Michael Evangelopoulos | Northwestern University (original) (raw)
Uploads
Papers by Michael Evangelopoulos
Clinical and Translational Medicine, 2019
Nanoparticles have seen considerable popularity as effective tools for drug delivery. However, no... more Nanoparticles have seen considerable popularity as effective tools for drug delivery. However, non-specific targeting continues to remain a challenge. Recently, biomimetic nanoparticles have emerged as an innovative solution that exploits biologically-derived components to improve therapeutic potential. Specifically, cell membrane proteins extracted from various cells (i.e., leukocytes, erythrocytes, platelets, mesenchymal stem cells, cancer) have shown considerable promise in bestowing nanoparticles with increased circulation and targeting efficacy. Traditional nano-particles can be detected and removed by the immune system which significantly hinders their clinical success. Bio-mimicry has been proposed as a promising approach to overcome these limitations. In this review, we highlight the current trends in biomimetic nanoparticles and describe how they are being used to increase their chemotherapeutic effect in cancer treatment.
Nature Nanotechnology, 2012
LLVs were produced by cloaking NPS with cellular membranes isolated from freshly harvested leukoc... more LLVs were produced by cloaking NPS with cellular membranes isolated from freshly harvested leukocytes . The membranes
Journal of Biomedical Materials Research Part A, 2013
The careful scrutiny of drug delivery systems is essential to evaluate and justify their potentia... more The careful scrutiny of drug delivery systems is essential to evaluate and justify their potential for the clinic. Among the various studies necessary for preclinical testing, the impact of degradation is commonly overlooked. In this article, we investigate the effect of fabrication (porosity and nucleation layer) and environment (buffer and pH) factors on the degradation kinetics of multistage nanovectors (MSV) composed of porous silicon. The degradation by-products of MSV were exposed to endothelial cells and analyzed for detrimental effects on cellular internalization, architecture, proliferation, and cell cycle. Increases in porosity resulted in accelerated degradation exhibiting smaller-sized particles at comparable times. Removal of the nucleation layer (thin layer of small pores formed during the initial steps of etching) trig-gered a premature collapse of the entire central porous region of MSV. Variations in buffers prompted a faster degradation rate yielding smaller MSV within faster time frames, whereas increases in pH stimulated erosion of MSV and thus faster degradation. In addition, exposure to these degradation by-products provoked negligible impact on the proliferation and cell cycle phases on primary endothelial cells. In this study, we propose methods that lay the foundation for future investigations toward understanding the impact of the degradation of drug delivery platforms. V C 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3540-3549, 2014.
Clinical and Translational Medicine, 2019
Nanoparticles have seen considerable popularity as effective tools for drug delivery. However, no... more Nanoparticles have seen considerable popularity as effective tools for drug delivery. However, non-specific targeting continues to remain a challenge. Recently, biomimetic nanoparticles have emerged as an innovative solution that exploits biologically-derived components to improve therapeutic potential. Specifically, cell membrane proteins extracted from various cells (i.e., leukocytes, erythrocytes, platelets, mesenchymal stem cells, cancer) have shown considerable promise in bestowing nanoparticles with increased circulation and targeting efficacy. Traditional nano-particles can be detected and removed by the immune system which significantly hinders their clinical success. Bio-mimicry has been proposed as a promising approach to overcome these limitations. In this review, we highlight the current trends in biomimetic nanoparticles and describe how they are being used to increase their chemotherapeutic effect in cancer treatment.
Nature Nanotechnology, 2012
LLVs were produced by cloaking NPS with cellular membranes isolated from freshly harvested leukoc... more LLVs were produced by cloaking NPS with cellular membranes isolated from freshly harvested leukocytes . The membranes
Journal of Biomedical Materials Research Part A, 2013
The careful scrutiny of drug delivery systems is essential to evaluate and justify their potentia... more The careful scrutiny of drug delivery systems is essential to evaluate and justify their potential for the clinic. Among the various studies necessary for preclinical testing, the impact of degradation is commonly overlooked. In this article, we investigate the effect of fabrication (porosity and nucleation layer) and environment (buffer and pH) factors on the degradation kinetics of multistage nanovectors (MSV) composed of porous silicon. The degradation by-products of MSV were exposed to endothelial cells and analyzed for detrimental effects on cellular internalization, architecture, proliferation, and cell cycle. Increases in porosity resulted in accelerated degradation exhibiting smaller-sized particles at comparable times. Removal of the nucleation layer (thin layer of small pores formed during the initial steps of etching) trig-gered a premature collapse of the entire central porous region of MSV. Variations in buffers prompted a faster degradation rate yielding smaller MSV within faster time frames, whereas increases in pH stimulated erosion of MSV and thus faster degradation. In addition, exposure to these degradation by-products provoked negligible impact on the proliferation and cell cycle phases on primary endothelial cells. In this study, we propose methods that lay the foundation for future investigations toward understanding the impact of the degradation of drug delivery platforms. V C 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3540-3549, 2014.