Abdou K Allayeh | National Research Centre (original) (raw)

Papers by Abdou K Allayeh

Journal of Genetic Engineering and Biotechnology

Background Obesity is one of the most serious problems over the world. MicroRNAs have developed a... more Background Obesity is one of the most serious problems over the world. MicroRNAs have developed as main mediators of metabolic processes, playing significant roles in physiological processes. Thus, the present study aimed to evaluate the expressions of (miR-15a, miR-Let7, miR-344, and miR-365) and its relationship with the different classes in obese patients. Methods A total of 125 individuals were enrolled in the study and classified into four groups: healthy non-obese controls (n = 50), obese class I (n = 24), obese class II (n = 17), and obese class III (n = 34) concerning body mass index (BMI < 30 kg/m2, BMI 30–34.9 kg/m2, BMI 35–39.9 kg/m2 and BMI ≥ 40 kg/m2, respectively). BMI and the biochemical measurements (fasting glucose, total cholesterol, triglycerides, HDL and LDL, urea, creatinine, AST, and ALT) were determined. The expressions of (miR-15a, miR-Let7, miR-344, and miR-365) were detected through quantitative real-time PCR (RT-qPCR). Results There was a significant di...

The main objective of this study is to evaluate the quality of wastewater by molecular identifica... more The main objective of this study is to evaluate the quality of wastewater by molecular identification of enteroviruses, rotaviruses, and adenoviruses in wastewater samples collected from the ElSerw wastewater treatment facility in Damietta Governorate, Egypt. An additional objective is to assess the usefulness of these viruses as markers of viral reduction during wastewater treatment. A treatment facility's inflow and discharge were sampled 48 times. The incidence of enteric viruses was found in 29 wastewater samples (60.4%). 6.25% (3/48), 0% (0/48), 37.5% (18/48), and 20.8% (10/48) of the samples tested positive for enteroviruses (EVs), noroviruses, rotaviruses, and adenoviruses, respectively. Co-infections with two or more viruses were found in 10.4% (5/48) and 2% (1/48) of all cases, respectively. The viral burden in the wastewater treatment plant's discharge effluents dropped non-significantly when compared to intake samples. According to our findings, rotaviruses and adenoviruses have been found in 10 outlet effluent samples. The removal rates for enteroviruses, rotaviruses and adenoviruses were 39%, 61.5% and 33.3%, respectively. As a result of their high frequency and lower removal rates, both rotaviruses and adenoviruses were deemed an appropriate indicator of human enteric viral reduction during the wastewater treatment process.

European journal of medicinal chemistry, Jun 1, 2023

Viruses

Human adenoviruses (HAdV) are a prevalent cause of diarrhea in children all over the world. Adeno... more Human adenoviruses (HAdV) are a prevalent cause of diarrhea in children all over the world. Adenoviral infections are responsible for 2% to 10% of diarrheic cases. A long-term investigation was required to gain better knowledge about the incidence of HAdV in Egypt. Herein, we conducted 5 years of detection, isolation, and genotyping of HAdV in fecal and sewage samples from 2016 to 2020, in Cairo, Egypt using molecular and cell culture assays. Human adenoviruses were identified in 35 of 447 fecal samples (7.8%), but only 53.3% (64/120) of the sewage samples. Children under the age of two had the highest positive rate for HAdV infection (77.1%). Species F of HAdV was the most common prevalent genotype in fecal and sewage samples, at 88.5% and 85.9%, respectively. The most prevalent genotypes detected in fecal samples were HAdV-41 (71.2%), HAdV-40 (17.2%), HAdV-6 (5.7%), and HAdV-1 (5.7%). In contrast, the most common genotypes in sewage samples were HAdV-41 (64%), HAdVs-40 (21.8%), HA...

Biology, Sep 28, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Biocatalysis and Agricultural Biotechnology

Chemistry & Biodiversity, Sep 12, 2022

International Journal of Biological Macromolecules

Asian Journal of Water, Environment and Pollution, 2018

Rotavirus is one of the most common enteric viruses that can be transmitted via contaminated wate... more Rotavirus is one of the most common enteric viruses that can be transmitted via contaminated water globally. From the hygienic point of view, the occurrence of rotaviruses in drinking water is not acceptable. Investigations about the presence of rotaviruses in tap water are very limited in Egypt. Overall 72 tap water samples were collected and concentrated for the detection of rotavirus group A (RV-A) VP6 gene using RT-PCR. The positive samples for RV-A were further analyzed using multiplex semi-nested RT-PCR for identification P and G rotavirus genotypes. A total of 8.3% tap water samples were positive for rotavirus group A using nested RT-PCR. The temporal distribution showed that rotaviruses isolates were noticed in three seasons, 16.7% (3/18) in autumn, 11.1% (2/18) in winter and 5.6% (1/18) in spring, while in summer there were no positive samples. Statistically, seasonal variation had no considerable effect on the prevalence of RV-A in tap water samples. About 83.3% of G-type rotaviruses were belonging to G1-type, while 50% of the rotaviruses strains were P[8] and 16.7% were P[6]. In conclusion, the presence of RV-A into tap water represented a public health risk and our findings demonstrated the urgent need to add viral parameters to water quality surveillance.

Frontiers in Oncology, 2020

International Journal of Environmental Research and Public Health

Enteric viruses (EVs) occurrence within aquatic environments varies and leads to significant risk... more Enteric viruses (EVs) occurrence within aquatic environments varies and leads to significant risk on public health of humans, animals, and diversity of aquatic taxa. Early and efficacious recognition of cultivable and fastidious EVs in aquatic systems are important to ensure the sanitary level of aquatic water and implement required treatment strategies. Herein, we provided a comprehensive overview of the conventional and up-to-date eco-genomic tools for aquatic biomonitoring of EVs, aiming to develop better water pollution monitoring tools. In combination with bioinformatics techniques, genetic tools including cloning sequencing analysis, DNA microarray, next-generation sequencing (NGS), and metagenomic sequencing technologies are implemented to make informed decisions about the global burden of waterborne EVs-associated diseases. The data presented in this review are helpful to recommend that: (1) Each viral pollution detection method has its own merits and demerits; therefore, it...

Malaysian Journal of Microbiology, 2021

Aims: Infectious bronchitis virus (IBV) is a highly contagious, acute viral respiratory disease t... more Aims: Infectious bronchitis virus (IBV) is a highly contagious, acute viral respiratory disease that mostly affects chickens. The poultry sector has suffered enormous losses as a result of IBV. Currently, live attenuated vaccines are routinely used to prevent and control IBV. However, due to the enormous genetic variety, vaccinations are becoming ineffective, with low cross-protection effects among vaccine serotypes. The present study aimed at investigating the possible antiviral effects of curcumin, epigallocatechin gallate (EGCG) and their mixtures against IBV in vivo. Methodology and results: Curcumin, EGCG and their combinations were administered to infected and uninfected chicken groups and viral load titers were determined by real-time PCR. The clinical symptoms of both the negative and positive control groups were also compared. Finally, the trachea tissues of each group were examined histopathologically. According to our findings, the viral titer and the clinical signs dropped significantly during the pretreatment infection procedure. Curcumin, EGCG and their combinations also show significant antiviral activities. Conclusion, significance and impact of study: This study clearly shown that natural compounds and their combinations, such as curcumin or/and ECGC can reduce viral pathogenicity in vivo, suggesting that they might have therapeutic implications in the poultry sector.

International Journal of Molecular Biology: Open Access, Aug 2, 2019

Renal Replacement Therapy, 2021

Background Despite the fact that the fundamental characteristics of coronavirus disease-2019 (COV... more Background Despite the fact that the fundamental characteristics of coronavirus disease-2019 (COVID-19) are respiratory manifestations, multi-organ failure including the kidney has been documented. There are no clear comparisons of COVID-19 cases with and without acute kidney injury (AKI) to show whether there are aspects of acute kidney injury progression path or outcome that are unique to this disease. Methods In this work, we analyzed the data of 734 COVID-19 cases admitted to the Ahmad Maher Teaching Hospital in Cairo, Egypt, between June 6 and July 25, 2020. Data on demographics, comorbidities, laboratory results, and outcomes were assessed. To assess the incidence rate of AKI in Egyptian COVID-19 patients, comparisons were carried out between home-isolated COVID-19 patients, hospitalized COVID-19 patients, and ICU COVID-19-patients with or without AKI. Results AKI was more common in hospitalized mild COVID-19 patients than in home-isolated and ICU COVID-19 patients (15.0% vers...

The inhibitory effect and mode of action of cell free supernatants (CFS) of six yogurts metabolit... more The inhibitory effect and mode of action of cell free supernatants (CFS) of six yogurts metabolites were evaluated against Coxsackie B3 virus using MTT colorimetric and virus-induced cytopathic reduction methods. Results showed that the CFS containing the metabolites of yogurt fermented with probiotic bacteria have high antiviral activities against CVB3 in vitro and in vivo for all the CFS of yogurts metabolites. It may represent a potential therapeutic agent for viral myocarditis and can be used as functional dairy fermented food with a broad therapeutic potential.

Iranian Journal of Public Health, 2020

Background: In 2013, WHO estimated the annual diarrheal mortality rate among children less than f... more Background: In 2013, WHO estimated the annual diarrheal mortality rate among children less than five years in Egypt was 24 deaths per 100.000, 2% was due to rotavirus infection. Methods: Eight water samples were collected monthly from the Nile water stream passing through Giza over 12 months during June 2016 to May 2017. Totally, ninety-six water samples were collected and concentrated for the detection of rotavirus group A (RV-A) using RT-PCR. Multiplex semi-nested RT-PCR was performed to identify the genotypes P and G of RV-A. Results: The detection rate of RV-A was 18.75% (18/96), whereas the rate of rotavirus genotypes G and P were 61% (11/18) and 50% (9/18), respectively. Rotaviruses G1P[8] and G1P[4] were the most common genotypes identified in our survey. In addition, the seasonal distribution findings demonstrated that the highest detection rate was 37.5% in the winter season, followed by 20.8%, 12.5% and 4% in spring, autumn and summer, respectively. Conclusion: Multiplex s...

Archives of Pediatric Infectious Diseases, 2018

This research aimed at studying the detection and genotyping of rotavirus, enteric adenovirus, an... more This research aimed at studying the detection and genotyping of rotavirus, enteric adenovirus, and astrovirus in Egyptian hospitalized children below five years old, associated with acute diarrhea. In total, 119 fecal samples were obtained from the target population, admitted to Abu El Reesh hospital in Cairo, Egypt. Based on the findings, the detection rate of at least one viral infection was 36.7% in children below five years old, whereas, the overall detection rate of rotavirus, adenovirus, and coinfection was 31%, 6.7%, and 0.8%, respectively. No astrovirus infection was observed in this study. Spring was the peak season for rotavirus and enteric adenovirus. The results showed that higher rates of rotavirus (78%) and enteric adenovirus (100%) were identified in children less than two years of age. The dominant genotypes were G1P [8] (29.7%), G3P [8] (27%), and G1P [4] (18.9%) for rotaviruses and genotype 41 for enteric adenoviruses. Uncommon genotypes G1P [6] and G9P [8] were also detected in five (13.5%) and four (10.8%) samples, respectively. The present findings indicated the high incidence of rotavirus and adenovirus in children below two years old, thus, highlighting the necessity of vaccine development to reduce the incidence of acute viral gastroenteritis in Egypt.

Egyptian Pharmaceutical Journal, 2018

Background According to recent statistics by the WHO, the annual mortality rate associated with d... more Background According to recent statistics by the WHO, the annual mortality rate associated with diarrhea is 30 deaths per 100 000 among Egyptian children younger than 5 years. Nearly 3.9% of the reported deaths are because of rotavirus infection. It is necessary to look for alternative treatment against rotavirus. Aim and methods The aim of this study is to investigate the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts of the fruiting bodies of Agaricus spp and Pleurotus ostreatus against rotavirus infection using cell culture-based MTT assay. Results The tested extracts demonstrated significant inhibition effects against rotavirus infection up to 96.7, 90.6, 86.3, and 83.1% at concentration of 1000 μg/ml of P. ostreatus EtOHE, P. ostreatus AqE, Agaricus spp EtOHE, and Agaricus spp AqE, respectively, when added at zero time of the infection. Nothing was observed when extracts were added after viral infection. The synergistic activity was observed when different extracts were combined. Our results exhibited an inhibitory effect against different phases of rotavirus infection. Conclusion The use of edible mushrooms as a potential antiviral substance might be an alternative treatment against rotavirus infection. Nonetheless, more investigations are requiring for studying the efficacy in vivo and for segregating their fractions, which might clarify the mechanism of the inhibitory effect.