Mutsuaki Suzuki | National Institute of Genetics (original) (raw)
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Papers by Mutsuaki Suzuki
Neuroscience Letters, Sep 1, 1993
ABSTRACT
Acta Histochemica, 2011
We conducted a study in which we demonstrated by means of immunoperoxidase and immunofluorescence... more We conducted a study in which we demonstrated by means of immunoperoxidase and immunofluorescence methods the presence of pituitary adenylate cyclase-activating peptide 38 (PACAP-38) immunoreactivity in the small intestine of adult New Hampshire chickens and its co-localization with VIP. In particular we describe for the first time the presence of PACAP-positive cells in the epithelium of crypts and villi. Using double immunostaining, we observed that these two peptides were widely co-localized in the nerve structures of duodenum and jejunum with the exception of the ileum, where we noticed a faint co-localization regarding the nerve fibers of the lamina propria of the villi. Furthermore, the two peptides were occasionally co-stored in the epithelial cells of the mucosa. Our findings suggest that in the chicken small intestine, PACAP can be considered, not only as a neuromodulator released by nerve elements, but also as a gut hormone secreted by endocrine cells, and it appears likely to have a role in the regulation of important intestinal physiological functions.
Biomedical Research-tokyo, 1994
HortScience
We previously reported that growth of lisianthus [Eustoma grandiflorum (Raf.) Shinn.] seedlings i... more We previously reported that growth of lisianthus [Eustoma grandiflorum (Raf.) Shinn.] seedlings is accelerated by amending the growing medium with 1% (w/w) chitosan. This finding prompted us to search for organic nitrogenous other substances like chitosan which could accelerate seedling growth. Seeds of E. grandiflorum `Peter blue line 2'were sown in a sandy loam growing medium containing 1% (w/w) chitosan, tryptone, casein, collagen or gelatin. At eleven weeks after sowing, leaf length and width, fresh and dry weights of the shoots and roots of twelve plants were determined for each treatment. Eleven weeks after sowing, the leaves at the fifth node had expanded in the chitosan, tryptone and collagen treatments while the leaves of the third node had not yet expanded in control plants. Fresh and dry weights of shoots and roots were significantly greater for plants grown in media amended with chitosan or tryptone. Percent nitrogen (N) and potassium (K) in the shoots and roots and ...
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Gastrointestinal Endocrinology, 1990
Biomedical Research, 2002
Substance P-induced [Ca2+], signaling in a single epithelial cell of isolated crypt from guinea p... more Substance P-induced [Ca2+], signaling in a single epithelial cell of isolated crypt from guinea pig distal colon was investigated by Ca" imaging tecluiique. Substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) induced a concentration-dependent transient increase in [Ca2+], with a rank order of potency of SPI>-NKA?-">>NKB. A NK1 receptor antagonist, FK888 (l'>l0"i M) significantly reduced the response of SP (IOT M). A phosphatidylinositol-specific phospholipase C (PI
Biomedical Research
In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri m... more In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri mdix) extracts having potent binding affinity to cholecystokinin type-B (CCK-B) receptor. Radio receptor assay for CCK-B receptor was successfully used for detection of the active components throughout the purification steps. Saiko root was extracted with distilled water, ethanol, acetone and hydrochloric acid, respectively. Among the extracts, the water extract exhibited the highest binding activity for CCK-B receptor in rat cerebral cortex and GH3 cells. The water extract was then used as the starting material. Isolation of the active component was performed by (1) gel filtration on Sephadex G-50, (2) ion exchange chromatography on a Q-Sepharose column, and (3) reverse phase HPLC on a YMC D-ODS column. Finally, the active fraction having a strong binding affinity for CCK-B receptor was purified by RP-HPLC to give a single sharp peak eluted at 17 min. These results suggest that the substance isolated in the present study may be a peptide. In fact, the sequence analysis of this substance revealed that the N-terminal sequence was Ser-Tyr-Arg-Glu-Pro-Asn-Thr-. Saiko (Bupleuri radix) is a well-known Chinese herbal medicine and extensively prescribed in the Chinese clinical practice. Traditionally, the pre
Biomedical Research
In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri m... more In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri mdix) extracts having potent binding affinity to cholecystokinin type-B (CCK-B) receptor. Radio receptor assay for CCK-B receptor was successfully used for detection of the active components throughout the purification steps. Saiko root was extracted with distilled water, ethanol, acetone and hydrochloric acid, respectively. Among the extracts, the water extract exhibited the highest binding activity for CCK-B receptor in rat cerebral cortex and GH3 cells. The water extract was then used as the starting material. Isolation of the active component was performed by (1) gel filtration on Sephadex G-50, (2) ion exchange chromatography on a Q-Sepharose column, and (3) reverse phase HPLC on a YMC D-ODS column. Finally, the active fraction having a strong binding affinity for CCK-B receptor was purified by RP-HPLC to give a single sharp peak eluted at 17 min. These results suggest that the substance isolated in the present study may be a peptide. In fact, the sequence analysis of this substance revealed that the N-terminal sequence was Ser-Tyr-Arg-Glu-Pro-Asn-Thr-. Saiko (Bupleuri radix) is a well-known Chinese herbal medicine and extensively prescribed in the Chinese clinical practice. Traditionally, the pre
Experimental and Toxicologic Pathology, 2004
In vitro biological studies on cigarette smoke have usually been made using either cigarette smok... more In vitro biological studies on cigarette smoke have usually been made using either cigarette smoke condensateobtained by trapping the particulate phase of smoke on a filter, or soluble smoke components -obtained by trapping cigarette smoke in buffer solution. However, these approaches may not truly reflect the physical and chemical condition of freshly generated smoke. Clearly it is important to be able to evaluate the biological effects of fresh smoke on mammalian cells for a better understanding of the potential effects of smoking. The CULTEX TM technology is a new experimental system for cultivation and exposure techniques enhanced the efficiency of in vitro studies, and allows direct exposure of cells intermittently at the air/liquid interface with ultrafine particles, gases, or mixtures of both which fixedly flows. The CULTEX TM technology has therefore been modified to evaluate the biological effects of whole cigarette smoke in an in vitro system.
Annals of The New York Academy of Sciences, 2000
... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publicat... more ... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publication History. Issue published online: 25 JAN 2006; Article first published online: 25 JAN 2006. SEARCH. ... 527589. 2 Reddix, R., A. Kuwahara, L. Wallace & HJ Cooke. 1994. ...
Annals of The New York Academy of Sciences, 1994
Annals of The New York Academy of Sciences, 2000
... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publicat... more ... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publication History. Issue published online: 25 JAN 2006; Article first published online: 25 JAN 2006. SEARCH. ... 527589. 2 Reddix, R., A. Kuwahara, L. Wallace & HJ Cooke. 1994. ...
Annals of The New York Academy of Sciences, 1994
Neuroscience Letters, Sep 1, 1993
ABSTRACT
Acta Histochemica, 2011
We conducted a study in which we demonstrated by means of immunoperoxidase and immunofluorescence... more We conducted a study in which we demonstrated by means of immunoperoxidase and immunofluorescence methods the presence of pituitary adenylate cyclase-activating peptide 38 (PACAP-38) immunoreactivity in the small intestine of adult New Hampshire chickens and its co-localization with VIP. In particular we describe for the first time the presence of PACAP-positive cells in the epithelium of crypts and villi. Using double immunostaining, we observed that these two peptides were widely co-localized in the nerve structures of duodenum and jejunum with the exception of the ileum, where we noticed a faint co-localization regarding the nerve fibers of the lamina propria of the villi. Furthermore, the two peptides were occasionally co-stored in the epithelial cells of the mucosa. Our findings suggest that in the chicken small intestine, PACAP can be considered, not only as a neuromodulator released by nerve elements, but also as a gut hormone secreted by endocrine cells, and it appears likely to have a role in the regulation of important intestinal physiological functions.
Biomedical Research-tokyo, 1994
HortScience
We previously reported that growth of lisianthus [Eustoma grandiflorum (Raf.) Shinn.] seedlings i... more We previously reported that growth of lisianthus [Eustoma grandiflorum (Raf.) Shinn.] seedlings is accelerated by amending the growing medium with 1% (w/w) chitosan. This finding prompted us to search for organic nitrogenous other substances like chitosan which could accelerate seedling growth. Seeds of E. grandiflorum `Peter blue line 2'were sown in a sandy loam growing medium containing 1% (w/w) chitosan, tryptone, casein, collagen or gelatin. At eleven weeks after sowing, leaf length and width, fresh and dry weights of the shoots and roots of twelve plants were determined for each treatment. Eleven weeks after sowing, the leaves at the fifth node had expanded in the chitosan, tryptone and collagen treatments while the leaves of the third node had not yet expanded in control plants. Fresh and dry weights of shoots and roots were significantly greater for plants grown in media amended with chitosan or tryptone. Percent nitrogen (N) and potassium (K) in the shoots and roots and ...
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Gastrointestinal Endocrinology, 1990
Biomedical Research, 2002
Substance P-induced [Ca2+], signaling in a single epithelial cell of isolated crypt from guinea p... more Substance P-induced [Ca2+], signaling in a single epithelial cell of isolated crypt from guinea pig distal colon was investigated by Ca" imaging tecluiique. Substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) induced a concentration-dependent transient increase in [Ca2+], with a rank order of potency of SPI>-NKA?-">>NKB. A NK1 receptor antagonist, FK888 (l'>l0"i M) significantly reduced the response of SP (IOT M). A phosphatidylinositol-specific phospholipase C (PI
Biomedical Research
In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri m... more In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri mdix) extracts having potent binding affinity to cholecystokinin type-B (CCK-B) receptor. Radio receptor assay for CCK-B receptor was successfully used for detection of the active components throughout the purification steps. Saiko root was extracted with distilled water, ethanol, acetone and hydrochloric acid, respectively. Among the extracts, the water extract exhibited the highest binding activity for CCK-B receptor in rat cerebral cortex and GH3 cells. The water extract was then used as the starting material. Isolation of the active component was performed by (1) gel filtration on Sephadex G-50, (2) ion exchange chromatography on a Q-Sepharose column, and (3) reverse phase HPLC on a YMC D-ODS column. Finally, the active fraction having a strong binding affinity for CCK-B receptor was purified by RP-HPLC to give a single sharp peak eluted at 17 min. These results suggest that the substance isolated in the present study may be a peptide. In fact, the sequence analysis of this substance revealed that the N-terminal sequence was Ser-Tyr-Arg-Glu-Pro-Asn-Thr-. Saiko (Bupleuri radix) is a well-known Chinese herbal medicine and extensively prescribed in the Chinese clinical practice. Traditionally, the pre
Biomedical Research
In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri m... more In the present study, we have isolated and purified chemical components in Saiko root (Bupleuri mdix) extracts having potent binding affinity to cholecystokinin type-B (CCK-B) receptor. Radio receptor assay for CCK-B receptor was successfully used for detection of the active components throughout the purification steps. Saiko root was extracted with distilled water, ethanol, acetone and hydrochloric acid, respectively. Among the extracts, the water extract exhibited the highest binding activity for CCK-B receptor in rat cerebral cortex and GH3 cells. The water extract was then used as the starting material. Isolation of the active component was performed by (1) gel filtration on Sephadex G-50, (2) ion exchange chromatography on a Q-Sepharose column, and (3) reverse phase HPLC on a YMC D-ODS column. Finally, the active fraction having a strong binding affinity for CCK-B receptor was purified by RP-HPLC to give a single sharp peak eluted at 17 min. These results suggest that the substance isolated in the present study may be a peptide. In fact, the sequence analysis of this substance revealed that the N-terminal sequence was Ser-Tyr-Arg-Glu-Pro-Asn-Thr-. Saiko (Bupleuri radix) is a well-known Chinese herbal medicine and extensively prescribed in the Chinese clinical practice. Traditionally, the pre
Experimental and Toxicologic Pathology, 2004
In vitro biological studies on cigarette smoke have usually been made using either cigarette smok... more In vitro biological studies on cigarette smoke have usually been made using either cigarette smoke condensateobtained by trapping the particulate phase of smoke on a filter, or soluble smoke components -obtained by trapping cigarette smoke in buffer solution. However, these approaches may not truly reflect the physical and chemical condition of freshly generated smoke. Clearly it is important to be able to evaluate the biological effects of fresh smoke on mammalian cells for a better understanding of the potential effects of smoking. The CULTEX TM technology is a new experimental system for cultivation and exposure techniques enhanced the efficiency of in vitro studies, and allows direct exposure of cells intermittently at the air/liquid interface with ultrafine particles, gases, or mixtures of both which fixedly flows. The CULTEX TM technology has therefore been modified to evaluate the biological effects of whole cigarette smoke in an in vitro system.
Annals of The New York Academy of Sciences, 2000
... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publicat... more ... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publication History. Issue published online: 25 JAN 2006; Article first published online: 25 JAN 2006. SEARCH. ... 527589. 2 Reddix, R., A. Kuwahara, L. Wallace & HJ Cooke. 1994. ...
Annals of The New York Academy of Sciences, 1994
Annals of The New York Academy of Sciences, 2000
... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publicat... more ... Voice: +81542645794. suzukim@sea.u-shizuoka-ken.ac.jp or suzukim@mba.nifty-ne.jp. Publication History. Issue published online: 25 JAN 2006; Article first published online: 25 JAN 2006. SEARCH. ... 527589. 2 Reddix, R., A. Kuwahara, L. Wallace & HJ Cooke. 1994. ...
Annals of The New York Academy of Sciences, 1994