Cyril Ahonsi - Academia.edu (original) (raw)

Papers by Cyril Ahonsi

Journal of Applied Sciences and Environmental Management, 2020

Environmental contamination by waste engine oil in cities all over the world constitute serious p... more Environmental contamination by waste engine oil in cities all over the world constitute serious public health concern because its effects are unwholesome and unquantifiable. This study was carried out to biodegrade waste engine oil using bacterial isolates. Three bacteria were isolated from soil samples that have been contaminated with waste engine oil for over seven years in automechanic workshops at Ebelle. They were analysed using standard methods. The bacterial count was 89.4 ± 0.02cfu/g while the control was 3.6 ± 0.14cfu/g. The bacterial diversity identified in descending order were Bacillus sp. (25.41%), Pseudomonas sp. (22.13%), Corynebacterium sp. (18.44%), Acetobacter sp. (18.03%) and Aeromonas sp. (15.98%). The biodegradation efficiencies of three bacterial isolates; Bacillus, Pseudomonas and Corynebacterium species were observed taking records of change in physicochemical parameters (pH, electrical conductivity and total hydrocarbon content) for 40 days at 10 days interv...

Journal of Applied Sciences and Environmental Management, 2020

Environmental contamination by waste engine oil in cities all over the world constitute serious p... more Environmental contamination by waste engine oil in cities all over the world constitute serious public health concern because its effects are unwholesome and unquantifiable. This study was carried out to biodegrade waste engine oil using bacterial isolates. Three bacteria were isolated from soil samples that have been contaminated with waste engine oil for over seven years in automechanic workshops at Ebelle. They were analysed using standard methods. The bacterial count was 89.4 ± 0.02cfu/g while the control was 3.6 ± 0.14cfu/g. The bacterial diversity identified in descending order were Bacillus sp. (25.41%), Pseudomonas sp. (22.13%), Corynebacterium sp. (18.44%), Acetobacter sp. (18.03%) and Aeromonas sp. (15.98%). The biodegradation efficiencies of three bacterial isolates; Bacillus, Pseudomonas and Corynebacterium species were observed taking records of change in physicochemical parameters (pH, electrical conductivity and total hydrocarbon content) for 40 days at 10 days interv...

J. Appl. Sci. Environ. Manage., 2020

Environmental contamination by waste engine oil in cities all over the world constitute serious p... more Environmental contamination by waste engine oil in cities all over the world constitute serious public health concern because its effects are unwholesome and unquantifiable. This study was carried out to biodegrade waste engine oil using bacterial isolates. Three bacteria were isolated from soil samples that have been contaminated with waste engine oil for over seven years in automechanic workshops at Ebelle. They were analysed using standard methods. The bacterial count was 89.4 ± 0.02cfu/g while the control was 3.6 ± 0.14cfu/g. The bacterial diversity identified in descending order were Bacillus sp. (25.41%), Pseudomonas sp. (22.13%), Corynebacterium sp. (18.44%), Acetobacter sp. (18.03%) and Aeromonas sp. (15.98%). The biodegradation efficiencies of three bacterial isolates; Bacillus, Pseudomonas and Corynebacterium species were observed taking records of change in physicochemical parameters (pH, electrical conductivity and total hydrocarbon content) for 40 days at 10 days interval with waste engine oil sample at concentrations of 10%, 20%, 30% and 40%. Corynebacterium sp. gave the highest percentage reduction in pH values of 52.92% at 10% contamination level while Pseudomonas sp. gave the highest percentage reduction of 53.04%, 52.99% and 53.01% at 20%, 30% and 40% crude oil contamination levels respectively. The pH values of the control was almost the same all through the period of incubation. Bacillus sp. gave the highest percentage increase in electrical conductivity value of 74.17%, 69.93% and 69.91% at 10%, 20% and 40% contamination level respectively while Pseudomonas sp. gave the highest increase of 70.45% at 30% contamination level. Also, the values at day 0 and the control were not so different from each other. Corynebacterium sp. gave the highest percentage reduction in total hydrocarbon content of 95.49%, 95.48%, 96.50% and 96.50% at 10%, 20%, 30% and 40% contamination level respectively. The result revealed a reduction in pH and total hydrocarbon content while there was an increase in electrical conductivity values. This indicated that biodegradation occurred. This study revealed that the bacterial isolates particularly Corynebacterium sp. possess the ability to biodegrade waste engine oil thereby guaranteeing environmental quality and sustained public health for all and sundry.

The anti-proliferation and antioxidant activities of Conyza sumatrensis was carried out using ben... more The anti-proliferation and antioxidant activities of Conyza sumatrensis was carried out using bench-top assay method. The anti-proliferation activities were carried out using methanol extract of the root of Conyza sumatrensis on guinea corn (Sorghum bicolor) radicle at 1-30 mg/ml. While the antioxidant activity was done using superoxide dismuthase (SOD), ferric reducing antioxidant power (FRAP) and α-diphenyl-β-picrylhydrazyl (DPPH) antioxidant test. In addition to the evaluation, the phytochemical constituents of the methanol extract of the root of C. sumatrensis was performed. The extraction of the plant material was done by cold maceration and concentration of the extract was done using rotary evaporator at 40 o C. Results from the study show significant growth inhibitory effect on guinea corn. An average growth length of 2.74 ± 0.20 mm, 2.93 ± 0.44 mm and 2.88 ± 0.40 mm were produced by the radicle of the control seeds of the methanol extract, aqueous fraction and chloroform fraction respectively after 24 hrs. While the seeds treated with 30mg/ml were 1.07 ± 0.44 mm, 1.28 ± 0.24 mm, 0.20 ± 0.12 mm for the methanol extract, aqueous fraction and chloroform fractions were 60.95%, 56.30% and 93.06% reduction in length respectively. After 96hrs, the control recorded an average length of 20.95 ± 4.69 mm, 34.45 ± 4.92 mm and 13.73 ± 2.86 mm in relation to 3.15 ± 1.15 mm, 11.38 ± 1.65 mm and 0.65 ± 20 mm produced by the seeds treated with 30mg/ml of the methanol extract, aqueous fraction and chloroform fraction respectively. This indicates reduction in growth by 88.18%, 66.97% and 95.27% respectively. The plant extract was shown to contain alkaloid, saponins, cardiac glycoside, tannins, flavonoids, steroids, terpenoids and anthraquinone. Also, the antioxidant property of the chloroform fraction, aqueous fraction and methanol extract were 4.86 ± 0.03mmol/min/mg, 2.75 ± 0.02 mmol/min/mg and 3.59 ± 0.64 mmol/ml/mg respectively for SOD test, 351.57 ± 22.03 mg CEQ/100gdw, 148.94 ± 43.47 mg CEQ/100gdw and 339.99 ±15.32 mg CEQ/100gdw respectively for DPPH test and 147.12 ± 71.07 g CEQ/100gdw, 35.16 ± 9.05 mg CEQ/100gdw and 22.28 ± 7.60 mg CEQ/100gdw respectively for FRAP test. Thus, indicating that the chloroform fraction has better antioxidant property than the aqueous and chloroform fractions. In conclusion, the result of this work supports the ethno-medicinal use of the plant in treating tumour-related ailments. However, further investigation using tumour cell line in vitro or in vivo may be necessary to confirm this claim.

African Scientist, 2017

This study was carried out to assess the microbiological quality of ready-to-eat papaya fruit fro... more This study was carried out to assess the microbiological quality of ready-to-eat papaya fruit from selected locations in Benin City. Standard bacteriological techniques using selective/differential media were employed for the isolation, purification and putative identification of selected bacterial isolates present in vended papaya fruits. The susceptibility and resistance of these bacterial isolates to common antibiotics was accessed. The public health implication of the identified isolates were also evaluated using their multiple antibiotic resistance (MAR) index. Pawpaw samples obtained from three locations had colonies which were too numerous to count while samples obtained from eight other locations had counts which ranged from 2.33 x 10 4-1.89 x 10 5 cfu/g. Total staphylococcal count ranged from 8.2 x 10 2-8.21 x 10 4 cfu/g. Coliform count ranged between 4.8 x 10 3-1.68 x 10 4 cfu/g. Salmonella count obtained from the pawpaw samples were in the range of 2.03 x 10 2-1.86 x 10 4 cfu/g while Pseudomonas counts were within the range of 4.2 x 10 3-6.7x10 3 cfu/g. The putative bacterial isolates from vended pawpaw fruit were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Klebsiella oxytoca and Enterococcus faecalis. The isolates were found to be resistant to some of the antibiotics such as Ampicillin, Amoxicillin, Eythromycin and Cefixime. They however, were susceptible to Gentamicin and Ciprofloxacin and were least susceptible to Amoxicillin, Ampicillin and Cefixime. Results obtained are of public health importance as the isolates were found to have an MAR index greater than 0.2.

IOSR, 2022

The pollution of the aquatic environment with heavy metals has become a worldwide problem in rece... more The pollution of the aquatic environment with heavy metals has become a worldwide problem in recent years, due to their recalcitrant nature and most of them have toxic effect on living organisms The study was aimed at determining the concentration of some selected heavy metals namely; Manganese, Iron, Copper and Zinc (Mn, Fe, Cu and Zn) in Tilapia zilli, Synodontis nigrita and Clarias gariepinus from Tagwai dam using standard methods. sample were blended into fine powder and sieved for digestion. The levels of heavy metals were determined using atomic absorption spectrophotometer. The results revealed that Zinc has the highest concentration (33.61 and 64.49mg/kg) and Copper has the lowest concentration (2.78 and 2.70) in both Synodonits nigrita and Clarias gariepinus, and both follow the same trend in order of decreasing: Zn > Fe > Mn > Cu. While in Tilapia fish Iron has the highest concentration (38.98mg/kg) and Manganese has the lowest concentration (4.34mg/kg) and follows the trend in a decreasing order: Fe > Zn > Cu > Mn. All the concentrations obtained for these heavy metals analysis was below the maximum permissible limit recommended by international standard organization, hence they are safe for human consumption.

Journal of Applied Sciences and Environmental Management, 2020

Environmental contamination by waste engine oil in cities all over the world constitute serious p... more Environmental contamination by waste engine oil in cities all over the world constitute serious public health concern because its effects are unwholesome and unquantifiable. This study was carried out to biodegrade waste engine oil using bacterial isolates. Three bacteria were isolated from soil samples that have been contaminated with waste engine oil for over seven years in automechanic workshops at Ebelle. They were analysed using standard methods. The bacterial count was 89.4 ± 0.02cfu/g while the control was 3.6 ± 0.14cfu/g. The bacterial diversity identified in descending order were Bacillus sp. (25.41%), Pseudomonas sp. (22.13%), Corynebacterium sp. (18.44%), Acetobacter sp. (18.03%) and Aeromonas sp. (15.98%). The biodegradation efficiencies of three bacterial isolates; Bacillus, Pseudomonas and Corynebacterium species were observed taking records of change in physicochemical parameters (pH, electrical conductivity and total hydrocarbon content) for 40 days at 10 days interv...

Journal of Applied Sciences and Environmental Management, 2020

Environmental contamination by waste engine oil in cities all over the world constitute serious p... more Environmental contamination by waste engine oil in cities all over the world constitute serious public health concern because its effects are unwholesome and unquantifiable. This study was carried out to biodegrade waste engine oil using bacterial isolates. Three bacteria were isolated from soil samples that have been contaminated with waste engine oil for over seven years in automechanic workshops at Ebelle. They were analysed using standard methods. The bacterial count was 89.4 ± 0.02cfu/g while the control was 3.6 ± 0.14cfu/g. The bacterial diversity identified in descending order were Bacillus sp. (25.41%), Pseudomonas sp. (22.13%), Corynebacterium sp. (18.44%), Acetobacter sp. (18.03%) and Aeromonas sp. (15.98%). The biodegradation efficiencies of three bacterial isolates; Bacillus, Pseudomonas and Corynebacterium species were observed taking records of change in physicochemical parameters (pH, electrical conductivity and total hydrocarbon content) for 40 days at 10 days interv...

J. Appl. Sci. Environ. Manage., 2020

Environmental contamination by waste engine oil in cities all over the world constitute serious p... more Environmental contamination by waste engine oil in cities all over the world constitute serious public health concern because its effects are unwholesome and unquantifiable. This study was carried out to biodegrade waste engine oil using bacterial isolates. Three bacteria were isolated from soil samples that have been contaminated with waste engine oil for over seven years in automechanic workshops at Ebelle. They were analysed using standard methods. The bacterial count was 89.4 ± 0.02cfu/g while the control was 3.6 ± 0.14cfu/g. The bacterial diversity identified in descending order were Bacillus sp. (25.41%), Pseudomonas sp. (22.13%), Corynebacterium sp. (18.44%), Acetobacter sp. (18.03%) and Aeromonas sp. (15.98%). The biodegradation efficiencies of three bacterial isolates; Bacillus, Pseudomonas and Corynebacterium species were observed taking records of change in physicochemical parameters (pH, electrical conductivity and total hydrocarbon content) for 40 days at 10 days interval with waste engine oil sample at concentrations of 10%, 20%, 30% and 40%. Corynebacterium sp. gave the highest percentage reduction in pH values of 52.92% at 10% contamination level while Pseudomonas sp. gave the highest percentage reduction of 53.04%, 52.99% and 53.01% at 20%, 30% and 40% crude oil contamination levels respectively. The pH values of the control was almost the same all through the period of incubation. Bacillus sp. gave the highest percentage increase in electrical conductivity value of 74.17%, 69.93% and 69.91% at 10%, 20% and 40% contamination level respectively while Pseudomonas sp. gave the highest increase of 70.45% at 30% contamination level. Also, the values at day 0 and the control were not so different from each other. Corynebacterium sp. gave the highest percentage reduction in total hydrocarbon content of 95.49%, 95.48%, 96.50% and 96.50% at 10%, 20%, 30% and 40% contamination level respectively. The result revealed a reduction in pH and total hydrocarbon content while there was an increase in electrical conductivity values. This indicated that biodegradation occurred. This study revealed that the bacterial isolates particularly Corynebacterium sp. possess the ability to biodegrade waste engine oil thereby guaranteeing environmental quality and sustained public health for all and sundry.

The anti-proliferation and antioxidant activities of Conyza sumatrensis was carried out using ben... more The anti-proliferation and antioxidant activities of Conyza sumatrensis was carried out using bench-top assay method. The anti-proliferation activities were carried out using methanol extract of the root of Conyza sumatrensis on guinea corn (Sorghum bicolor) radicle at 1-30 mg/ml. While the antioxidant activity was done using superoxide dismuthase (SOD), ferric reducing antioxidant power (FRAP) and α-diphenyl-β-picrylhydrazyl (DPPH) antioxidant test. In addition to the evaluation, the phytochemical constituents of the methanol extract of the root of C. sumatrensis was performed. The extraction of the plant material was done by cold maceration and concentration of the extract was done using rotary evaporator at 40 o C. Results from the study show significant growth inhibitory effect on guinea corn. An average growth length of 2.74 ± 0.20 mm, 2.93 ± 0.44 mm and 2.88 ± 0.40 mm were produced by the radicle of the control seeds of the methanol extract, aqueous fraction and chloroform fraction respectively after 24 hrs. While the seeds treated with 30mg/ml were 1.07 ± 0.44 mm, 1.28 ± 0.24 mm, 0.20 ± 0.12 mm for the methanol extract, aqueous fraction and chloroform fractions were 60.95%, 56.30% and 93.06% reduction in length respectively. After 96hrs, the control recorded an average length of 20.95 ± 4.69 mm, 34.45 ± 4.92 mm and 13.73 ± 2.86 mm in relation to 3.15 ± 1.15 mm, 11.38 ± 1.65 mm and 0.65 ± 20 mm produced by the seeds treated with 30mg/ml of the methanol extract, aqueous fraction and chloroform fraction respectively. This indicates reduction in growth by 88.18%, 66.97% and 95.27% respectively. The plant extract was shown to contain alkaloid, saponins, cardiac glycoside, tannins, flavonoids, steroids, terpenoids and anthraquinone. Also, the antioxidant property of the chloroform fraction, aqueous fraction and methanol extract were 4.86 ± 0.03mmol/min/mg, 2.75 ± 0.02 mmol/min/mg and 3.59 ± 0.64 mmol/ml/mg respectively for SOD test, 351.57 ± 22.03 mg CEQ/100gdw, 148.94 ± 43.47 mg CEQ/100gdw and 339.99 ±15.32 mg CEQ/100gdw respectively for DPPH test and 147.12 ± 71.07 g CEQ/100gdw, 35.16 ± 9.05 mg CEQ/100gdw and 22.28 ± 7.60 mg CEQ/100gdw respectively for FRAP test. Thus, indicating that the chloroform fraction has better antioxidant property than the aqueous and chloroform fractions. In conclusion, the result of this work supports the ethno-medicinal use of the plant in treating tumour-related ailments. However, further investigation using tumour cell line in vitro or in vivo may be necessary to confirm this claim.

African Scientist, 2017

This study was carried out to assess the microbiological quality of ready-to-eat papaya fruit fro... more This study was carried out to assess the microbiological quality of ready-to-eat papaya fruit from selected locations in Benin City. Standard bacteriological techniques using selective/differential media were employed for the isolation, purification and putative identification of selected bacterial isolates present in vended papaya fruits. The susceptibility and resistance of these bacterial isolates to common antibiotics was accessed. The public health implication of the identified isolates were also evaluated using their multiple antibiotic resistance (MAR) index. Pawpaw samples obtained from three locations had colonies which were too numerous to count while samples obtained from eight other locations had counts which ranged from 2.33 x 10 4-1.89 x 10 5 cfu/g. Total staphylococcal count ranged from 8.2 x 10 2-8.21 x 10 4 cfu/g. Coliform count ranged between 4.8 x 10 3-1.68 x 10 4 cfu/g. Salmonella count obtained from the pawpaw samples were in the range of 2.03 x 10 2-1.86 x 10 4 cfu/g while Pseudomonas counts were within the range of 4.2 x 10 3-6.7x10 3 cfu/g. The putative bacterial isolates from vended pawpaw fruit were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Klebsiella oxytoca and Enterococcus faecalis. The isolates were found to be resistant to some of the antibiotics such as Ampicillin, Amoxicillin, Eythromycin and Cefixime. They however, were susceptible to Gentamicin and Ciprofloxacin and were least susceptible to Amoxicillin, Ampicillin and Cefixime. Results obtained are of public health importance as the isolates were found to have an MAR index greater than 0.2.

IOSR, 2022

The pollution of the aquatic environment with heavy metals has become a worldwide problem in rece... more The pollution of the aquatic environment with heavy metals has become a worldwide problem in recent years, due to their recalcitrant nature and most of them have toxic effect on living organisms The study was aimed at determining the concentration of some selected heavy metals namely; Manganese, Iron, Copper and Zinc (Mn, Fe, Cu and Zn) in Tilapia zilli, Synodontis nigrita and Clarias gariepinus from Tagwai dam using standard methods. sample were blended into fine powder and sieved for digestion. The levels of heavy metals were determined using atomic absorption spectrophotometer. The results revealed that Zinc has the highest concentration (33.61 and 64.49mg/kg) and Copper has the lowest concentration (2.78 and 2.70) in both Synodonits nigrita and Clarias gariepinus, and both follow the same trend in order of decreasing: Zn > Fe > Mn > Cu. While in Tilapia fish Iron has the highest concentration (38.98mg/kg) and Manganese has the lowest concentration (4.34mg/kg) and follows the trend in a decreasing order: Fe > Zn > Cu > Mn. All the concentrations obtained for these heavy metals analysis was below the maximum permissible limit recommended by international standard organization, hence they are safe for human consumption.