Birsen Bilgici | Samsun Ondokuz Mayis University (original) (raw)

Papers by Birsen Bilgici

Research paper thumbnail of Increased levels of lipid and protein oxidation in rat prefrontal cortex after treatment by lithium, valproic acid, and olanzapine

Naunyn-schmiedebergs Archives of Pharmacology, Apr 24, 2023

Research paper thumbnail of Protective Effect of Lycopene against Radiation-Induced Hepatic Toxicity in Rats

Journal of International Medical Research, Aug 1, 2011

The radioprotective effect of lycopene against liver damage was investigated in 80 female Sprague... more The radioprotective effect of lycopene against liver damage was investigated in 80 female Sprague Dawley rats (10 per group). Early-group rats included: controls (group 1), lycopene (group 2), radiotherapy alone (group 3), and lycopene + radiotherapy (group 4). Lycopene (5 mg/kg per day) was administered orally for 7 days; single-fraction 8 Gy abdominopelvic radiotherapy was administered on day 8. Early-group rats were sacrificed on day 10. Late-group rats (groups 5 – 8) underwent treatment with the same regimens but, in groups 6 and 8, lycopene was administered until all rats were sacrificed, 60 days postradiotherapy. Liver malondialdehyde levels increased significantly and glutathione (GSH) levels, GSH-peroxidase (GSH-Px) and superoxide dismutase (SOD) activity decreased significantly in radiotherapy versus control groups. In lycopene + radiotherapy groups, malondialdehyde levels decreased significantly and GSH levels, GSH-Px and SOD activity increased significantly compared with radiotherapy groups. No significant between-group histopathological differences were observed in early groups; in late groups, histopathological changes increased significantly in the radiotherapy group versus control group. A significant decrease in histopathological changes occurred in the lycopene + radiotherapy group compared with the radiotherapy group. Lycopene supplementation significantly reduced radiotherapy-induced oxidative liver injury.

Research paper thumbnail of What is adverse effect of wireless local area network, using 2.45 GHz, on the reproductive system?

International Journal of Radiation Biology, Sep 27, 2018

Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low... more Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low level of electromagnetic fields (EMF) at 2.45 GHz microwave radiation. Methods: Twenty two Wistar rats were divided into two groups. Group 1 was the control group and not exposed to EMF. Group 2 was exposed to low level EMF (average E-field 3.68 ± 0.36 V/m, whole body average SAR, 0.0233 W/kg, in 10 g tissue) at 2.45 GHz for 1 hour/day for 30 consecutive days. At the end of the study, interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-32 (IL-32), C-reactive protein (CRP) were measured in rat serum and IL-6, IL-10, IL-32 were measured in rat testis tissue. Furthermore, testicular tissues were evaluated histopathologically in terms of spermatogenesis and coagulation necrosis. Results: Serum IL-6 and CRP levels were found to be significantly different in the study group compared to the control group (p < .05), but no significant difference was found in serum IL-10, IL-32 levels and testis tissue IL-6, IL-10, IL-32 levels compared to the control group (p > .05). On the other hand, histopathological evaluation of testicular tissue revealed a significant difference in necrosis and spermatogenesis when compared with the control group (p < .05). Conclusions: It may be concluded that low level EMF at 2.45 GHz increases inflammation and testicular damage and negative impact on male reproductive system function.

Research paper thumbnail of Çok işlevli Proteinler: Moonlight Proteinler

Turkish journal of clinics and laboratory, Feb 26, 2020

Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen-bir protein-b... more Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen-bir protein-bir fonksiyon fikrinin yerini almaya başlamıştır. Moonlight proteinler çok fonksiyonlu proteinlerin bir alt sınıfıdır. Moonlight protein kavramı, tek bir polipeptid zincirinin çoklu biyokimyasal fonksiyonları yerine getirmesini tanımlamaktadır. Bugün 300'den fazla moonlight proteini tanımlanmıştır. Bununla birlikte, veriler daha fazla moonlight proteini olabileceğini göstermektedir. Moonlight proteinlerin bilinen örnekleri arasında, reseptörler, enzimler, transkripsiyon faktörleri, adezinler ve hücre iskeleti de dahil olmak üzere çeşitli protein türleri bulunmaktadır. Bir moonlight protein, farklı hücre tiplerinde, farklı hücre içi lokasyonlarda, farklı oligomerik durumlarda bulunarak veya bir ligandın, substratın, kofaktörün ya da ürünün konsantrasyonundaki değişikliklere bağlı olarak ikinci fonksiyonunu aktifleştirebilmektedir. Ancak bu mekanizmalar, özgül değildir ve fonksiyonlar arasındaki geçişlerde bu yollardan birini ya da bunların bir kombinasyonunu kullanabilmektedir. Moonlight proteinlerin, nörodejeneratif hastalıklar ve kanser gibi hastalık fenotipleri ile ilişkili olabileceği öne sürülmektedir. Bunun yanısıra hastalık patogenezlerinde yer alarak tedavi süreçlerine bir takım zorluklar getirmekle birlikte potansiyel bir tedavi hedefi ve tedavi aracı olarak da fırsatlar sunmaktadır. Biz bu derlemede, başlıca insanlardaki mevcut moonlight proteinlerin biyokimyasal, fizyolojik ve patolojik özelliklerini ve hastalıklarla ilişkilerini tartışarak mevcut temel ve biyokimyasal bilgilerimize katkıda bulunmayı amaçladık.

Research paper thumbnail of Dapson Tedavisine Bağlı Hemoliz Görülen Bir Hastada Hemoglobin D Saptanması

Kocaeli Üniversitesi sağlık bilimleri dergisi, Sep 6, 2019

Research paper thumbnail of Antioxidant effect of caffeic acid phenethyl ester in experimentally induced periodontitis

Clinical Oral Investigations, Mar 26, 2021

OBJECTIVES The aim of the present study was to evaluate the antioxidant effect of systemically ad... more OBJECTIVES The aim of the present study was to evaluate the antioxidant effect of systemically administered caffeic acid phenethyl ester (CAPE) in periodontitis. MATERIALS AND METHODS Forty rats were randomly divided into four groups: control, lipopolysaccharide-induced experimental periodontitis (LPS), CAPE 5: LPS+5 μmol/kg/day CAPE, and CAPE 10: LPS+10 μmol/kg/day CAPE. Following lipopolysaccharide-induced experimental periodontitis, CAPE was administered intraperitoneally for 28 days. Gingival and serumal total antioxidant status (TAS) and total oxidant status (TOS) were analyzed by enzyme-linked immunosorbent assay (ELISA). RESULTS Gingival tissue TAS was significantly higher with CAPE application compared with the LPS group and was highest in the CAPE 10 group (p<0.05). Gingival tissue TOS was highest in the LPS group, and both of the CAPE dosages decreased the gingival tissue TOS, with the highest decrease in the CAPE 10 group (p<0.05). The differences were not significant for serumal TAS or TOS levels (p>0.05). CONCLUSIONS The effect of CAPE on increased TAS and decreased TOS levels in inflamed gingival tissue indicates the antioxidant therapeutic potential of CAPE in periodontitis. CLINICAL RELEVANCE Within the limitations of this study, CAPE may be suggested as an effective host modulator agent for reducing oxidative stress in gingival tissue and might be considered as an adjunctive therapy in periodontitis.

Research paper thumbnail of Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats

Research paper thumbnail of Is There any Association Between Low Level of Serum Nesfatin-1 and Fibromyalgia Syndrome?

Archives of Rheumatology, 2020

Objectives: This study aims to investigate the relationship between serum level of nesfatin-1 and... more Objectives: This study aims to investigate the relationship between serum level of nesfatin-1 and fibromyalgia syndrome (FMS) clinical parameters such as pain severity, disease activity, fatigue, emotional state, and sleep quality. Patients and methods: Forty-six female patients with FMS (median age 40 years; range, 18 to 53 years) and 46 healthy female controls (median age 36 years; range, 19 to 52 years) were included in the study. Severity of pain, disease activity, fatigue, sleep quality, and emotional status were evaluated by visual analog scale, Fibromyalgia Impact Questionnaire, Multidimensional Assessment of Fatigue (MAF), Pittsburgh Sleep Quality Index (PSQI), Beck Depression Inventory (BDI), and Beck Anxiety Inventory (BAI), respectively. Serum nesfatin-1 concentrations (pg/mL) were measured by enzyme-linked immunosorbent assay method. Results: There was no significant difference with respect to demographic characteristics between the FMS patients and healthy controls. When clinical parameters were compared, MAF, BDI, BAI, and PSQI scores were significantly higher in FMS patients than controls (p<0.05). Serum nesfatin-1 concentration was significantly lower in patients with FMS (p<0.05). When compared to the FMS patients without anxiety, serum nesfatin-1 concentration was significantly increased in FMS patients with anxiety (p<0.05). Serum nesfatin-1 concentration was positively correlated with BAI scores in patients with FMS (p<0.05). Conclusion: Low nesfatin-1 serum levels may contribute to pathological changes in FMS. In addition, nesfatin-1 may also be involved in the mediation of anxiety-related responses in FMS.

Research paper thumbnail of Neurotrophic factors in bipolar disorders patients with manic episode

TURKISH JOURNAL OF MEDICAL SCIENCES, 2020

Background/aim: Neurotrophins are one of the most important molecule groups affecting cerebral ne... more Background/aim: Neurotrophins are one of the most important molecule groups affecting cerebral neuroplasticity. The amount of evidence about the role of changes in neuroplasticity in the pathophysiology of bipolar disease is growing. Materials and methods: We measured serum levels of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), neurotrophin-3 (NT-3), glial cell-line derived neurotrophic factor (GDNF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), fibroblast growth factor (FGF)-2, neuritin 1 (Nrn 1) in bipolar 1 manic episode patients (n = 45) and healthy control group. Results: When controlled for age, BMI and cortisol, it was found that the serum levels of BDNF, NGF, NT-3, VEGF and FGF-2 of bipolar manic episode patients were not statistically different compared to those of the control group. GDNF level and Nrn 1 levels were significantly lower (P = 0.003 and P = 0.025 respectively) while IGF-1 levels were significantly higher than the control group (P = 0.0001). ROC analysis was performed and the area under the the curve was calculated as 0.737, 0.766 for GDNF, IGF-1 respectively. Conclusion: The changes in the levels of GDNF, IGF-1 and Nrn 1 might be involved in pathopysiology of bipolar disorder, and GDNF, IGF-1 may be considered as state markers in bipolar manic episode.

Research paper thumbnail of Çok işlevli Proteinler: Moonlight Proteinler

Turkish Journal of Clinics and Laboratory, 2020

Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen - bir protein... more Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen - bir protein - bir fonksiyon fikrinin yerini almaya başlamıştır. Moonlight proteinler çok fonksiyonlu proteinlerin bir alt sınıfıdır. Moonlight protein kavramı, tek bir polipeptid zincirinin çoklu biyokimyasal fonksiyonları yerine getirmesini tanımlamaktadır. Bugün 300'den fazla moonlight proteini tanımlanmıştır. Bununla birlikte, veriler daha fazla moonlight proteini olabileceğini göstermektedir. Moonlight proteinlerin bilinen örnekleri arasında, reseptörler, enzimler, transkripsiyon faktörleri, adhezinler ve hücre iskeleti de dahil olmak üzere çeşitli protein türleri bulunmaktadır. Bir moonlight protein, farklı hücre tiplerinde, farklı hücre içi lokasyonlarda, farklı oligomerik durumlarda bulunarak veya bir ligandın, substratın, kofaktörün ya da ürünün konsantrasyonundaki değişikliklere bağlı olarak ikinci fonksiyonunu aktifleştirebilmektedir. Ancak bu mekanizmalar, özgül değildir ve fonksiyon...

Research paper thumbnail of What is adverse effect of wireless local area network, using 2.45 GHz, on the reproductive system?

International Journal of Radiation Biology, 2018

Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low... more Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low level of electromagnetic fields (EMF) at 2.45 GHz microwave radiation. Methods: Twenty two Wistar rats were divided into two groups. Group 1 was the control group and not exposed to EMF. Group 2 was exposed to low level EMF (average E-field 3.68 ± 0.36 V/m, whole body average SAR, 0.0233 W/kg, in 10 g tissue) at 2.45 GHz for 1 hour/day for 30 consecutive days. At the end of the study, interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-32 (IL-32), C-reactive protein (CRP) were measured in rat serum and IL-6, IL-10, IL-32 were measured in rat testis tissue. Furthermore, testicular tissues were evaluated histopathologically in terms of spermatogenesis and coagulation necrosis. Results: Serum IL-6 and CRP levels were found to be significantly different in the study group compared to the control group (p < .05), but no significant difference was found in serum IL-10, IL-32 levels and testis tissue IL-6, IL-10, IL-32 levels compared to the control group (p > .05). On the other hand, histopathological evaluation of testicular tissue revealed a significant difference in necrosis and spermatogenesis when compared with the control group (p < .05). Conclusions: It may be concluded that low level EMF at 2.45 GHz increases inflammation and testicular damage and negative impact on male reproductive system function.

Research paper thumbnail of Effect of bisphosphonate as an adjunct treatment for chronic periodontitis on gingival crevicuar fluid levels of nuclear factor-κB ligand (RANKL) and osteoprotegerin in postmenopausal osteoporosis

Journal of oral science, 2017

Osteoporosis and periodontal disease are linked by an altered receptor activator of nuclear facto... more Osteoporosis and periodontal disease are linked by an altered receptor activator of nuclear factor κB ligand and osteoprotegerin ratio (RANKL/OPG), and medical treatment with bisphosphonate (BP) may help control these molecules. The effect of BP on clinical findings and gingival crevicular fluid (GCF) values of RANKL and OPG using enzyme-linked immunosorbent assays was evaluated in postmenopausal women; 13 patients with both chronic periodontitis and osteoporosis (group A), 12 systemically healthy patients with chronic periodontitis (group B), 12 periodontally healthy patients with osteoporosis (group C), and 10 systemically and periodontally healthy individuals (group D). Recordings were repeated at the end of months 1, 6, and 12 in groups A, B, and C. At the baseline, groups A and B exhibited the lowest OPG values (P < 0.05). After periodontal treatment, OPG values were markedly increased at the end of 6th month in group A and 12th month in group B (P < 0.008). There was no ...

Research paper thumbnail of WITHDRAWN: Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats

Research paper thumbnail of Effects of periodontal and bisphosphonate treatment on the gingival crevicular levels of sclerostin and dickkopf‐1 in postmenopausal osteoporosis with and without periodontitis

Journal of Periodontal Research

Research paper thumbnail of Ribosomal Proteins and Cancer

The Eurasia Proceedings of Health, Environment and Life Sciences, 2022

The translation process consists of translation factors and ribosomes. Ribosomal components inclu... more The translation process consists of translation factors and ribosomes. Ribosomal components include ribosomal proteins (RP) and ribosomal RNA. Many RPs are involved in assembling ribosomal particles and or stabilizing important regions of rRNA. Besides their conventional roles, RPs have been reported to exhibit secondary functions that have not yet been fully characterized in other cellular processes such as DNA repair, apoptosis, drug resistance, proliferation, and growth inhibition. Since cancer cells require a large amount of protein, they need ribosomes that work much more efficiently than normal cells. Several tumor suppressors and oncogenic proteins control the progression of cancer cells by regulating ribosome biogenesis and protein synthesis. Interestingly, free RPs also have diverse roles in tumorigenesis or tumor suppression. The physiological link between RPs and cancers has been extensively reviewed and elucidated on several pathways, including their interaction with the...

Research paper thumbnail of Effects of smoking on the gingival crevicular fluid levels of interleukin‐17A, interleukin‐17E, and oxidative stress following periodontal treatment process

Journal of Periodontal Research, 2021

OBJECTIVE AND BACKGROUND How smoking affects periodontal inflammation and healing still needs to ... more OBJECTIVE AND BACKGROUND How smoking affects periodontal inflammation and healing still needs to be revealed with all its mechanisms. In this study, the gingival crevicular fluid (GCF) levels of: (a) interleukin-17A (IL-17A) and interleukin-17E(IL-17E) with their ratios and (b) oxidative stress by means of total oxidative stress (TOS), total anti-oxidant capacity (TAOC), and their ratios as the oxidative stress index (OSI) were evaluated and compared for smoking and non-smoking periodontitis patients after a periodontitis management process including both the non-surgical and surgical treatments. MATERIALS AND METHODS Fifteen smoker and 15 non-smoker generalized periodontitis patients as 2 distinct groups participated in the study. Conventional clinical and radiographical examinations were utilized for the periodontitis diagnosis. The clinical data and GCF samples were collected at baseline, 4 week after non-surgical periodontal treatment (NSPT), and 4 weeks after surgical periodontal treatment (SPT). IL-17A, IL-17E, TOS, and TAOC were determined by ELISA and Rel Assay. RESULTS Clinical parameters in both smokers and non-smokers improved following periodontal treatment (P < .001) and their clinical data were similar for all the examination times (baseline, NSPT, and SPT) (P > .05). Following the treatment phases, the IL-17A concentration decreased and the IL-17E concentration increased in both the smokers and non-smokers (P < .01). The total amount of IL-17A decreased while the total amount of IL-17E increased in smokers throughout NSPT and SPT (P < .01). Such an alteration was seen only at SPT compared to NSPT and baseline in non-smokers (P < .01). The concentration and total amount of IL-17A were higher at baseline, and the concentration and total amount of IL-17E were lower at all examination time points in non-smokers as compared to smokers (P < .01). The 17A/E ratio decreased in both groups following the treatment phases and was higher in smokers at all the examination times (P < .01). TOS were higher and TAOC were lower in smokers versus non-smokers at all the time points, but the differences were significant only for TOS levels (P < .01). Throughout the treatment phases, the concentration and total amount of TOS decreased in smokers(P < .01) and only the total amount of TOS decreased in non-smokers (P < .01). The concentration and total amounts of TAOC increased throughout the treatments in both smokers and non-smokers without significant changes (P > .05). The baseline OSI was higher in smokers, and it decreased only in smokers following the treatment phases (P < .01). CONCLUSIONS Smoking and periodontal inflammation were found to alter IL-17A, IL-17E, and oxidant/anti-oxidant statuses in periodontitis patients. The intra-group assessments in smokers demonstrated more apparent alterations in the oxidant/anti-oxidant statuses and IL-17A and IL-17E levels after periodontitis management.

Research paper thumbnail of Changes in IGF-I, IGFBP-3 and ghrelin levels after adenotonsillectomy in children with sleep disordered breathing

International Journal of Pediatric Otorhinolaryngology, 2009

The aim of this study was to determine the changes in insulin-like growth factor-I (IGF-I), insul... more The aim of this study was to determine the changes in insulin-like growth factor-I (IGF-I), insulin-like growth factor binding protein-3 (IGFBP-3) and ghrelin levels following adenotonsillectomy (T&A) in children with sleep disordered breathing (SDB). Methods: Forty children (mean age 4.85 AE 2.15 years) clinically diagnosed with adenotonsillar hypertrophy (ATH) related SDB were enrolled. All children underwent T&A. Serum levels of IGF-I, IGFBP-3 and ghrelin were measured before and 6 months after T&A. Results: Serum levels of IGF-I and IGFBP-3 were significantly higher after T&A (p < 0.001). Serum ghrelin levels showed a significant decrease after T&A (p < 0.001). Conclusion: Children with ATH related SDB who underwent T&A showed significant increases in IGF-I and IGFBP-3 levels indicating an increase in diurnal growth hormone secretion as well as a significant decrese in ghrelin levels indicating an increased oral food intake in the postoperative period.

Research paper thumbnail of Dikkat Eksikliği ve Hiperaktivite Bozukluğu olan Çocuklarda Semptomlar ile Serum Demir ve Ferritin Seviyeleri Arasındaki İlişki

Nöro psikiyatri Arşivi, 2011

Research paper thumbnail of Article Influence of -tocopherol and -lipoic

acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of rats

Research paper thumbnail of Association Between Symptom Profiles and Iron and Ferritine Serum Levels in Children with Attention Deficit Hyperactivity Disorder

Ahmet fiENSES, Saliha BAYKAL, Ömer BÖKE Ondokuz May›s Üniversitesi T›p Fakültesi, Çocuk ve Ergen ... more Ahmet fiENSES, Saliha BAYKAL, Ömer BÖKE Ondokuz May›s Üniversitesi T›p Fakültesi, Çocuk ve Ergen Ruh Sa¤l›¤› Bilim Dal›, Samsun, Türkiye *Ondokuz May›s Üniversitesi T›p Fakültesi, Biyokimya Bilim Dal›, Samsun, Türkiye **Ondokuz May›s Üniversitesi T›p Fakültesi, Çocuk Sa¤l›¤› ve Hastal›klar› Bilim Dal›, Samsun, Türkiye Dikkat Eksikli¤i ve Hiperaktivite Bozuklu¤u olan Çocuklarda Semptomlar ile Serum Demir ve Ferritin Seviyeleri Aras›ndaki ‹liflki

Research paper thumbnail of Increased levels of lipid and protein oxidation in rat prefrontal cortex after treatment by lithium, valproic acid, and olanzapine

Naunyn-schmiedebergs Archives of Pharmacology, Apr 24, 2023

Research paper thumbnail of Protective Effect of Lycopene against Radiation-Induced Hepatic Toxicity in Rats

Journal of International Medical Research, Aug 1, 2011

The radioprotective effect of lycopene against liver damage was investigated in 80 female Sprague... more The radioprotective effect of lycopene against liver damage was investigated in 80 female Sprague Dawley rats (10 per group). Early-group rats included: controls (group 1), lycopene (group 2), radiotherapy alone (group 3), and lycopene + radiotherapy (group 4). Lycopene (5 mg/kg per day) was administered orally for 7 days; single-fraction 8 Gy abdominopelvic radiotherapy was administered on day 8. Early-group rats were sacrificed on day 10. Late-group rats (groups 5 – 8) underwent treatment with the same regimens but, in groups 6 and 8, lycopene was administered until all rats were sacrificed, 60 days postradiotherapy. Liver malondialdehyde levels increased significantly and glutathione (GSH) levels, GSH-peroxidase (GSH-Px) and superoxide dismutase (SOD) activity decreased significantly in radiotherapy versus control groups. In lycopene + radiotherapy groups, malondialdehyde levels decreased significantly and GSH levels, GSH-Px and SOD activity increased significantly compared with radiotherapy groups. No significant between-group histopathological differences were observed in early groups; in late groups, histopathological changes increased significantly in the radiotherapy group versus control group. A significant decrease in histopathological changes occurred in the lycopene + radiotherapy group compared with the radiotherapy group. Lycopene supplementation significantly reduced radiotherapy-induced oxidative liver injury.

Research paper thumbnail of What is adverse effect of wireless local area network, using 2.45 GHz, on the reproductive system?

International Journal of Radiation Biology, Sep 27, 2018

Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low... more Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low level of electromagnetic fields (EMF) at 2.45 GHz microwave radiation. Methods: Twenty two Wistar rats were divided into two groups. Group 1 was the control group and not exposed to EMF. Group 2 was exposed to low level EMF (average E-field 3.68 ± 0.36 V/m, whole body average SAR, 0.0233 W/kg, in 10 g tissue) at 2.45 GHz for 1 hour/day for 30 consecutive days. At the end of the study, interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-32 (IL-32), C-reactive protein (CRP) were measured in rat serum and IL-6, IL-10, IL-32 were measured in rat testis tissue. Furthermore, testicular tissues were evaluated histopathologically in terms of spermatogenesis and coagulation necrosis. Results: Serum IL-6 and CRP levels were found to be significantly different in the study group compared to the control group (p < .05), but no significant difference was found in serum IL-10, IL-32 levels and testis tissue IL-6, IL-10, IL-32 levels compared to the control group (p > .05). On the other hand, histopathological evaluation of testicular tissue revealed a significant difference in necrosis and spermatogenesis when compared with the control group (p < .05). Conclusions: It may be concluded that low level EMF at 2.45 GHz increases inflammation and testicular damage and negative impact on male reproductive system function.

Research paper thumbnail of Çok işlevli Proteinler: Moonlight Proteinler

Turkish journal of clinics and laboratory, Feb 26, 2020

Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen-bir protein-b... more Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen-bir protein-bir fonksiyon fikrinin yerini almaya başlamıştır. Moonlight proteinler çok fonksiyonlu proteinlerin bir alt sınıfıdır. Moonlight protein kavramı, tek bir polipeptid zincirinin çoklu biyokimyasal fonksiyonları yerine getirmesini tanımlamaktadır. Bugün 300'den fazla moonlight proteini tanımlanmıştır. Bununla birlikte, veriler daha fazla moonlight proteini olabileceğini göstermektedir. Moonlight proteinlerin bilinen örnekleri arasında, reseptörler, enzimler, transkripsiyon faktörleri, adezinler ve hücre iskeleti de dahil olmak üzere çeşitli protein türleri bulunmaktadır. Bir moonlight protein, farklı hücre tiplerinde, farklı hücre içi lokasyonlarda, farklı oligomerik durumlarda bulunarak veya bir ligandın, substratın, kofaktörün ya da ürünün konsantrasyonundaki değişikliklere bağlı olarak ikinci fonksiyonunu aktifleştirebilmektedir. Ancak bu mekanizmalar, özgül değildir ve fonksiyonlar arasındaki geçişlerde bu yollardan birini ya da bunların bir kombinasyonunu kullanabilmektedir. Moonlight proteinlerin, nörodejeneratif hastalıklar ve kanser gibi hastalık fenotipleri ile ilişkili olabileceği öne sürülmektedir. Bunun yanısıra hastalık patogenezlerinde yer alarak tedavi süreçlerine bir takım zorluklar getirmekle birlikte potansiyel bir tedavi hedefi ve tedavi aracı olarak da fırsatlar sunmaktadır. Biz bu derlemede, başlıca insanlardaki mevcut moonlight proteinlerin biyokimyasal, fizyolojik ve patolojik özelliklerini ve hastalıklarla ilişkilerini tartışarak mevcut temel ve biyokimyasal bilgilerimize katkıda bulunmayı amaçladık.

Research paper thumbnail of Dapson Tedavisine Bağlı Hemoliz Görülen Bir Hastada Hemoglobin D Saptanması

Kocaeli Üniversitesi sağlık bilimleri dergisi, Sep 6, 2019

Research paper thumbnail of Antioxidant effect of caffeic acid phenethyl ester in experimentally induced periodontitis

Clinical Oral Investigations, Mar 26, 2021

OBJECTIVES The aim of the present study was to evaluate the antioxidant effect of systemically ad... more OBJECTIVES The aim of the present study was to evaluate the antioxidant effect of systemically administered caffeic acid phenethyl ester (CAPE) in periodontitis. MATERIALS AND METHODS Forty rats were randomly divided into four groups: control, lipopolysaccharide-induced experimental periodontitis (LPS), CAPE 5: LPS+5 μmol/kg/day CAPE, and CAPE 10: LPS+10 μmol/kg/day CAPE. Following lipopolysaccharide-induced experimental periodontitis, CAPE was administered intraperitoneally for 28 days. Gingival and serumal total antioxidant status (TAS) and total oxidant status (TOS) were analyzed by enzyme-linked immunosorbent assay (ELISA). RESULTS Gingival tissue TAS was significantly higher with CAPE application compared with the LPS group and was highest in the CAPE 10 group (p<0.05). Gingival tissue TOS was highest in the LPS group, and both of the CAPE dosages decreased the gingival tissue TOS, with the highest decrease in the CAPE 10 group (p<0.05). The differences were not significant for serumal TAS or TOS levels (p>0.05). CONCLUSIONS The effect of CAPE on increased TAS and decreased TOS levels in inflamed gingival tissue indicates the antioxidant therapeutic potential of CAPE in periodontitis. CLINICAL RELEVANCE Within the limitations of this study, CAPE may be suggested as an effective host modulator agent for reducing oxidative stress in gingival tissue and might be considered as an adjunctive therapy in periodontitis.

Research paper thumbnail of Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats

Research paper thumbnail of Is There any Association Between Low Level of Serum Nesfatin-1 and Fibromyalgia Syndrome?

Archives of Rheumatology, 2020

Objectives: This study aims to investigate the relationship between serum level of nesfatin-1 and... more Objectives: This study aims to investigate the relationship between serum level of nesfatin-1 and fibromyalgia syndrome (FMS) clinical parameters such as pain severity, disease activity, fatigue, emotional state, and sleep quality. Patients and methods: Forty-six female patients with FMS (median age 40 years; range, 18 to 53 years) and 46 healthy female controls (median age 36 years; range, 19 to 52 years) were included in the study. Severity of pain, disease activity, fatigue, sleep quality, and emotional status were evaluated by visual analog scale, Fibromyalgia Impact Questionnaire, Multidimensional Assessment of Fatigue (MAF), Pittsburgh Sleep Quality Index (PSQI), Beck Depression Inventory (BDI), and Beck Anxiety Inventory (BAI), respectively. Serum nesfatin-1 concentrations (pg/mL) were measured by enzyme-linked immunosorbent assay method. Results: There was no significant difference with respect to demographic characteristics between the FMS patients and healthy controls. When clinical parameters were compared, MAF, BDI, BAI, and PSQI scores were significantly higher in FMS patients than controls (p<0.05). Serum nesfatin-1 concentration was significantly lower in patients with FMS (p<0.05). When compared to the FMS patients without anxiety, serum nesfatin-1 concentration was significantly increased in FMS patients with anxiety (p<0.05). Serum nesfatin-1 concentration was positively correlated with BAI scores in patients with FMS (p<0.05). Conclusion: Low nesfatin-1 serum levels may contribute to pathological changes in FMS. In addition, nesfatin-1 may also be involved in the mediation of anxiety-related responses in FMS.

Research paper thumbnail of Neurotrophic factors in bipolar disorders patients with manic episode

TURKISH JOURNAL OF MEDICAL SCIENCES, 2020

Background/aim: Neurotrophins are one of the most important molecule groups affecting cerebral ne... more Background/aim: Neurotrophins are one of the most important molecule groups affecting cerebral neuroplasticity. The amount of evidence about the role of changes in neuroplasticity in the pathophysiology of bipolar disease is growing. Materials and methods: We measured serum levels of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), neurotrophin-3 (NT-3), glial cell-line derived neurotrophic factor (GDNF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), fibroblast growth factor (FGF)-2, neuritin 1 (Nrn 1) in bipolar 1 manic episode patients (n = 45) and healthy control group. Results: When controlled for age, BMI and cortisol, it was found that the serum levels of BDNF, NGF, NT-3, VEGF and FGF-2 of bipolar manic episode patients were not statistically different compared to those of the control group. GDNF level and Nrn 1 levels were significantly lower (P = 0.003 and P = 0.025 respectively) while IGF-1 levels were significantly higher than the control group (P = 0.0001). ROC analysis was performed and the area under the the curve was calculated as 0.737, 0.766 for GDNF, IGF-1 respectively. Conclusion: The changes in the levels of GDNF, IGF-1 and Nrn 1 might be involved in pathopysiology of bipolar disorder, and GDNF, IGF-1 may be considered as state markers in bipolar manic episode.

Research paper thumbnail of Çok işlevli Proteinler: Moonlight Proteinler

Turkish Journal of Clinics and Laboratory, 2020

Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen - bir protein... more Son yıllarda birçok proteinin birden fazla fonksiyona sahip olduğu bilgisi, bir gen - bir protein - bir fonksiyon fikrinin yerini almaya başlamıştır. Moonlight proteinler çok fonksiyonlu proteinlerin bir alt sınıfıdır. Moonlight protein kavramı, tek bir polipeptid zincirinin çoklu biyokimyasal fonksiyonları yerine getirmesini tanımlamaktadır. Bugün 300'den fazla moonlight proteini tanımlanmıştır. Bununla birlikte, veriler daha fazla moonlight proteini olabileceğini göstermektedir. Moonlight proteinlerin bilinen örnekleri arasında, reseptörler, enzimler, transkripsiyon faktörleri, adhezinler ve hücre iskeleti de dahil olmak üzere çeşitli protein türleri bulunmaktadır. Bir moonlight protein, farklı hücre tiplerinde, farklı hücre içi lokasyonlarda, farklı oligomerik durumlarda bulunarak veya bir ligandın, substratın, kofaktörün ya da ürünün konsantrasyonundaki değişikliklere bağlı olarak ikinci fonksiyonunu aktifleştirebilmektedir. Ancak bu mekanizmalar, özgül değildir ve fonksiyon...

Research paper thumbnail of What is adverse effect of wireless local area network, using 2.45 GHz, on the reproductive system?

International Journal of Radiation Biology, 2018

Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low... more Abstract Aim: To investigate the inflammatory effect and testicular damage on rats exposed to low level of electromagnetic fields (EMF) at 2.45 GHz microwave radiation. Methods: Twenty two Wistar rats were divided into two groups. Group 1 was the control group and not exposed to EMF. Group 2 was exposed to low level EMF (average E-field 3.68 ± 0.36 V/m, whole body average SAR, 0.0233 W/kg, in 10 g tissue) at 2.45 GHz for 1 hour/day for 30 consecutive days. At the end of the study, interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-32 (IL-32), C-reactive protein (CRP) were measured in rat serum and IL-6, IL-10, IL-32 were measured in rat testis tissue. Furthermore, testicular tissues were evaluated histopathologically in terms of spermatogenesis and coagulation necrosis. Results: Serum IL-6 and CRP levels were found to be significantly different in the study group compared to the control group (p < .05), but no significant difference was found in serum IL-10, IL-32 levels and testis tissue IL-6, IL-10, IL-32 levels compared to the control group (p > .05). On the other hand, histopathological evaluation of testicular tissue revealed a significant difference in necrosis and spermatogenesis when compared with the control group (p < .05). Conclusions: It may be concluded that low level EMF at 2.45 GHz increases inflammation and testicular damage and negative impact on male reproductive system function.

Research paper thumbnail of Effect of bisphosphonate as an adjunct treatment for chronic periodontitis on gingival crevicuar fluid levels of nuclear factor-κB ligand (RANKL) and osteoprotegerin in postmenopausal osteoporosis

Journal of oral science, 2017

Osteoporosis and periodontal disease are linked by an altered receptor activator of nuclear facto... more Osteoporosis and periodontal disease are linked by an altered receptor activator of nuclear factor κB ligand and osteoprotegerin ratio (RANKL/OPG), and medical treatment with bisphosphonate (BP) may help control these molecules. The effect of BP on clinical findings and gingival crevicular fluid (GCF) values of RANKL and OPG using enzyme-linked immunosorbent assays was evaluated in postmenopausal women; 13 patients with both chronic periodontitis and osteoporosis (group A), 12 systemically healthy patients with chronic periodontitis (group B), 12 periodontally healthy patients with osteoporosis (group C), and 10 systemically and periodontally healthy individuals (group D). Recordings were repeated at the end of months 1, 6, and 12 in groups A, B, and C. At the baseline, groups A and B exhibited the lowest OPG values (P < 0.05). After periodontal treatment, OPG values were markedly increased at the end of 6th month in group A and 12th month in group B (P < 0.008). There was no ...

Research paper thumbnail of WITHDRAWN: Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats

Research paper thumbnail of Effects of periodontal and bisphosphonate treatment on the gingival crevicular levels of sclerostin and dickkopf‐1 in postmenopausal osteoporosis with and without periodontitis

Journal of Periodontal Research

Research paper thumbnail of Ribosomal Proteins and Cancer

The Eurasia Proceedings of Health, Environment and Life Sciences, 2022

The translation process consists of translation factors and ribosomes. Ribosomal components inclu... more The translation process consists of translation factors and ribosomes. Ribosomal components include ribosomal proteins (RP) and ribosomal RNA. Many RPs are involved in assembling ribosomal particles and or stabilizing important regions of rRNA. Besides their conventional roles, RPs have been reported to exhibit secondary functions that have not yet been fully characterized in other cellular processes such as DNA repair, apoptosis, drug resistance, proliferation, and growth inhibition. Since cancer cells require a large amount of protein, they need ribosomes that work much more efficiently than normal cells. Several tumor suppressors and oncogenic proteins control the progression of cancer cells by regulating ribosome biogenesis and protein synthesis. Interestingly, free RPs also have diverse roles in tumorigenesis or tumor suppression. The physiological link between RPs and cancers has been extensively reviewed and elucidated on several pathways, including their interaction with the...

Research paper thumbnail of Effects of smoking on the gingival crevicular fluid levels of interleukin‐17A, interleukin‐17E, and oxidative stress following periodontal treatment process

Journal of Periodontal Research, 2021

OBJECTIVE AND BACKGROUND How smoking affects periodontal inflammation and healing still needs to ... more OBJECTIVE AND BACKGROUND How smoking affects periodontal inflammation and healing still needs to be revealed with all its mechanisms. In this study, the gingival crevicular fluid (GCF) levels of: (a) interleukin-17A (IL-17A) and interleukin-17E(IL-17E) with their ratios and (b) oxidative stress by means of total oxidative stress (TOS), total anti-oxidant capacity (TAOC), and their ratios as the oxidative stress index (OSI) were evaluated and compared for smoking and non-smoking periodontitis patients after a periodontitis management process including both the non-surgical and surgical treatments. MATERIALS AND METHODS Fifteen smoker and 15 non-smoker generalized periodontitis patients as 2 distinct groups participated in the study. Conventional clinical and radiographical examinations were utilized for the periodontitis diagnosis. The clinical data and GCF samples were collected at baseline, 4 week after non-surgical periodontal treatment (NSPT), and 4 weeks after surgical periodontal treatment (SPT). IL-17A, IL-17E, TOS, and TAOC were determined by ELISA and Rel Assay. RESULTS Clinical parameters in both smokers and non-smokers improved following periodontal treatment (P < .001) and their clinical data were similar for all the examination times (baseline, NSPT, and SPT) (P > .05). Following the treatment phases, the IL-17A concentration decreased and the IL-17E concentration increased in both the smokers and non-smokers (P < .01). The total amount of IL-17A decreased while the total amount of IL-17E increased in smokers throughout NSPT and SPT (P < .01). Such an alteration was seen only at SPT compared to NSPT and baseline in non-smokers (P < .01). The concentration and total amount of IL-17A were higher at baseline, and the concentration and total amount of IL-17E were lower at all examination time points in non-smokers as compared to smokers (P < .01). The 17A/E ratio decreased in both groups following the treatment phases and was higher in smokers at all the examination times (P < .01). TOS were higher and TAOC were lower in smokers versus non-smokers at all the time points, but the differences were significant only for TOS levels (P < .01). Throughout the treatment phases, the concentration and total amount of TOS decreased in smokers(P < .01) and only the total amount of TOS decreased in non-smokers (P < .01). The concentration and total amounts of TAOC increased throughout the treatments in both smokers and non-smokers without significant changes (P > .05). The baseline OSI was higher in smokers, and it decreased only in smokers following the treatment phases (P < .01). CONCLUSIONS Smoking and periodontal inflammation were found to alter IL-17A, IL-17E, and oxidant/anti-oxidant statuses in periodontitis patients. The intra-group assessments in smokers demonstrated more apparent alterations in the oxidant/anti-oxidant statuses and IL-17A and IL-17E levels after periodontitis management.

Research paper thumbnail of Changes in IGF-I, IGFBP-3 and ghrelin levels after adenotonsillectomy in children with sleep disordered breathing

International Journal of Pediatric Otorhinolaryngology, 2009

The aim of this study was to determine the changes in insulin-like growth factor-I (IGF-I), insul... more The aim of this study was to determine the changes in insulin-like growth factor-I (IGF-I), insulin-like growth factor binding protein-3 (IGFBP-3) and ghrelin levels following adenotonsillectomy (T&A) in children with sleep disordered breathing (SDB). Methods: Forty children (mean age 4.85 AE 2.15 years) clinically diagnosed with adenotonsillar hypertrophy (ATH) related SDB were enrolled. All children underwent T&A. Serum levels of IGF-I, IGFBP-3 and ghrelin were measured before and 6 months after T&A. Results: Serum levels of IGF-I and IGFBP-3 were significantly higher after T&A (p < 0.001). Serum ghrelin levels showed a significant decrease after T&A (p < 0.001). Conclusion: Children with ATH related SDB who underwent T&A showed significant increases in IGF-I and IGFBP-3 levels indicating an increase in diurnal growth hormone secretion as well as a significant decrese in ghrelin levels indicating an increased oral food intake in the postoperative period.

Research paper thumbnail of Dikkat Eksikliği ve Hiperaktivite Bozukluğu olan Çocuklarda Semptomlar ile Serum Demir ve Ferritin Seviyeleri Arasındaki İlişki

Nöro psikiyatri Arşivi, 2011

Research paper thumbnail of Article Influence of -tocopherol and -lipoic

acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of rats

Research paper thumbnail of Association Between Symptom Profiles and Iron and Ferritine Serum Levels in Children with Attention Deficit Hyperactivity Disorder

Ahmet fiENSES, Saliha BAYKAL, Ömer BÖKE Ondokuz May›s Üniversitesi T›p Fakültesi, Çocuk ve Ergen ... more Ahmet fiENSES, Saliha BAYKAL, Ömer BÖKE Ondokuz May›s Üniversitesi T›p Fakültesi, Çocuk ve Ergen Ruh Sa¤l›¤› Bilim Dal›, Samsun, Türkiye *Ondokuz May›s Üniversitesi T›p Fakültesi, Biyokimya Bilim Dal›, Samsun, Türkiye **Ondokuz May›s Üniversitesi T›p Fakültesi, Çocuk Sa¤l›¤› ve Hastal›klar› Bilim Dal›, Samsun, Türkiye Dikkat Eksikli¤i ve Hiperaktivite Bozuklu¤u olan Çocuklarda Semptomlar ile Serum Demir ve Ferritin Seviyeleri Aras›ndaki ‹liflki