Kunal Shukal | NMIMS University (original) (raw)
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Papers by Kunal Shukal
Biological chemistry, Jan 1, 2006
We have recently reported that a GC-rich palindromic repeat sequence presumably adopts a stable f... more We have recently reported that a GC-rich palindromic repeat sequence presumably adopts a stable fold-back tetraplex DNA structure under supercoiling. To establish the biological significance of this structure, we inserted this sequence between two direct repeat sequences, separated by 200 bp, in a plasmid. We then investigated the effect of this sequence on homologous recombination events. Here we report that the putative fold-back DNA tetraplex structure induces homologous recombination between direct repeat sequences. Interestingly, this recombination event is independent of recA, a major driving force for homologous recombination. We think that the fold-back structure forces the repeat sequences to come into close proximity and therefore leads to strand exchange. Although triplex-induced recombination has been well documented, our results for the first time directly establish the potential of a tetraplex structure to induce recA-independent homologous recombination in vivo. This finding might have a significant implication for site-directed gene deletion in the context of the correction of genetic defects.
Applied Mathematical Sciences, Jan 1, 2009
In this study, a deterministic inventory model in which items are subject to constant deteriorati... more In this study, a deterministic inventory model in which items are subject to constant deterioration and shortages are allowed. The unsatisfied demand is backlogged which is a function of time. The optimal order quantity is derived by minimizing the total cost. The numerical example is given to support the result. The convexity of the cost function is shown numerically. Sensitivity analysis is carried out to analyze the effect of critical parameters on decision variables and the total cost of an inventory system.
Biological chemistry, Jan 1, 2006
We have recently reported that a GC-rich palindromic repeat sequence presumably adopts a stable f... more We have recently reported that a GC-rich palindromic repeat sequence presumably adopts a stable fold-back tetraplex DNA structure under supercoiling. To establish the biological significance of this structure, we inserted this sequence between two direct repeat sequences, separated by 200 bp, in a plasmid. We then investigated the effect of this sequence on homologous recombination events. Here we report that the putative fold-back DNA tetraplex structure induces homologous recombination between direct repeat sequences. Interestingly, this recombination event is independent of recA, a major driving force for homologous recombination. We think that the fold-back structure forces the repeat sequences to come into close proximity and therefore leads to strand exchange. Although triplex-induced recombination has been well documented, our results for the first time directly establish the potential of a tetraplex structure to induce recA-independent homologous recombination in vivo. This finding might have a significant implication for site-directed gene deletion in the context of the correction of genetic defects.
Applied Mathematical Sciences, Jan 1, 2009
In this study, a deterministic inventory model in which items are subject to constant deteriorati... more In this study, a deterministic inventory model in which items are subject to constant deterioration and shortages are allowed. The unsatisfied demand is backlogged which is a function of time. The optimal order quantity is derived by minimizing the total cost. The numerical example is given to support the result. The convexity of the cost function is shown numerically. Sensitivity analysis is carried out to analyze the effect of critical parameters on decision variables and the total cost of an inventory system.