rajiv sah | Northeast Normal University (original) (raw)

Papers by rajiv sah

International Immunopharmacology

Electronic Journal of Biotechnology, 2021

Background Many human genetic diseases arise from point mutations. These genetic diseases can the... more Background Many human genetic diseases arise from point mutations. These genetic diseases can theoretically be corrected through gene therapy. However, gene therapy in clinical application is still far from mature. Nearly half of the pathogenic single-nucleotide polymorphisms (SNPs) are caused by G:C>A:T or T:A>C:G base changes and the ideal approaches to correct these mutations are base editing. These CRISPR-Cas9-mediated base editing does not leave any footprint in genome and does not require donor DNA sequences for homologous recombination. These base editing methods have been successfully applied to cultured mammalian cells with high precision and efficiency, but BE4 has not been confirmed in mice. Animal models are important for dissecting pathogenic mechanism of human genetic diseases and testing of base correction efficacy in vivo. Cytidine base editor BE4 is a newly developed version of cytidine base editing system that converts cytidine (C) to uridine (U). Results In ...

Transgenic Research, 2021

Vascular endothelial growth factor (VEGF) is important for lung development and function but idea... more Vascular endothelial growth factor (VEGF) is important for lung development and function but ideal mouse models are limited to decipher the quantitative relationship between VEGF expression levels and its proper development and pathogenesis. Human SPC promoter has been used to faithfully express genes or cDNAs in the pulmonary epithelium in many transgenic mouse models. In the study, a mouse model of lung-specific and reversible VEGF repression ( hspc - rtTR tg/ + /Vegf tetO/tetO ) was generated. Human SPC promoter was used to drive lung-specific rtTR expression, a cDNA coding for doxycycline-regulated transcription repression protein. By crossing with Vegf tetO/tetO mice, that has tetracycline operator sequences insertion in 5′-UTR region, it allows us to reversibly inhibit lung VEGF transcription from its endogenous level through doxycycline food, water or injection. The tissue-specific inhibition of VEGF is used to mimic abnormal expression levels of VEGF in lung. Reduced VEGF ex...

Electricity can be generated from naturally occurring electro potential differences through the b... more Electricity can be generated from naturally occurring electro potential differences through the burial of an anode in sediment and the immersion of a cathode in the overlying body of water. Such a set-up is called a Sediment microbial fuel cell (SMFC) or benthic MFC. Mangrove forest sediment of ―Sundarbans‖ was used to construct SMFC and an open circuit voltage was measured over 40 days. Voltage was found to increase over 14 days after which the voltage remained steady for about 7 days and then declined. The highest voltage of 610 mV was obtained from a single SMFC on the 24th day. Comparative analysis using earthen pot as a proton exchange membrane showed the voltage to increase gradually over an initial period of time, with a highest increase in 80 mV over SMFC without earthen pot. An analysis of different cathode material showed that the highest voltage was obtained from carbon rod over carbon brush and stainless steel as cathode material. Several SMFC were added to make a se...

Current issues in molecular biology, 2021

Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily ... more Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily and plays an essential role in axonal outgrowth during embryogenesis. In adults, Dip2B is highly expressed in different brain regions, as shown by in situ analysis, and may have a role in axon guidance. However, the expression and biological role of Dip2B in other somatic tissues remain unknown. To better visualize Dip2B expression and to provide insight into the roles of Dip2B during postnatal development, we used a Dip2btm1a(wtsi)komp knock-in mouse model, in which a LacZ-Neo fusion protein is expressed under Dip2b promoter and allowed Dip2B expression to be analyzed by X-gal staining. qPCR analyses showed that Dip2b mRNA was expressed in a variety of somatic tissues, including lung and kidney, in addition to brain. LacZ staining indicated that Dip2B is broadly expressed in neuronal, reproductive, and vascular tissues as well as in the kidneys, heart, liver, and lungs. Moreover, neuron...

Computational and Structural Biotechnology Journal

International Journal of Molecular Sciences

Molecular and anatomical functions of mammalian Dip2 family members (Dip2A, Dip2B and Dip2C) duri... more Molecular and anatomical functions of mammalian Dip2 family members (Dip2A, Dip2B and Dip2C) during organogenesis are largely unknown. Here, we explored the indispensable role of Dip2B in mouse lung development. Using a LacZ reporter, we explored Dip2B expression during embryogenesis. This study shows that Dip2B expression is widely distributed in various neuronal, myocardial, endothelial, and epithelial cell types during embryogenesis. Target disruption of Dip2b leads to intrauterine growth restriction, defective lung formation and perinatal mortality. Dip2B is crucial for late lung maturation rather than early-branching morphogenesis. The morphological analysis shows that Dip2b loss leads to disrupted air sac formation, interstitium septation and increased cellularity. In BrdU incorporation assay, it is shown that Dip2b loss results in increased cell proliferation at the saccular stage of lung development. RNA-seq analysis reveals that 1431 genes are affected in Dip2b deficient lu...

Disconnected (disco)-interacting protein 2 homolog A is a member of the DIP2 protein family encod... more Disconnected (disco)-interacting protein 2 homolog A is a member of the DIP2 protein family
encoded by Dip2a gene. Dip2a expression pattern has never been systematically studied.
Functions of Dip2a in embryonic development and adult are not known. To investigate
Dip2a gene expression and function in embryo and adult, a Dip2a-LacZ mouse model was
generated by insertion of β-Gal cDNA after Dip2a promoter using CRISPR/Cas9 technology.
Dip2a-LacZ mouse was designed to be a lacZ reporter mouse as well as a Dip2a
knockout mouse. Heterozygous mice were used to study endogenous Dip2a expression
and homozygotes to study DIP2A-associated structure and function. LacZ staining indicated
that Dip2a is broadly expressed in neuronal, reproductive and vascular tissues, as
well as in heart, kidney, liver and lung. Results demonstrate that Dip2a is expressed in ectoderm-
derived tissues in developing embryos. Adult tissues showed rich staining in neurons,
mesenchymal, endothelial, smooth muscle cells and cardiomyocytes by cell types. The
expression pattern highly overlaps with FSTL1 and supports previous report that DIP2A to
be potential receptor of FSTL1 and its protective roles of cardiomyocytes. Broad and
intense embryonic and adult expression of Dip2a has implied their multiple structural and
physiological roles.

Electricity can be generated from naturally occurring electro potential differences through the b... more Electricity can be generated from naturally occurring electro potential differences through the burial of an
anode in sediment and the immersion of a cathode in the overlying body of water. Such a set-up is called a Sediment
microbial fuel cell (SMFC) or benthic MFC. Mangrove forest sediment of ―Sundarbans‖ was used to construct SMFC
and an open circuit voltage was measured over 40 days. Voltage was found to increase over 14 days after which the
voltage remained steady for about 7 days and then declined. The highest voltage of 610 mV was obtained from a single
SMFC on the 24th day. Comparative analysis using earthen pot as a proton exchange membrane showed the voltage to
increase gradually over an initial period of time, with a highest increase in 80 mV over SMFC without earthen pot. An
analysis of different cathode material showed that the highest voltage was obtained from carbon rod over carbon brush
and stainless steel as cathode material. Several SMFC were added to make a series connection, which gave enough
voltage (3.8 volts) to turn-on a LED bulb and a scientific calculator. The study suggests that Sundarban soil sediment
can be a potential source for further study in the development of SMFC.

International Immunopharmacology

Electronic Journal of Biotechnology, 2021

Background Many human genetic diseases arise from point mutations. These genetic diseases can the... more Background Many human genetic diseases arise from point mutations. These genetic diseases can theoretically be corrected through gene therapy. However, gene therapy in clinical application is still far from mature. Nearly half of the pathogenic single-nucleotide polymorphisms (SNPs) are caused by G:C>A:T or T:A>C:G base changes and the ideal approaches to correct these mutations are base editing. These CRISPR-Cas9-mediated base editing does not leave any footprint in genome and does not require donor DNA sequences for homologous recombination. These base editing methods have been successfully applied to cultured mammalian cells with high precision and efficiency, but BE4 has not been confirmed in mice. Animal models are important for dissecting pathogenic mechanism of human genetic diseases and testing of base correction efficacy in vivo. Cytidine base editor BE4 is a newly developed version of cytidine base editing system that converts cytidine (C) to uridine (U). Results In ...

Transgenic Research, 2021

Vascular endothelial growth factor (VEGF) is important for lung development and function but idea... more Vascular endothelial growth factor (VEGF) is important for lung development and function but ideal mouse models are limited to decipher the quantitative relationship between VEGF expression levels and its proper development and pathogenesis. Human SPC promoter has been used to faithfully express genes or cDNAs in the pulmonary epithelium in many transgenic mouse models. In the study, a mouse model of lung-specific and reversible VEGF repression ( hspc - rtTR tg/ + /Vegf tetO/tetO ) was generated. Human SPC promoter was used to drive lung-specific rtTR expression, a cDNA coding for doxycycline-regulated transcription repression protein. By crossing with Vegf tetO/tetO mice, that has tetracycline operator sequences insertion in 5′-UTR region, it allows us to reversibly inhibit lung VEGF transcription from its endogenous level through doxycycline food, water or injection. The tissue-specific inhibition of VEGF is used to mimic abnormal expression levels of VEGF in lung. Reduced VEGF ex...

Electricity can be generated from naturally occurring electro potential differences through the b... more Electricity can be generated from naturally occurring electro potential differences through the burial of an anode in sediment and the immersion of a cathode in the overlying body of water. Such a set-up is called a Sediment microbial fuel cell (SMFC) or benthic MFC. Mangrove forest sediment of ―Sundarbans‖ was used to construct SMFC and an open circuit voltage was measured over 40 days. Voltage was found to increase over 14 days after which the voltage remained steady for about 7 days and then declined. The highest voltage of 610 mV was obtained from a single SMFC on the 24th day. Comparative analysis using earthen pot as a proton exchange membrane showed the voltage to increase gradually over an initial period of time, with a highest increase in 80 mV over SMFC without earthen pot. An analysis of different cathode material showed that the highest voltage was obtained from carbon rod over carbon brush and stainless steel as cathode material. Several SMFC were added to make a se...

Current issues in molecular biology, 2021

Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily ... more Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily and plays an essential role in axonal outgrowth during embryogenesis. In adults, Dip2B is highly expressed in different brain regions, as shown by in situ analysis, and may have a role in axon guidance. However, the expression and biological role of Dip2B in other somatic tissues remain unknown. To better visualize Dip2B expression and to provide insight into the roles of Dip2B during postnatal development, we used a Dip2btm1a(wtsi)komp knock-in mouse model, in which a LacZ-Neo fusion protein is expressed under Dip2b promoter and allowed Dip2B expression to be analyzed by X-gal staining. qPCR analyses showed that Dip2b mRNA was expressed in a variety of somatic tissues, including lung and kidney, in addition to brain. LacZ staining indicated that Dip2B is broadly expressed in neuronal, reproductive, and vascular tissues as well as in the kidneys, heart, liver, and lungs. Moreover, neuron...

Computational and Structural Biotechnology Journal

International Journal of Molecular Sciences

Molecular and anatomical functions of mammalian Dip2 family members (Dip2A, Dip2B and Dip2C) duri... more Molecular and anatomical functions of mammalian Dip2 family members (Dip2A, Dip2B and Dip2C) during organogenesis are largely unknown. Here, we explored the indispensable role of Dip2B in mouse lung development. Using a LacZ reporter, we explored Dip2B expression during embryogenesis. This study shows that Dip2B expression is widely distributed in various neuronal, myocardial, endothelial, and epithelial cell types during embryogenesis. Target disruption of Dip2b leads to intrauterine growth restriction, defective lung formation and perinatal mortality. Dip2B is crucial for late lung maturation rather than early-branching morphogenesis. The morphological analysis shows that Dip2b loss leads to disrupted air sac formation, interstitium septation and increased cellularity. In BrdU incorporation assay, it is shown that Dip2b loss results in increased cell proliferation at the saccular stage of lung development. RNA-seq analysis reveals that 1431 genes are affected in Dip2b deficient lu...

Disconnected (disco)-interacting protein 2 homolog A is a member of the DIP2 protein family encod... more Disconnected (disco)-interacting protein 2 homolog A is a member of the DIP2 protein family
encoded by Dip2a gene. Dip2a expression pattern has never been systematically studied.
Functions of Dip2a in embryonic development and adult are not known. To investigate
Dip2a gene expression and function in embryo and adult, a Dip2a-LacZ mouse model was
generated by insertion of β-Gal cDNA after Dip2a promoter using CRISPR/Cas9 technology.
Dip2a-LacZ mouse was designed to be a lacZ reporter mouse as well as a Dip2a
knockout mouse. Heterozygous mice were used to study endogenous Dip2a expression
and homozygotes to study DIP2A-associated structure and function. LacZ staining indicated
that Dip2a is broadly expressed in neuronal, reproductive and vascular tissues, as
well as in heart, kidney, liver and lung. Results demonstrate that Dip2a is expressed in ectoderm-
derived tissues in developing embryos. Adult tissues showed rich staining in neurons,
mesenchymal, endothelial, smooth muscle cells and cardiomyocytes by cell types. The
expression pattern highly overlaps with FSTL1 and supports previous report that DIP2A to
be potential receptor of FSTL1 and its protective roles of cardiomyocytes. Broad and
intense embryonic and adult expression of Dip2a has implied their multiple structural and
physiological roles.

Electricity can be generated from naturally occurring electro potential differences through the b... more Electricity can be generated from naturally occurring electro potential differences through the burial of an
anode in sediment and the immersion of a cathode in the overlying body of water. Such a set-up is called a Sediment
microbial fuel cell (SMFC) or benthic MFC. Mangrove forest sediment of ―Sundarbans‖ was used to construct SMFC
and an open circuit voltage was measured over 40 days. Voltage was found to increase over 14 days after which the
voltage remained steady for about 7 days and then declined. The highest voltage of 610 mV was obtained from a single
SMFC on the 24th day. Comparative analysis using earthen pot as a proton exchange membrane showed the voltage to
increase gradually over an initial period of time, with a highest increase in 80 mV over SMFC without earthen pot. An
analysis of different cathode material showed that the highest voltage was obtained from carbon rod over carbon brush
and stainless steel as cathode material. Several SMFC were added to make a series connection, which gave enough
voltage (3.8 volts) to turn-on a LED bulb and a scientific calculator. The study suggests that Sundarban soil sediment
can be a potential source for further study in the development of SMFC.