PRATIK KUMAR JAGTAP | NIT RAIPUR (original) (raw)
Papers by PRATIK KUMAR JAGTAP
Nanoscience & Nanotechnology-Asia, 2020
Background: The synthesis of metal Nanoparticles is a growing area of research interest due to it... more Background: The synthesis of metal Nanoparticles is a growing area of research interest due to its potential in the applications and development of advanced technologies. Here we have stressed on the Facile green synthesis approach that connects the nanotechnology and biotechnology. Methods: The method involves use of biological reducing agent cum stabilizing agent (capping agent). A comparative account of particle dimension and surface properties of the synthesized nano particles using Broccoli (Brassicaoleracea var.italica) extract is also presented. Results: The reduction process used in the synthesis was simple and convenient to handle and monitored by UV--Vis spectroscopy showing the absorbance maxima of various samples at 322nm, 496nm and 536nm using different solvents. The presence of active proteins and phenolic groups present in biomass before and after reduction was identified by FTIR. Conclusion: The crystalline morphology and size of the Nanoparticles were examined by TE...
International Journal for Research in Applied Science and Engineering Technology, 2019
Nanoparticles are widely used in biotechnology and biomedical fields such as in diagnosis and the... more Nanoparticles are widely used in biotechnology and biomedical fields such as in diagnosis and therapy. Recently, synthesizing metal nanoparticles using plants has been extensively studied and has been recognized as a green and efficient way for further exploiting plant parts as convenient nanofactories. The importance of nanotechnology is highly increased in last years. Gold and silver nanoparticles are significant in the process due to their many peculiarities such as surface Plasmon absorption and the surface accessibility for further functionalization. As gold and silver nanoparticle are proved to be safest for drugs applications they are considered very important and used in several applications. Synthesis of gold and silver nanoparticle can be done through three methods like Physical, Chemical reduction and Biological or Green methods. Present work have been presented to focus on the green method of its synthesis. The biosynthesis of nanoparticles has many advantages over tedious, toxic and expensive physical and chemical methods of synthesis. The goal, was to develop a reliable, ecofriendly and easy process for the synthesis of gold and silver nanoparticles. The size and shape of nanoparticle are the key to their biomedical property. Green synthesis of nanoparticle is feasible way for the future and this review provides gold and silver nanoparticle synthesis by green method because in recent years' efficient green chemistry methods for the synthesis of metal nanoparticle has become major focus of researchers.
Bioanalysis, 2018
A simple, sensitive and robust technique of hollow-fiber drop-to-drop solvent microextraction cou... more A simple, sensitive and robust technique of hollow-fiber drop-to-drop solvent microextraction coupled with GC-MS has been successfully developed for the detection of antidepressant drug nortriptyline in human blood and urine samples. The recoveries of the drug from the spiked samples are found to be well within the range and appropriate to support the method. The LOD for the drug was obtained to be 0.007, 0.009 and 0.021 μg ml in deionized water, urine and blood samples of human subjects, respectively. Linearity was obtained over the concentration range of 0.5-5.0 mg l in deionized water with correlation coefficient 0.99672.
Indian Journal of Pharmaceutical Education and Research, 2018
A novel analytical technique of dynamic drop-to-drop solvent microextraction (DDSME) is developed... more A novel analytical technique of dynamic drop-to-drop solvent microextraction (DDSME) is developed to determine phenothiazine drug promethazine commonly used in cough syrups and in aesthetic drinks, purple drank most common among youths. Here we apply coupling of highly sensitive technique of GC-MS with dynamic DDSME for the subnanomolar detection of promethazine drug from human biological samples (blood and urine). The method is a virtually solvent free and miniaturized version of traditional Liquidliquid microextraction (LLME), where the solvent to aqueous ratio is greatly reduced. The best optimum parameters for the dynamic DDSME technique were: extraction time of 10 m, using 1-Octanol as the extraction organic solvent, volume of extraction solvent 3 µl, volume of donar aqueous phase 20 µl and with no salt addition, the calibration range for dynamic DDSME is from 0.0 to 5.0 µg ml-1 with correlation coefficient (R 2) of >0.984. LOD for the drug was found to be 0.007 µg/ ml.
Nanoscience & Nanotechnology-Asia, 2020
Background: The synthesis of metal Nanoparticles is a growing area of research interest due to it... more Background: The synthesis of metal Nanoparticles is a growing area of research interest due to its potential in the applications and development of advanced technologies. Here we have stressed on the Facile green synthesis approach that connects the nanotechnology and biotechnology. Methods: The method involves use of biological reducing agent cum stabilizing agent (capping agent). A comparative account of particle dimension and surface properties of the synthesized nano particles using Broccoli (Brassicaoleracea var.italica) extract is also presented. Results: The reduction process used in the synthesis was simple and convenient to handle and monitored by UV--Vis spectroscopy showing the absorbance maxima of various samples at 322nm, 496nm and 536nm using different solvents. The presence of active proteins and phenolic groups present in biomass before and after reduction was identified by FTIR. Conclusion: The crystalline morphology and size of the Nanoparticles were examined by TE...
International Journal for Research in Applied Science and Engineering Technology, 2019
Nanoparticles are widely used in biotechnology and biomedical fields such as in diagnosis and the... more Nanoparticles are widely used in biotechnology and biomedical fields such as in diagnosis and therapy. Recently, synthesizing metal nanoparticles using plants has been extensively studied and has been recognized as a green and efficient way for further exploiting plant parts as convenient nanofactories. The importance of nanotechnology is highly increased in last years. Gold and silver nanoparticles are significant in the process due to their many peculiarities such as surface Plasmon absorption and the surface accessibility for further functionalization. As gold and silver nanoparticle are proved to be safest for drugs applications they are considered very important and used in several applications. Synthesis of gold and silver nanoparticle can be done through three methods like Physical, Chemical reduction and Biological or Green methods. Present work have been presented to focus on the green method of its synthesis. The biosynthesis of nanoparticles has many advantages over tedious, toxic and expensive physical and chemical methods of synthesis. The goal, was to develop a reliable, ecofriendly and easy process for the synthesis of gold and silver nanoparticles. The size and shape of nanoparticle are the key to their biomedical property. Green synthesis of nanoparticle is feasible way for the future and this review provides gold and silver nanoparticle synthesis by green method because in recent years' efficient green chemistry methods for the synthesis of metal nanoparticle has become major focus of researchers.
Bioanalysis, 2018
A simple, sensitive and robust technique of hollow-fiber drop-to-drop solvent microextraction cou... more A simple, sensitive and robust technique of hollow-fiber drop-to-drop solvent microextraction coupled with GC-MS has been successfully developed for the detection of antidepressant drug nortriptyline in human blood and urine samples. The recoveries of the drug from the spiked samples are found to be well within the range and appropriate to support the method. The LOD for the drug was obtained to be 0.007, 0.009 and 0.021 μg ml in deionized water, urine and blood samples of human subjects, respectively. Linearity was obtained over the concentration range of 0.5-5.0 mg l in deionized water with correlation coefficient 0.99672.
Indian Journal of Pharmaceutical Education and Research, 2018
A novel analytical technique of dynamic drop-to-drop solvent microextraction (DDSME) is developed... more A novel analytical technique of dynamic drop-to-drop solvent microextraction (DDSME) is developed to determine phenothiazine drug promethazine commonly used in cough syrups and in aesthetic drinks, purple drank most common among youths. Here we apply coupling of highly sensitive technique of GC-MS with dynamic DDSME for the subnanomolar detection of promethazine drug from human biological samples (blood and urine). The method is a virtually solvent free and miniaturized version of traditional Liquidliquid microextraction (LLME), where the solvent to aqueous ratio is greatly reduced. The best optimum parameters for the dynamic DDSME technique were: extraction time of 10 m, using 1-Octanol as the extraction organic solvent, volume of extraction solvent 3 µl, volume of donar aqueous phase 20 µl and with no salt addition, the calibration range for dynamic DDSME is from 0.0 to 5.0 µg ml-1 with correlation coefficient (R 2) of >0.984. LOD for the drug was found to be 0.007 µg/ ml.