Online Mendelian Inheritance in Man (OMIM) (original) (raw)

* 607947

POTASSIUM CHANNEL REGULATOR; KCNRG

Alternative titles; symbols

DLTET

HGNC Approved Gene Symbol: KCNRG

Cytogenetic location: 13q14.2 Genomic coordinates (GRCh38) : 13:50,015,444-50,020,922 (from NCBI)

TEXT

Description

KCNRG is a soluble protein with characteristics suggesting it forms heterotetramers with voltage-gated K(+) channels (see 176260) and inhibits their function (Ivanov et al., 2003).

Cloning and Expression

By sequence analysis of a region of chromosome 13 frequently deleted in B-cell and prostate tumors (see 109543), followed by EST database analysis, Ivanov et al. (2003) identified 2 splice variants of KCNRG. The deduced 272- and 229-amino acid proteins differ only in their C-terminal sequences. Both share sequence similarity with the cytoplasmic tetramerization domain of voltage-gated K(+) channels, and both contain a putative BTB/POZ domain. RT-PCR analysis detected both transcripts in lung, lymphocytes, and prostate, with lower levels in kidney, stomach, and brain. Expression was detected in several tumor cell lines, including colon carcinoma, epidermal carcinoma, breast cancer, and osteogenic sarcoma, but not in cell lines derived from metastatic prostate carcinoma, ovarian epithelial carcinoma, or osteosarcoma.

By database and PCR analysis, Corcoran et al. (2004) identified several DLTET transcripts, including a putative bicistronic 7.5-kb transcript containing ORFs for both RFP2 (TRIM13; 605661) and DLTET. In addition to the bicistronic transcript, they identified 4 major monocistronic RFP2 transcripts, 3 major monocistronic DLTET transcripts, and transcripts lacking both ORFs. The DLTET transcripts encode proteins containing 272, 229, or 213 amino acids. RT-PCR detected 4 major transcripts ranging from 1.7 to 7.5 kb in all human tissues examined. Bicistronic and monocistronic Rfp2 and Dltet transcripts were present in mouse. In situ hybridization of mouse embryos revealed coexpression of Rfp2/Dltet with Dleu2 (605766), a putative noncoding RNA, in all tissues examined. The RFP2/DLTET gene overlaps the DLEU2 gene in the antisense orientation in both mice and humans, suggesting that DLEU2 may regulate RFP2/DLTET expression.

Gene Function

By patch-clamp analysis of a cultured prostate cell line, Ivanov et al. (2003) detected reduced K(+) current density following transfection of KCNRG.

Gene Structure

Ivanov et al. (2003) determined that the KCNRG gene contains 3 exons and spans more than 5.4 kb.

Corcoran et al. (2004) proposed that RFP2 and DLTET are encoded by a bicistronic gene that spans 24 kb and contains at least 7 exons, including 2 alternative first exons (1A and 1B). In this structure, exon 4 contains most of the DLTET ORF, with some alternative transcripts also using exon 5 or 6. The RFP2/DLTET gene is located entirely between exons 10 and 12 of the DLEU2 gene in the antisense orientation. All RFP2 and DLTET exons arise from DLEU2 introns except for RFP2 exon 1A, which directly overlaps DLEU2 exon 11.

Mapping

By genomic sequence analysis, Ivanov et al. (2003) mapped the KCNRG gene to chromosome 13q14.3.

Corcoran et al. (2004) mapped the mouse Kcnrg gene to a region of chromosome 14 that shares homology of synteny with human chromosome 13q14.3.

REFERENCES

Corcoran, M. M., Hammarsund, M., Zhu, C., Lerner, M., Kapanadze, B., Wilson, B., Larsson, C., Forsberg, L., Ibbotson, R. E., Einhorn, S., Oscier, D. G., Grander, D., Sangfelt, O.DLEU2 encodes an antisense RNA for the putative bicistronic RFP2/LEU5 gene in humans and mouse. Genes Chromosomes Cancer 40: 285-297, 2004. [PubMed: 15188451] [Full Text: https://doi.org/10.1002/gcc.20046\]
Ivanov, D. V., Tyazhelova, T. V., Lemonnier, L., Kononenko, N., Pestova, A. A., Nikitin, E. A., Prevarskaya, N., Skryma, R., Panchin, Y. V., Yankovsky, N. K., Baranova, A. V.A new human gene KCNRG encoding potassium channel regulating protein is a cancer suppressor gene candidate located in 13q14.3. FEBS Lett. 539: 156-160, 2003. [PubMed: 12650944] [Full Text: https://doi.org/10.1016/s0014-5793(03)00211-4\]

Contributors:

Patricia A. Hartz - updated : 7/30/2007

Creation Date:

Patricia A. Hartz : 7/15/2003

Edit History:

alopez : 09/21/2018
carol : 08/29/2014
mgross : 7/30/2007
mgross : 7/15/2003