Sanja Stojanovic | University of Niš (original) (raw)

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Papers by Sanja Stojanovic

Research paper thumbnail of The effect of hydrogel poly (ethylene glycol) dimethacrylates on the viability and proliferation

Research paper thumbnail of Application of MTT viability test for biocompatibility examination of N-CP/DLPLG nanoparticles on cell cultures

Research paper thumbnail of Propolis effect on morphology of human gingival fibroblasts in vitro

Research paper thumbnail of In vitro osteogenic differentiation and phenotypisiation of mouse adipose tissue derived mesenchymal cells

Research paper thumbnail of Uporedni Pregled Sredstava Za Retrakciju Gingive

Acta medica medianae, 2013

In order to obtain an adequate impression of demarcation line area of a prepared tooth located in... more In order to obtain an adequate impression of demarcation line area of a prepared tooth located in or below the gingival edge it is necessary to perform the dilatation and drying of the gingival sulcus using retraction cord impregnated with adequate retraction agents. The aim of the study was to carry out comparative analysis of advantages and disadvantages of commercially available gingival retraction agents. Commercial retraction agents include astringents (metal salts) and vasoconstrictors on the basis of epinephrine. Further research should be aimed at examining the possibility of using sympathomimetic vasoconstrictors (tetrahydrosolin and oxymetasolin) for gingival retraction. Acta Medica Medianae 2012; 51(1):81-84.

Research paper thumbnail of Comparative Review of Gingival Retraction Agents

Acta medica medianae, 2012

In order to obtain an adequate impression of demarcation line area of a prepared tooth located in... more In order to obtain an adequate impression of demarcation line area of a prepared tooth located in or below the gingival edge it is necessary to perform the dilatation and drying of the gingival sulcus using retraction cord impregnated with adequate retraction agents. The aim of the study was to carry out comparative analysis of advantages and disadvantages of commercially available gingival retraction agents. Commercial retraction agents include astringents (metal salts) and vasoconstrictors on the basis of epinephrine. Further research should be aimed at examining the possibility of using sympathomimetic vasoconstrictors (tetrahydrosolin and oxymetasolin) for gingival retraction. Acta Medica Medianae 2012; 51(1):81-84.

Research paper thumbnail of Addition of blood to a phycogenic bone substitute leads to increased in vivo vascularization

The present study aimed to analyze the effects of the addition of blood to the phycogenic bone su... more The present study aimed to analyze the effects of the addition of blood to the phycogenic bone substitute Algipore ® on the severity of in vivo tissue reaction. Initially, Fourier-transform infrared spectroscopy (FTIR) of the bone substitute was conducted to analyze its chemical composition. The subcutaneous implantation model in Balb/c mice was then applied for up to 30 d to analyze the tissue reactions on the basis of specialized histochemical, immunohistochemical, and histomorphometrical methods.

Research paper thumbnail of In vitro effects of Royal jelly on viability and proliferation of HeLa cells

Research paper thumbnail of Osteogenic potential of adipose-derived mesenchymal stem cells in the case of osteoporosis

Osteoporosis is a chronic progressive disease characterized by reduced bone mineral density and b... more Osteoporosis is a chronic progressive disease characterized by reduced bone mineral density and bone mass which leads to increased risk of developing bone fractures. Diseases that are commonly associated with osteoporosis, or can contribute to its development are type 2 diabetes mellitus and asthma. In this study we examined the potential of adipose-derived mesenchymal stem cells (ADSCs) to proliferate and to differentiate into osteoblasts in vitro from a female patient who is suffering from osteoporosis, type 2 diabetes mellitus and asthma, and who obtained bilateral subtrochanteric femur fractures. Subcutaneous adipose tissue close to the fracture was taken during the surgical procedure of osteosynthesis and mesenchymal stem cells were isolated by digestion of the tissue and subsequently cultivated in vitro. After the second passage proliferation assay was performed and cells were cultivated in an osteogenic differentiation medium for a period of 21 days. Using cytochemical and immunocytochemical methods we have shown that these cells have the potential to differentiate into osteoblasts. These results suggest that adipose-derived mesenchymal stem cells obtained from patients with aforementioned chronic diseases can be used in tissue engineering for reparation of bone defects.

Research paper thumbnail of The effect of macrophages on initiation of osteogenesis in subcutaneous implants

Research paper thumbnail of Spontaneous Chromosomal Instability Related to Aging and Gender

Research paper thumbnail of Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions

Research paper thumbnail of Osteogenic potential of freshly isolated cells of adipose-derived mesenchymal fraction applied with nanoparticles

The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tis... more The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.

Research paper thumbnail of 1-Poster PhysChem2014 FTIR SEM-EDX

Research paper thumbnail of Neovasculogenic and osteogenic potential of adipose-derived stem cells implanted in combination with bone mineral matrix and platelet-rich plasma at ectopic site

Research paper thumbnail of Osteo-regenerative potential of adipose tissue-derived mesenchymal stem cells

Research paper thumbnail of Adipose derived mesenchymal stem cells as a model for study osteoinductive activity of bone substituting biomaterials

Adipose derived mesenchymal stem cells (ADMSC) show remarkable plasticity because of their potent... more Adipose derived mesenchymal stem cells (ADMSC) show remarkable plasticity because of their potential for differentiating into various cell types, including cells which are characteristic for osteogenic process, such as osteoblasts and endothelial cells. Their cultivation on biomaterial scaffolds is a common approach in tissue engineering. In our previous in vivo studies we used ADMSC for implantation into the experimental animals onto the bone substituting biomaterials as carriers. Our in vitro studies showed that cultivation of human and murine ADMSC in normal and osteoinductive medium with or without biomaterials can be a good model for assessing their bone substituting potential.

Research paper thumbnail of Histochemical Analysis of In vivo Osteogenic Processes in Constructs Consisted of Adipose-Derived Stem Cells, Platelet-rich Plasma and Bone Mineral Matrix

Cell-based bone tissue engineering is considered as promising approach in bone defects healing. T... more Cell-based bone tissue engineering is considered as promising approach in bone defects healing. Three basic components in this approach are osteoprogenitor cells, sources of growth and proliferation factors and bone substitute material as carrier for previous two elements. Aim of this study was to evaluate in vivo osteogenic potential of Adipose-derived Stem Cells (ADSCs) delivered on bone mineral matrix granules with addition of platelet-rich plasma (PRP). ADSCs isolated from supra-epididymal fat pads of Balb/c mice were subjected to in vitro osteoinduction for fifteen days before constructs were prepared. Prepared constructs were implanted subcutaneously in Balb/c mice. Histochemistry analysis was performed on implants extracted two and eight weeks after implantation. According to obtained results, this approach could be of benefit for bone tissue engineering purposes. Keywords: bone mineral matrix, ADSC, bone tissue engineering, PRP, osteoinduction Acknowledgements: This work was...

Research paper thumbnail of Procena produkcije slobodnih radikala kiseonika u peritonealnim makrofagima miša semikvantitativnim mikroskopskim NBT testom

Research paper thumbnail of Examination of In vitro Neovasculogenic Potential of Mice Adipose-derived Stem Cells

Examination of In vitro Neovasculogenic Potential of Mice Adipose-derived Stem Cells Jelena Najda... more Examination of In vitro Neovasculogenic Potential of Mice Adipose-derived Stem Cells Jelena Najdanović1, Vladimir Cvetković2, Marija Vukelić-Nikolić1, Jelena Živković1, Sanja Stojanović1, Stevo Najman1 1Faculty of Medicine, University of Nis, Bul. dr Zorana Đinđića 81, Niš, Serbia 2Faculty of Sciences and Mathematics, University of Nis, Višegradska 33, Niš, Serbia Bone tissue regeneration depends on oxygen and essential nutrients supply which is mediated by blood vessels. Therefore, neovasculogenesis and angiogenesis are of great importance in bone tissue engineering. The aim of this study was to examine neovasculogenic potential of adipose-derived stem cells (ADSC) subjected to endothelogenesis in vitro. ADSCs were isolated from mice supraepididymal adipose tissue, expanded in proliferative media and subjected to endothelogenic differentiation for fifteen days. Relative expression levels of endothelial-related gene markers: VCAM, Flt-1 and Egr-1 were monitored applying quantitative...

Research paper thumbnail of The effect of hydrogel poly (ethylene glycol) dimethacrylates on the viability and proliferation

Research paper thumbnail of Application of MTT viability test for biocompatibility examination of N-CP/DLPLG nanoparticles on cell cultures

Research paper thumbnail of Propolis effect on morphology of human gingival fibroblasts in vitro

Research paper thumbnail of In vitro osteogenic differentiation and phenotypisiation of mouse adipose tissue derived mesenchymal cells

Research paper thumbnail of Uporedni Pregled Sredstava Za Retrakciju Gingive

Acta medica medianae, 2013

In order to obtain an adequate impression of demarcation line area of a prepared tooth located in... more In order to obtain an adequate impression of demarcation line area of a prepared tooth located in or below the gingival edge it is necessary to perform the dilatation and drying of the gingival sulcus using retraction cord impregnated with adequate retraction agents. The aim of the study was to carry out comparative analysis of advantages and disadvantages of commercially available gingival retraction agents. Commercial retraction agents include astringents (metal salts) and vasoconstrictors on the basis of epinephrine. Further research should be aimed at examining the possibility of using sympathomimetic vasoconstrictors (tetrahydrosolin and oxymetasolin) for gingival retraction. Acta Medica Medianae 2012; 51(1):81-84.

Research paper thumbnail of Comparative Review of Gingival Retraction Agents

Acta medica medianae, 2012

In order to obtain an adequate impression of demarcation line area of a prepared tooth located in... more In order to obtain an adequate impression of demarcation line area of a prepared tooth located in or below the gingival edge it is necessary to perform the dilatation and drying of the gingival sulcus using retraction cord impregnated with adequate retraction agents. The aim of the study was to carry out comparative analysis of advantages and disadvantages of commercially available gingival retraction agents. Commercial retraction agents include astringents (metal salts) and vasoconstrictors on the basis of epinephrine. Further research should be aimed at examining the possibility of using sympathomimetic vasoconstrictors (tetrahydrosolin and oxymetasolin) for gingival retraction. Acta Medica Medianae 2012; 51(1):81-84.

Research paper thumbnail of Addition of blood to a phycogenic bone substitute leads to increased in vivo vascularization

The present study aimed to analyze the effects of the addition of blood to the phycogenic bone su... more The present study aimed to analyze the effects of the addition of blood to the phycogenic bone substitute Algipore ® on the severity of in vivo tissue reaction. Initially, Fourier-transform infrared spectroscopy (FTIR) of the bone substitute was conducted to analyze its chemical composition. The subcutaneous implantation model in Balb/c mice was then applied for up to 30 d to analyze the tissue reactions on the basis of specialized histochemical, immunohistochemical, and histomorphometrical methods.

Research paper thumbnail of In vitro effects of Royal jelly on viability and proliferation of HeLa cells

Research paper thumbnail of Osteogenic potential of adipose-derived mesenchymal stem cells in the case of osteoporosis

Osteoporosis is a chronic progressive disease characterized by reduced bone mineral density and b... more Osteoporosis is a chronic progressive disease characterized by reduced bone mineral density and bone mass which leads to increased risk of developing bone fractures. Diseases that are commonly associated with osteoporosis, or can contribute to its development are type 2 diabetes mellitus and asthma. In this study we examined the potential of adipose-derived mesenchymal stem cells (ADSCs) to proliferate and to differentiate into osteoblasts in vitro from a female patient who is suffering from osteoporosis, type 2 diabetes mellitus and asthma, and who obtained bilateral subtrochanteric femur fractures. Subcutaneous adipose tissue close to the fracture was taken during the surgical procedure of osteosynthesis and mesenchymal stem cells were isolated by digestion of the tissue and subsequently cultivated in vitro. After the second passage proliferation assay was performed and cells were cultivated in an osteogenic differentiation medium for a period of 21 days. Using cytochemical and immunocytochemical methods we have shown that these cells have the potential to differentiate into osteoblasts. These results suggest that adipose-derived mesenchymal stem cells obtained from patients with aforementioned chronic diseases can be used in tissue engineering for reparation of bone defects.

Research paper thumbnail of The effect of macrophages on initiation of osteogenesis in subcutaneous implants

Research paper thumbnail of Spontaneous Chromosomal Instability Related to Aging and Gender

Research paper thumbnail of Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions

Research paper thumbnail of Osteogenic potential of freshly isolated cells of adipose-derived mesenchymal fraction applied with nanoparticles

The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tis... more The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.

Research paper thumbnail of 1-Poster PhysChem2014 FTIR SEM-EDX

Research paper thumbnail of Neovasculogenic and osteogenic potential of adipose-derived stem cells implanted in combination with bone mineral matrix and platelet-rich plasma at ectopic site

Research paper thumbnail of Osteo-regenerative potential of adipose tissue-derived mesenchymal stem cells

Research paper thumbnail of Adipose derived mesenchymal stem cells as a model for study osteoinductive activity of bone substituting biomaterials

Adipose derived mesenchymal stem cells (ADMSC) show remarkable plasticity because of their potent... more Adipose derived mesenchymal stem cells (ADMSC) show remarkable plasticity because of their potential for differentiating into various cell types, including cells which are characteristic for osteogenic process, such as osteoblasts and endothelial cells. Their cultivation on biomaterial scaffolds is a common approach in tissue engineering. In our previous in vivo studies we used ADMSC for implantation into the experimental animals onto the bone substituting biomaterials as carriers. Our in vitro studies showed that cultivation of human and murine ADMSC in normal and osteoinductive medium with or without biomaterials can be a good model for assessing their bone substituting potential.

Research paper thumbnail of Histochemical Analysis of In vivo Osteogenic Processes in Constructs Consisted of Adipose-Derived Stem Cells, Platelet-rich Plasma and Bone Mineral Matrix

Cell-based bone tissue engineering is considered as promising approach in bone defects healing. T... more Cell-based bone tissue engineering is considered as promising approach in bone defects healing. Three basic components in this approach are osteoprogenitor cells, sources of growth and proliferation factors and bone substitute material as carrier for previous two elements. Aim of this study was to evaluate in vivo osteogenic potential of Adipose-derived Stem Cells (ADSCs) delivered on bone mineral matrix granules with addition of platelet-rich plasma (PRP). ADSCs isolated from supra-epididymal fat pads of Balb/c mice were subjected to in vitro osteoinduction for fifteen days before constructs were prepared. Prepared constructs were implanted subcutaneously in Balb/c mice. Histochemistry analysis was performed on implants extracted two and eight weeks after implantation. According to obtained results, this approach could be of benefit for bone tissue engineering purposes. Keywords: bone mineral matrix, ADSC, bone tissue engineering, PRP, osteoinduction Acknowledgements: This work was...

Research paper thumbnail of Procena produkcije slobodnih radikala kiseonika u peritonealnim makrofagima miša semikvantitativnim mikroskopskim NBT testom

Research paper thumbnail of Examination of In vitro Neovasculogenic Potential of Mice Adipose-derived Stem Cells

Examination of In vitro Neovasculogenic Potential of Mice Adipose-derived Stem Cells Jelena Najda... more Examination of In vitro Neovasculogenic Potential of Mice Adipose-derived Stem Cells Jelena Najdanović1, Vladimir Cvetković2, Marija Vukelić-Nikolić1, Jelena Živković1, Sanja Stojanović1, Stevo Najman1 1Faculty of Medicine, University of Nis, Bul. dr Zorana Đinđića 81, Niš, Serbia 2Faculty of Sciences and Mathematics, University of Nis, Višegradska 33, Niš, Serbia Bone tissue regeneration depends on oxygen and essential nutrients supply which is mediated by blood vessels. Therefore, neovasculogenesis and angiogenesis are of great importance in bone tissue engineering. The aim of this study was to examine neovasculogenic potential of adipose-derived stem cells (ADSC) subjected to endothelogenesis in vitro. ADSCs were isolated from mice supraepididymal adipose tissue, expanded in proliferative media and subjected to endothelogenic differentiation for fifteen days. Relative expression levels of endothelial-related gene markers: VCAM, Flt-1 and Egr-1 were monitored applying quantitative...