Biljana Stangeland | Oslo University Hospital Rikshospitalet (original) (raw)
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Papers by Biljana Stangeland
medRxiv (Cold Spring Harbor Laboratory), Mar 30, 2020
NAT12/NAA30 knockdown resulted in reduction of CD133+ cells as shown by flow cytometry.
Functional annotation based on the microarray analysis of NAT12/NAA30 knockdown cultures. (XLSX 2... more Functional annotation based on the microarray analysis of NAT12/NAA30 knockdown cultures. (XLSX 201 kb)
Additional qPCR statistics for Fig. 1, Fig. 3 and Fig. 5. (PDF 254 kb)
K-means clustering of the expression data for the selected NAT genes.
Oligonucleotides and microarray probes. (PDF 115 kb)
Figure S5. NAT12/NAA30 knockdown resulted in dysregulation of ribosome assembly as shown by micro... more Figure S5. NAT12/NAA30 knockdown resulted in dysregulation of ribosome assembly as shown by microarray analysis. Using the DAVID functional annotation tool we analyzed the KEGG pathway "Ribosome" where 13 genes (p=5.6E-06) were differentially regulated in KD1 and KD2. See also Supplementary File 3. Dysregulated genes are marked with red asterisks. Figure S6. NAT12/NAA30 knockdown resulted in dysregulation of the p53 pathway as shown by microarray analysis. Using the DAVID functional annotation tool we analyzed the KEGG pathway "p53" where 8 genes (p=3.1E-03) were differentially regulated in KD1 and KD2. See also Supplementary File 3. Dysregulated genes are marked with red asterisks. Figure S7. NAT12/NAA30 knockdown resulted in dysregulation of sphingolipid metabolism as shown by microarray analysis. Using the DAVID functional annotation tool we analyzed the KEGG pathway "Sphingolipid metabolism" where 6 genes (p=4.8E-03) were differentially regulated in...
List of antibodies used for western blot. (DOCX 53 kb)
survival of glioma initiating cells in vitro and attenuates tumor growth in vivo
Archives of Clinical and Medical Case Reports, 2021
Oncogene, Jan 27, 2016
Glioblastoma Multiforme (GBM) is characterized by high cancer cell heterogeneity and the presence... more Glioblastoma Multiforme (GBM) is characterized by high cancer cell heterogeneity and the presence of a complex tumor microenvironment. Those factors are a key obstacle for the treatment of this tumor type. To model the disease in mice, the current strategy is to grow GBM cells in serum-free non-adherent condition, which maintains their tumor-initiating potential. However, the so-generated tumors are histologically different from the one of origin. In this work, we performed high-throughput marker expression analysis and investigated the tumorigenicity of GBM cells enriched under different culture conditions. We identified a marker panel that distinguished tumorigenic sphere cultures from non-tumorigenic serum cultures (high CD56, SOX2, SOX9, and low CD105, CD248, αSMA). Contrary to previous work, we found that 'mixed cell cultures' grown in serum conditions are tumorigenic and express cancer stem cell (CSC) markers. As well, 1% serum plus bFGF and TGF-α preserved the tumorig...
Experimental Cell Research, 2013
medRxiv (Cold Spring Harbor Laboratory), Mar 30, 2020
NAT12/NAA30 knockdown resulted in reduction of CD133+ cells as shown by flow cytometry.
Functional annotation based on the microarray analysis of NAT12/NAA30 knockdown cultures. (XLSX 2... more Functional annotation based on the microarray analysis of NAT12/NAA30 knockdown cultures. (XLSX 201 kb)
Additional qPCR statistics for Fig. 1, Fig. 3 and Fig. 5. (PDF 254 kb)
K-means clustering of the expression data for the selected NAT genes.
Oligonucleotides and microarray probes. (PDF 115 kb)
Figure S5. NAT12/NAA30 knockdown resulted in dysregulation of ribosome assembly as shown by micro... more Figure S5. NAT12/NAA30 knockdown resulted in dysregulation of ribosome assembly as shown by microarray analysis. Using the DAVID functional annotation tool we analyzed the KEGG pathway "Ribosome" where 13 genes (p=5.6E-06) were differentially regulated in KD1 and KD2. See also Supplementary File 3. Dysregulated genes are marked with red asterisks. Figure S6. NAT12/NAA30 knockdown resulted in dysregulation of the p53 pathway as shown by microarray analysis. Using the DAVID functional annotation tool we analyzed the KEGG pathway "p53" where 8 genes (p=3.1E-03) were differentially regulated in KD1 and KD2. See also Supplementary File 3. Dysregulated genes are marked with red asterisks. Figure S7. NAT12/NAA30 knockdown resulted in dysregulation of sphingolipid metabolism as shown by microarray analysis. Using the DAVID functional annotation tool we analyzed the KEGG pathway "Sphingolipid metabolism" where 6 genes (p=4.8E-03) were differentially regulated in...
List of antibodies used for western blot. (DOCX 53 kb)
survival of glioma initiating cells in vitro and attenuates tumor growth in vivo
Archives of Clinical and Medical Case Reports, 2021
Oncogene, Jan 27, 2016
Glioblastoma Multiforme (GBM) is characterized by high cancer cell heterogeneity and the presence... more Glioblastoma Multiforme (GBM) is characterized by high cancer cell heterogeneity and the presence of a complex tumor microenvironment. Those factors are a key obstacle for the treatment of this tumor type. To model the disease in mice, the current strategy is to grow GBM cells in serum-free non-adherent condition, which maintains their tumor-initiating potential. However, the so-generated tumors are histologically different from the one of origin. In this work, we performed high-throughput marker expression analysis and investigated the tumorigenicity of GBM cells enriched under different culture conditions. We identified a marker panel that distinguished tumorigenic sphere cultures from non-tumorigenic serum cultures (high CD56, SOX2, SOX9, and low CD105, CD248, αSMA). Contrary to previous work, we found that 'mixed cell cultures' grown in serum conditions are tumorigenic and express cancer stem cell (CSC) markers. As well, 1% serum plus bFGF and TGF-α preserved the tumorig...
Experimental Cell Research, 2013