sahar elsouda | National Research Centre (original) (raw)

Papers by sahar elsouda

Egyptian Pharmaceutical Journal, 2017

Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The st... more Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The study aimed to investigate the phenolic constituents and evaluate the antioxidant and α-amylase inhibitory activities of the plant’s extracts and its major compounds. Materials and methods The compounds were isolated through chromatographic techniques from the defatted ethanolic extract (DAEE). Their structures were determined by ultraviolet, mass spectrometer, and nuclear magnetic resonance spectroscopy. Results The flavonoids kaempferol 3-O-(6″galloyl)- apiofuranosyl (1‴→2″)-β-galactopyranoside, kaempferol-3-O-β-glucopyranosyl(1‴→6″)-β-glucopyranoside, kaempferol 3-O-(6″galloyl)-apiofuranosyl (1‴→2″)-β-galactopyranoside, rutin, vitexin, isovitexin, orientin, quercetin, kaempferol; the tannins ellagic acid, gallic acid, methyl gallate, 2,6-di-O-galloyl(α/β)glucoside, 2,3-di-O-galloyl(α/β)glucoside, and tetragalloyl glucoside in addition to two benzene acetic acid derivatives, harpulliaside A and cavaol B, were isolated from the total bioactive ethanolic extract (TEE). The TEE and the DAEE of H. pendula have a total phenolic content of 255.5±7.18 and 222.9±6.43 mg gallic acid equivalents/g extract, respectively, and a total flavonoid content of 111.6±3.2 and 102.6±2.6 mg quercetin equivalents/g extract, respectively. With respect to the in vitro study, DAEE, TEE, and methyl gallate showed an interesting inhibitory activity on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) [half maximal inhibitory concentration (IC50): 13.3±0.4, 17.7±0.7, and 19.4±0.08 µg/ml, respectively], nitric oxide (IC50: 12.8±2.54, 18.3±1.6, and 29.8±1.00 µg/ml, respectively), and α-amylase (IC50: 6.1±0.554, 14.4±0.681, and17.5±0.003 µg/ml, respectively). Conclusion H. pendula extracts are rich in phenolic compounds; the aforementioned results suggest that DAEE, TEE, and methyl gallate may be potentially useful in hegemony of obesity and diabetes.

International journal of Pharmacy and Pharmaceutical Sciences, Aug 31, 2014

Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residu... more Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residues. Therefore, the present study is designed to evaluate the petroleum ether extract of Atriplex lindleyi to attenuate the hepato-renal injury induced by BB exposure and study its cytotoxic activity against different human cell line as well as to describe the chemical composition of the petroleum ether extract. Methods: The phytochemical study of petroleum ether extract was implemented using both GC/MS and column chromatography analysis. The isolated compounds were identified using different spectroscopic analysis. Hepato-renal assay, rats were intraperitonealy injected bromobenzene at a dose 460 mg/kg BW. The petroleum ether extract as well as Hepaticum were administrated orally twice a week for three consecutive weeks with a dose 150 & 100 mg/kg body weight, respectively. Liver marker enzymes, liver function indices and kidney function tests were estimated. The cytotoxic activity of, petroleum ether extract was assessed by the mitochondrial dependent reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide). Results: Forty two compounds as well as sixteen fatty acids were identified in unsaponifiable and saponifiable fractions, respectively. Unsaponifiable fraction constituted of hydrocarbons (73.39% of total unsaponifiable matter), alcoholic (0.88%) and steroidal compounds (2.22%). Furthermore, column chromatography of petroleum ether extract afforded nonsterol tritrepenoids; olean-12-en-3,11-dione (1), β-amyrenone (2), erythrodiol I (3), Lupeol (4) as well as sterol triterpenoids; cholesterol (5) and mixture of β-sitosterol and stigmasterol (6). Compounds 1, 3 and 5 are first reported from Atriplex lindleyi. In addition, GC/MS analysis of the main fraction isolated from column chromatography revealed phytol as a major component. Drastic changes were observed after BB intoxication in liver function parameters; kidney disorder indices and certain oxidative stress markers. Treatment with petroleum ether extract improved all biochemical parameters under investigation as well as the histopathology of liver and kidney. Petroleum ether extract showed growth inhibition of HepG2 and MCF7 human cells by 44.8 and 29.9%, respectively at 100 µg/ml. Conclusion: The petroleum ether extract of A. lindleyi contains bioactive compounds exhibiting hepato-renal protection and cytotoxic activity.

Planta Medica, 2013

The microalga Spirulina platensis contains 11.09% w/w of total polysaccharides. The water soluble... more The microalga Spirulina platensis contains 11.09% w/w of total polysaccharides. The water soluble polysaccharides, obtained by cold and hot water extract method, yielded 4.45 and 3.37% w/w, respectively. The total sugar content of cold and hot polysaccharide extracts were 67.29 and 64.66%, respectively. Furthermore according to GC analysis twelve and eleven sugars could be identified in cold and hot extracts, respectively. Glucose, galactose and mannose are predominant sugars in both extracts. The cold and hot water extracts significantly reduced the replication of Hepatitis C Virus (genotype 4) as well as scavenging ability against nitric oxide in concentration-dependent manner. Moreover, the hot extract exhibited cytotoxic activity against HepG2 (human cell line) while the cold extract showed hypolipidemic activity.

The petroleum ether, chloroform, and 70% ethanol ex tracts from leaves of Cassia roxburghii DC., ... more The petroleum ether, chloroform, and 70% ethanol ex tracts from leaves of Cassia roxburghii DC., Were investigated for their antiulcerogenic activity usi ng indomethacin as non steroidal ‐ anti-inflammato ry drug induced ‐ulcer in animal model at a dose 300mg/kg. The histopathological examination of gastric mucosa and duodenum showed potent antiulcerogenic effect for t he petroleum ether extract followed by ethanol extr act. In contrast the chloroform extract showed no gastroduo denal protection. In order to determine the biologi cally active compounds, phytochemical study of the bioactive pet roleum ether extract was achieved by column chromat ography, which allowed isolation of betulin, emodin and aloe modin. Structures of the isolated compounds were el ucidated by spectroscopic analysis. GC/Mass of fatty acids and unsaponifiable matters lead to the identification o f thirteen fatty acids, the unsaturated fatty acids represent (47%) of the total fatty acids. Sterols represent 16% ...

Herba Polonica, 2013

The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. agains... more The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. against HCT-116 and MCF-7 cell lines resulted with IC50=34.9 and 38.04 μg/ml, respectively, while against HepG-2 showed no activity. A bioassay guided-fractionation approach was conducted to isolate and identify the active cytotoxic principles. Further chromatographic separation and purification of the petroleum ether extract resulted in the isolation of two anthraquinones identified as aloe-emodin acetate and aloe-emodin, along with stigmasterol, β-sitosterol and palmitic acid. The structure elucidation of isolated compounds was performend using 1D, 2D-NMR and HR-MS. Furthermore, the cytotoxicity of aloe-emodin acetate and aloe-emodin were evaluated and resulted with IC50=153.30 and 70.02 μg/ml against HCT-116 and with 93.20 and 53.20 μg/ml against MCF-7, respectively, while against HepG-2 showed no activity. Moreover, the antiviral activity of the two isolated anthraquinones was tested again...

Scientific Journal of October 6 University, 2014

Egyptian Pharmaceutical Journal, 2017

Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The st... more Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The study aimed to investigate the phenolic constituents and evaluate the antioxidant and α-amylase inhibitory activities of the plant’s extracts and its major compounds. Materials and methods The compounds were isolated through chromatographic techniques from the defatted ethanolic extract (DAEE). Their structures were determined by ultraviolet, mass spectrometer, and nuclear magnetic resonance spectroscopy. Results The flavonoids kaempferol 3-O-(6″galloyl)- apiofuranosyl (1‴→2″)-β-galactopyranoside, kaempferol-3-O-β-glucopyranosyl(1‴→6″)-β-glucopyranoside, kaempferol 3-O-(6″galloyl)-apiofuranosyl (1‴→2″)-β-galactopyranoside, rutin, vitexin, isovitexin, orientin, quercetin, kaempferol; the tannins ellagic acid, gallic acid, methyl gallate, 2,6-di-O-galloyl(α/β)glucoside, 2,3-di-O-galloyl(α/β)glucoside, and tetragalloyl glucoside in addition to two benzene acetic acid derivatives, harpulliaside A and cavaol B, were isolated from the total bioactive ethanolic extract (TEE). The TEE and the DAEE of H. pendula have a total phenolic content of 255.5±7.18 and 222.9±6.43 mg gallic acid equivalents/g extract, respectively, and a total flavonoid content of 111.6±3.2 and 102.6±2.6 mg quercetin equivalents/g extract, respectively. With respect to the in vitro study, DAEE, TEE, and methyl gallate showed an interesting inhibitory activity on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) [half maximal inhibitory concentration (IC50): 13.3±0.4, 17.7±0.7, and 19.4±0.08 µg/ml, respectively], nitric oxide (IC50: 12.8±2.54, 18.3±1.6, and 29.8±1.00 µg/ml, respectively), and α-amylase (IC50: 6.1±0.554, 14.4±0.681, and17.5±0.003 µg/ml, respectively). Conclusion H. pendula extracts are rich in phenolic compounds; the aforementioned results suggest that DAEE, TEE, and methyl gallate may be potentially useful in hegemony of obesity and diabetes.

Journal of Herbs, Spices & Medicinal Plants, 2015

ABSTRACT The antiulcer effect of Matricaria chamomilla flower head extracts were investigated on ... more ABSTRACT The antiulcer effect of Matricaria chamomilla flower head extracts were investigated on indomethacin-induced ulcer models in rats. All extracts exhibited antiulcer effect; the highest percent of inhibition was shown by the ethyl acetate and chloroform fractions (84, 80%) respectively, followed by petroleum ether and aqueous ethanol residue (57, 50%), respectively, while the total alcoholic extract showed 62%. Phytochemical investigation of the ethyl acetate fraction allowed isolation and identification of apigenin-7-methoxy -8-O-arabinopyranoside, apigenin -7-O-β-glucoside - 6″ acetate, apigenin-7-O-galactoside - 6″ acetate, and apigenin -7-O-β-glucoside, by NMR, mass analysis, and acid hydrolysis.

Asian Pacific Journal of Tropical Disease, 2014

Zeitschrift für Naturforschung C, 2012

Carbohydrate Research, 2012

Journal of Herbs, Spices & Medicinal Plants, 2015

Antioxidant, antimicrobial activities of flavonoids glycoside from Leucaena leucocephala leaves

Anales De La Real Academia Nacional De Farmacia, 2015

Characterization of flavonoids and limonoids in the defatted acetone extract of Khaya senegalensi... more Characterization of flavonoids and limonoids in the defatted acetone extract of Khaya senegalensis flowers (A. Juss.) contents was performed using ultra performance liquid chromatography (UPLC) coupled with ultraviolet (UV) and electrospray ionization (ESI) mass spectrometry, furthermore, tandem mass spectrometry (MS/MS) was performed to assist in the structural elucidation. The antimicrobial effect was tested against representative gram positive and negative bacteria and candida. Cytotoxicity of extract was evaluated using the mitochondrial-dependent reduction of MTT. The method used enabled identification of five flavonoid glycosides (di and mono-sugar) and twelve limonoids of different types viz : mexicanolides, phragmalins and angolensate were tentatively identified. The extract was effective against tested microorganism revealing potent growth inhibitory effect on Salmonella typhimurium ATCC 25566, Escherichia coli NRRN 3008 , Pseudomonas aeruginosa ATCC 10145 and fungus Candi...

International Journal of Pharmacy and Pharmaceutical Sciences, 2014

Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residu... more Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residues. Therefore, the present study is designed to evaluate the petroleum ether extract of Atriplex lindleyi to attenuate the hepato-renal injury induced by BB exposure and study its cytotoxic activity against different human cell line as well as to describe the chemical composition of the petroleum ether extract. Methods : The phytochemical study of petroleum ether extract was implemented using both GC/MS and column chromatography analysis. The isolated compounds were identified using different spectroscopic analysis. Hepato-renal assay, rats were intraperitonealy injected bromobenzene at a dose 460 mg/kg BW. The petroleum ether extract as well as Hepaticum were administrated orally twice a week for three consecutive weeks with a dose 150 & 100 mg/kg body weight, respectively. Liver marker enzymes, liver function indices and kidney function tests were estimated. The cytotoxic activity of, ...

Drug development and industrial pharmacy, Jan 21, 2017

Bile salts containing vesicles (bilosomes) represent a portentous vesicular carrier that showed p... more Bile salts containing vesicles (bilosomes) represent a portentous vesicular carrier that showed prosperous results in delivering active moieties in the gastrointestinal tract (GIT). In this study, bilosomes were exploited to deliver sulfated polysaccharide-protein complexes of Enteromorpha intestinalis (EHEM) and enhance its activity against hepatocellular carcinoma as well as resist harsh GIT conditions. Bilosomes were prepared using the sodium salt of three different bile acids (cholic, deoxycholic, taurodeoxycholic) and two different nonionic surfactants (Span 40 and 65). The effects of experimental variables were thoroughly studied to obtain an optimum formulation loading EHEM. The selected formulation (EH-Bilo-2) prepared with sodium cholate and Span 65 displayed nano-sized (181.1 ± 16.80 nm) spherical vesicles with reasonable entrapment efficiency (71.60 ± 0.25%) and controlled release properties; and thus was investigated as anti-hepatocarcinogenic candidate for in vivo studi...

Journal of Applied Pharmaceutical Science, 2015

Egyptian Pharmaceutical Journal, 2017

Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The st... more Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The study aimed to investigate the phenolic constituents and evaluate the antioxidant and α-amylase inhibitory activities of the plant’s extracts and its major compounds. Materials and methods The compounds were isolated through chromatographic techniques from the defatted ethanolic extract (DAEE). Their structures were determined by ultraviolet, mass spectrometer, and nuclear magnetic resonance spectroscopy. Results The flavonoids kaempferol 3-O-(6″galloyl)- apiofuranosyl (1‴→2″)-β-galactopyranoside, kaempferol-3-O-β-glucopyranosyl(1‴→6″)-β-glucopyranoside, kaempferol 3-O-(6″galloyl)-apiofuranosyl (1‴→2″)-β-galactopyranoside, rutin, vitexin, isovitexin, orientin, quercetin, kaempferol; the tannins ellagic acid, gallic acid, methyl gallate, 2,6-di-O-galloyl(α/β)glucoside, 2,3-di-O-galloyl(α/β)glucoside, and tetragalloyl glucoside in addition to two benzene acetic acid derivatives, harpulliaside A and cavaol B, were isolated from the total bioactive ethanolic extract (TEE). The TEE and the DAEE of H. pendula have a total phenolic content of 255.5±7.18 and 222.9±6.43 mg gallic acid equivalents/g extract, respectively, and a total flavonoid content of 111.6±3.2 and 102.6±2.6 mg quercetin equivalents/g extract, respectively. With respect to the in vitro study, DAEE, TEE, and methyl gallate showed an interesting inhibitory activity on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) [half maximal inhibitory concentration (IC50): 13.3±0.4, 17.7±0.7, and 19.4±0.08 µg/ml, respectively], nitric oxide (IC50: 12.8±2.54, 18.3±1.6, and 29.8±1.00 µg/ml, respectively), and α-amylase (IC50: 6.1±0.554, 14.4±0.681, and17.5±0.003 µg/ml, respectively). Conclusion H. pendula extracts are rich in phenolic compounds; the aforementioned results suggest that DAEE, TEE, and methyl gallate may be potentially useful in hegemony of obesity and diabetes.

International journal of Pharmacy and Pharmaceutical Sciences, Aug 31, 2014

Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residu... more Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residues. Therefore, the present study is designed to evaluate the petroleum ether extract of Atriplex lindleyi to attenuate the hepato-renal injury induced by BB exposure and study its cytotoxic activity against different human cell line as well as to describe the chemical composition of the petroleum ether extract. Methods: The phytochemical study of petroleum ether extract was implemented using both GC/MS and column chromatography analysis. The isolated compounds were identified using different spectroscopic analysis. Hepato-renal assay, rats were intraperitonealy injected bromobenzene at a dose 460 mg/kg BW. The petroleum ether extract as well as Hepaticum were administrated orally twice a week for three consecutive weeks with a dose 150 & 100 mg/kg body weight, respectively. Liver marker enzymes, liver function indices and kidney function tests were estimated. The cytotoxic activity of, petroleum ether extract was assessed by the mitochondrial dependent reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide). Results: Forty two compounds as well as sixteen fatty acids were identified in unsaponifiable and saponifiable fractions, respectively. Unsaponifiable fraction constituted of hydrocarbons (73.39% of total unsaponifiable matter), alcoholic (0.88%) and steroidal compounds (2.22%). Furthermore, column chromatography of petroleum ether extract afforded nonsterol tritrepenoids; olean-12-en-3,11-dione (1), β-amyrenone (2), erythrodiol I (3), Lupeol (4) as well as sterol triterpenoids; cholesterol (5) and mixture of β-sitosterol and stigmasterol (6). Compounds 1, 3 and 5 are first reported from Atriplex lindleyi. In addition, GC/MS analysis of the main fraction isolated from column chromatography revealed phytol as a major component. Drastic changes were observed after BB intoxication in liver function parameters; kidney disorder indices and certain oxidative stress markers. Treatment with petroleum ether extract improved all biochemical parameters under investigation as well as the histopathology of liver and kidney. Petroleum ether extract showed growth inhibition of HepG2 and MCF7 human cells by 44.8 and 29.9%, respectively at 100 µg/ml. Conclusion: The petroleum ether extract of A. lindleyi contains bioactive compounds exhibiting hepato-renal protection and cytotoxic activity.

Planta Medica, 2013

The microalga Spirulina platensis contains 11.09% w/w of total polysaccharides. The water soluble... more The microalga Spirulina platensis contains 11.09% w/w of total polysaccharides. The water soluble polysaccharides, obtained by cold and hot water extract method, yielded 4.45 and 3.37% w/w, respectively. The total sugar content of cold and hot polysaccharide extracts were 67.29 and 64.66%, respectively. Furthermore according to GC analysis twelve and eleven sugars could be identified in cold and hot extracts, respectively. Glucose, galactose and mannose are predominant sugars in both extracts. The cold and hot water extracts significantly reduced the replication of Hepatitis C Virus (genotype 4) as well as scavenging ability against nitric oxide in concentration-dependent manner. Moreover, the hot extract exhibited cytotoxic activity against HepG2 (human cell line) while the cold extract showed hypolipidemic activity.

The petroleum ether, chloroform, and 70% ethanol ex tracts from leaves of Cassia roxburghii DC., ... more The petroleum ether, chloroform, and 70% ethanol ex tracts from leaves of Cassia roxburghii DC., Were investigated for their antiulcerogenic activity usi ng indomethacin as non steroidal ‐ anti-inflammato ry drug induced ‐ulcer in animal model at a dose 300mg/kg. The histopathological examination of gastric mucosa and duodenum showed potent antiulcerogenic effect for t he petroleum ether extract followed by ethanol extr act. In contrast the chloroform extract showed no gastroduo denal protection. In order to determine the biologi cally active compounds, phytochemical study of the bioactive pet roleum ether extract was achieved by column chromat ography, which allowed isolation of betulin, emodin and aloe modin. Structures of the isolated compounds were el ucidated by spectroscopic analysis. GC/Mass of fatty acids and unsaponifiable matters lead to the identification o f thirteen fatty acids, the unsaturated fatty acids represent (47%) of the total fatty acids. Sterols represent 16% ...

Herba Polonica, 2013

The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. agains... more The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. against HCT-116 and MCF-7 cell lines resulted with IC50=34.9 and 38.04 μg/ml, respectively, while against HepG-2 showed no activity. A bioassay guided-fractionation approach was conducted to isolate and identify the active cytotoxic principles. Further chromatographic separation and purification of the petroleum ether extract resulted in the isolation of two anthraquinones identified as aloe-emodin acetate and aloe-emodin, along with stigmasterol, β-sitosterol and palmitic acid. The structure elucidation of isolated compounds was performend using 1D, 2D-NMR and HR-MS. Furthermore, the cytotoxicity of aloe-emodin acetate and aloe-emodin were evaluated and resulted with IC50=153.30 and 70.02 μg/ml against HCT-116 and with 93.20 and 53.20 μg/ml against MCF-7, respectively, while against HepG-2 showed no activity. Moreover, the antiviral activity of the two isolated anthraquinones was tested again...

Scientific Journal of October 6 University, 2014

Egyptian Pharmaceutical Journal, 2017

Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The st... more Background and objective Harpullia pendula Planch leaves belong to the Sapindaceae family. The study aimed to investigate the phenolic constituents and evaluate the antioxidant and α-amylase inhibitory activities of the plant’s extracts and its major compounds. Materials and methods The compounds were isolated through chromatographic techniques from the defatted ethanolic extract (DAEE). Their structures were determined by ultraviolet, mass spectrometer, and nuclear magnetic resonance spectroscopy. Results The flavonoids kaempferol 3-O-(6″galloyl)- apiofuranosyl (1‴→2″)-β-galactopyranoside, kaempferol-3-O-β-glucopyranosyl(1‴→6″)-β-glucopyranoside, kaempferol 3-O-(6″galloyl)-apiofuranosyl (1‴→2″)-β-galactopyranoside, rutin, vitexin, isovitexin, orientin, quercetin, kaempferol; the tannins ellagic acid, gallic acid, methyl gallate, 2,6-di-O-galloyl(α/β)glucoside, 2,3-di-O-galloyl(α/β)glucoside, and tetragalloyl glucoside in addition to two benzene acetic acid derivatives, harpulliaside A and cavaol B, were isolated from the total bioactive ethanolic extract (TEE). The TEE and the DAEE of H. pendula have a total phenolic content of 255.5±7.18 and 222.9±6.43 mg gallic acid equivalents/g extract, respectively, and a total flavonoid content of 111.6±3.2 and 102.6±2.6 mg quercetin equivalents/g extract, respectively. With respect to the in vitro study, DAEE, TEE, and methyl gallate showed an interesting inhibitory activity on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) [half maximal inhibitory concentration (IC50): 13.3±0.4, 17.7±0.7, and 19.4±0.08 µg/ml, respectively], nitric oxide (IC50: 12.8±2.54, 18.3±1.6, and 29.8±1.00 µg/ml, respectively), and α-amylase (IC50: 6.1±0.554, 14.4±0.681, and17.5±0.003 µg/ml, respectively). Conclusion H. pendula extracts are rich in phenolic compounds; the aforementioned results suggest that DAEE, TEE, and methyl gallate may be potentially useful in hegemony of obesity and diabetes.

Journal of Herbs, Spices & Medicinal Plants, 2015

ABSTRACT The antiulcer effect of Matricaria chamomilla flower head extracts were investigated on ... more ABSTRACT The antiulcer effect of Matricaria chamomilla flower head extracts were investigated on indomethacin-induced ulcer models in rats. All extracts exhibited antiulcer effect; the highest percent of inhibition was shown by the ethyl acetate and chloroform fractions (84, 80%) respectively, followed by petroleum ether and aqueous ethanol residue (57, 50%), respectively, while the total alcoholic extract showed 62%. Phytochemical investigation of the ethyl acetate fraction allowed isolation and identification of apigenin-7-methoxy -8-O-arabinopyranoside, apigenin -7-O-β-glucoside - 6″ acetate, apigenin-7-O-galactoside - 6″ acetate, and apigenin -7-O-β-glucoside, by NMR, mass analysis, and acid hydrolysis.

Asian Pacific Journal of Tropical Disease, 2014

Zeitschrift für Naturforschung C, 2012

Carbohydrate Research, 2012

Journal of Herbs, Spices & Medicinal Plants, 2015

Antioxidant, antimicrobial activities of flavonoids glycoside from Leucaena leucocephala leaves

Anales De La Real Academia Nacional De Farmacia, 2015

Characterization of flavonoids and limonoids in the defatted acetone extract of Khaya senegalensi... more Characterization of flavonoids and limonoids in the defatted acetone extract of Khaya senegalensis flowers (A. Juss.) contents was performed using ultra performance liquid chromatography (UPLC) coupled with ultraviolet (UV) and electrospray ionization (ESI) mass spectrometry, furthermore, tandem mass spectrometry (MS/MS) was performed to assist in the structural elucidation. The antimicrobial effect was tested against representative gram positive and negative bacteria and candida. Cytotoxicity of extract was evaluated using the mitochondrial-dependent reduction of MTT. The method used enabled identification of five flavonoid glycosides (di and mono-sugar) and twelve limonoids of different types viz : mexicanolides, phragmalins and angolensate were tentatively identified. The extract was effective against tested microorganism revealing potent growth inhibitory effect on Salmonella typhimurium ATCC 25566, Escherichia coli NRRN 3008 , Pseudomonas aeruginosa ATCC 10145 and fungus Candi...

International Journal of Pharmacy and Pharmaceutical Sciences, 2014

Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residu... more Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residues. Therefore, the present study is designed to evaluate the petroleum ether extract of Atriplex lindleyi to attenuate the hepato-renal injury induced by BB exposure and study its cytotoxic activity against different human cell line as well as to describe the chemical composition of the petroleum ether extract. Methods : The phytochemical study of petroleum ether extract was implemented using both GC/MS and column chromatography analysis. The isolated compounds were identified using different spectroscopic analysis. Hepato-renal assay, rats were intraperitonealy injected bromobenzene at a dose 460 mg/kg BW. The petroleum ether extract as well as Hepaticum were administrated orally twice a week for three consecutive weeks with a dose 150 & 100 mg/kg body weight, respectively. Liver marker enzymes, liver function indices and kidney function tests were estimated. The cytotoxic activity of, ...

Drug development and industrial pharmacy, Jan 21, 2017

Bile salts containing vesicles (bilosomes) represent a portentous vesicular carrier that showed p... more Bile salts containing vesicles (bilosomes) represent a portentous vesicular carrier that showed prosperous results in delivering active moieties in the gastrointestinal tract (GIT). In this study, bilosomes were exploited to deliver sulfated polysaccharide-protein complexes of Enteromorpha intestinalis (EHEM) and enhance its activity against hepatocellular carcinoma as well as resist harsh GIT conditions. Bilosomes were prepared using the sodium salt of three different bile acids (cholic, deoxycholic, taurodeoxycholic) and two different nonionic surfactants (Span 40 and 65). The effects of experimental variables were thoroughly studied to obtain an optimum formulation loading EHEM. The selected formulation (EH-Bilo-2) prepared with sodium cholate and Span 65 displayed nano-sized (181.1 ± 16.80 nm) spherical vesicles with reasonable entrapment efficiency (71.60 ± 0.25%) and controlled release properties; and thus was investigated as anti-hepatocarcinogenic candidate for in vivo studi...

Journal of Applied Pharmaceutical Science, 2015