Beata Sarosiek | Institute of Animal Reproduction and Food Research (original) (raw)
Papers by Beata Sarosiek
Animal reproduction science, Jan 17, 2018
This study investigated how the inhibition of certain enzymes present in ide sperm influences spe... more This study investigated how the inhibition of certain enzymes present in ide sperm influences sperm motility and the percentage of fertilised eggs. The enzymes studied were acid phosphatase (AcP), β-N-acetylglucosaminidase (β-NAGase) and lactate dehydrogenase (LDH). None of the inhibitors affected ide sperm motility parameters. The addition of gossypol (a LDH inhibitor) caused a considerable increase in the percentage of fertilised eggs (92-95% compared to 63% in the control). The inhibition of AcP caused a considerable decrease in fertility rate - at the highest inhibitor dose, the percentage of fertilised eggs decreased to 26%. A similar effect was seen after the addition of acetamide (a β-NAGase inhibitor), but in this case the highest dose caused complete inhibition of fertilisation. The results presented here indicate the importance of AcP and β-NAGase in the process of ide fertilisation.
Animal reproduction science, 2018
Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L... more Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage. Semen quantity (semen volume and sperm count), quality (sperm motility and sperm viability), as well as seminal plasma parameters (pH of seminal plasma and seminal plasma osmotic pressure) and its enzymatic activity, e.g., lactate dehydrogenase (LDH) and ß-N-acetylglucosaminidase (ß-NAG) were determined. Moreover, for the first time, the percentage of live, dead, and apoptotic sperm, as well as the proteolytic activity of seminal plasma, were determined using flow cytometry and zymography, respectively, at specific times during the common carp reproductive period. The lowest volumes of semen and sperm concentration were noted during the first semen collection (June 1). Anal...
Polish Journal of Natural Science
A Computer Assisted Sperm Analysis system, CASA, enables determination of numerous parameters cha... more A Computer Assisted Sperm Analysis system, CASA, enables determination of numerous parameters characterizing sperm motion activity. This system allows for the examination of the effect of various environmental factors on spermatozoa motility parameters. The aim of this work was to compare time-dependent motility changes of sperm obtained from the dace, Leuciscus leuciscus (L.), by abdominal massage (sperm from spermatic ducts) and directly from gonads (testicular sperm). The analysis concerned such sperm motility parameters as: percentage of motile sperm (MOT, %), total sperm velocity (VAP, μm s–1), straight line velocity (VSL, μm s–1), curvilinear velocity (VCL, μm s–1), linearity (LIN: VSL/VCL · 100%), straightness (STR: VSL/VAP · 100%), amplitude of the lateral head displacement (ALH, μm) and beat cross frequency (BCF, Hz). During 105 seconds of motility, no significant differences were found in the values of MOT between sperm originating from the spermatic ducts and from the tes...
Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through ... more Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in samples of stripped and testicular sperm obtained from XY and YY males, and testicular sperm of XX males. The motile spermatozoa, as measured by both subjective method and CASA, showed no differences between testicular sperm of XX males and stripped sperm of XY and YY males whereas testicular sperm of XY and YY males had significantly lower sperm motility. Result of protein densitometry showed similarities in protein profile between seminal plasma of XY and YY males and testicular fluids of XX males. Testis of XX males showed specific histological structures of cysts consists hypertrophied Se...
Journal of the World Aquaculture Society, 2013
ABSTRACT The common carp, Cyprinus carpio L. sperm motility parameters were analyzed by using com... more ABSTRACT The common carp, Cyprinus carpio L. sperm motility parameters were analyzed by using computer-assisted sperm analysis system. The percentage of motile sperm (MOT, %), progressively motile sperm (PRG, %), curvilinear velocity (VCL, μm/sec), average path velocity (VAP, μm/sec), the wobbling index (WOB, %), movement linearity (LIN, %), beat cross frequency (BCF, Hz), and amplitude of lateral head displacement (ALH, μm) were determined. Five activation solutions (As) were used to activate sperm movement. As 1 solution: 68 mM NaCl, 50 mM urea, 0.5% bovine serum albumin (BSA), pH: 7.7, 181 mOsm/kg; As 2 buffer: 100 mM NaCl, 10 mM Tris, 0.5% BSA, pH: 9.0, 199 mOsm/kg; As 3 solution: 86 mM NaCl, 0.5% BSA, pH: 7.4, 167 mOsm/kg; As 4 buffer: 5 mM KCl, 45 mM NaCl, 30 mM Tris, 0.5%, pH: 8.0, 160 mOsm/kg; and As 5 solution: distilled water with the addition of 0.5% BSA, pH: 7.3, <3 mOsm/kg. Among five tested solutions, a buffer with a pH of 9.0 and osmolality of approximately 200 mOsm/kg (As 2) was the most suitable. After its activation, a significant increase in MOT and ALH values was observed, which can be of importance to the effectiveness of egg fertilization.
1 Organisme de formation enregistré sous le numéro 41570143657 auprès du Préfet de la Région Lorr... more 1 Organisme de formation enregistré sous le numéro 41570143657 auprès du Préfet de la Région Lorraine Conférence ELEVAGE DE LA LOTTE (Lota lota) : Recherche et perspectives Jeudi 1 juillet 2004 au Muséum-aquarium de Nancy 34, rue Ste Catherine 54 000 NANCY Tel : 03 83 32 99 97, Fax :03 83 32 30 16 En collaboration avec le Laboratoire de Sciences Animales de L'Université Henri Poincaré Nancy I. Organisé par : Elevage de la lotte (Lota lota)/FLAC/Nancy 2004 _______________________________________________________________________________ Filière Lorraine d'Aquaculture Continentale -67, rue principale -57 260 Lindre-Basse Tel : 03 87 86 07 23 Fax : 03 87 86 07 85
Reproductive Biology, Feb 1, 2006
Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in t... more Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in the environment have adverse effects on the reproductive function in fish. Three studies were conducted to assess toxicity of these chemicals towards reproduction of freshwater fish. It was shown that heavy metals added to the diets accumulate in brain tissue of carp, and this accumulation results in inhibition of the secretion of noradrenaline and stimulation of the secretion of dopamine in the hypothalamus. These processes results in a disturbance of hormonal equilibrium of the hypothalamo-pituitary system, which can unfavorably influence the efficiency of artificial spawning in fish. Quality of salmonid and sturgeon sperm was impaired after in vitro exposure to heavy metals. The degree of this toxic effect was species-specific.
Polish Journal of Natural Science
Acid phosphatase (AcP) is a commonly observed enzyme in animal semen. In this study, AcP in rainb... more Acid phosphatase (AcP) is a commonly observed enzyme in animal semen. In this study, AcP in rainbow trout (Oncorhynchus mykiss) spermatozoa was partly purified and characterized. Extraction in 0.85% NaCl with 0.1% Triton X-100 enabled obtaining 95% of total AcP activity observed in sperm supernatant. Kinetic characteristics were described for the enzyme from sperm extract and for the partly purified
The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthe... more The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) was frozen without cryoprotector (at -79C) or cryopreserved with methanol as the cryoprotector. The activity of arylsulfatase (AS), acid phosphatase (AcP), -N-acetylglucosaminidase (NAGase), and protein concentration was determined. The protein concentration and enzymatic activities in supernatant obtained after cryopreservation were higher than in milt plasma, but
Journal of Applied Ichthyology, 2015
ABSTRACT Acid phosphatase and arylsulphatase are present in the semen of many animal species, inc... more ABSTRACT Acid phosphatase and arylsulphatase are present in the semen of many animal species, including fish. The presence of these two enzymes has also been confirmed in the semen of Siberian sturgeon (Acipenser baerii). The aim of this study was therefore to determine the potential role of acid phosphatase and arylosulphatase in the fertilization process. Tested were the additions of ammonium molybdate (acid phosphatase inhibitor) and AgNO3 (arylsulphatase inhibitor) regarding their effects on the sperm motility spermatozoa rate and the fertilization rate (in two inhibiting variants: enzymes present in spermatozoa, sperm treated; enzymes present in spermatozoa and eggs, sperm + eggs treated). There was no significant deterioration in motility parameters with either of the applied inhibitors. Ammonium molybdate caused a significant decrease in the fertilization rate (sperm + treated eggs from 86.1 to 9.5%); AgNO3 added only to spermatozoa resulted in a complete inhibition of fertilization. Results indicate that the acid phosphatase activity in eggs and arylsuphatase activity in sperm are necessary for the fertilization process in the Siberian sturgeon.
Journal of Applied Ichthyology, 2015
ABSTRACT Compared were the volume and quality of carp Cyprinus carpio L. obtained from semen of m... more ABSTRACT Compared were the volume and quality of carp Cyprinus carpio L. obtained from semen of males not subjected to hormonal stimulation (N = 10) but injected with 0.9% NaCl in the amount of 1 ml kg -1 b.w., as well as those stimulated using Ovopel (N = 10) in the amount of 1 granule kg -1 b.w. Semen was collected from the males 24 h after injection of hormonal preparation, to determine the pH, total volume (TVS) and volume recalculated per kg of body mass, concentration and total sperm production (TSP). Differences in sperm motility parameters between fish groups were determined by a computer-assisted sperm analysis (CASA) system. Sperm motility was activated using 10 mM Tris buffer, containing 100 mM NaCl (pH 9.0, osmolality 200 mOsm kg -1). The pH, osmolality, total protein concentration and activity of acidic phosphatase (AcP), lactate dehydrogenase (LDH), ß-N-acetylglucosaminidase and arylsulfatase (AS) were determined in the seminal plasma of both test groups. The stimulation of male carp using Ovopel did not significantly affect CASA parameters, but did lead to an over ten-fold increase in the values of TVS and VOS and nearly six times higher values of TSP as compared to the Control group. A significant increase in pH following stimulation with Ovopel indicates that cyclic AMP increased, thanks to which sperm acquire a capacity to move during their passage through the sperm ducts. An increase in the values of CASA parameters is a likely reflection of these changes.
Aquaculture Research, 2015
Aquaculture, 2015
Collection of fish milt by stripping risks contamination with urine, which may negatively influen... more Collection of fish milt by stripping risks contamination with urine, which may negatively influence milt characteristics and quality. Urine contamination of milt may be avoided by using a catheter for sperm collection. The objective of this study was to compare basic pikeperch milt parameters, i.e., sperm concentration (SC), pH, osmolality of seminal plasma (OSM), and motility parameters of spermatozoa analyzed with a CASA system (Computer Assisted Sperm Analysis), collected either with a syringe (as the standard method of sperm collection) or with a catheter. Every parameter of pikeperch milt collected with a catheter was significantly higher (P b 0.05; SC = 12.31 ± 3.81 × 10 9 ml −1 , pH = 9.04 ± 0.07, OSM = 311 ± 16 mOsm kg −1 ) than that collected with a syringe (SC = 8.05 ± 3.04 × 10 9 ml −1 , pH = 8.93 ± 0.09, OSM = 228 ± 51 mOsm kg −1 ), which clearly suggests urine contamination of milt collected with the standard procedure. Moreover, milt collected with catheter was characterized by higher spermatozoa motility parameters, e.g., motility rate of sperm collected with a catheter was 73% whereas the motility rate of sperm collected with a syringe did not exceed 35%. The results obtained in this work indicate that the milt collected with the standard procedure is likely to be exposed to contamination with urine, which may cause a significant reduction of quality parameters. Collection of sperm with a catheter was proven to effectively reduce the contamination of sperm with urine. Therefore, it is recommended to collect pikeperch sperm using a catheter.
Journal of Applied Ichthyology, 2015
ABSTRACT The aim of the study was to investigate the effect of osmolality of the activating solut... more ABSTRACT The aim of the study was to investigate the effect of osmolality of the activating solution (AS) on in vitro fertilization of the eggs of common carp, Cyprinus carpio L. Four different AS (NaCl solutions) were used with different osmolalities: AS-0 (distilled water), AS-100 (100 mOsm kg -1), AS-200 (200 mOsm kg -1) and AS-300 (300 mOsm kg -1). Fertilization was performed in Petri dishes using freshly collected sperm. Insemination (sperm addition to activated eggs) was preceded by exposure of egg samples to each AS for 0 (control group), 15, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 s. Embryonic survival was determined 24 h after incubation at 20°C. Additionally, motility parameters (motility rate [MOT, %] and curvilinear velocity [VCL, lm s -1]) of the spermatozoa activated with the tested AS were measured using a CASA (computer-assisted sperm analysis) system. Motility duration (DUR, s) in each AS was also determined. The results revealed a significant effect of osmolality of the AS on the fertilizing capacity of common carp eggs. There were no viable embryos in the group AS-300. In the group AS-0, the eggs lost their ability for fertilization after 90 s; in the remaining groups, eggs could be fertilized for up to 150 s (group AS-100) or 360 s (group AS-200). Fertilization failure in the group AS-300 was caused by the lack of spermatozoa activation in this solution. The present study findings for in vitro common carp fertilization indicate that special attention should be given to the AS osmolality, where 100 mOsm kg -1 was found to be the most suitable.
Polish Journal of Natural Science, 2007
... JONÓW RTĘCI I KADMU NA AKTYWNOŚĆ WYBRANYCH ENZYMÓW W NASIENIU JESIOTRA SYBERYJSKIEGO (ACIPENS... more ... JONÓW RTĘCI I KADMU NA AKTYWNOŚĆ WYBRANYCH ENZYMÓW W NASIENIU JESIOTRA SYBERYJSKIEGO (ACIPENSER BAERI BRANDT 1869) Beata Sarosiek1, Grzegorz Zdanio2,Radosław Kajetan Kowalski1, Jan Glogowski1 1 Instytut Rozrodu Zwierząt i Badań ...
Reproductive biology, 2006
Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in t... more Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in the environment have adverse effects on the reproductive function in fish. Three studies were conducted to assess toxicity of these chemicals towards reproduction of freshwater fish. It was shown that heavy metals added to the diets accumulate in brain tissue of carp, and this accumulation results in inhibition of the secretion of noradrenaline and stimulation of the secretion of dopamine in the hypothalamus. These processes results in a disturbance of hormonal equilibrium of the hypothalamo-pituitary system, which can unfavorably influence the efficiency of artificial spawning in fish. Quality of salmonid and sturgeon sperm was impaired after in vitro exposure to heavy metals. The degree of this toxic effect was species-specific. It was demonstrated that sperm motility parameters appeared to be good indicators of adverse effects of heavy metals fish sperm. The protection role of seminal ...
Archives of Polish Fisheries, 2008
The aim of the study was to determine the basic quality parameters and selected biochemical marke... more The aim of the study was to determine the basic quality parameters and selected biochemical markers of asp, Aspius aspius (L.), semen after spermiation was stimulated with Ovaprim and Ovopel. Sperm motility and concentration, osmotic pressure, total protein content, and activities of acid phosphatase (AcP), lactate dehydrogenase (LDH), and b-N-acetylglucosaminidase (b-NAG) were determined. It was revealed that higher sperm motility and concentration and higher seminal plasma protein content were obtained after stimulation with Ovaprim. The osmotic pressure of the seminal plasma estimated for males following the administration of Ovopel was higher than after they had been treated with Ovaprim. It was determined that enzyme activity in the seminal plasma of the fish stimulated with Ovaprim was higher in comparison with results obtained after they had been treated with Ovopel. Significant, positive dependencies were confirmed between the concentration of sperm and the total protein content in the seminal plasma (r 2 = 0.492) and the activity of b-NAG (r 2 = 0.779); among the total protein content in the seminal plasma and the activities of AcP (r 2 = 0.476), LDH (r 2 = 0.564), and b-NAG (r 2 = 0.738); and between the activities of AcP and LDH (r 2 = 0.483) and between the activities of LDH and b-NAG (r 2 = 0.844).
Animal reproduction science, Jan 17, 2018
This study investigated how the inhibition of certain enzymes present in ide sperm influences spe... more This study investigated how the inhibition of certain enzymes present in ide sperm influences sperm motility and the percentage of fertilised eggs. The enzymes studied were acid phosphatase (AcP), β-N-acetylglucosaminidase (β-NAGase) and lactate dehydrogenase (LDH). None of the inhibitors affected ide sperm motility parameters. The addition of gossypol (a LDH inhibitor) caused a considerable increase in the percentage of fertilised eggs (92-95% compared to 63% in the control). The inhibition of AcP caused a considerable decrease in fertility rate - at the highest inhibitor dose, the percentage of fertilised eggs decreased to 26%. A similar effect was seen after the addition of acetamide (a β-NAGase inhibitor), but in this case the highest dose caused complete inhibition of fertilisation. The results presented here indicate the importance of AcP and β-NAGase in the process of ide fertilisation.
Animal reproduction science, 2018
Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L... more Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage. Semen quantity (semen volume and sperm count), quality (sperm motility and sperm viability), as well as seminal plasma parameters (pH of seminal plasma and seminal plasma osmotic pressure) and its enzymatic activity, e.g., lactate dehydrogenase (LDH) and ß-N-acetylglucosaminidase (ß-NAG) were determined. Moreover, for the first time, the percentage of live, dead, and apoptotic sperm, as well as the proteolytic activity of seminal plasma, were determined using flow cytometry and zymography, respectively, at specific times during the common carp reproductive period. The lowest volumes of semen and sperm concentration were noted during the first semen collection (June 1). Anal...
Polish Journal of Natural Science
A Computer Assisted Sperm Analysis system, CASA, enables determination of numerous parameters cha... more A Computer Assisted Sperm Analysis system, CASA, enables determination of numerous parameters characterizing sperm motion activity. This system allows for the examination of the effect of various environmental factors on spermatozoa motility parameters. The aim of this work was to compare time-dependent motility changes of sperm obtained from the dace, Leuciscus leuciscus (L.), by abdominal massage (sperm from spermatic ducts) and directly from gonads (testicular sperm). The analysis concerned such sperm motility parameters as: percentage of motile sperm (MOT, %), total sperm velocity (VAP, μm s–1), straight line velocity (VSL, μm s–1), curvilinear velocity (VCL, μm s–1), linearity (LIN: VSL/VCL · 100%), straightness (STR: VSL/VAP · 100%), amplitude of the lateral head displacement (ALH, μm) and beat cross frequency (BCF, Hz). During 105 seconds of motility, no significant differences were found in the values of MOT between sperm originating from the spermatic ducts and from the tes...
Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through ... more Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in samples of stripped and testicular sperm obtained from XY and YY males, and testicular sperm of XX males. The motile spermatozoa, as measured by both subjective method and CASA, showed no differences between testicular sperm of XX males and stripped sperm of XY and YY males whereas testicular sperm of XY and YY males had significantly lower sperm motility. Result of protein densitometry showed similarities in protein profile between seminal plasma of XY and YY males and testicular fluids of XX males. Testis of XX males showed specific histological structures of cysts consists hypertrophied Se...
Journal of the World Aquaculture Society, 2013
ABSTRACT The common carp, Cyprinus carpio L. sperm motility parameters were analyzed by using com... more ABSTRACT The common carp, Cyprinus carpio L. sperm motility parameters were analyzed by using computer-assisted sperm analysis system. The percentage of motile sperm (MOT, %), progressively motile sperm (PRG, %), curvilinear velocity (VCL, μm/sec), average path velocity (VAP, μm/sec), the wobbling index (WOB, %), movement linearity (LIN, %), beat cross frequency (BCF, Hz), and amplitude of lateral head displacement (ALH, μm) were determined. Five activation solutions (As) were used to activate sperm movement. As 1 solution: 68 mM NaCl, 50 mM urea, 0.5% bovine serum albumin (BSA), pH: 7.7, 181 mOsm/kg; As 2 buffer: 100 mM NaCl, 10 mM Tris, 0.5% BSA, pH: 9.0, 199 mOsm/kg; As 3 solution: 86 mM NaCl, 0.5% BSA, pH: 7.4, 167 mOsm/kg; As 4 buffer: 5 mM KCl, 45 mM NaCl, 30 mM Tris, 0.5%, pH: 8.0, 160 mOsm/kg; and As 5 solution: distilled water with the addition of 0.5% BSA, pH: 7.3, <3 mOsm/kg. Among five tested solutions, a buffer with a pH of 9.0 and osmolality of approximately 200 mOsm/kg (As 2) was the most suitable. After its activation, a significant increase in MOT and ALH values was observed, which can be of importance to the effectiveness of egg fertilization.
1 Organisme de formation enregistré sous le numéro 41570143657 auprès du Préfet de la Région Lorr... more 1 Organisme de formation enregistré sous le numéro 41570143657 auprès du Préfet de la Région Lorraine Conférence ELEVAGE DE LA LOTTE (Lota lota) : Recherche et perspectives Jeudi 1 juillet 2004 au Muséum-aquarium de Nancy 34, rue Ste Catherine 54 000 NANCY Tel : 03 83 32 99 97, Fax :03 83 32 30 16 En collaboration avec le Laboratoire de Sciences Animales de L'Université Henri Poincaré Nancy I. Organisé par : Elevage de la lotte (Lota lota)/FLAC/Nancy 2004 _______________________________________________________________________________ Filière Lorraine d'Aquaculture Continentale -67, rue principale -57 260 Lindre-Basse Tel : 03 87 86 07 23 Fax : 03 87 86 07 85
Reproductive Biology, Feb 1, 2006
Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in t... more Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in the environment have adverse effects on the reproductive function in fish. Three studies were conducted to assess toxicity of these chemicals towards reproduction of freshwater fish. It was shown that heavy metals added to the diets accumulate in brain tissue of carp, and this accumulation results in inhibition of the secretion of noradrenaline and stimulation of the secretion of dopamine in the hypothalamus. These processes results in a disturbance of hormonal equilibrium of the hypothalamo-pituitary system, which can unfavorably influence the efficiency of artificial spawning in fish. Quality of salmonid and sturgeon sperm was impaired after in vitro exposure to heavy metals. The degree of this toxic effect was species-specific.
Polish Journal of Natural Science
Acid phosphatase (AcP) is a commonly observed enzyme in animal semen. In this study, AcP in rainb... more Acid phosphatase (AcP) is a commonly observed enzyme in animal semen. In this study, AcP in rainbow trout (Oncorhynchus mykiss) spermatozoa was partly purified and characterized. Extraction in 0.85% NaCl with 0.1% Triton X-100 enabled obtaining 95% of total AcP activity observed in sperm supernatant. Kinetic characteristics were described for the enzyme from sperm extract and for the partly purified
The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthe... more The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) was frozen without cryoprotector (at -79C) or cryopreserved with methanol as the cryoprotector. The activity of arylsulfatase (AS), acid phosphatase (AcP), -N-acetylglucosaminidase (NAGase), and protein concentration was determined. The protein concentration and enzymatic activities in supernatant obtained after cryopreservation were higher than in milt plasma, but
Journal of Applied Ichthyology, 2015
ABSTRACT Acid phosphatase and arylsulphatase are present in the semen of many animal species, inc... more ABSTRACT Acid phosphatase and arylsulphatase are present in the semen of many animal species, including fish. The presence of these two enzymes has also been confirmed in the semen of Siberian sturgeon (Acipenser baerii). The aim of this study was therefore to determine the potential role of acid phosphatase and arylosulphatase in the fertilization process. Tested were the additions of ammonium molybdate (acid phosphatase inhibitor) and AgNO3 (arylsulphatase inhibitor) regarding their effects on the sperm motility spermatozoa rate and the fertilization rate (in two inhibiting variants: enzymes present in spermatozoa, sperm treated; enzymes present in spermatozoa and eggs, sperm + eggs treated). There was no significant deterioration in motility parameters with either of the applied inhibitors. Ammonium molybdate caused a significant decrease in the fertilization rate (sperm + treated eggs from 86.1 to 9.5%); AgNO3 added only to spermatozoa resulted in a complete inhibition of fertilization. Results indicate that the acid phosphatase activity in eggs and arylsuphatase activity in sperm are necessary for the fertilization process in the Siberian sturgeon.
Journal of Applied Ichthyology, 2015
ABSTRACT Compared were the volume and quality of carp Cyprinus carpio L. obtained from semen of m... more ABSTRACT Compared were the volume and quality of carp Cyprinus carpio L. obtained from semen of males not subjected to hormonal stimulation (N = 10) but injected with 0.9% NaCl in the amount of 1 ml kg -1 b.w., as well as those stimulated using Ovopel (N = 10) in the amount of 1 granule kg -1 b.w. Semen was collected from the males 24 h after injection of hormonal preparation, to determine the pH, total volume (TVS) and volume recalculated per kg of body mass, concentration and total sperm production (TSP). Differences in sperm motility parameters between fish groups were determined by a computer-assisted sperm analysis (CASA) system. Sperm motility was activated using 10 mM Tris buffer, containing 100 mM NaCl (pH 9.0, osmolality 200 mOsm kg -1). The pH, osmolality, total protein concentration and activity of acidic phosphatase (AcP), lactate dehydrogenase (LDH), ß-N-acetylglucosaminidase and arylsulfatase (AS) were determined in the seminal plasma of both test groups. The stimulation of male carp using Ovopel did not significantly affect CASA parameters, but did lead to an over ten-fold increase in the values of TVS and VOS and nearly six times higher values of TSP as compared to the Control group. A significant increase in pH following stimulation with Ovopel indicates that cyclic AMP increased, thanks to which sperm acquire a capacity to move during their passage through the sperm ducts. An increase in the values of CASA parameters is a likely reflection of these changes.
Aquaculture Research, 2015
Aquaculture, 2015
Collection of fish milt by stripping risks contamination with urine, which may negatively influen... more Collection of fish milt by stripping risks contamination with urine, which may negatively influence milt characteristics and quality. Urine contamination of milt may be avoided by using a catheter for sperm collection. The objective of this study was to compare basic pikeperch milt parameters, i.e., sperm concentration (SC), pH, osmolality of seminal plasma (OSM), and motility parameters of spermatozoa analyzed with a CASA system (Computer Assisted Sperm Analysis), collected either with a syringe (as the standard method of sperm collection) or with a catheter. Every parameter of pikeperch milt collected with a catheter was significantly higher (P b 0.05; SC = 12.31 ± 3.81 × 10 9 ml −1 , pH = 9.04 ± 0.07, OSM = 311 ± 16 mOsm kg −1 ) than that collected with a syringe (SC = 8.05 ± 3.04 × 10 9 ml −1 , pH = 8.93 ± 0.09, OSM = 228 ± 51 mOsm kg −1 ), which clearly suggests urine contamination of milt collected with the standard procedure. Moreover, milt collected with catheter was characterized by higher spermatozoa motility parameters, e.g., motility rate of sperm collected with a catheter was 73% whereas the motility rate of sperm collected with a syringe did not exceed 35%. The results obtained in this work indicate that the milt collected with the standard procedure is likely to be exposed to contamination with urine, which may cause a significant reduction of quality parameters. Collection of sperm with a catheter was proven to effectively reduce the contamination of sperm with urine. Therefore, it is recommended to collect pikeperch sperm using a catheter.
Journal of Applied Ichthyology, 2015
ABSTRACT The aim of the study was to investigate the effect of osmolality of the activating solut... more ABSTRACT The aim of the study was to investigate the effect of osmolality of the activating solution (AS) on in vitro fertilization of the eggs of common carp, Cyprinus carpio L. Four different AS (NaCl solutions) were used with different osmolalities: AS-0 (distilled water), AS-100 (100 mOsm kg -1), AS-200 (200 mOsm kg -1) and AS-300 (300 mOsm kg -1). Fertilization was performed in Petri dishes using freshly collected sperm. Insemination (sperm addition to activated eggs) was preceded by exposure of egg samples to each AS for 0 (control group), 15, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 s. Embryonic survival was determined 24 h after incubation at 20°C. Additionally, motility parameters (motility rate [MOT, %] and curvilinear velocity [VCL, lm s -1]) of the spermatozoa activated with the tested AS were measured using a CASA (computer-assisted sperm analysis) system. Motility duration (DUR, s) in each AS was also determined. The results revealed a significant effect of osmolality of the AS on the fertilizing capacity of common carp eggs. There were no viable embryos in the group AS-300. In the group AS-0, the eggs lost their ability for fertilization after 90 s; in the remaining groups, eggs could be fertilized for up to 150 s (group AS-100) or 360 s (group AS-200). Fertilization failure in the group AS-300 was caused by the lack of spermatozoa activation in this solution. The present study findings for in vitro common carp fertilization indicate that special attention should be given to the AS osmolality, where 100 mOsm kg -1 was found to be the most suitable.
Polish Journal of Natural Science, 2007
... JONÓW RTĘCI I KADMU NA AKTYWNOŚĆ WYBRANYCH ENZYMÓW W NASIENIU JESIOTRA SYBERYJSKIEGO (ACIPENS... more ... JONÓW RTĘCI I KADMU NA AKTYWNOŚĆ WYBRANYCH ENZYMÓW W NASIENIU JESIOTRA SYBERYJSKIEGO (ACIPENSER BAERI BRANDT 1869) Beata Sarosiek1, Grzegorz Zdanio2,Radosław Kajetan Kowalski1, Jan Glogowski1 1 Instytut Rozrodu Zwierząt i Badań ...
Reproductive biology, 2006
Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in t... more Many industrial and agricultural chemicals (including heavy metals and alkylphenols) present in the environment have adverse effects on the reproductive function in fish. Three studies were conducted to assess toxicity of these chemicals towards reproduction of freshwater fish. It was shown that heavy metals added to the diets accumulate in brain tissue of carp, and this accumulation results in inhibition of the secretion of noradrenaline and stimulation of the secretion of dopamine in the hypothalamus. These processes results in a disturbance of hormonal equilibrium of the hypothalamo-pituitary system, which can unfavorably influence the efficiency of artificial spawning in fish. Quality of salmonid and sturgeon sperm was impaired after in vitro exposure to heavy metals. The degree of this toxic effect was species-specific. It was demonstrated that sperm motility parameters appeared to be good indicators of adverse effects of heavy metals fish sperm. The protection role of seminal ...
Archives of Polish Fisheries, 2008
The aim of the study was to determine the basic quality parameters and selected biochemical marke... more The aim of the study was to determine the basic quality parameters and selected biochemical markers of asp, Aspius aspius (L.), semen after spermiation was stimulated with Ovaprim and Ovopel. Sperm motility and concentration, osmotic pressure, total protein content, and activities of acid phosphatase (AcP), lactate dehydrogenase (LDH), and b-N-acetylglucosaminidase (b-NAG) were determined. It was revealed that higher sperm motility and concentration and higher seminal plasma protein content were obtained after stimulation with Ovaprim. The osmotic pressure of the seminal plasma estimated for males following the administration of Ovopel was higher than after they had been treated with Ovaprim. It was determined that enzyme activity in the seminal plasma of the fish stimulated with Ovaprim was higher in comparison with results obtained after they had been treated with Ovopel. Significant, positive dependencies were confirmed between the concentration of sperm and the total protein content in the seminal plasma (r 2 = 0.492) and the activity of b-NAG (r 2 = 0.779); among the total protein content in the seminal plasma and the activities of AcP (r 2 = 0.476), LDH (r 2 = 0.564), and b-NAG (r 2 = 0.738); and between the activities of AcP and LDH (r 2 = 0.483) and between the activities of LDH and b-NAG (r 2 = 0.844).