Online Mendelian Inheritance in Man (OMIM) (original) (raw)

* 611142

CYTOSKELETON-ASSOCIATED PROTEIN 5; CKAP5

Alternative titles; symbols

COLONIC AND HEPATIC TUMOR OVEREXPRESSED GENE; CHTOG

MINI SPINDLES, DROSOPHILA, HOMOLOG OF; MSPS
KIAA0097

HGNC Approved Gene Symbol: CKAP5

Cytogenetic location: 11p11.2 Genomic coordinates (GRCh38) : 11:46,743,048-46,846,280 (from NCBI)

TEXT

Cloning and Expression

By sequencing clones obtained from a size-fractionated immature myeloid cell line cDNA library, Nagase et al. (1995) cloned CKAP5, which they designated KIAA0097. The deduced protein contains 2,032 amino acids. Northern blot analysis detected highest expression in skeletal muscle and lower expression in all other tissues and cell lines examined, except peripheral blood leukocytes.

Using TACC1 (605301) as bait in a yeast 2-hybrid screen of a mammary epithelial cDNA library, Lauffart et al. (2002) cloned CKAP5, which they called CHTOG. CHTOG contains a microtubule-binding domain in its N-terminal half and a KXGS motif for binding tubulin (see 191110) dimers in its C-terminal half.

Gene Function

Using RNA interference in HeLa cells, Gergely et al. (2003) found that TACC3 (605303) depletion did not alter spindle organization, but partly destabilized microtubules and redistributed CHTOG away from spindle microtubules. In CHTOG-depleted cells, relatively robust spindles formed, but they were highly disorganized. Gergely et al. (2003) concluded that CHTOG plays a major role in organizing spindle poles, whereas its role in stabilizing spindle microtubules is minor and, at least in part, mediated via interaction with TACC3.

Brouhard et al. (2008) showed that the Xenopus CKAP5 ortholog, Xmap215, functioned as a processive microtubule polymerase. Recombinant Xmap215 bound free porcine brain tubulin in a 1:1 complex that interacted with the microtubule lattice and targeted the ends by a diffusion-facilitated mechanism. Xmap215 persisted at the plus end and catalyzed the addition of up to 25 tubulin dimers. Under some circumstances, Xmap215 catalyzed the reverse reaction, microtubule shrinkage.

Mapping

Using human-rodent hybrid cell lines, Nagase et al. (1995) mapped the CKAP5 gene to chromosome 11.

REFERENCES

  1. Brouhard, G. J., Stear, J. H., Noetzel, T. L., Al-Bassam, J., Kinoshita, K., Harrison, S. C., Howard, J., Hyman, A. A.XMAP215 is a processive microtubule polymerase. Cell 132: 79-88, 2008. [PubMed: 18191222] [Full Text: https://doi.org/10.1016/j.cell.2007.11.043\]
  2. Gergely, F., Draviam, V. M., Raff, J. W.The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells. Genes Dev. 17: 336-341, 2003. [PubMed: 12569123] [Full Text: https://doi.org/10.1101/gad.245603\]
  3. Lauffart, B., Howell, S. J., Tasch, J. E., Cowell, J. K., Still, I. H.Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells. Biochem. J. 363: 195-200, 2002. [PubMed: 11903063] [Full Text: https://doi.org/10.1042/0264-6021:3630195\]
  4. Nagase, T, Miyajima, N, Tanaka, A., Sazuka, T., Seki, N., Sato, S., Tabata, S., Ishikawa, K., Kawarabayashi, Y., Kotani, H., Nomura, N.Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 2: 37-43, 1995. [PubMed: 7788527] [Full Text: https://doi.org/10.1093/dnares/2.1.37\]

Contributors:

Patricia A. Hartz - updated : 3/12/2008

Creation Date:

Patricia A. Hartz : 6/27/2007

Edit History:

mgross : 03/13/2008
terry : 3/12/2008
terry : 3/12/2008
mgross : 6/27/2007