Prof. Mosaad Abdel-Wahhab | National Research Centre (original) (raw)
Papers by Prof. Mosaad Abdel-Wahhab
Recent Patents on Food, Nutrition & Agriculture, Dec 31, 2021
Molecular Neurobiology, Feb 20, 2016
Physiology and Molecular Biology of Plants, Aug 1, 2021
Mycorrhizal symbiosis is generally considered effective in ameliorating plant tolerance to abioti... more Mycorrhizal symbiosis is generally considered effective in ameliorating plant tolerance to abiotic stress by altering gene expression, and evaluation of genes involved in ion homeostasis and nutrient uptake. This study aimed to use arbuscular mycorrhizal fungus (AMF) to alleviate salinity stress and analyse relevant gene expression in pistachio plants under No/NaCl stress in greenhouse conditions. Arbuscular mycorrhizal symbiosis was used to study the physiological responses, ion distribution and relevant gene expression in pistachio plants under salinity stress. After four months of symbiosis, mycorrhizal root colonization showed a significant reduction in all tested parameters under salt stress treatment compared to non-saline treatment. Salinity affected the morphological traits, and decreased the nutrient content including N, P, Mg and Fe as well as K/Na and Ca/Na ratios, relative water content (RWC), membrane stability index (MSI), and increased the concentration of K, Ca and Na nutrient, glycine betaine, ROS and MDA. Inoculation of seedlings with AMF mitigated the negative effects of salinity on plant growth as indicated by increasing the root colonization, morphological traits, glycine betaine, RWC and MSI. Specifically, under salinity stress, shoot and root dry weight, P and Fe nutrient content, K/Na and Ca/Na ratio of AMF plants were increased by 53.2, 48.6, 71.6, 60.2, 87.5, and 80.1% respectively, in contrast to those of the NMF plants. The contents of Na, O2•− and MDA in AMF plants were significantly decreased by 66.8, 36.8, and 23.1%, respectively at 250 mM NaCl. Moreover, salinity markedly increased SOS1, CCX2 and SKOR genes expression and the inoculation with AMF modulated these genes expression; however, NRT2.4, PHO1 and PIP2.4 gene expressions were increased by salinity and AMF. It could be concluded that inoculation of AMF with Rhizophagus irregularis conferred a larger endurance towards soil salinity in pistachio plants and stimulate the nutrient uptake and ionic homeostasis maintenance, superior RWC and osmoprotection, toxic ion partitioning, maintaining membrane integrity and the ion-relevant genes expression.
Abstract Deoxynivalenol (DON) is a mycotoxin mainly produced by Fusarium spp. It is highly toxic ... more Abstract Deoxynivalenol (DON) is a mycotoxin mainly produced by Fusarium spp. It is highly toxic and widely spread dissemination. DON is considered one of the most important trichothecenes which can generate free radicals, mainly reactive oxygen species (ROS), which enhances lipid peroxidation, reduces the activity of antioxidant enzymes, and eventually leads to apoptosis. Thus, oxidative stress is considered one of the most important mechanisms in DON toxicity. Natural antioxidant agents are considered the most effective to protect against DON for human and animals. In this chapter, we review the molecular mechanisms underlying DON-induced oxidative stress. We also summarized the current literature regarding the silymarin (SIL) and inulin (IN) as antioxidant agents for the protection against DON-induced oxidative stress. This chapter provides an inclusive overview of the mechanism of DON-induced oxidative stress and the mechanisms of antioxidant activity induced by SIL and IN against DON.
Journal of Aging Research, Nov 29, 2019
Soft nanoscience letters, 2015
Archives of Toxicology, Sep 14, 2007
Food and Chemical Toxicology, Jul 1, 2007
Aflatoxins are potent toxins and carcinogens which can be excreted in the milk of exposed lactati... more Aflatoxins are potent toxins and carcinogens which can be excreted in the milk of exposed lactating mothers mainly in the form of aflatoxin M(1) (AFM(1)). We previously evaluated the level and frequency of AFM(1) in breast milk in a group of Egyptian mothers attending the New El-Qalyub Hospital, Qalyubiyah governorate, Egypt. In this study, fifty of those women who were AFM(1) positive were revisited monthly for 12 months to assess the temporal variation in breast milk AFM(1). AFM(1) was detected in 248 of 443 (56%) samples. In a multilevel model of the data there was a highly significant (p<0.001) effect of month of sampling on the frequency of AFM(1) detection with summer months having the highest frequency (>80%) and winter months the lowest frequency (<20%) of detection. AFM(1) was observed most frequently in June [OR 63, 95% CI (7.6, 522)]. The level of AFM(1) detection also followed this seasonal pattern with highest mean level in July (64 pg/ml milk, range 6.3-497 pg/ml milk) and the lowest mean level in January (8 pg/ml milk, range 4.2-108 pg/ml milk). The duration of lactation [p=0.0035, OR=1.08, 95% CI (1.02, 1.13)], and peanut consumption [p=0.06, OR=1.69, 95% CI (0.9, 2.9)] also contributed to the model. The identification and understanding of factors determining the presence of toxicants in human milk is important and may provide a knowledge driven basis for controlling the transfer of chemicals to infants.
International Journal of Halal Research, Dec 1, 2019
International Journal of Pharmaceutics, Apr 1, 2022
Lung cancer is one of the most common types of malignant tumors of the respiratory system and has... more Lung cancer is one of the most common types of malignant tumors of the respiratory system and has the highest rates of incidence and mortality of malignant tumors. This study aimed to synthesize and characterize berberine-loaded chitosan nanoparticles (BBR-COSNPs) and to evaluate their protective effects against urethane-induced lung cancer. Forty male albino mice were divided into four groups, with the first serving as a negative control and the other three groups were injected intraperitoneally with urethane (1 mg/kg b.w) each other day for one week then group 2 was served as a positive control, however, groups 3 and 4 were treated orally with a daily dose of BBR or BBR-COSNPs (75 mg/kg b.w) for 10 consecutive weeks. Blood and lung tissue samples are collected for laboratory assay. The BBR-COSNPs were spherical, with an average particle size of 45.56 nm and zeta potential of 39.82 1.82 mV. The in vivo data demonstrated that mice given urethane alone had a significant increase in MDA, NO, NF-κB level, HIF1-α, and COX-2-positive expression in the lung tissue and serum VEGFR2, ALT, AST, urea, and creatinine accompanied with a significant decrease in GSH, SOD, caspase 9 in the lung tissue and serum BAX. Co-treatment with BBR-COSNPs suppressed lung cancer growth and promoted apoptosis by modulating serum BAX and lung caspase 9 gene expressions. In addition, BBR-COSNPs inhibited tumor angiogenesis by reduction in levels of serum VEGFR2 and lung HIF 1 gene expression. It is possible to conclude that BBR-COSNPs can be used in oral administration formulations for lunganticancer therapy.
International Journal of Halal Research, Dec 1, 2019
International Journal of Pharmaceutics, Sep 1, 2020
Environmental Science and Pollution Research, Aug 15, 2018
Mutation Research, Apr 1, 2005
International Journal of Hygiene and Environmental Health, Jul 1, 2009
Waste and Biomass Valorization, Jun 3, 2023
Comunicata Scientiae, Jun 30, 2014
International Journal of Agricultural Technology, 2013
Toxicology Letters, Aug 1, 2011
Recent Patents on Food, Nutrition & Agriculture, Dec 31, 2021
Molecular Neurobiology, Feb 20, 2016
Physiology and Molecular Biology of Plants, Aug 1, 2021
Mycorrhizal symbiosis is generally considered effective in ameliorating plant tolerance to abioti... more Mycorrhizal symbiosis is generally considered effective in ameliorating plant tolerance to abiotic stress by altering gene expression, and evaluation of genes involved in ion homeostasis and nutrient uptake. This study aimed to use arbuscular mycorrhizal fungus (AMF) to alleviate salinity stress and analyse relevant gene expression in pistachio plants under No/NaCl stress in greenhouse conditions. Arbuscular mycorrhizal symbiosis was used to study the physiological responses, ion distribution and relevant gene expression in pistachio plants under salinity stress. After four months of symbiosis, mycorrhizal root colonization showed a significant reduction in all tested parameters under salt stress treatment compared to non-saline treatment. Salinity affected the morphological traits, and decreased the nutrient content including N, P, Mg and Fe as well as K/Na and Ca/Na ratios, relative water content (RWC), membrane stability index (MSI), and increased the concentration of K, Ca and Na nutrient, glycine betaine, ROS and MDA. Inoculation of seedlings with AMF mitigated the negative effects of salinity on plant growth as indicated by increasing the root colonization, morphological traits, glycine betaine, RWC and MSI. Specifically, under salinity stress, shoot and root dry weight, P and Fe nutrient content, K/Na and Ca/Na ratio of AMF plants were increased by 53.2, 48.6, 71.6, 60.2, 87.5, and 80.1% respectively, in contrast to those of the NMF plants. The contents of Na, O2•− and MDA in AMF plants were significantly decreased by 66.8, 36.8, and 23.1%, respectively at 250 mM NaCl. Moreover, salinity markedly increased SOS1, CCX2 and SKOR genes expression and the inoculation with AMF modulated these genes expression; however, NRT2.4, PHO1 and PIP2.4 gene expressions were increased by salinity and AMF. It could be concluded that inoculation of AMF with Rhizophagus irregularis conferred a larger endurance towards soil salinity in pistachio plants and stimulate the nutrient uptake and ionic homeostasis maintenance, superior RWC and osmoprotection, toxic ion partitioning, maintaining membrane integrity and the ion-relevant genes expression.
Abstract Deoxynivalenol (DON) is a mycotoxin mainly produced by Fusarium spp. It is highly toxic ... more Abstract Deoxynivalenol (DON) is a mycotoxin mainly produced by Fusarium spp. It is highly toxic and widely spread dissemination. DON is considered one of the most important trichothecenes which can generate free radicals, mainly reactive oxygen species (ROS), which enhances lipid peroxidation, reduces the activity of antioxidant enzymes, and eventually leads to apoptosis. Thus, oxidative stress is considered one of the most important mechanisms in DON toxicity. Natural antioxidant agents are considered the most effective to protect against DON for human and animals. In this chapter, we review the molecular mechanisms underlying DON-induced oxidative stress. We also summarized the current literature regarding the silymarin (SIL) and inulin (IN) as antioxidant agents for the protection against DON-induced oxidative stress. This chapter provides an inclusive overview of the mechanism of DON-induced oxidative stress and the mechanisms of antioxidant activity induced by SIL and IN against DON.
Journal of Aging Research, Nov 29, 2019
Soft nanoscience letters, 2015
Archives of Toxicology, Sep 14, 2007
Food and Chemical Toxicology, Jul 1, 2007
Aflatoxins are potent toxins and carcinogens which can be excreted in the milk of exposed lactati... more Aflatoxins are potent toxins and carcinogens which can be excreted in the milk of exposed lactating mothers mainly in the form of aflatoxin M(1) (AFM(1)). We previously evaluated the level and frequency of AFM(1) in breast milk in a group of Egyptian mothers attending the New El-Qalyub Hospital, Qalyubiyah governorate, Egypt. In this study, fifty of those women who were AFM(1) positive were revisited monthly for 12 months to assess the temporal variation in breast milk AFM(1). AFM(1) was detected in 248 of 443 (56%) samples. In a multilevel model of the data there was a highly significant (p<0.001) effect of month of sampling on the frequency of AFM(1) detection with summer months having the highest frequency (>80%) and winter months the lowest frequency (<20%) of detection. AFM(1) was observed most frequently in June [OR 63, 95% CI (7.6, 522)]. The level of AFM(1) detection also followed this seasonal pattern with highest mean level in July (64 pg/ml milk, range 6.3-497 pg/ml milk) and the lowest mean level in January (8 pg/ml milk, range 4.2-108 pg/ml milk). The duration of lactation [p=0.0035, OR=1.08, 95% CI (1.02, 1.13)], and peanut consumption [p=0.06, OR=1.69, 95% CI (0.9, 2.9)] also contributed to the model. The identification and understanding of factors determining the presence of toxicants in human milk is important and may provide a knowledge driven basis for controlling the transfer of chemicals to infants.
International Journal of Halal Research, Dec 1, 2019
International Journal of Pharmaceutics, Apr 1, 2022
Lung cancer is one of the most common types of malignant tumors of the respiratory system and has... more Lung cancer is one of the most common types of malignant tumors of the respiratory system and has the highest rates of incidence and mortality of malignant tumors. This study aimed to synthesize and characterize berberine-loaded chitosan nanoparticles (BBR-COSNPs) and to evaluate their protective effects against urethane-induced lung cancer. Forty male albino mice were divided into four groups, with the first serving as a negative control and the other three groups were injected intraperitoneally with urethane (1 mg/kg b.w) each other day for one week then group 2 was served as a positive control, however, groups 3 and 4 were treated orally with a daily dose of BBR or BBR-COSNPs (75 mg/kg b.w) for 10 consecutive weeks. Blood and lung tissue samples are collected for laboratory assay. The BBR-COSNPs were spherical, with an average particle size of 45.56 nm and zeta potential of 39.82 1.82 mV. The in vivo data demonstrated that mice given urethane alone had a significant increase in MDA, NO, NF-κB level, HIF1-α, and COX-2-positive expression in the lung tissue and serum VEGFR2, ALT, AST, urea, and creatinine accompanied with a significant decrease in GSH, SOD, caspase 9 in the lung tissue and serum BAX. Co-treatment with BBR-COSNPs suppressed lung cancer growth and promoted apoptosis by modulating serum BAX and lung caspase 9 gene expressions. In addition, BBR-COSNPs inhibited tumor angiogenesis by reduction in levels of serum VEGFR2 and lung HIF 1 gene expression. It is possible to conclude that BBR-COSNPs can be used in oral administration formulations for lunganticancer therapy.
International Journal of Halal Research, Dec 1, 2019
International Journal of Pharmaceutics, Sep 1, 2020
Environmental Science and Pollution Research, Aug 15, 2018
Mutation Research, Apr 1, 2005
International Journal of Hygiene and Environmental Health, Jul 1, 2009
Waste and Biomass Valorization, Jun 3, 2023
Comunicata Scientiae, Jun 30, 2014
International Journal of Agricultural Technology, 2013
Toxicology Letters, Aug 1, 2011