Ramu Adela | NIPER (National Institute of Pharmaceutical Education and Research) (original) (raw)

Papers by Ramu Adela

Clinical Drug Investigation, Nov 4, 2022

Journal of diabetes research, Oct 19, 2021

Background. Diabetes mellitus (DM) is a chronic metabolic disorder associated with higher risk of... more Background. Diabetes mellitus (DM) is a chronic metabolic disorder associated with higher risk of having cardiovascular disease. Platelets play a promising role in the pathogenesis of cardiovascular complications in diabetes. Since last several decades, garlic and its bioactive components are extensively studied in diabetes and its complications. Our aim was to explore the antiplatelet property of allyl methyl sulfide (AMS) focusing on ameliorating platelet activation in diabetes. Method. We used streptozotocin-(STZ-) induced diabetic rats as model for type 1 diabetes. We have evaluated the effect of allyl methyl sulfide on platelet activation by administrating AMS to diabetic rats for 10 weeks. Flow cytometry-based analysis was used to evaluate the platelet activation, platelet aggregation, platelet macrophage interaction, and endogenous ROS generation in the platelets obtained from control, diabetes, and AMS-and aspirin-treated diabetic rats. Results. AMS treatment for 10 weeks effectively reduced the blood glucose levels in diabetic rats. Three weeks of AMS (50 mg/kg/day) treatment did not reduce the activation of platelets but a significant (p < 0:05) decrease was observed after 10 weeks of treatment. Oral administration of AMS significantly (p < 0:05) reduced the baseline and also reduced ADP-induced aggregation of platelets after 3 and 10 weeks of treatment. Furthermore, 10 weeks of AMS treatment in diabetic rats attenuated the endogenous ROS content (p < 0:05) of platelets and platelet macrophage interactions. The inhibition of platelet activation in diabetic rats after AMS treatment was comparable with aspirin treatment (30 mg/kg/day). Conclusion. We observed an inhibitory effect of allyl methyl sulfide on platelet aggregation, platelet activation, platelet macrophage interaction, and increased ROS levels in type 1 diabetes. Our data suggests that AMS can be useful to control cardiovascular complication in diabetes via inhibition of platelet activation.

Phytotherapy Research, Jan 14, 2023

Several preclinical studies have focused on the beneficial effects of garlic on cardiovascular di... more Several preclinical studies have focused on the beneficial effects of garlic on cardiovascular diseases, but the results were inconsistent. We performed a systematic review and meta‐analysis on the effect of garlic powder tablets and aged garlic extract (AGE) in CAD patients, mainly focusing on blood pressure, coronary artery calcification, lipid profile, and inflammatory markers. We searched PubMed, Cochrane CENTRAL, and Google Scholar to identify randomized controlled trials which examined garlic's effect on CAD patients. The standardized mean difference with 95% CI was calculated using fixed‐effect or random‐effect models. Garlic has shown statistically significant changes of HDL (SMD = 0.18; 95% CI = −0.00 to 0.37; p = .05); LDL (SMD = −0.27; 95% CI = −0.46 to −0.08; p = .004), apolipoprotein‐A (SMD = 0.68; 95% CI = 0.24 1.13; p = .002), C‐RP (SMD = −0.59; 95% CI = −0.92 to −0.25; p = .0007), IL‐6 (SMD = −1.08; 95% CI = −2.17 to 0.01; p = .05), homocysteine (SMD = −0.66; 95% CI = −1.04 to −0.28; p = .0007) and CAC score (SMD = −1.61; 95% CI = −2.66 to −0.57; p = .003). In the case of subgroup analysis, the overall effect was significantly effective in reducing TC, LDL levels and improving HDL levels in CV risk patients. Our study findings provide consistent evidence that intake of garlic reduces CVD risk factors. However, garlic could be considered a safe natural medicine to debilitate inflammation in CAD patients.

Clinical Drug Investigation, Jan 12, 2023

Phytotherapy Research

Clinical Drug Investigation

Environmental Science and Pollution Research

Oxidative Medicine and Cellular Longevity

Objectives. Transition from cardiac hypertrophy to failure involves adverse metabolic reprogrammi... more Objectives. Transition from cardiac hypertrophy to failure involves adverse metabolic reprogramming involving mitochondrial dysfunction. We have earlier shown that vitamin D deficiency induces heart failure, at least in part, through insulin resistance. However, whether activation of vitamin D receptor (VDR) can attenuate heart failure and underlying metabolic phenotype requires investigation. Thus, we aimed to assess the cardioprotective potential of paricalcitol, a vitamin D receptor-activator, against cardiac hypertrophy and failure in high-fat high-fructose-fed rats. Methods. Male Sprague Dawley rats were fed control (Con) or high-fat high-fructose (HFHFrD) diet for 20 weeks. After 12 weeks, rats from HFHFrD group were divided into the following: HFHFrD, HFHFrD+P (paricalcitol i.p. 0.08 μg/kg/day) and HFHFrD+E (enalapril maleate i.p. 10 mg/kg/day). Intraperitoneal glucose tolerance test, blood pressure measurement, and 2D echocardiography were performed. Cardiac fibrosis was ass...

Frontiers in Immunology

BackgroundRecently, our group identified increased platelet-mediated inflammation in type 2 diabe... more BackgroundRecently, our group identified increased platelet-mediated inflammation in type 2 diabetes (T2DM) patients, and it is a well-established risk factor for diabetes complications, particularly for the development of cardiovascular diseases (CVD). Furthermore, vitamin D is reported to play an important role in the modulation of platelet hyperactivity and immune function, although the effect of vitamin D on platelet-mediated inflammation is not well studied. Hence, we aimed to investigate the effect of vitamin D supplementation on platelet-mediated inflammation in T2DM patients.MethodsAfter screening a total of 201 subjects, our randomized, double-blind, placebo-controlled trial included 59 vitamin-D-deficient T2DM subjects, and the participants were randomly assigned to placebo (n = 29) or vitamin D3 (n = 30) for 6 months. Serum vitamin D metabolite levels, immunome profiling, platelet activation, and platelet–immune cell aggregate formation were measured at baseline and at th...

Human Nutrition & Metabolism, 2022

Chemosphere, 2022

Limited information is available about the levels of exposure of paraben and bisphenols emerging ... more Limited information is available about the levels of exposure of paraben and bisphenols emerging from PCPs use in Indian women and the risk associated with it. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine the concentrations of six parabens (methyl-, ethyl-, propyl-, butyl, benzyl-, and heptyl-parabens) and 8 bisphenols (Bisphenol A, B, F, P, S, Z, AP, and AF) in PCPs samples (n = 114) obtained from Indian market and in the urine samples of young adult females (n = 52). The concentrations measured in PCPs and urine samples were used to determine the estimated daily intake. The mean concentrations of 6 parabens and 8 bisphenols in PCPs ranged from 38.3 to 2.38 × 105 ng/mL and 2.71-148 ng/mL, respectively. In urine samples analysed, the mean concentrations of 6 parabens and 8 bisphenols ranged from 0.007 to 293 ng/mL and 0.10-10.77 ng/mL, respectively. There was no significant correlation of EDCs with age, BMI and waist-to-hip ratio (WHR), but significant correlations (p < 0.05) were observed between urinary paraben and bisphenol concentrations. A statistically significant difference (p < 0.05) exists between the BMI and WHR groups by bisphenol concentrations. Estimated daily intake and exposure risks for parabens and bisphenols revealed no possible concerns for Indian young adult females. Hitherto, this is the first study to show that Indian young adult females were exposed to parabens and bisphenols. This study provides evidence on PCPs usage contribute to the urinary concentrations of EDCs.

Additional file 1. KEGG pathway analysis sheet.

International Journal of Pharmacy Practice, 2022

Objectives Hepcidin is linked to glucose metabolism in women with gestational diabetes mellitus (... more Objectives Hepcidin is linked to glucose metabolism in women with gestational diabetes mellitus (GDM). This systematic review and meta-analysis was conducted to determine the association between hepcidin levels and GDM. A literature search was performed using different databases to identify potential studies investigating hepcidin association in GDM patients. The effect sizes were calculated based on the standardized mean difference (SMD) and Fisher’s Z value with a 95% confidence interval (CI). Key findings Out of 827 articles, only 7 case–control studies satisfied the inclusion and exclusion criteria. The pooled SMD of circulatory hepcidin levels in GDM patients was considerably higher than normal pregnant women (SMD = 1.69; 95% CI, 0.86 to 2.53; P < 0.0001). This study also observed that hepcidin levels were positively correlated with ferritin levels (r = 0.264; Z = 0.27; P < 0.0001). Furthermore, a subgroup analysis of serum and plasma groups revealed significantly higher ...

Inflammation plays pivotal role in the pathogenesis of diabetes and cardiovascular diseases.Seden... more Inflammation plays pivotal role in the pathogenesis of diabetes and cardiovascular diseases.Sedentary life style, environmental factors and obesity are responsible for increasing the prevalence of diabetes and cardiovascular diseases.Multiple mechanisms like glucotoxicity and lipotoxicity, oxidative stress and endoplasmicreticulum stress are involved in the pathogenesis of diabetes and cardiovascular diseases. In the present article, we haveexplained about the pathogenesis of inflammation and the role of inflammatory markers in diabetes and cardiovascular diseases. Although there are several drugs to reduce these disease burdens, we need more efficient drugs to reduce the disease progression by targeting inflammatory pathway. More experimental and clinical studies are required to introduce intervention therapies to reduce the inflammation process in cardiovascular diseases. Here, in this review we have explained the inflammatory pathways and inflammatory biomarkers that are essentia...

PLOS ONE, 2015

Background We have previously reported that increased glucose levels were associated with higher ... more Background We have previously reported that increased glucose levels were associated with higher serum nitric oxide (NO) levels in fructose-fed insulin resistant rats. However, the relationship between hyperglycemia and serum NO level was not clear. Therefore, the present study was designed to find the association between hyperglycemia and serum NO levels in Type 2 diabetic (T2DM) patients and T2DM with cardiovascular complication.

Canadian Journal of Physiology and Pharmacology, 2013

Garlic is widely recognized for its immense therapeutic potential. Garlic has been shown to exert... more Garlic is widely recognized for its immense therapeutic potential. Garlic has been shown to exert its beneficial effects against a wide spectrum of diseases, including cancer, diabetes, and microbial infections, as well as immunological and cardiovascular disorders. Most of the research on garlic has indicated that garlic and its active compounds are effective in reducing cardiovascular and metabolic risk by normalizing abnormal plasma lipids, oxidized low density lipoproteins, abnormal platelet aggregation, high blood pressure, and cardiac injury. Some of the beneficial effects of dietary garlic against cardiovascular disorders are mediated via the generation of hydrogen sulfide and nitric oxide in cardiomyocytes and endothelial cells. Garlic has the potential to protect the heart against myocardial infarction, doxorubicin-induced cardiotoxicity, arrhythmia, hypertrophy, and ischemia–reperfusion injury. The induction of cardiac endogenous antioxidants and the reduction of lipid per...

RPS Pharmacy and Pharmacology Reports

Objectives Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are... more Objectives Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are the major public health issues. Though the prevalence of the disease is high, there is no approved pharmacological treatment. Obeticholic acid (OCA) has not been well described in terms of its efficacy and safety in NAFLD/NASH patients. Hence, we investigated the efficacy and safety of OCA in NAFLD/NASH patients. Methods PubMed, Cochrane CENTRAL, and Google Scholar were searched from 2001 to date for identifying randomized controlled trials which examined the OCA effect on liver enzymes, lipoproteins, and liver histology in NASH/NAFLD patients. Results Four relevant RCTs were identified and included for quantitative analysis. OCA exhibited significant reduction in ALT, AST and GGT levels, whereas, in case of liver histology, significant improvement in steatosis, hepatocellular ballooning, lobular inflammation and fibrosis was observed in OCA treatment group [RR: 1.25, 95% CI: (1.03 to 1...

License, which permits unrestricted use, distribution, and reproduction in any medium, provided t... more License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Growth differentiation factor-15 (GDF-15) is a stress responsive cytokine. It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. IncreasedGDF-15 level is linked with the progression and prognosis of the disease condition. Age, smoking, and environmental factors are other risk factors that may increase GDF-15 level. Most of the scientific studies reported that GDF-15 plays a protective role in different tissues. However, few ...