John Bumpus | University of Northern Iowa (original) (raw)
Papers by John Bumpus
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Journal of Biological Chemistry, May 1, 1981
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Biochemistry, May 1, 1992
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Applied Microbiology and Biotechnology, Sep 1, 1989
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The wood-rotting fungus Phanerochaete chrysosporium is able to degrade a wide variety of environm... more The wood-rotting fungus Phanerochaete chrysosporium is able to degrade a wide variety of environmentally-persistent organic pollutants to carbon dioxide. The unique biodegradative abilities of this fungus are due, in part, to lignin peroxidases, oxidative enzymes that are secreted in response to nutrient deprivation. Lignin peroxidases catalyze the initial oxidation of many of the organic pollutants that are degraded by this fungus. They also mediate the initial oxidation of N,N,N{prime},N{prime},N{double_prime},N{double_prime}-hexamethylpararosaniline, several azo dyes and certain polycyclic aromatic hydrocarbons. Lignin peroxidases also mediate oxidative dechlorination. For example, lignin peroxidases oxidize pentachlorophenol to 2,3,5,6-tetrachloro-2,5-cyclohexadiene-1,4-dione. Similarly, these enzymes mediate oxidative oligomerization of 4-chloroaniline, resulting in production of several dimers, trimers and tetramers and net dechlorination of the aromatic ring. Interestingly, m...
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Applied and Environmental Microbiology, 1989
The ability of the white rot fungus Phanerochaete chrysosporium to degrade polycyclic aromatic hy... more The ability of the white rot fungus Phanerochaete chrysosporium to degrade polycyclic aromatic hydrocarbons (PAHs) that are present in anthracene oil (a distillation product obtained from coal tar) was demonstrated. Analysis by capillary gas chromatography and high-performance liquid chromatography showed that at least 22 PAHs, including all of the most abundant PAH components present in anthracene oil, underwent 70 to 100% disappearance during 27 days of incubation with nutrient nitrogen-limited cultures of this fungus. Because phenanthrene is the most abundant PAH present in anthracene oil, this PAH was selected for further study. In experiments in which [14C]phenanthrene was incubated with cultures of P. chrysosporium containing anthracene oil for 27 days, it was shown that 7.7% of the recovered radiolabeled carbon originally present in [14C]phenanthrene was metabolized to 14CO2 and 25.2% was recovered from the aqueous fraction, while 56.1 and 11.0% were recovered from the methyl...
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Journal of Cell Biology 107(6 Part, Dec 8, 1988
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Journal of Chemical Education, 2007
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The Journal of Physical Chemistry A, 2018
A straightforward procedure using density functional theory (M06 2X) coupled with a group-equival... more A straightforward procedure using density functional theory (M06 2X) coupled with a group-equivalent approach is described that was used to calculate gas-phase heat of formation (Δf H°g,298) values for buckminsterfullerene (C60), C70 fullerene (C70), corannulene, coronene, and sumanene. This procedure was also used to calculate exceptionally accurate Δf H°g,298 values for a variety of single-ring aromatic and 2-7 ring polycyclic aromatic hydrocarbons (PAHs) as well as a large selection of other hydrocarbons and phenols. The approach described herein is internally consistent, and results for C60, C70, corannulene, coronene, and sumanene are in very close agreement with results reported by others who used higher-level computational theory. Statistical analysis of a test set containing benzene and 18 two to seven ring PAHs demonstrated that by using this approach a mean absolute deviation (MAD) and a root-mean-square deviation (RMSD) of 0.8 and 1.3 kJ/mol, respectively, were achieved for reference/experimental Δf H°g,298 values versus calculated/predicted Δf H°g,298 values. For statistical analysis of a larger test set containing 235 aromatic and aliphatic hydrocarbons and phenols, a MAD and a RMSD of 1.2 and 1.9 kJ/mol, respectively, were achieved for reference/experimental Δf H°g,298 values versus calculated/predicted Δf H°g,298 values.
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Microsomes, Drug Oxidations and Chemical Carcinogenesis, 1980
Cytochrome P-450 and NADPH cytochrome c (P-450) reductase were purified from bovine adrenocortica... more Cytochrome P-450 and NADPH cytochrome c (P-450) reductase were purified from bovine adrenocortical (BAC) microsomes. Cytochrome P-450 AM-1 purified to 10–12 nanomoles P-450/mg protein by AM 2 SO 4 fractionation and hydrophobic interaction chromatography showed essentially one band by SDS-page (>95%) with a MW = 52,000. Fraction P-450 AM-2 with a specific content of 3-4 nanomoles P-450/mg protein contained two hemeproteins with M.W. of 49,000 and 52,000. Partially purified reductase reduced cytochrome P-450. Adrenodoxin was not required nor did it stimulate reduction. Photoaffinity labeling studies show the inhibitor derived label 1-(4-azidophenyl)imidazole to have high affinity for BAC microsomal P-450. Labeling experiments using partially purified BAC microsomal P-450 afforded stoichiometric label incorporation.
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Soil Biochemistry, 2021
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Microsomes, Drug Oxidations and Chemical Carcinogenesis, 1980
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Journal of Biological Chemistry, 1982
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Journal of Biological Chemistry, May 1, 1981
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Biochemistry, May 1, 1992
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Applied Microbiology and Biotechnology, Sep 1, 1989
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The wood-rotting fungus Phanerochaete chrysosporium is able to degrade a wide variety of environm... more The wood-rotting fungus Phanerochaete chrysosporium is able to degrade a wide variety of environmentally-persistent organic pollutants to carbon dioxide. The unique biodegradative abilities of this fungus are due, in part, to lignin peroxidases, oxidative enzymes that are secreted in response to nutrient deprivation. Lignin peroxidases catalyze the initial oxidation of many of the organic pollutants that are degraded by this fungus. They also mediate the initial oxidation of N,N,N{prime},N{prime},N{double_prime},N{double_prime}-hexamethylpararosaniline, several azo dyes and certain polycyclic aromatic hydrocarbons. Lignin peroxidases also mediate oxidative dechlorination. For example, lignin peroxidases oxidize pentachlorophenol to 2,3,5,6-tetrachloro-2,5-cyclohexadiene-1,4-dione. Similarly, these enzymes mediate oxidative oligomerization of 4-chloroaniline, resulting in production of several dimers, trimers and tetramers and net dechlorination of the aromatic ring. Interestingly, m...
Bookmarks Related papers MentionsView impact
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Applied and Environmental Microbiology, 1989
The ability of the white rot fungus Phanerochaete chrysosporium to degrade polycyclic aromatic hy... more The ability of the white rot fungus Phanerochaete chrysosporium to degrade polycyclic aromatic hydrocarbons (PAHs) that are present in anthracene oil (a distillation product obtained from coal tar) was demonstrated. Analysis by capillary gas chromatography and high-performance liquid chromatography showed that at least 22 PAHs, including all of the most abundant PAH components present in anthracene oil, underwent 70 to 100% disappearance during 27 days of incubation with nutrient nitrogen-limited cultures of this fungus. Because phenanthrene is the most abundant PAH present in anthracene oil, this PAH was selected for further study. In experiments in which [14C]phenanthrene was incubated with cultures of P. chrysosporium containing anthracene oil for 27 days, it was shown that 7.7% of the recovered radiolabeled carbon originally present in [14C]phenanthrene was metabolized to 14CO2 and 25.2% was recovered from the aqueous fraction, while 56.1 and 11.0% were recovered from the methyl...
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Journal of Cell Biology 107(6 Part, Dec 8, 1988
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Journal of Chemical Education, 2007
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The Journal of Physical Chemistry A, 2018
A straightforward procedure using density functional theory (M06 2X) coupled with a group-equival... more A straightforward procedure using density functional theory (M06 2X) coupled with a group-equivalent approach is described that was used to calculate gas-phase heat of formation (Δf H°g,298) values for buckminsterfullerene (C60), C70 fullerene (C70), corannulene, coronene, and sumanene. This procedure was also used to calculate exceptionally accurate Δf H°g,298 values for a variety of single-ring aromatic and 2-7 ring polycyclic aromatic hydrocarbons (PAHs) as well as a large selection of other hydrocarbons and phenols. The approach described herein is internally consistent, and results for C60, C70, corannulene, coronene, and sumanene are in very close agreement with results reported by others who used higher-level computational theory. Statistical analysis of a test set containing benzene and 18 two to seven ring PAHs demonstrated that by using this approach a mean absolute deviation (MAD) and a root-mean-square deviation (RMSD) of 0.8 and 1.3 kJ/mol, respectively, were achieved for reference/experimental Δf H°g,298 values versus calculated/predicted Δf H°g,298 values. For statistical analysis of a larger test set containing 235 aromatic and aliphatic hydrocarbons and phenols, a MAD and a RMSD of 1.2 and 1.9 kJ/mol, respectively, were achieved for reference/experimental Δf H°g,298 values versus calculated/predicted Δf H°g,298 values.
Bookmarks Related papers MentionsView impact
Microsomes, Drug Oxidations and Chemical Carcinogenesis, 1980
Cytochrome P-450 and NADPH cytochrome c (P-450) reductase were purified from bovine adrenocortica... more Cytochrome P-450 and NADPH cytochrome c (P-450) reductase were purified from bovine adrenocortical (BAC) microsomes. Cytochrome P-450 AM-1 purified to 10–12 nanomoles P-450/mg protein by AM 2 SO 4 fractionation and hydrophobic interaction chromatography showed essentially one band by SDS-page (>95%) with a MW = 52,000. Fraction P-450 AM-2 with a specific content of 3-4 nanomoles P-450/mg protein contained two hemeproteins with M.W. of 49,000 and 52,000. Partially purified reductase reduced cytochrome P-450. Adrenodoxin was not required nor did it stimulate reduction. Photoaffinity labeling studies show the inhibitor derived label 1-(4-azidophenyl)imidazole to have high affinity for BAC microsomal P-450. Labeling experiments using partially purified BAC microsomal P-450 afforded stoichiometric label incorporation.
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Soil Biochemistry, 2021
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Microsomes, Drug Oxidations and Chemical Carcinogenesis, 1980
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Journal of Biological Chemistry, 1982
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