Shamkant B Badgujar | NIRRH (original) (raw)
Papers by Shamkant B Badgujar
Advances in Plant Sciences, 2009
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Pharmaceutical Biology, Jul 14, 2014
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Journal of Chromatography B
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Research Journal of Pharmacology and Pharmacodynamics, 2009
Wound healing activity of E uphorbia nivulia Buch.-Ham. (Euphorbiaceae) was evaluated using excis... more Wound healing activity of E uphorbia nivulia Buch.-Ham. (Euphorbiaceae) was evaluated using excision wound model in albino mice by applying latex topically on the area of excised wound once a day for 23 days. Plant latex significantly enhanced the rate of wound contraction and period of epithelialization, which is comparable to Soframycin. Latex of this plant significantly reduces the bleeding and whole blood clotting time. Phytochemical analysis revealed the presence of alkaloids, cynogenic glycosides, phenolics, tannins and saponins. The latex of Euphorbia nivulia possesses natural ingredients capable of arresting wound bleeding and accelerating wound healing properties which suggests healing potential to cure wound.
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152-160<span style="font-size:11.0pt;font-family: " times="" new="&qu... more 152-160<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (<i style="mso-bidi-font-style: normal">Ruie), Carica papaya<span style="mso-bidi-font-style: italic"> Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F. carica, <i style="mso-bidi-font-style: normal">E. nivulia and C. procera. The crude enzyme of Sabar, Ruie, Papaya and Anji...
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Attribution License, which permits unrestricted use, distribution, and reproduction in any medium... more Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A new cysteine protease named Nivulian-II has been purified from the latex of Euphorbia nivulia Buch.-Ham. The apparent molecular mass of Nivulian-II is 43670.846 Da (MALDI TOF/MS). Peptide mass fingerprint analysis revealed peptide matches to Maturase K (Q52ZV1 9MAGN) of Banksia quercifolia. The N-terminal sequence (DFPPNTCCCICC) showed partial homology with those of other cysteine proteinases of biological origin. This is the first paper to characterize a Nivulian-II of E. nivulia latex with respect to amino acid sequencing. 1.
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To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in ha... more To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was analyzed for caseinolytic activity, milk clotting activity, gelatinolytic activity and peroxidase activity. Highest caseinolytic activity was noted in latex of Pedilanthus
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Ethnomedicinal survey was conducted in hilly area of North Maharashtra, India. Till today Bhills,... more Ethnomedicinal survey was conducted in hilly area of North Maharashtra, India. Till today Bhills, Gavits, Kokanis, Mavachis, Padvis, Tadvis, Valvis and Vasaves tribes are exclusively dependent on forest. They have own system of herbal medicine. Information on 19 plant species of 18 angiosperm families which are traditionally used as medicine to cure Kavil (Jaundice) was collected. The plant part used, traditional preparation and doses for administration are given in the present paper.
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License, which permits unrestricted use, distribution, and reproduction in any medium, provided t... more License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Foeniculum vulgare Mill commonly called fennel has been used in traditional medicine for a wide range of ailments related to digestive, endocrine, reproductive, and respiratory systems. Additionally, it is also used as a galactagogue agent for lactating mothers. The review aims to gather the fragmented information available in the literature regarding morphology, ethnomedicinal applications, phytochemistry, pharmacology, and toxicology of Foeniculum vulgare. It also compiles available scientific evidence for the ethnobotanical claims and to identify gaps required to be filled by future research. Findings based on their traditional uses and scientific evaluation indicates that Foeniculum vulgare remains to be the most widely used herbal plant. It has been used for more than forty types of disorders. Phytochemical studies have shown the presence of numer...
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Ethnobotanical Leaflets, 2008
Analyses were carried out on latex obtained from twenty one plant species belonging to Khandesh r... more Analyses were carried out on latex obtained from twenty one plant species belonging to Khandesh region of Maharashtra, India, for the presence of possible secondary metabolites, namely alkaloids, flavonoids, terpenoids, cynogenic glycosides, phenolics, tannins and saponins. Phenolic compounds were found in all latex samples except in Ipomoea carnea Jacq. Seventy six percent species of laticiferous plants contain alkaloid. Terpenoids were found in the latex of Carica papaya L. and Manilkara zapota (L.) P. van Royen only however, remaining plants were devoid. Cynogenic glycosides and tannins were detected in 52 % and 48 % of latex samples respectively. Flavonoids and saponins were detected in equal number of latex samples i.e. 38 %. Out of this, four species of Apocynaceae and single species of Euphorbiaceae, Moraceae, Carricaceae, Convolvulaceae etc. showed flavonoid. While three species of Euphorbiaceae, two species of Apocynaceae and Moraceae and single species of Asclepiadaceae sh...
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Journal of Pharmacy Research, 2009
To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in ha... more To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was analyzed for caseinolytic activity, milk clotting activity, gelatinolytic activity and peroxidase activity. Highest caseinolytic activity was noted in latex of Pedilanthus tithymaloides (L.) Poit., followed by Ficus carica L., Calotropis gigantea (L.) R. Br. and Carica papaya L. Moderate caseinolytic activity was seen in Euphorbia milii Desmoul., Ficus hispida L.f. and Calotropis procera (Ait.) R. Br., and remaining plants possess less activity. Milk clotting activity was found highest in stem latex of Euphorbia nivulia L., followed by Carica papaya L., Ficus carica L., Calotropis procera (Ait.) R. Br., and Tabernaemontana divaricata (L.) R. Br. Six plants showed moderate milk clotting activity, however, such activity was not noticed in remaining six pl...
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BioTechnology: An Indian Journal, 2013
Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to b... more Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to be Bacillus subtilis. Studies on submerged fermentation revealed that maximum level i.e. 321 µg/ml/min of protease production was observed during early stationary phase. Optimization of fermentation mediumfor characterization of protease was carried out. Optimization parameter includes incubation period, temperature, pH, substrate concentration, carbon sources, nitrogen sources and tween 80 concentrations. The optimized conditions found for protease production were 400C at pH 10 (alkaline condition), with 10%inoculums, 2 ml tween 80, 1%sucrose, 1%ammoniumchloride and 20% casein, after 72 h of incubation stimulates protease production. The protease profile of the selected isolate shows its potential for its industrial applications.
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Natural Products: An Indian Journal, 2013
A new cysteine protease named nivulian was partially characterized from the latex of Euphorbia ni... more A new cysteine protease named nivulian was partially characterized from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family. It had good stability in the presence of local detergents viz., Tide®, Ujalla®, Nirma® and also Triton X-100 and Tween-80 at 370C. The protein stains (blood and egg yolk) were removed within 10 ÂÂ 15 min from the test fabric (cotton) cloth by the treatment of this enzyme. Thus this application of plant origin ÂÂnivulianÂÂ demonstrates feasibility for inclusion in laundry detergent formulation.
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Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The h... more Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for the treatment and management of different ailments. Euphorbia nivulia Buch. - Ham. a member of Euphorbiaceae family is a wild, thorny, xerophytic, succulent plant, commonly used in fencing of the agricultural field and also in dry barren areas. It has different biological activities for the treatment of several ailments of human being. It possesses antimicrobial, wound healing, haemostatic, larvicidal, insecticidal, nematicidal and cytotoxic activity. Chemically, it contains terpenes, glycoproteins, phytoelements and phytochemicals. This article provides informative data on ethnobotanical importance and bioprospecting of Euphorbia nivulia Buch. - Ham.
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Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. ... more Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F. carica, E. nivulia and C. procera. The crude enzyme of Sabar, Ruie, Papaya and Anjir latices exhibit high proteolytic activity with in a pH range from 6.0-7.8, 6.5-8.0, 6.0-7.5 and 6.0-7.0, while maximum milk clotting activity with in a pH range from 6.0-6.5, 6.0-7.0, 6.0-6.5 and 6.5-7.0, respectively. The optimum temperature of proteolytic enzymes of plant latices were recorded over the temperature range 10-80°C at pH 7.0. Whereas, the optimum temperature range of milk clotting protease(s) for milk coagulation was found in between 45-55°C. Proteolytic activity of all latices was inhibited b...
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Protease plays an important role in the management of defense mechanism of living organisms. This... more Protease plays an important role in the management of defense mechanism of living organisms. This review presents a comprehensive and exhaustive account of plant, animal and microbial enzymes with special reference to proteolytic properties. A large number of biological sources of proteases described in this review clearly demonstrate the importance of plants and microbial proteases in applied and industrial uses. Proteases have potential applications in food, dairy, detergent, leather, alcoholic beverages, brewing, meat, pharmaceutical and photographic industries. The research work on protease has been going on since seven decades but no exhaustive review on protease from last eleven years is available in the literature. This review attempts to focus on some of the difficulties observed in the earlier work and tried to find out possible solutions and bridging up this gap by introducing recent information regarding proteinases. This review provides information on diverse groups of p...
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An ethnobotanical study was conducted from January 2006 to October 2008 to investigate the uses o... more An ethnobotanical study was conducted from January 2006 to October 2008 to investigate the uses of medicinal plants for oral health care by different aborigines, such as Bhills, Gavits, Kokanis, Mavachis, Valvis, Pawras, Koknas and Vasaves, in the Nandurbar district of Maharashtra, India. Data were collected by interviewing native people, mainly elderly – engaged in farming and stock rising activities, housewives and local traditional medicinemen of different villages. The investigation revealed that a total 20 claims were obtained as distributed in 18 genera belonging to 14 families to treat various diseases and disorders of oral cavity, particularly in tooth decay. Information about local names, plant parts and different form of preparation used were recorded and are focused in given issue. In this study most commonly used family was Euphorbiaceae followed by Moraceae, Anacardiaceae, Fabaceae, Asteraceae, Acanthaceae, Meliaceae, Asclepiadaceae, Poaceae, Lythraceae, Sapotaceae, Cuc...
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A newlatex protein named nivulian-I of plant origin has been isolated and purified from the latex... more A newlatex protein named nivulian-I of plant origin has been isolated and purified from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family; by using centrifugation, acetone precipitation and ion exchange chromatography. This protein was characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis and mass spectroscopic analysis. Molecular mass of nivulian-I is 31486.985 Da. Peptide mass fingerprint revealed that, it fairlymatches with Tubulin alpha-1 chain (TBA1_ELEIN) of Eleusine indica (L.) Gaertn, a member of Poaceae family. This investigation infers that nivulian-I may be a novel protein of plant origin.
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Journal of Chromatography B
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Advances in Plant Sciences, 2009
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Pharmaceutical Biology, Jul 14, 2014
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Journal of Chromatography B
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Research Journal of Pharmacology and Pharmacodynamics, 2009
Wound healing activity of E uphorbia nivulia Buch.-Ham. (Euphorbiaceae) was evaluated using excis... more Wound healing activity of E uphorbia nivulia Buch.-Ham. (Euphorbiaceae) was evaluated using excision wound model in albino mice by applying latex topically on the area of excised wound once a day for 23 days. Plant latex significantly enhanced the rate of wound contraction and period of epithelialization, which is comparable to Soframycin. Latex of this plant significantly reduces the bleeding and whole blood clotting time. Phytochemical analysis revealed the presence of alkaloids, cynogenic glycosides, phenolics, tannins and saponins. The latex of Euphorbia nivulia possesses natural ingredients capable of arresting wound bleeding and accelerating wound healing properties which suggests healing potential to cure wound.
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152-160<span style="font-size:11.0pt;font-family: " times="" new="&qu... more 152-160<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (<i style="mso-bidi-font-style: normal">Ruie), Carica papaya<span style="mso-bidi-font-style: italic"> Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F. carica, <i style="mso-bidi-font-style: normal">E. nivulia and C. procera. The crude enzyme of Sabar, Ruie, Papaya and Anji...
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Attribution License, which permits unrestricted use, distribution, and reproduction in any medium... more Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A new cysteine protease named Nivulian-II has been purified from the latex of Euphorbia nivulia Buch.-Ham. The apparent molecular mass of Nivulian-II is 43670.846 Da (MALDI TOF/MS). Peptide mass fingerprint analysis revealed peptide matches to Maturase K (Q52ZV1 9MAGN) of Banksia quercifolia. The N-terminal sequence (DFPPNTCCCICC) showed partial homology with those of other cysteine proteinases of biological origin. This is the first paper to characterize a Nivulian-II of E. nivulia latex with respect to amino acid sequencing. 1.
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To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in ha... more To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was analyzed for caseinolytic activity, milk clotting activity, gelatinolytic activity and peroxidase activity. Highest caseinolytic activity was noted in latex of Pedilanthus
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Ethnomedicinal survey was conducted in hilly area of North Maharashtra, India. Till today Bhills,... more Ethnomedicinal survey was conducted in hilly area of North Maharashtra, India. Till today Bhills, Gavits, Kokanis, Mavachis, Padvis, Tadvis, Valvis and Vasaves tribes are exclusively dependent on forest. They have own system of herbal medicine. Information on 19 plant species of 18 angiosperm families which are traditionally used as medicine to cure Kavil (Jaundice) was collected. The plant part used, traditional preparation and doses for administration are given in the present paper.
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License, which permits unrestricted use, distribution, and reproduction in any medium, provided t... more License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Foeniculum vulgare Mill commonly called fennel has been used in traditional medicine for a wide range of ailments related to digestive, endocrine, reproductive, and respiratory systems. Additionally, it is also used as a galactagogue agent for lactating mothers. The review aims to gather the fragmented information available in the literature regarding morphology, ethnomedicinal applications, phytochemistry, pharmacology, and toxicology of Foeniculum vulgare. It also compiles available scientific evidence for the ethnobotanical claims and to identify gaps required to be filled by future research. Findings based on their traditional uses and scientific evaluation indicates that Foeniculum vulgare remains to be the most widely used herbal plant. It has been used for more than forty types of disorders. Phytochemical studies have shown the presence of numer...
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Ethnobotanical Leaflets, 2008
Analyses were carried out on latex obtained from twenty one plant species belonging to Khandesh r... more Analyses were carried out on latex obtained from twenty one plant species belonging to Khandesh region of Maharashtra, India, for the presence of possible secondary metabolites, namely alkaloids, flavonoids, terpenoids, cynogenic glycosides, phenolics, tannins and saponins. Phenolic compounds were found in all latex samples except in Ipomoea carnea Jacq. Seventy six percent species of laticiferous plants contain alkaloid. Terpenoids were found in the latex of Carica papaya L. and Manilkara zapota (L.) P. van Royen only however, remaining plants were devoid. Cynogenic glycosides and tannins were detected in 52 % and 48 % of latex samples respectively. Flavonoids and saponins were detected in equal number of latex samples i.e. 38 %. Out of this, four species of Apocynaceae and single species of Euphorbiaceae, Moraceae, Carricaceae, Convolvulaceae etc. showed flavonoid. While three species of Euphorbiaceae, two species of Apocynaceae and Moraceae and single species of Asclepiadaceae sh...
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Journal of Pharmacy Research, 2009
To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in ha... more To identify the potent source of protease that can be used as ideal milk clotting enzyme(s) in handmade dairy products of latex obtained from twenty one plant species belonging to Northern (Khandesh) region of Maharashtra, India, was analyzed for caseinolytic activity, milk clotting activity, gelatinolytic activity and peroxidase activity. Highest caseinolytic activity was noted in latex of Pedilanthus tithymaloides (L.) Poit., followed by Ficus carica L., Calotropis gigantea (L.) R. Br. and Carica papaya L. Moderate caseinolytic activity was seen in Euphorbia milii Desmoul., Ficus hispida L.f. and Calotropis procera (Ait.) R. Br., and remaining plants possess less activity. Milk clotting activity was found highest in stem latex of Euphorbia nivulia L., followed by Carica papaya L., Ficus carica L., Calotropis procera (Ait.) R. Br., and Tabernaemontana divaricata (L.) R. Br. Six plants showed moderate milk clotting activity, however, such activity was not noticed in remaining six pl...
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BioTechnology: An Indian Journal, 2013
Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to b... more Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to be Bacillus subtilis. Studies on submerged fermentation revealed that maximum level i.e. 321 µg/ml/min of protease production was observed during early stationary phase. Optimization of fermentation mediumfor characterization of protease was carried out. Optimization parameter includes incubation period, temperature, pH, substrate concentration, carbon sources, nitrogen sources and tween 80 concentrations. The optimized conditions found for protease production were 400C at pH 10 (alkaline condition), with 10%inoculums, 2 ml tween 80, 1%sucrose, 1%ammoniumchloride and 20% casein, after 72 h of incubation stimulates protease production. The protease profile of the selected isolate shows its potential for its industrial applications.
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Natural Products: An Indian Journal, 2013
A new cysteine protease named nivulian was partially characterized from the latex of Euphorbia ni... more A new cysteine protease named nivulian was partially characterized from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family. It had good stability in the presence of local detergents viz., Tide®, Ujalla®, Nirma® and also Triton X-100 and Tween-80 at 370C. The protein stains (blood and egg yolk) were removed within 10 ÂÂ 15 min from the test fabric (cotton) cloth by the treatment of this enzyme. Thus this application of plant origin ÂÂnivulianÂÂ demonstrates feasibility for inclusion in laundry detergent formulation.
Bookmarks Related papers MentionsView impact
Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The h... more Nature always stands as a golden mark to exemplify the outstanding phenomenon of symbiosis. The history of herbal medicines is as old as human civilization. Many herbal remedies have been employed in various medicinal systems for the treatment and management of different ailments. Euphorbia nivulia Buch. - Ham. a member of Euphorbiaceae family is a wild, thorny, xerophytic, succulent plant, commonly used in fencing of the agricultural field and also in dry barren areas. It has different biological activities for the treatment of several ailments of human being. It possesses antimicrobial, wound healing, haemostatic, larvicidal, insecticidal, nematicidal and cytotoxic activity. Chemically, it contains terpenes, glycoproteins, phytoelements and phytochemicals. This article provides informative data on ethnobotanical importance and bioprospecting of Euphorbia nivulia Buch. - Ham.
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Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. ... more Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F. carica, E. nivulia and C. procera. The crude enzyme of Sabar, Ruie, Papaya and Anjir latices exhibit high proteolytic activity with in a pH range from 6.0-7.8, 6.5-8.0, 6.0-7.5 and 6.0-7.0, while maximum milk clotting activity with in a pH range from 6.0-6.5, 6.0-7.0, 6.0-6.5 and 6.5-7.0, respectively. The optimum temperature of proteolytic enzymes of plant latices were recorded over the temperature range 10-80°C at pH 7.0. Whereas, the optimum temperature range of milk clotting protease(s) for milk coagulation was found in between 45-55°C. Proteolytic activity of all latices was inhibited b...
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Protease plays an important role in the management of defense mechanism of living organisms. This... more Protease plays an important role in the management of defense mechanism of living organisms. This review presents a comprehensive and exhaustive account of plant, animal and microbial enzymes with special reference to proteolytic properties. A large number of biological sources of proteases described in this review clearly demonstrate the importance of plants and microbial proteases in applied and industrial uses. Proteases have potential applications in food, dairy, detergent, leather, alcoholic beverages, brewing, meat, pharmaceutical and photographic industries. The research work on protease has been going on since seven decades but no exhaustive review on protease from last eleven years is available in the literature. This review attempts to focus on some of the difficulties observed in the earlier work and tried to find out possible solutions and bridging up this gap by introducing recent information regarding proteinases. This review provides information on diverse groups of p...
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An ethnobotanical study was conducted from January 2006 to October 2008 to investigate the uses o... more An ethnobotanical study was conducted from January 2006 to October 2008 to investigate the uses of medicinal plants for oral health care by different aborigines, such as Bhills, Gavits, Kokanis, Mavachis, Valvis, Pawras, Koknas and Vasaves, in the Nandurbar district of Maharashtra, India. Data were collected by interviewing native people, mainly elderly – engaged in farming and stock rising activities, housewives and local traditional medicinemen of different villages. The investigation revealed that a total 20 claims were obtained as distributed in 18 genera belonging to 14 families to treat various diseases and disorders of oral cavity, particularly in tooth decay. Information about local names, plant parts and different form of preparation used were recorded and are focused in given issue. In this study most commonly used family was Euphorbiaceae followed by Moraceae, Anacardiaceae, Fabaceae, Asteraceae, Acanthaceae, Meliaceae, Asclepiadaceae, Poaceae, Lythraceae, Sapotaceae, Cuc...
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A newlatex protein named nivulian-I of plant origin has been isolated and purified from the latex... more A newlatex protein named nivulian-I of plant origin has been isolated and purified from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family; by using centrifugation, acetone precipitation and ion exchange chromatography. This protein was characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis and mass spectroscopic analysis. Molecular mass of nivulian-I is 31486.985 Da. Peptide mass fingerprint revealed that, it fairlymatches with Tubulin alpha-1 chain (TBA1_ELEIN) of Eleusine indica (L.) Gaertn, a member of Poaceae family. This investigation infers that nivulian-I may be a novel protein of plant origin.
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Journal of Chromatography B
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