isolated repeat sequences yielded eleven novel polymorphic microsatellite loci. The microsatellite loci exhibited high level of polymorphism, with 6-24 alleles per locus and the PIC value ranged from 0.604 to 0.927. The observed (Ho) and expected (He) heterozygosities ranged from 0.081 to 0.84 and 0.66 to 0...

Molecular Ecology Resources, Dec 10, 2012

27 polymorphic EST-SSRs were used to analyze two natural populations of Clarias batrachus for pop... more 27 polymorphic EST-SSRs were used to analyze two natural populations of Clarias batrachus for population variability parameters. Allele numbers ranged from 2 to 12; and observed and expected heterozygosity values from 0.3658 and 0.6850; and 0.5668 and 0.5828, respectively. Fifteen loci showed no significant departure from HWE (p<0.05). Linkage disequilibrium test was significant for three pairs of loci. Two populations studied were found to be significantly divergent. Cross-species amplification for 7 loci was successful in two related species. These SSRs markers may be useful for the assessment of genetic variations and population differentiation studies in C. batrachus.

Abstract Research on fish reproduction has progressed considerably during the last few years owin... more Abstract Research on fish reproduction has progressed considerably during the last few years owing to the development of new and varied techniques. However, its application to acquaculture has largely been confined to a few selected species for quite a long time. ...

Genetic structure of the anadromous clupeid hilsa shad (Tenualosa ilisha) in Ganges was investiga... more Genetic structure of the anadromous clupeid hilsa shad (Tenualosa ilisha) in Ganges was investigated using allozyme markers. A total of 26 loci are genotyped for samples collected during spawning period from five riverine locations viz. Brahmaputra, Padma, Ganga, Hoogly and Feeder canal. Despite the high level of polymorphism (31-50%, most common allele < 0.99), the samples from different locations do not exhibit significant genetic heterogeneity. The present results do not support earlier reports of the possibility of different races of hilsa in Ganges, but provide evidence for the existence of a single panmictic population. The observed homogeneity of allele frequencies is most likely to be explained by gene flow between the hilsa populations ascending different channels of Ganges.

Indian Journal of Fisheries

Spermatozoa of rohu (Laheo rohita) were diluted in two cryodiluents and fine structural changes w... more Spermatozoa of rohu (Laheo rohita) were diluted in two cryodiluents and fine structural changes were investigated. Damages were observed in head, mid-piece and tail of about 51% spermatozoa immediately after dilution in one of the cryodiluents. After 10 minutes of equilibration period, the frequency of damaged sperms increased to 85%. The other diluent did not exhibit significant structural changes unlike the former one and nearly 60% sperms remained visibly intact even after 10 minutes of equilibration time. The present investigation points out (1) diluents inhibiting sperm motility alone need not always be ideal for a species and (2) usefulness of ultrastructural images offish sperms in determining the fertilizing ability of milt and in preliminary screening and selection of cryodiluents for deepfreezing programmes.

Fourteen polymorphic allozyme loci were identified in yellow catfish, Horabagrus brachysoma. The ... more Fourteen polymorphic allozyme loci were identified in yellow catfish, Horabagrus brachysoma. The genetic variation detected at each allozyme locus was assessed for samples collected from three rivers. The observed heterozygosities per locus ranged from 0.0286 to 0.4000. Significant genotype heterogeneity indicated that the samples are not drawn from same gene pool. The results suggest the potential of the identified loci to analyze stock structure of natural populations of H. brachysoma.

In India and other developing countries, the role of institutional support and social connectedne... more In India and other developing countries, the role of institutional support and social connectedness among fishing communities is played by and large by conventional fishermen groups, fishermen cooperative societies, federations, etc. These organizations by their very nature and reason for existence are aimed at increasing the maximum output from the fishery resources and thereby, increasing the livelihood, income and wellbeing of their members. In India, fishing cooperative societies have been studied by very few researchers and most of these studies have focused on performance of fishing cooperatives on production, marketing and financial aspects. Fishing cooperative societies as a social organization have not adequately been studied in India. This paper reports findings from a study undertaken to assess the internal functioning of the fishing cooperative societies established and promoted under different scenarios. The study covered 58 fishing cooperative societies in three states viz. Himachal Pradesh, Madhya Pradesh and Uttar Pradesh.