Online Mendelian Inheritance in Man (OMIM) (original) (raw)

* 601480

MYOSIN IF; MYO1F

Alternative titles; symbols

MYOSIN ID, FORMERLY; MYO1D, FORMERLY

HGNC Approved Gene Symbol: MYO1F

Cytogenetic location: 19p13.2 Genomic coordinates (GRCh38) : 19:8,520,778-8,577,442 (from NCBI)

TEXT

Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. For further background information on myosins, see MYO1A (601478).

Cloning and Expression

By PCR amplification of RNA from porcine and human cell lines and human liver, Bement et al. (1994) identified 8 to 11 putative myosins representing 6 distinct myosin classes. Among the identified myosins was a cDNA encoding myosin IF.

By screening a kidney cDNA library with a mouse Myo1f probe, Crozet et al. (1997) obtained a cDNA encoding MYO1F. The deduced 1,099-amino acid protein is 91% identical to the mouse protein. Northern blot analysis revealed wide expression of Myo1f in adult mouse tissues.

Gene Function

Kim et al. (2006) demonstrated that MYO1F is expressed predominantly in the mammalian immune system. Cells from Myo1f-deficient mice exhibited abnormally increased adhesion and reduced motility, resulting from augmented exocytosis of beta-2 integrin (600065)-containing granules. In addition, the cortical actin that colocalizes with Myo1f was reduced in Myo1f-deficient cells. In vivo, Myo1f-deficient mice showed increased susceptibility to infection by Listeria monocytogenes and an impaired neutrophil response. Thus, Kim et al. (2006) concluded that Myo1f directs immune cell motility and innate host defense against infection.

Mapping

Hasson et al. (1996) mapped the mouse Myo1f gene to chromosome 17 by interspecific backcross analysis. The location on chromosome 17 predicted that the human homolog would map to either 6p12 or 19p13.3. By fluorescence in situ hybridization, they demonstrated that the human MYO1F gene, designated MYO1D by the authors, maps to 19p13.3-p13.2. Using PCR and radiation hybrid analysis, Crozet et al. (1997) mapped the MYO1F gene to 19p13.3-p13.2.

REFERENCES

  1. Bement, W. M., Hasson, T., Wirth, J. A., Cheney, R. E., Mooseker, M. S.Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types. Proc. Nat. Acad. Sci. 91: 6549-6553, 1994. Erratum: Proc. Nat. Acad. Sci. 91: 11767, 1994. [PubMed: 8022818] [Full Text: https://doi.org/10.1073/pnas.91.14.6549\]
  2. Crozet, F., Amraoui, A. E., Blanchard, S., Lenoir, M., Ripoll, C., Vago, P., Hamel, C., Fizames, C., Levi-Acobas, F., Depetris, D., Mattei, M.-G., Weil, D., Pujol, R., Petit, C.Cloning of the genes encoding two murine and human cochlear unconventional type I myosins. Genomics 40: 332-341, 1997. [PubMed: 9119401] [Full Text: https://doi.org/10.1006/geno.1996.4526\]
  3. Hasson, T., Skowron, J. F., Gilbert, D. J., Avraham, K. B., Perry, W. L., Bement, W. M., Anderson, B. L., Sherr, E. H., Chen, Z.-Y., Greene, L. A., Ward, D. C., Corey, D. P., Mooseker, M. S., Copeland, N. G., Jenkins, N. A.Mapping of unconventional myosins in mouse and human. Genomics 36: 431-439, 1996. [PubMed: 8884266] [Full Text: https://doi.org/10.1006/geno.1996.0488\]
  4. Kim, S. V., Mehal, W. Z., Dong, X., Heinrich, V., Pypaert, M., Mellman, I., Dembo, M., Mooseker, M. S., Wu, D., Flavell, R. A.Modulation of cell adhesion and motility in the immune system by Myo1f. Science 314: 136-139, 2006. [PubMed: 17023661] [Full Text: https://doi.org/10.1126/science.1131920\]

Contributors:

Ada Hamosh - updated : 10/25/2006
Paul J. Converse - updated : 12/7/2001
Mark H. Paalman - updated : 10/23/1996

Creation Date:

Victor A. McKusick : 10/22/1996

Edit History:

alopez : 11/02/2006
terry : 10/25/2006
mgross : 12/7/2001
carol : 7/20/2001
carol : 7/20/2001
mark : 10/23/1996
mark : 10/22/1996