MUSTAPHA UMAR | Nigerian Institute of Leather and Science Technology Samaru-Zaria (original) (raw)

Papers by MUSTAPHA UMAR

Research paper thumbnail of Seroprevalence of Syphilis and Hepatitis C Virus Coinfection among Blood Donors Attending Aminu Kano Teaching Hospital, Kano-Nigeria

Research paper thumbnail of Comparative Analysis And Quality Assessment Of Treated And Untreated Tannery Effluents Discharged From NILEST Tannery, Zaria, Kaduna, Nigeria

An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery... more An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery was carried out with view to isolate and identify the bacterial species present in the tannery effluent, and to compare the level of microbial density of treated and untreated tannery effluents. Comparison between the level of contamination of the analyzed tannery effluent and the standard given by national and international environmental regulatory bodies was carried out. A total of 40 samples were collected; 20 each from treated and untreated samples. The samples were analyzed by multiple tube fermentation technique using Most Probable Number (MPN) method. All the untreated tannery effluent samples show the presence of coliform bacteria 40 (100%), while, 6 (30%) of the treated tannery effluent samples show the presence of coliform bacteria. There is significant difference (p<0.05) between microbial load in the treated tannery effluent and untreated effluents analyzed. Most of the t...

Research paper thumbnail of Screening of Locally Isolated Penicillium Species from the Soil for Amylase Production

Journal of Applied Life Sciences International, 2020

Background: A number of microorganisms are capable of degrading amylase and only a few of these m... more Background: A number of microorganisms are capable of degrading amylase and only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolyzing the amylase. Fungi are the main amylase-producing microorganisms. Aims: The present study targeted to screen Penicillium species isolated locally from soil for amylase production. Materials and Methods: In this research, fungal species belonging to genera penicillum species were isolated from soil and screened for their ability to degrade cellulose (lactose, fructose, sucrose and dextrose) for enzyme activity. Enzymatic fungi were evaluated after 7 days for the production of amylase enzymes by lactophenol microscopic examination. The concentrations for 7 days of production were formulated by dilution method as 0.148 mg/ml, 0.313 mg/ml, 0.303 mg/ml, 0.127 mg/ml, 0.161 mg/ml, 0.079 mg/ml and 0.099 mg/ml respectively. Results: The maximum amylase production was obtained on the 2nd day of production whi...

Research paper thumbnail of Seroprevalence of Syphilis and Hepatitis C Virus Coinfection among Blood Donors Attending Aminu Kano Teaching Hospital, Kano-Nigeria

Research paper thumbnail of Comparative Studies on the Prevalence of Salmonella Species in Two Homemade Fermented Beverages (Zobo And Kunun-Zaki) Sold At Samaru, Zaria, Kaduna, Nigeria

A survey of the comparative studies on the prevalence of Salmonella species in two home-made beve... more A survey of the comparative studies on the prevalence of Salmonella species in two home-made beverages sold at Samaru, Zaria was carried out. Ten (10) samples of freshly formulated Kunun-Zaki and Zobo were obtained from five (5) different sales locations in Samaru, Zaria, and analyzed using the standard plate counts, to determine their respective bacteriological quality.

Research paper thumbnail of Comparative Analysis And Quality Assessment Of Treated And Untreated Tannery Effluents Discharged From NILEST Tannery, Zaria, Kaduna, Nigeria

An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery... more An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery was carried out with view to isolate and identify the bacterial species present in the tannery effluent, and to compare the level of microbial density of treated and untreated tannery effluents. Comparison between the level of contamination of the analyzed tannery effluent and the standard given by national and international environmental regulatory bodies was carried out. A total of 40 samples were collected; 20 each from treated and untreated samples. The samples were analyzed by multiple tube fermentation technique using Most Probable Number (MPN) method. All the untreated tannery effluent samples show the presence of coliform bacteria 40 (100%), while, 6 (30%) of the treated tannery effluent samples show the presence of coliform bacteria. There is significant difference (p<0.05) between microbial load in the treated tannery effluent and untreated effluents analyzed. Most of the t...

Research paper thumbnail of Bacteriological Quality Assessment and Antibiogram Profile of Bacteria Associated with Sachet Drinking Water Sold at Zaria, Northern Nigeria

International Journal of Pathogen Research, 2019

Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sach... more Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sachet drinking water was carried out with view to determine the bacterial load and fitness of the water sold in the study area for human consumption. Materials and Methods: Ten samples of different brands were aseptically collected and analyzed using heterotrophic count and most probable number technique. The isolated bacteria were microscopically and biochemically characterized and finally confirmed using Box™ Easygel® E. coli Quantitube™ Test Kit (Micrology Laboratories manufactures, Easygel®, USA) biotyping. Physicochemical analysis of the samples was done using standard methods. The isolated bacteria were screened for antimicrobial susceptibility using Kirby-Bauer disc diffusion technique. Results: The total heterotrophic bacterial count ranged from 1.0×102 to 3.0×102 cfu/ml, with MPN/100ml values ranged from <0.03 to 1.2. The temperature ranged from 8 to 18°C, at the pH range of 5.0...

Research paper thumbnail of Screening of Locally Isolated Penicillium Species from the Soil for Amylase Production

Journal of Applied Life Sciences International, 2020

Background: A number of microorganisms are capable of degrading amylase and only a few of these m... more Background: A number of microorganisms are capable of degrading amylase and only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolyzing the amylase. Fungi are the main amylase-producing microorganisms. Aims: The present study targeted to screen Penicillium species isolated locally from soil for amylase production. Materials and Methods: In this research, fungal species belonging to genera penicillum species were isolated from soil and screened for their ability to degrade cellulose (lactose, fructose, sucrose and dextrose) for enzyme activity. Enzymatic fungi were evaluated after 7 days for the production of amylase enzymes by lactophenol microscopic examination. The concentrations for 7 days of production were formulated by dilution method as 0.148 mg/ml, 0.313 mg/ml, 0.303 mg/ml, 0.127 mg/ml, 0.161 mg/ml, 0.079 mg/ml and 0.099 mg/ml respectively. Results: The maximum amylase production was obtained on the 2nd day of production whi...

Research paper thumbnail of Comparative Studies on the Prevalence of Salmonella Species in Two Homemade Fermented Beverages (Zobo And Kunun-Zaki) Sold At Samaru, Zaria, Kaduna, Nigeria

A survey of the comparative studies on the prevalence of Salmonella species in two home-made beve... more A survey of the comparative studies on the prevalence of Salmonella species in two home-made beverages sold at Samaru, Zaria was carried out. Ten (10) samples of freshly formulated Kunun-Zaki and Zobo were obtained from five (5) different sales locations in Samaru, Zaria, and analyzed using the standard plate counts, to determine their respective bacteriological quality.

Research paper thumbnail of Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

South Asian Journal of Research in Microbiology, 2018

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lo... more A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cu...

Research paper thumbnail of Bacteriological Quality Assessment and Antibiogram Profile of Bacteria Associated with Sachet Drinking Water Sold at Zaria, Northern Nigeria

International Journal of Pathogen Research, 2019

Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sach... more Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sachet drinking water was carried out with view to determine the bacterial load and fitness of the water sold in the study area for human consumption. Materials and Methods: Ten samples of different brands were aseptically collected and analyzed using heterotrophic count and most probable number technique. The isolated bacteria were microscopically and biochemically characterized and finally confirmed using Box™ Easygel® E. coli Quantitube™ Test Kit (Micrology Laboratories manufactures, Easygel®, USA) biotyping. Physicochemical analysis of the samples was done using standard methods. The isolated bacteria were screened for antimicrobial susceptibility using Kirby-Bauer disc diffusion technique. Results: The total heterotrophic bacterial count ranged from 1.0×102 to 3.0×102 cfu/ml, with MPN/100ml values ranged from <0.03 to 1.2. The temperature ranged from 8 to 18°C, at the pH range of 5.0...

Research paper thumbnail of Evaluation of Phytochemical, Antimicrobial Activities and Toxicological Analysis of Scent Leaf (Ocimum gratissimum L.) Leaf Extracts

Asian Journal of Research in Medical and Pharmaceutical Sciences, 2019

Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of th... more Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of the leaves of scent leaf (Ocimum gratissimum L.) were investigated. Methods: All the analyses were carried out using standard scientific procedures using Soxhlet extraction, well-diffusion agar antimicrobial testing and in vivo acute toxicity testing. Results: The phytochemical analysis according to standard screening tests using conventional protocols revealed the presence of anthraquinone, saponins, tannins, terpenoids and alkaloids, which were detected in methanol extract analyzed. But, flavonoids, glycosides, phlobatannins and steroids were not detected in the methanol extracts analyzed. While only flavonoids were detected in chloroform extract. All other phytochemicals were absent. The extract fractions generally exhibited slight antibacterial activities on Staphylococcus aureus, Bacillus subtilis, Salmonella Typhi and Escherichia coli. But, the extracts showed no effect against Candi...

Research paper thumbnail of Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

South Asian Journal of Research in Microbiology, 2018

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lo... more A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cu...

Research paper thumbnail of Seroprevalence of Treponema pallidum Infection among Cohorts of Pregnant Women Attending a Selected Hospital at Northern Nigeria

International STD Research & Reviews, 2019

Background: Syphilis is a multifaceted disease with serious implications for the pregnant women a... more Background: Syphilis is a multifaceted disease with serious implications for the pregnant women and the foetus. Treponema pallidum, the causative agent of syphilis has been a public health challenge for centuries. Sexually transmitted infections (STIs) among pregnant women are wide spread in the developing countries, and constitute a major public health problem in sub-Saharan Africa. Information regarding the prevalence of syphilis in pregnant women is scanty from the north-west zone of Nigeria. Aims: Evaluation of seroprevalence of Treponema pallidum infection among pregnant women attending a selected hospital at Northern Nigeria was carried out, with view to assess the socio-demographic data and predisposing factors of syphilis among the study population. Methods: Exactly 200 pregnant women, who attended antenatal clinic of the selected hospital at Northern Nigeria, from July to September, 2015 were screened for syphilis using syphilis rapid immunochromatographic test for in-vitro...

Research paper thumbnail of Evaluation of Phytochemical, Antimicrobial Activities and Toxicological Analysis of Scent Leaf (Ocimum gratissimum L.) Leaf Extracts

Asian Journal of Research in Medical and Pharmaceutical Sciences, 2019

Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of th... more Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of the leaves of scent leaf (Ocimum gratissimum L.) were investigated. Methods: All the analyses were carried out using standard scientific procedures using Soxhlet extraction, well-diffusion agar antimicrobial testing and in vivo acute toxicity testing. Results: The phytochemical analysis according to standard screening tests using conventional protocols revealed the presence of anthraquinone, saponins, tannins, terpenoids and alkaloids, which were detected in methanol extract analyzed. But, flavonoids, glycosides, phlobatannins and steroids were not detected in the methanol extracts analyzed. While only flavonoids were detected in chloroform extract. All other phytochemicals were absent. The extract fractions generally exhibited slight antibacterial activities on Staphylococcus aureus, Bacillus subtilis, Salmonella Typhi and Escherichia coli. But, the extracts showed no effect against Candi...

Research paper thumbnail of Seroprevalence of Treponema pallidum Infection among Cohorts of Pregnant Women Attending a Selected Hospital at Northern Nigeria

International STD Research & Reviews, 2019

Background: Syphilis is a multifaceted disease with serious implications for the pregnant women a... more Background: Syphilis is a multifaceted disease with serious implications for the pregnant women and the foetus. Treponema pallidum, the causative agent of syphilis has been a public health challenge for centuries. Sexually transmitted infections (STIs) among pregnant women are wide spread in the developing countries, and constitute a major public health problem in sub-Saharan Africa. Information regarding the prevalence of syphilis in pregnant women is scanty from the north-west zone of Nigeria. Aims: Evaluation of seroprevalence of Treponema pallidum infection among pregnant women attending a selected hospital at Northern Nigeria was carried out, with view to assess the socio-demographic data and predisposing factors of syphilis among the study population. Methods: Exactly 200 pregnant women, who attended antenatal clinic of the selected hospital at Northern Nigeria, from July to September, 2015 were screened for syphilis using syphilis rapid immunochromatographic test for in-vitro...

Research paper thumbnail of Antibiotics Susceptibility Study of Uropathogens Isolated from Asymptomatic Pregnant Women Attending a Tertiary Maternity Clinic in Northern Nigeria

Journal of Complementary and Alternative Medical Research, 2017

Research paper thumbnail of Antibiotics Susceptibility Study of Uropathogens Isolated from Asymptomatic Pregnant Women Attending a Tertiary Maternity Clinic in Northern Nigeria

Journal of Complementary and Alternative Medical Research, 2017

Research paper thumbnail of Prevalence of Bacterial, Trichomonas and Candidal Vaginosis among Females in Angwan-Fulani, Palladan in Zaria, Nigeria

Journal of Applied Life Sciences International, 2016

Research paper thumbnail of Characterization and antimicrobial susceptibility pattern of clinical isolates of Enterococci at a tertiary care hospital in Mumbai, India

Annals of Tropical Medicine and Public Health, 2012

Research paper thumbnail of Seroprevalence of Syphilis and Hepatitis C Virus Coinfection among Blood Donors Attending Aminu Kano Teaching Hospital, Kano-Nigeria

Research paper thumbnail of Comparative Analysis And Quality Assessment Of Treated And Untreated Tannery Effluents Discharged From NILEST Tannery, Zaria, Kaduna, Nigeria

An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery... more An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery was carried out with view to isolate and identify the bacterial species present in the tannery effluent, and to compare the level of microbial density of treated and untreated tannery effluents. Comparison between the level of contamination of the analyzed tannery effluent and the standard given by national and international environmental regulatory bodies was carried out. A total of 40 samples were collected; 20 each from treated and untreated samples. The samples were analyzed by multiple tube fermentation technique using Most Probable Number (MPN) method. All the untreated tannery effluent samples show the presence of coliform bacteria 40 (100%), while, 6 (30%) of the treated tannery effluent samples show the presence of coliform bacteria. There is significant difference (p<0.05) between microbial load in the treated tannery effluent and untreated effluents analyzed. Most of the t...

Research paper thumbnail of Screening of Locally Isolated Penicillium Species from the Soil for Amylase Production

Journal of Applied Life Sciences International, 2020

Background: A number of microorganisms are capable of degrading amylase and only a few of these m... more Background: A number of microorganisms are capable of degrading amylase and only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolyzing the amylase. Fungi are the main amylase-producing microorganisms. Aims: The present study targeted to screen Penicillium species isolated locally from soil for amylase production. Materials and Methods: In this research, fungal species belonging to genera penicillum species were isolated from soil and screened for their ability to degrade cellulose (lactose, fructose, sucrose and dextrose) for enzyme activity. Enzymatic fungi were evaluated after 7 days for the production of amylase enzymes by lactophenol microscopic examination. The concentrations for 7 days of production were formulated by dilution method as 0.148 mg/ml, 0.313 mg/ml, 0.303 mg/ml, 0.127 mg/ml, 0.161 mg/ml, 0.079 mg/ml and 0.099 mg/ml respectively. Results: The maximum amylase production was obtained on the 2nd day of production whi...

Research paper thumbnail of Seroprevalence of Syphilis and Hepatitis C Virus Coinfection among Blood Donors Attending Aminu Kano Teaching Hospital, Kano-Nigeria

Research paper thumbnail of Comparative Studies on the Prevalence of Salmonella Species in Two Homemade Fermented Beverages (Zobo And Kunun-Zaki) Sold At Samaru, Zaria, Kaduna, Nigeria

A survey of the comparative studies on the prevalence of Salmonella species in two home-made beve... more A survey of the comparative studies on the prevalence of Salmonella species in two home-made beverages sold at Samaru, Zaria was carried out. Ten (10) samples of freshly formulated Kunun-Zaki and Zobo were obtained from five (5) different sales locations in Samaru, Zaria, and analyzed using the standard plate counts, to determine their respective bacteriological quality.

Research paper thumbnail of Comparative Analysis And Quality Assessment Of Treated And Untreated Tannery Effluents Discharged From NILEST Tannery, Zaria, Kaduna, Nigeria

An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery... more An investigation on bacteriological quality assessment of effluent discharged from NILEST tannery was carried out with view to isolate and identify the bacterial species present in the tannery effluent, and to compare the level of microbial density of treated and untreated tannery effluents. Comparison between the level of contamination of the analyzed tannery effluent and the standard given by national and international environmental regulatory bodies was carried out. A total of 40 samples were collected; 20 each from treated and untreated samples. The samples were analyzed by multiple tube fermentation technique using Most Probable Number (MPN) method. All the untreated tannery effluent samples show the presence of coliform bacteria 40 (100%), while, 6 (30%) of the treated tannery effluent samples show the presence of coliform bacteria. There is significant difference (p<0.05) between microbial load in the treated tannery effluent and untreated effluents analyzed. Most of the t...

Research paper thumbnail of Bacteriological Quality Assessment and Antibiogram Profile of Bacteria Associated with Sachet Drinking Water Sold at Zaria, Northern Nigeria

International Journal of Pathogen Research, 2019

Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sach... more Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sachet drinking water was carried out with view to determine the bacterial load and fitness of the water sold in the study area for human consumption. Materials and Methods: Ten samples of different brands were aseptically collected and analyzed using heterotrophic count and most probable number technique. The isolated bacteria were microscopically and biochemically characterized and finally confirmed using Box™ Easygel® E. coli Quantitube™ Test Kit (Micrology Laboratories manufactures, Easygel®, USA) biotyping. Physicochemical analysis of the samples was done using standard methods. The isolated bacteria were screened for antimicrobial susceptibility using Kirby-Bauer disc diffusion technique. Results: The total heterotrophic bacterial count ranged from 1.0×102 to 3.0×102 cfu/ml, with MPN/100ml values ranged from <0.03 to 1.2. The temperature ranged from 8 to 18°C, at the pH range of 5.0...

Research paper thumbnail of Screening of Locally Isolated Penicillium Species from the Soil for Amylase Production

Journal of Applied Life Sciences International, 2020

Background: A number of microorganisms are capable of degrading amylase and only a few of these m... more Background: A number of microorganisms are capable of degrading amylase and only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolyzing the amylase. Fungi are the main amylase-producing microorganisms. Aims: The present study targeted to screen Penicillium species isolated locally from soil for amylase production. Materials and Methods: In this research, fungal species belonging to genera penicillum species were isolated from soil and screened for their ability to degrade cellulose (lactose, fructose, sucrose and dextrose) for enzyme activity. Enzymatic fungi were evaluated after 7 days for the production of amylase enzymes by lactophenol microscopic examination. The concentrations for 7 days of production were formulated by dilution method as 0.148 mg/ml, 0.313 mg/ml, 0.303 mg/ml, 0.127 mg/ml, 0.161 mg/ml, 0.079 mg/ml and 0.099 mg/ml respectively. Results: The maximum amylase production was obtained on the 2nd day of production whi...

Research paper thumbnail of Comparative Studies on the Prevalence of Salmonella Species in Two Homemade Fermented Beverages (Zobo And Kunun-Zaki) Sold At Samaru, Zaria, Kaduna, Nigeria

A survey of the comparative studies on the prevalence of Salmonella species in two home-made beve... more A survey of the comparative studies on the prevalence of Salmonella species in two home-made beverages sold at Samaru, Zaria was carried out. Ten (10) samples of freshly formulated Kunun-Zaki and Zobo were obtained from five (5) different sales locations in Samaru, Zaria, and analyzed using the standard plate counts, to determine their respective bacteriological quality.

Research paper thumbnail of Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

South Asian Journal of Research in Microbiology, 2018

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lo... more A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cu...

Research paper thumbnail of Bacteriological Quality Assessment and Antibiogram Profile of Bacteria Associated with Sachet Drinking Water Sold at Zaria, Northern Nigeria

International Journal of Pathogen Research, 2019

Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sach... more Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sachet drinking water was carried out with view to determine the bacterial load and fitness of the water sold in the study area for human consumption. Materials and Methods: Ten samples of different brands were aseptically collected and analyzed using heterotrophic count and most probable number technique. The isolated bacteria were microscopically and biochemically characterized and finally confirmed using Box™ Easygel® E. coli Quantitube™ Test Kit (Micrology Laboratories manufactures, Easygel®, USA) biotyping. Physicochemical analysis of the samples was done using standard methods. The isolated bacteria were screened for antimicrobial susceptibility using Kirby-Bauer disc diffusion technique. Results: The total heterotrophic bacterial count ranged from 1.0×102 to 3.0×102 cfu/ml, with MPN/100ml values ranged from <0.03 to 1.2. The temperature ranged from 8 to 18°C, at the pH range of 5.0...

Research paper thumbnail of Evaluation of Phytochemical, Antimicrobial Activities and Toxicological Analysis of Scent Leaf (Ocimum gratissimum L.) Leaf Extracts

Asian Journal of Research in Medical and Pharmaceutical Sciences, 2019

Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of th... more Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of the leaves of scent leaf (Ocimum gratissimum L.) were investigated. Methods: All the analyses were carried out using standard scientific procedures using Soxhlet extraction, well-diffusion agar antimicrobial testing and in vivo acute toxicity testing. Results: The phytochemical analysis according to standard screening tests using conventional protocols revealed the presence of anthraquinone, saponins, tannins, terpenoids and alkaloids, which were detected in methanol extract analyzed. But, flavonoids, glycosides, phlobatannins and steroids were not detected in the methanol extracts analyzed. While only flavonoids were detected in chloroform extract. All other phytochemicals were absent. The extract fractions generally exhibited slight antibacterial activities on Staphylococcus aureus, Bacillus subtilis, Salmonella Typhi and Escherichia coli. But, the extracts showed no effect against Candi...

Research paper thumbnail of Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

South Asian Journal of Research in Microbiology, 2018

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lo... more A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cu...

Research paper thumbnail of Seroprevalence of Treponema pallidum Infection among Cohorts of Pregnant Women Attending a Selected Hospital at Northern Nigeria

International STD Research & Reviews, 2019

Background: Syphilis is a multifaceted disease with serious implications for the pregnant women a... more Background: Syphilis is a multifaceted disease with serious implications for the pregnant women and the foetus. Treponema pallidum, the causative agent of syphilis has been a public health challenge for centuries. Sexually transmitted infections (STIs) among pregnant women are wide spread in the developing countries, and constitute a major public health problem in sub-Saharan Africa. Information regarding the prevalence of syphilis in pregnant women is scanty from the north-west zone of Nigeria. Aims: Evaluation of seroprevalence of Treponema pallidum infection among pregnant women attending a selected hospital at Northern Nigeria was carried out, with view to assess the socio-demographic data and predisposing factors of syphilis among the study population. Methods: Exactly 200 pregnant women, who attended antenatal clinic of the selected hospital at Northern Nigeria, from July to September, 2015 were screened for syphilis using syphilis rapid immunochromatographic test for in-vitro...

Research paper thumbnail of Evaluation of Phytochemical, Antimicrobial Activities and Toxicological Analysis of Scent Leaf (Ocimum gratissimum L.) Leaf Extracts

Asian Journal of Research in Medical and Pharmaceutical Sciences, 2019

Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of th... more Aim: The phytochemical screening, antibacterial activities and in vivo toxicity of extracts of the leaves of scent leaf (Ocimum gratissimum L.) were investigated. Methods: All the analyses were carried out using standard scientific procedures using Soxhlet extraction, well-diffusion agar antimicrobial testing and in vivo acute toxicity testing. Results: The phytochemical analysis according to standard screening tests using conventional protocols revealed the presence of anthraquinone, saponins, tannins, terpenoids and alkaloids, which were detected in methanol extract analyzed. But, flavonoids, glycosides, phlobatannins and steroids were not detected in the methanol extracts analyzed. While only flavonoids were detected in chloroform extract. All other phytochemicals were absent. The extract fractions generally exhibited slight antibacterial activities on Staphylococcus aureus, Bacillus subtilis, Salmonella Typhi and Escherichia coli. But, the extracts showed no effect against Candi...

Research paper thumbnail of Seroprevalence of Treponema pallidum Infection among Cohorts of Pregnant Women Attending a Selected Hospital at Northern Nigeria

International STD Research & Reviews, 2019

Background: Syphilis is a multifaceted disease with serious implications for the pregnant women a... more Background: Syphilis is a multifaceted disease with serious implications for the pregnant women and the foetus. Treponema pallidum, the causative agent of syphilis has been a public health challenge for centuries. Sexually transmitted infections (STIs) among pregnant women are wide spread in the developing countries, and constitute a major public health problem in sub-Saharan Africa. Information regarding the prevalence of syphilis in pregnant women is scanty from the north-west zone of Nigeria. Aims: Evaluation of seroprevalence of Treponema pallidum infection among pregnant women attending a selected hospital at Northern Nigeria was carried out, with view to assess the socio-demographic data and predisposing factors of syphilis among the study population. Methods: Exactly 200 pregnant women, who attended antenatal clinic of the selected hospital at Northern Nigeria, from July to September, 2015 were screened for syphilis using syphilis rapid immunochromatographic test for in-vitro...

Research paper thumbnail of Antibiotics Susceptibility Study of Uropathogens Isolated from Asymptomatic Pregnant Women Attending a Tertiary Maternity Clinic in Northern Nigeria

Journal of Complementary and Alternative Medical Research, 2017

Research paper thumbnail of Antibiotics Susceptibility Study of Uropathogens Isolated from Asymptomatic Pregnant Women Attending a Tertiary Maternity Clinic in Northern Nigeria

Journal of Complementary and Alternative Medical Research, 2017

Research paper thumbnail of Prevalence of Bacterial, Trichomonas and Candidal Vaginosis among Females in Angwan-Fulani, Palladan in Zaria, Nigeria

Journal of Applied Life Sciences International, 2016

Research paper thumbnail of Characterization and antimicrobial susceptibility pattern of clinical isolates of Enterococci at a tertiary care hospital in Mumbai, India

Annals of Tropical Medicine and Public Health, 2012

Research paper thumbnail of Thermodynamics Study of Congo Red Dye Adsorption onto H 2 SO 4 Treated Rice Husk (Oryza sativa) Powder

Dutse Journal of Pure and Applied Sciences, 2020

Dyes are hazardous, mutagenic and carcinogenic organic pollutants discharged into the environment... more Dyes are hazardous, mutagenic and carcinogenic organic pollutants discharged into the environment from textile effluents, tanneries and other industries. Rice husk was believed to serve as good sorbent for many metals and basic dyes including Congo red dye. The present study targeted to remove Congo red dye from a prepared wastewater using rice husk as adsorbent, with view to use thermodynamic parameters such as entropy, enthalpy and free energy models to explain the mechanism for the dye adsorption using rice husk powder as adsorbent. In this research, rice husk was collected from Panshekara area, Kano state, Nigeria. The sample was processed using standard techniques. The sample was treated with sulphuric acid and used for the uptake of the dye by adsorption process. The filtered samples were developed using UV spectrometry. Thermodynamic studies were carried out using standard procedures. Congo red dye can be removed from aqueous medium by the process of adsorption using rice husk adsorbent. The thermodynamic studies of the adsorption process that were conducted using various temperatures (30, 35, 40 and 45°C) revealed that the reaction is endothermic, and there was increase in the randomness of the solid/solution interface during the adsorption of Congo red onto the rice husk. The thermodynamic parameters analyzed such as enthalpy (ΔH), entropy (ΔS), free energy (ΔG) and isosteric heat of adsorption (ΔHr), suggest that the overall adsorption process was physisorption. The enthalpy-entropy compensation theory can be applied to the sorption phenomena of rice, since sorption and desorption processes can be controlled by enthalpy.