Aylin Köseler | Pamukkale University (original) (raw)

Papers by Aylin Köseler

journalagent.com

Alkol dehidrogenaz (ADH) ve aldehid dehidrogenaz (ALDH) enzimleri etanol metabolizmas›nda rol oyn... more Alkol dehidrogenaz (ADH) ve aldehid dehidrogenaz (ALDH) enzimleri etanol metabolizmas›nda rol oynayan bafll›ca enzimlerdir. Etanolden ADH oksidasyonu ile oluflan asetaldehid, toksik ve reaktif bir metabolittir. Alkolik karaci¤er hastal›¤›n›n ve alkolle iliflkili tümörlerin patogenezinde asetaldehidin rolü oldu¤u bildirilmifltir (1). ADH1C (eski ad›yla ADH3) genetik polimorfizm gösteren bir enzimdir. Toplumumuzda sa¤l›kl› gönüllülerde ADH1C alel s›kl›klar› daha önceki bir çal›flma ile saptanm›flt›r (2). Amaç: Alkolle iliflkili bafl-boyun tümörü (epidermoid kanser) olgular›nda ADH1C genotip ve alel frekanslar›n› saptamak idi. ‹leri ça-l›flmalarda bu polimorfizmin bafl boyun tümörü (epidermoid kanser) patogenezindeki olas› rolünün ayd›nlat›lmas› planlanmaktad›r. Hastalar ve Yöntem: Bafl boyun tümörü (Epidermoid Ca) olgular›ndan kan örnekleri topland› (n= 50). Lökositlerden DNA izolasyonu yap›ld›. Yaban›l (ADH1C *1) ve mutant (ADH1C *2) alellerin s›kl›klar›n› saptamak amac›yla PCR sonras›nda endonükleaz (SspI) restriksiyonu yöntemi kullan›ld› (3). Bulgular: Genotip s›kl›klar› ADH1C *1/*1, ADH1C *1/*2 ve ADH1C *2/*2 için s›ras›yla %58 (Güven aral›¤› (GA)= 50-65), % 38 (GA= 32-44) ve %4 (GA= 2-6) idi. ADH1C *1 ve ADH1C *2 alel frekanslar› s›ras›yla % 77 (GA= 0.71-0.83) ve % 23 (GA= 0.17-0.29) olarak saptand›. Yorum: ADH1C alel frekanslar›n›n bafl-boyun tümörü (Epidermoid Ca) olgular›nda saptanmas›n›n ard›ndan halen hasta grubu ile benzer epidemiyolojik özeliklere sahip kontrol grubu bulmaya çal›fl›lmaktad›r. Kontrol grubunda benzer flekilde genotipleme ifllemi ya-p›lacak, daha sonra bulgular hasta grubuna ait bulgular ile karfl›laflt›r›lacakt›r.

Cureus, Nov 7, 2022

Background This study seeks to investigate the distribution of the angiotensin-converting enzyme ... more Background This study seeks to investigate the distribution of the angiotensin-converting enzyme (ACE) gene polymorphism and serum levels in patients with viral pneumonia and predict which polymorphism will lead to severe progression of the disease. Methodology The serum ACE levels and ACE gene polymorphisms were successfully evaluated with respect to subsequent viral pneumonia using records of 100 patients with viral pneumonia and 100 healthy controls. Results ACE serum concentration was statistically significantly elevated. ACE serum concentration with a cutoff value of ≥5,256.05 pg/mL had 85.3% sensitivity and 83.2% selectivity. In addition, patients with ACE genotype D/D were 0.08 times more likely to manifest severe lung involvement than those with I/I, and patients with the I/D genotype were 0.02 times more likely than their counterparts with I/I. The computed tomography findings of the patients revealed that ACE serum concentration was significantly effective in discriminating between mild and moderate-to-severe lung involvement. No significant difference was observed between the blood parameters and ACE genotype distributions. Conclusions I/D polymorphism likely affects the expression of the ACE gene and/or the function of the angiotensin I converting enzyme. The D/D genotype is associated with vessel wall thickness and higher blood pressure. Strong evidence was found between D/D and I/D genotypes in the patient cohort concerning genotypes and ACE serum concentration. Further analysis showed that ACE serum levels were more elevated in the D/D genotype compared to the I/D genotype in the patient cohort.

Biochemical Genetics, Jun 7, 2009

One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Deni... more One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Denizli including the high frequencies of allele 24 and 18. In Denizli province of Turkey, the most common abnormal variant is Hb D-Los Angeles with a frequency of 57.8 % of the total abnormal Hbs. The aim of this study is to identify the allele frequencies of D1S80 VNTR locus in Hb D-Los Angeles carriers in Denizli province of Turkey. We studied unrelated 36 Hb D-Los Angeles carriers residing in Denizli province of Turkey. The size range of the D1S80 VNTR locus PCR products was determined first by agarose gel electrophoresis and then by a capillary electrophoresis system. For all subjects, DNA sequencing was performed. Allele frequency, theta (k) value, and observed and expected heterozygosity were calculated using Arlequin Software version 3.11. The most common alleles were the 24 (32 %), 18 (18.1 %) and 29 (16.7 %) alleles, and frequencies of these alleles were 0.329, 0.186 and 0.171 respectively. Other observed alleles percentages were 33, 2 %. We did not observe alleles 6, 15, 27 and 35, but we observed alleles 20 and 33. Results were in Hardy-Weinberg linkage disequilibrium. Observed heterozygosity was 0.889, and expected heterozygosity was 0.847. Theta (k) value was 4.91 (95 % confidence interval limits). According to our results, we concluded that Hb D-Los Angeles carriers have different allele frequencies in D1S80 VNTR and also have their own D1S80 VNTR locus divergence.

Molecular Biology Reports, Oct 7, 2012

One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Deni... more One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Denizli including the high frequencies of allele 24 and 18. In Denizli province of Turkey, the most common abnormal variant is Hb D-Los Angeles with a frequency of 57.8 % of the total abnormal Hbs. The aim of this study is to identify the allele frequencies of D1S80 VNTR locus in Hb D-Los Angeles carriers in Denizli province of Turkey. We studied unrelated 36 Hb D-Los Angeles carriers residing in Denizli province of Turkey. The size range of the D1S80 VNTR locus PCR products was determined first by agarose gel electrophoresis and then by a capillary electrophoresis system. For all subjects, DNA sequencing was performed. Allele frequency, theta (k) value, and observed and expected heterozygosity were calculated using Arlequin Software version 3.11. The most common alleles were the 24 (32 %), 18 (18.1 %) and 29 (16.7 %) alleles, and frequencies of these alleles were 0.329, 0.186 and 0.171 respectively. Other observed alleles percentages were 33, 2 %. We did not observe alleles 6, 15, 27 and 35, but we observed alleles 20 and 33. Results were in Hardy-Weinberg linkage disequilibrium. Observed heterozygosity was 0.889, and expected heterozygosity was 0.847. Theta (k) value was 4.91 (95 % confidence interval limits). According to our results, we concluded that Hb D-Los Angeles carriers have different allele frequencies in D1S80 VNTR and also have their own D1S80 VNTR locus divergence.

Kafkas Tıp Bilimleri Dergisi, Aug 1, 2021

Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as pot... more Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as potential plant based chemotherapeutic, on U87 glioblastoma cell line. Material and Method: Cytotoxicity assays were performed by determining the cell viability of the samples with MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide). Gene expression levels of glucose transporter 1 (GLUT1), Glucose transporter 3 (GLUT3), Glucose transporter 4 (GLUT4), Hexokinase 1 (HK1) and Hexokinase 2 (HK2), multidrug resistance1 (MDR1), Cytochrome P450 Family 2 Subfamily E Member 1 (CYP2E1) and Pregnane X receptor (PXR) was analyzed in glioblastoma cells using quantitative real-time RT-PCR. Results: According to the analysis, we observed a 10% increase in the expression of Glut1, however, we did not observe a difference in Glut3 expression. For Glut4, root ethanol extract decreased its expression by 13% but shoot extracts elevated the expression levels by only 5-6%. We determined the low expression levels of HK1 and HK2 in glioblastoma compared to the control group. S.autumnalis root extract led to a slight increase in MDR1 expression. We found that the expression level of CYP2E1 was 20% lower in glioblastoma cells treated with Scilla autumnalis root and shoots extracts compared to the control group. We determined downregulation in PXR expression. Conclusion: This study may contribute significantly to the understanding of the cytotoxic effect of Scilla autumnalis. This approach may allow the possibility of Scilla autumnalis plant extract as a candidate drug for the treatment of glioblastoma.

Genetika

Inflammatory processes play an important role in the pathogenesis of heart failure. The accumulat... more Inflammatory processes play an important role in the pathogenesis of heart failure. The accumulation of unfolded proteins in the endoplasmic reticulum lumen and the unfolded protein response signal path is activated. The IL-1? gene is located in the Chromosome 2.q14 region. -31 and -511 single nucleotide polymorphisms (SNPs) were detected in the IL-1?promoter region. These two SNPs affect IL-1expression. This study aims to investigate the presence of IL-1?(-511 C / T) and IL-1? (-31 T / C) gene polymorphisms and the relationship between ER stress markers and inflammatory markers. Patients who applied to the department of emergency medicine with the findings of acute decompensated heart failure. Polymorphic sites of theIL-1? gene were determined by DNA sequencing. In all study, individuals with IL-1?(-31 T / C) T allele have higher serum PERK, GRP-78, CHOP and CRP levels median values than individuals with IL-1?(-31 T/C) C allele (p = 0.0001, p = 0.002, p = 0.002 and p =0.011, respec...

An ancient anatomic variation: bilateral elongated

Journal of Biotechnology, 2018

Pfeiffer Syndrome is the result of an autosomal dominant genetic defect which is most commonly as... more Pfeiffer Syndrome is the result of an autosomal dominant genetic defect which is most commonly associated with mutations in fibroblast growth factor receptor 1 (FGFR1) and fibroblast growth factor receptor 2 (FGFR2) genes. The clinical features of Pfeiffer Syndrome include variable degrees of craniosynostosis, ocular hypertelorism,

Life Sciences, 2021

Covid-19 infection was declared a global pandemic by WHO on March 11, 2020. GRP78 protein is know... more Covid-19 infection was declared a global pandemic by WHO on March 11, 2020. GRP78 protein is known to be involved in the intrusion of numerous viruses. Our current study tries to provide some insight into the variation of GRP78 protein levels in patients with Covid-19 (−) pneumonia, Covid-19 (+) pneumonia, and CT negative Covid-19 infection in comparison to the normal population through a larger number of cases. Materials and methods: 42 patients who have Covid-19 (−) pneumonia; 72 patients who have Covid-19 infection (30 pneumonia,42 CT negative patients) and 30 patient who have no known diseases (control group) have included in the study after the clinical and radiological evaluation. Serum GRP78 levels of the subjects were measured through a commercially available enzyme-linked immunosorbent assay (ELISA) kit. Results: The GRP78 level was found to be significantly higher in the Covid-19 infection group than both Covid-19 (−) pneumonia and control group (p = 0.031 and p = 0.0001, respectively).No significant difference was evident between Covid-19 (−) pneumonia, Covid-19 (+) pneumonia and CT negative Covid 19 infection groups with respect to GRP78 levels (p = 0.09). In addition, the GRP78 levels were significantly higher in the Covid-19 (−) pneumonia group than the control group (p = 0.0001). Conclusion: This prospective case-control study reveals that the serum GRP78 levels significantly increased during Covid-19 infection in comparison to both the Covid-19 (−) pneumonia and the control group. As the association between SARS-CoV-2 virus and GRP78 protein is revealed more clearly, this association may come to the fore as a therapeutic target.

The Egyptian Heart Journal

Background Diltiazem stands out as one of the front-line drugs administered in the emergency depa... more Background Diltiazem stands out as one of the front-line drugs administered in the emergency department to achieve acute rate control in patients suffering from atrial fibrillation with rapid Ventricular Response. One of the cytochrome enzymes involved in the metabolism of diltiazem is cytochrome P450 2D6 (CYP2D6). Interindividual differences can act on drug metabolism and thus drug efficacy due to the genetic polymorphism induced by the CYP2D6 enzyme. This study explores the association between the efficacy of diltiazem and the genetic polymorphism of CYP2D6 in patients with atrial fibrillation with rapid ventricular response. Results 87 out of 93 individuals with ventricular rate > 120 beats/min constituted the patient cohort. The patients were administered 0.25 mg/kg diltiazem intravenously. As a second dose, 0.35 mg/kg diltiazem was administered to patients who reportedly did not receive adequate drug efficacy. Heart rate control was considered to be achieved in patients whos...

Pamukkale Medical Journal

Purpose: Serum surfactant protein D (SP-D) plays roles in the body such as protection against vir... more Purpose: Serum surfactant protein D (SP-D) plays roles in the body such as protection against viral infection, bacterial and fungal clearance, clearance of apoptotic cells and suppression of inflammation. This study aims to examine the relationship between SP-D level and coronavirus disease (COVID-19) severity. Methods: 80 patients (30 with mild disease and 50 with severe/critical COVID-19), and 50 healthy volunteers were enrolled in the study. SP-D levels were analyzed by ELISA in serum samples. Results: The median of SP-D was found to be 2.47 (1.67-7.79) ng/ml in mild disease and 5.65 (3.09-16.55) ng/ml in severe/critical disease groups, while 2.89 (10.8-6.24) ng/ml in the healthy controls. The differences in SP-D levels between the severe/critical disease group compared to both mild disease and control groups were found statistically significant (p=0.007 and 0.001, respectively). ROC analysis showed greater AUC for the serum SP-D levels of the severe/critical COVID-19 patients co...

International Journal of Clinical Practice, 2021

Background: Annexin A1 (AnxA1) is an important endogenous glucocoticoid protein that contributes ... more Background: Annexin A1 (AnxA1) is an important endogenous glucocoticoid protein that contributes to the suppression of inflammation by limiting the production of neutrophil and pro-inflammatory cytokines. This study aims to determine the clinical predictivity value of blood AnxA1 levels in patients with mild and severe-critical pneumonia induced by COVID-19. Methods: This study employed a prospective, case-control study design and was conducted at Ankara Training and Research hospital between 10 February 2021 and 15 March 2021. A total of 74 patients (42 of whom had moderate and 32 of whom had severe/critical cases of COVID-19 disease according to World Health Organization guidelines) and 50 nonsymptomatic healthy volunteers participated in the study. Blood samples were taken from patients at the time of hospital admission, after which serum was isolated. Following the isolation of serum, AnxA1 levels were evaluated using the enzyme-linked immunosorbent assay method. Results: The serum AnxA1 levels were measured as 25.5 (18.6-38.6) ng/ml in the control group, 21.2 (14.7-32) ng/ml in the moderate disease group, and 14.8 (9.7-26.8) ng/ ml in the severe/critical disease group. Serum AnxA1 levels were significantly lower in the severe/critical disease group compared with the control and moderate disease groups (P = .01 and P = .0001, respectively). Using receiver operating characteristic analysis, a larger area under the curve (AUC) for the serum AnxA1 levels of the control group (AUC = 0.715, 95% CI = 0.626-0.803; P = .0001) was calculated compared with the COVID-19 patient group for the diagnosis of COVID-19 disease. The AnxA1 level was found to be 80% sensitive and 54.1% specific at a cutoff level of 18.5 ng/ ml for the diagnosis of COVID-19 disease. Moreover, the AnxA1 level was found to be 69.8% sensitive and 58.1% specific at a cutoff level of 17.2 ng/ml in predicting the need for intensive care unit (ICU) treatment. Conclusion: AnxA1 levels may be a beneficial biomarker in the diagnosis of COVID-19

Life Sciences, 2021

Introduction To our knowledge, the diagnostic value of the sP-Selectin level in the diagnosis of ... more Introduction To our knowledge, the diagnostic value of the sP-Selectin level in the diagnosis of COVID-19 disease has not yet been investigated. In this study, we aimed to assess this by evaluating the relationship between sP-Selectin level and the clinical severity of COVID-19 infections. Methods A total of 80 patients (50 with mild to moderate and 30 with severe COVID-19 pneumonia), and 60 non-symptomatic healthy volunteers participated in the study. Following serum isolation, sP-Selectin levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA) method. Results The serum sP-Selectin level was 1.7 ng/ml in the control group (1–3.78); 6.24 ng/ml (5.14–7.23) in mild-to-moderate pneumonia group; and 6.72 ng/ml (5.36–8.03) in the severe pneumonia group. Serum sP-Selectin levels in both mild-to-moderate pneumonia and severe pneumonia groups were found to be higher than the control group, with statistical significance (p = 0.0001 and p = 0.0001, respectively). Receiver operating characteristic analysis (ROC) showed greater area under the curve (AUC) for the serum sP-Selectin levels of the COVID-19 patients (AUC = 0.913, 95% CI = 0.857–0.969; p = 0.0001). The serum sP-Selectin level was found to be 97.5% sensitive and 80% specific at 4.125 ng/ml level for diagnosis (p = 0.0001). The serum sP-Selectin level was found to be 76.9% sensitive and 51.9% specific at the level of 6.12 ng/ml (p = 0.005) to predict the need for intensive care treatment. Conclusion This study showed that sP-Selectin can be used as a valuable biomarker in both diagnosing and predicting the need for intensive care treatment of COVID-19 infection.

Genetic Testing and Molecular Biomarkers, 2020

Aims: Nondiabetic patients were studied to determine whether modest elevations in plasma mannose ... more Aims: Nondiabetic patients were studied to determine whether modest elevations in plasma mannose may be associated with a greater incidence of coronary artery disease (CAD). Materials and Methods: Plasma insulin, mannose, glucose, hexokinase 1-2, GLUT1-GLUT4 levels, and serum mannose phosphate isomerase enzyme levels were evaluated with respect to subsequent CAD using records from 120 nondiabetic CAD patients and 120 healthy volunteers. CAD was identified from myocardial infarction and new diagnoses of angina. Results: Of 120 nondiabetic CAD patients studied, their plasma GLUT4 and HK1 levels were significantly lower than those of the control group. A significant increase in plasma mannose levels was found in the patient group compared to the control group. Conclusion: Our findings showed that elevated baseline mannose levels in plasma are associated with an increased risk of CAD over time.

In Vivo, 2019

Background/Aim: Endoplasmic reticulum (ER) stress plays a critical role in the development of car... more Background/Aim: Endoplasmic reticulum (ER) stress plays a critical role in the development of cardiac hypertrophy and heart failure. Heart failure is a crucial health problem that affects 23 million people worldwide, causes approximately 2.4 million people to be hospitalized every year in the USA, and leads to the death of more than 300,000 people. In this study, we aimed to investigate the clinical significance of ER stress markers and the predictive value of acute decompensated heart failure in patients with low ejection fraction heart failure (ADHF). Patients and Methods: This is a prospective case control study. The data included laboratory parameters pertaining to patients with ADHF in the emergency service and lipid parameters obtained during their admission to the hospital. In addition, the same parameters obtained from the control group patients with chronic heart failure (CHF) during their routine polyclinic control were recorded in the data set. Admission time to the hospital and length of hospital stay were included in the data. The levels of glucose regulated protein (GRP78), protein kinase RNA-like endoplasmic reticulum kinase (PERK), and C/EBP homologous protein (CHOP) in peripheral blood serum obtained from the patients and the control group were measured using the ELISA method. Results: Serum GRP78 concentration was lower in the HF group (p=0.003) compared to the control. The median value of serum PERK concentration in the HF group was higher than that of the control group (573 pg/ml, IQR=477.5-650 vs. 495.5 pg/ml, IQR=294-648, respectively) (p=0.001). However, there were no statistically significant differences in GRP78 and PERK serum concentrations between ADHF and CHF subgroups. Receiver operating characteristic (ROC) curve analysis showed greater area under the curve (AUC) for the serum GRP78 levels of the healthy individuals (AUC=0.748, 95% CI=0.681-0.814, p=0.0003). The serum GRP78 level was found to be 80% sensitive and 70% specific at 147.5 pg/ml (p=0.0003) for distinguishing healthy individuals from HF patients. In the ADHF subgroup, there was a moderate correlation between hospitalization time and serum CHOP concentrations (Spearman rho=0.586 and p=0.001). Conclusion: High GRP78 serum concentration may protect the patient from ER stress. In addition, the serum PERK level is high in patients with HF, whereas it is insufficient in predicting acute decompensation. CHOP may be useful in predicting the length of hospital stay in patients with ADHF. Heart failure (HF) is a clinical syndrome caused by the structural or functional impairment of ventricular filling or the ejection of blood. There is no single diagnostic test for HF, because it is a clinical diagnosis largely based on a history and physical examination of patients. HF is divided into two main groups based on the status of ejection fraction (EF) in the 2013 ACCF/AHA Heart Failure Guidelines: EFpreserved HF (HFpEF) and HF with low EF (HfrEF), EF>50

Int. Journal of Clinical Pharmacology and Therapeutics, 2010

Whether the G71R mutation contributes to the high incidence of neonatal indirect hyperbilirubinem... more Whether the G71R mutation contributes to the high incidence of neonatal indirect hyperbilirubinemia in Turkey remains unknown. In this study we screened for G71R mutation of the UGT1A1 gene in prolonged and pathological hyperbilirubinemia with unexplained etiology in newborns in Turkey. In this study, we screened the G71R mutation of the UGT1A1 gene in 70 Turkish newborn infants: 23 infants with pathologic hyperbilirubinemia, 24 infants with prolonged hyperbilirubinemia and 23 infants without pathologic and prolonged hyperbilirubinemia. Mutations were detected by non-radioactive dye terminator cycle sequencing. In these seventy infants enrolled in this study, there were 62 with G/G (88.5 %), 8 with G/R (11.5%), and none with R/R. Two (8.7%) infants in the pathologic jaundice group and 5 (20.8 %) infants in the prolonged indirect hyperbilirubinemia, one (4.3%) infant in the control group had G/R genotype. Although G/R mutation is higher in the prolonged indirect hyperbilirubinemia group, genotypic distributions among the three groups were not statistically significant. The allele frequency of the G71R mutation was found 4.3%, 10.4%, and 2.2% in the pathologic jaundice group, in the prolonged indirect hyperbilirubinemia group, and in the control group respectively. When we compared the peak serum total bilirubin concentrations of neonates according to their genotypes, the peak bilirubin concentration was higher in G/R genotype than G/G genotype. G71R mutation of UGT1A1 gene is also present in Turkish population and the presence of this mutation is associated with otherwise unexplained pathological or prolonged neonatal hyperbilirubinemia in a Turkish population.

European Addiction Research, 2012

Alcohol dependence causes serious problems which may be influenced by genetic factors associated ... more Alcohol dependence causes serious problems which may be influenced by genetic factors associated with alcohol metabolism. The aim was to investigate the allelic and genotypic difference in distribution of a polymorphism in alcohol dehydrogenase 1C gene (ADH1C) between alcohol-dependent individuals and controls, and to examine if these genotypes were associated with the age at which the patient became alcohol-dependent. We conducted a case-control study including 90 alcohol-dependent cases and 100 historic controls. The genomic DNA was isolated and the alleles were analyzed with an RFLP. The ADH1C*1 allele frequencies were 0.89 (95% CI 0.84-0.91) in controls and 0.68 (95% CI 0.61-0.74) in alcohol-dependent patients. The frequencies of the ADH1C*2 allele were 0.11 (95% CI 0.07-0.14) and 0.32 (95% CI 0.25-0.38) among controls and alcohol-dependent patients, respectively (p < 0.0001). The ADH1C*1/*1 genotype frequency was significantly higher in the control group (77%) compared to that of the alcohol-dependents (51%, p < 0.0001). The ADH1C*1/*2 genotype frequency was significantly lower in the control group (23%) compared to that of the alcohol-dependents (42%, p < 0.0001). We obtained no statistically significant difference among the ADH1C genotype groups regarding age. These findings suggest that a significantly higher presence of ADH1C*2 allele is associated with alcohol dependence in a Turkish population. Studies with other related polymorphisms are needed to more precisely estimate the association of alcohol dependence with ADH1C.

Kafkas Journal of Medical Sciences

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was declared a global pand... more Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was declared a global pandemic by WHO on March 11, 2020. Coronavirus disease (COVID-19) is the infectious disease caused by SARS-CoV-2. It is transmitted from person to person through droplets, progresses asymptomatically in 70% of the sufferers, while it may manifest itself in severe clinical conditions, ranging from viral upper respiratory tract infection to pneumonia, sepsis, septic shock, and even acute respiratory distress syndrome (ARDS), in symptomatic patients. Studies on the epidemiological and clinical features of COVID-19 have shown that these patients can develop symptoms of mild or severe acute respiratory infection. In cases with mild symptoms, upper respiratory tract symptoms such as fever, dry cough, and fatigue may develop, and abnormal chest CT findings may also be present. In cases with severe symptoms, dyspnea, diarrhea, severe pneumonia, ARDS or multiple organ failure develop, and mortality rates vary between 4.3% and 15% according to different study reports.

Kafkas Journal of Medical Sciences

Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as pot... more Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as potential plant based chemotherapeutic, on U87 glioblastoma cell line. Material and Method: Cytotoxicity assays were performed by determining the cell viability of the samples with MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide). Gene expression levels of glucose transporter 1 (GLUT1), Glucose transporter 3 (GLUT3), Glucose transporter 4 (GLUT4), Hexokinase 1 (HK1) and Hexokinase 2 (HK2), multidrug resistance1 (MDR1), Cytochrome P450 Family 2 Subfamily E Member 1 (CYP2E1) and Pregnane X receptor (PXR) was analyzed in glioblastoma cells using quantitative real-time RT-PCR. Results: According to the analysis, we observed a 10% increase in the expression of Glut1, however, we did not observe a difference in Glut3 expression. For Glut4, root ethanol extract decreased its expression by 13% but shoot extracts elevated the expression levels by only 5-6%. We determined the low expression levels of HK1 and HK2 in glioblastoma compared to the control group. S.autumnalis root extract led to a slight increase in MDR1 expression. We found that the expression level of CYP2E1 was 20% lower in glioblastoma cells treated with Scilla autumnalis root and shoots extracts compared to the control group. We determined downregulation in PXR expression. Conclusion: This study may contribute significantly to the understanding of the cytotoxic effect of Scilla autumnalis. This approach may allow the possibility of Scilla autumnalis plant extract as a candidate drug for the treatment of glioblastoma.

group (23%) compared to that of the alcohol-dependents (42%, p ! 0.0001). We obtained no statisti... more group (23%) compared to that of the alcohol-dependents (42%, p ! 0.0001). We obtained no statistically significant difference among the ADH1C genotype groups regarding age. Conclusions: These findings suggest that a significantly higher presence of ADH1C * 2 allele is associated with alcohol dependence in a Turkish population. Studies with other related polymorphisms are needed to more precisely estimate the association of alcohol dependence with ADH1C .

journalagent.com

Alkol dehidrogenaz (ADH) ve aldehid dehidrogenaz (ALDH) enzimleri etanol metabolizmas›nda rol oyn... more Alkol dehidrogenaz (ADH) ve aldehid dehidrogenaz (ALDH) enzimleri etanol metabolizmas›nda rol oynayan bafll›ca enzimlerdir. Etanolden ADH oksidasyonu ile oluflan asetaldehid, toksik ve reaktif bir metabolittir. Alkolik karaci¤er hastal›¤›n›n ve alkolle iliflkili tümörlerin patogenezinde asetaldehidin rolü oldu¤u bildirilmifltir (1). ADH1C (eski ad›yla ADH3) genetik polimorfizm gösteren bir enzimdir. Toplumumuzda sa¤l›kl› gönüllülerde ADH1C alel s›kl›klar› daha önceki bir çal›flma ile saptanm›flt›r (2). Amaç: Alkolle iliflkili bafl-boyun tümörü (epidermoid kanser) olgular›nda ADH1C genotip ve alel frekanslar›n› saptamak idi. ‹leri ça-l›flmalarda bu polimorfizmin bafl boyun tümörü (epidermoid kanser) patogenezindeki olas› rolünün ayd›nlat›lmas› planlanmaktad›r. Hastalar ve Yöntem: Bafl boyun tümörü (Epidermoid Ca) olgular›ndan kan örnekleri topland› (n= 50). Lökositlerden DNA izolasyonu yap›ld›. Yaban›l (ADH1C *1) ve mutant (ADH1C *2) alellerin s›kl›klar›n› saptamak amac›yla PCR sonras›nda endonükleaz (SspI) restriksiyonu yöntemi kullan›ld› (3). Bulgular: Genotip s›kl›klar› ADH1C *1/*1, ADH1C *1/*2 ve ADH1C *2/*2 için s›ras›yla %58 (Güven aral›¤› (GA)= 50-65), % 38 (GA= 32-44) ve %4 (GA= 2-6) idi. ADH1C *1 ve ADH1C *2 alel frekanslar› s›ras›yla % 77 (GA= 0.71-0.83) ve % 23 (GA= 0.17-0.29) olarak saptand›. Yorum: ADH1C alel frekanslar›n›n bafl-boyun tümörü (Epidermoid Ca) olgular›nda saptanmas›n›n ard›ndan halen hasta grubu ile benzer epidemiyolojik özeliklere sahip kontrol grubu bulmaya çal›fl›lmaktad›r. Kontrol grubunda benzer flekilde genotipleme ifllemi ya-p›lacak, daha sonra bulgular hasta grubuna ait bulgular ile karfl›laflt›r›lacakt›r.

Cureus, Nov 7, 2022

Background This study seeks to investigate the distribution of the angiotensin-converting enzyme ... more Background This study seeks to investigate the distribution of the angiotensin-converting enzyme (ACE) gene polymorphism and serum levels in patients with viral pneumonia and predict which polymorphism will lead to severe progression of the disease. Methodology The serum ACE levels and ACE gene polymorphisms were successfully evaluated with respect to subsequent viral pneumonia using records of 100 patients with viral pneumonia and 100 healthy controls. Results ACE serum concentration was statistically significantly elevated. ACE serum concentration with a cutoff value of ≥5,256.05 pg/mL had 85.3% sensitivity and 83.2% selectivity. In addition, patients with ACE genotype D/D were 0.08 times more likely to manifest severe lung involvement than those with I/I, and patients with the I/D genotype were 0.02 times more likely than their counterparts with I/I. The computed tomography findings of the patients revealed that ACE serum concentration was significantly effective in discriminating between mild and moderate-to-severe lung involvement. No significant difference was observed between the blood parameters and ACE genotype distributions. Conclusions I/D polymorphism likely affects the expression of the ACE gene and/or the function of the angiotensin I converting enzyme. The D/D genotype is associated with vessel wall thickness and higher blood pressure. Strong evidence was found between D/D and I/D genotypes in the patient cohort concerning genotypes and ACE serum concentration. Further analysis showed that ACE serum levels were more elevated in the D/D genotype compared to the I/D genotype in the patient cohort.

Biochemical Genetics, Jun 7, 2009

One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Deni... more One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Denizli including the high frequencies of allele 24 and 18. In Denizli province of Turkey, the most common abnormal variant is Hb D-Los Angeles with a frequency of 57.8 % of the total abnormal Hbs. The aim of this study is to identify the allele frequencies of D1S80 VNTR locus in Hb D-Los Angeles carriers in Denizli province of Turkey. We studied unrelated 36 Hb D-Los Angeles carriers residing in Denizli province of Turkey. The size range of the D1S80 VNTR locus PCR products was determined first by agarose gel electrophoresis and then by a capillary electrophoresis system. For all subjects, DNA sequencing was performed. Allele frequency, theta (k) value, and observed and expected heterozygosity were calculated using Arlequin Software version 3.11. The most common alleles were the 24 (32 %), 18 (18.1 %) and 29 (16.7 %) alleles, and frequencies of these alleles were 0.329, 0.186 and 0.171 respectively. Other observed alleles percentages were 33, 2 %. We did not observe alleles 6, 15, 27 and 35, but we observed alleles 20 and 33. Results were in Hardy-Weinberg linkage disequilibrium. Observed heterozygosity was 0.889, and expected heterozygosity was 0.847. Theta (k) value was 4.91 (95 % confidence interval limits). According to our results, we concluded that Hb D-Los Angeles carriers have different allele frequencies in D1S80 VNTR and also have their own D1S80 VNTR locus divergence.

Molecular Biology Reports, Oct 7, 2012

One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Deni... more One of our previous studies presented the allele frequencies of D1S80 VNTR locus in province Denizli including the high frequencies of allele 24 and 18. In Denizli province of Turkey, the most common abnormal variant is Hb D-Los Angeles with a frequency of 57.8 % of the total abnormal Hbs. The aim of this study is to identify the allele frequencies of D1S80 VNTR locus in Hb D-Los Angeles carriers in Denizli province of Turkey. We studied unrelated 36 Hb D-Los Angeles carriers residing in Denizli province of Turkey. The size range of the D1S80 VNTR locus PCR products was determined first by agarose gel electrophoresis and then by a capillary electrophoresis system. For all subjects, DNA sequencing was performed. Allele frequency, theta (k) value, and observed and expected heterozygosity were calculated using Arlequin Software version 3.11. The most common alleles were the 24 (32 %), 18 (18.1 %) and 29 (16.7 %) alleles, and frequencies of these alleles were 0.329, 0.186 and 0.171 respectively. Other observed alleles percentages were 33, 2 %. We did not observe alleles 6, 15, 27 and 35, but we observed alleles 20 and 33. Results were in Hardy-Weinberg linkage disequilibrium. Observed heterozygosity was 0.889, and expected heterozygosity was 0.847. Theta (k) value was 4.91 (95 % confidence interval limits). According to our results, we concluded that Hb D-Los Angeles carriers have different allele frequencies in D1S80 VNTR and also have their own D1S80 VNTR locus divergence.

Kafkas Tıp Bilimleri Dergisi, Aug 1, 2021

Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as pot... more Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as potential plant based chemotherapeutic, on U87 glioblastoma cell line. Material and Method: Cytotoxicity assays were performed by determining the cell viability of the samples with MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide). Gene expression levels of glucose transporter 1 (GLUT1), Glucose transporter 3 (GLUT3), Glucose transporter 4 (GLUT4), Hexokinase 1 (HK1) and Hexokinase 2 (HK2), multidrug resistance1 (MDR1), Cytochrome P450 Family 2 Subfamily E Member 1 (CYP2E1) and Pregnane X receptor (PXR) was analyzed in glioblastoma cells using quantitative real-time RT-PCR. Results: According to the analysis, we observed a 10% increase in the expression of Glut1, however, we did not observe a difference in Glut3 expression. For Glut4, root ethanol extract decreased its expression by 13% but shoot extracts elevated the expression levels by only 5-6%. We determined the low expression levels of HK1 and HK2 in glioblastoma compared to the control group. S.autumnalis root extract led to a slight increase in MDR1 expression. We found that the expression level of CYP2E1 was 20% lower in glioblastoma cells treated with Scilla autumnalis root and shoots extracts compared to the control group. We determined downregulation in PXR expression. Conclusion: This study may contribute significantly to the understanding of the cytotoxic effect of Scilla autumnalis. This approach may allow the possibility of Scilla autumnalis plant extract as a candidate drug for the treatment of glioblastoma.

Genetika

Inflammatory processes play an important role in the pathogenesis of heart failure. The accumulat... more Inflammatory processes play an important role in the pathogenesis of heart failure. The accumulation of unfolded proteins in the endoplasmic reticulum lumen and the unfolded protein response signal path is activated. The IL-1? gene is located in the Chromosome 2.q14 region. -31 and -511 single nucleotide polymorphisms (SNPs) were detected in the IL-1?promoter region. These two SNPs affect IL-1expression. This study aims to investigate the presence of IL-1?(-511 C / T) and IL-1? (-31 T / C) gene polymorphisms and the relationship between ER stress markers and inflammatory markers. Patients who applied to the department of emergency medicine with the findings of acute decompensated heart failure. Polymorphic sites of theIL-1? gene were determined by DNA sequencing. In all study, individuals with IL-1?(-31 T / C) T allele have higher serum PERK, GRP-78, CHOP and CRP levels median values than individuals with IL-1?(-31 T/C) C allele (p = 0.0001, p = 0.002, p = 0.002 and p =0.011, respec...

An ancient anatomic variation: bilateral elongated

Journal of Biotechnology, 2018

Pfeiffer Syndrome is the result of an autosomal dominant genetic defect which is most commonly as... more Pfeiffer Syndrome is the result of an autosomal dominant genetic defect which is most commonly associated with mutations in fibroblast growth factor receptor 1 (FGFR1) and fibroblast growth factor receptor 2 (FGFR2) genes. The clinical features of Pfeiffer Syndrome include variable degrees of craniosynostosis, ocular hypertelorism,

Life Sciences, 2021

Covid-19 infection was declared a global pandemic by WHO on March 11, 2020. GRP78 protein is know... more Covid-19 infection was declared a global pandemic by WHO on March 11, 2020. GRP78 protein is known to be involved in the intrusion of numerous viruses. Our current study tries to provide some insight into the variation of GRP78 protein levels in patients with Covid-19 (−) pneumonia, Covid-19 (+) pneumonia, and CT negative Covid-19 infection in comparison to the normal population through a larger number of cases. Materials and methods: 42 patients who have Covid-19 (−) pneumonia; 72 patients who have Covid-19 infection (30 pneumonia,42 CT negative patients) and 30 patient who have no known diseases (control group) have included in the study after the clinical and radiological evaluation. Serum GRP78 levels of the subjects were measured through a commercially available enzyme-linked immunosorbent assay (ELISA) kit. Results: The GRP78 level was found to be significantly higher in the Covid-19 infection group than both Covid-19 (−) pneumonia and control group (p = 0.031 and p = 0.0001, respectively).No significant difference was evident between Covid-19 (−) pneumonia, Covid-19 (+) pneumonia and CT negative Covid 19 infection groups with respect to GRP78 levels (p = 0.09). In addition, the GRP78 levels were significantly higher in the Covid-19 (−) pneumonia group than the control group (p = 0.0001). Conclusion: This prospective case-control study reveals that the serum GRP78 levels significantly increased during Covid-19 infection in comparison to both the Covid-19 (−) pneumonia and the control group. As the association between SARS-CoV-2 virus and GRP78 protein is revealed more clearly, this association may come to the fore as a therapeutic target.

The Egyptian Heart Journal

Background Diltiazem stands out as one of the front-line drugs administered in the emergency depa... more Background Diltiazem stands out as one of the front-line drugs administered in the emergency department to achieve acute rate control in patients suffering from atrial fibrillation with rapid Ventricular Response. One of the cytochrome enzymes involved in the metabolism of diltiazem is cytochrome P450 2D6 (CYP2D6). Interindividual differences can act on drug metabolism and thus drug efficacy due to the genetic polymorphism induced by the CYP2D6 enzyme. This study explores the association between the efficacy of diltiazem and the genetic polymorphism of CYP2D6 in patients with atrial fibrillation with rapid ventricular response. Results 87 out of 93 individuals with ventricular rate > 120 beats/min constituted the patient cohort. The patients were administered 0.25 mg/kg diltiazem intravenously. As a second dose, 0.35 mg/kg diltiazem was administered to patients who reportedly did not receive adequate drug efficacy. Heart rate control was considered to be achieved in patients whos...

Pamukkale Medical Journal

Purpose: Serum surfactant protein D (SP-D) plays roles in the body such as protection against vir... more Purpose: Serum surfactant protein D (SP-D) plays roles in the body such as protection against viral infection, bacterial and fungal clearance, clearance of apoptotic cells and suppression of inflammation. This study aims to examine the relationship between SP-D level and coronavirus disease (COVID-19) severity. Methods: 80 patients (30 with mild disease and 50 with severe/critical COVID-19), and 50 healthy volunteers were enrolled in the study. SP-D levels were analyzed by ELISA in serum samples. Results: The median of SP-D was found to be 2.47 (1.67-7.79) ng/ml in mild disease and 5.65 (3.09-16.55) ng/ml in severe/critical disease groups, while 2.89 (10.8-6.24) ng/ml in the healthy controls. The differences in SP-D levels between the severe/critical disease group compared to both mild disease and control groups were found statistically significant (p=0.007 and 0.001, respectively). ROC analysis showed greater AUC for the serum SP-D levels of the severe/critical COVID-19 patients co...

International Journal of Clinical Practice, 2021

Background: Annexin A1 (AnxA1) is an important endogenous glucocoticoid protein that contributes ... more Background: Annexin A1 (AnxA1) is an important endogenous glucocoticoid protein that contributes to the suppression of inflammation by limiting the production of neutrophil and pro-inflammatory cytokines. This study aims to determine the clinical predictivity value of blood AnxA1 levels in patients with mild and severe-critical pneumonia induced by COVID-19. Methods: This study employed a prospective, case-control study design and was conducted at Ankara Training and Research hospital between 10 February 2021 and 15 March 2021. A total of 74 patients (42 of whom had moderate and 32 of whom had severe/critical cases of COVID-19 disease according to World Health Organization guidelines) and 50 nonsymptomatic healthy volunteers participated in the study. Blood samples were taken from patients at the time of hospital admission, after which serum was isolated. Following the isolation of serum, AnxA1 levels were evaluated using the enzyme-linked immunosorbent assay method. Results: The serum AnxA1 levels were measured as 25.5 (18.6-38.6) ng/ml in the control group, 21.2 (14.7-32) ng/ml in the moderate disease group, and 14.8 (9.7-26.8) ng/ ml in the severe/critical disease group. Serum AnxA1 levels were significantly lower in the severe/critical disease group compared with the control and moderate disease groups (P = .01 and P = .0001, respectively). Using receiver operating characteristic analysis, a larger area under the curve (AUC) for the serum AnxA1 levels of the control group (AUC = 0.715, 95% CI = 0.626-0.803; P = .0001) was calculated compared with the COVID-19 patient group for the diagnosis of COVID-19 disease. The AnxA1 level was found to be 80% sensitive and 54.1% specific at a cutoff level of 18.5 ng/ ml for the diagnosis of COVID-19 disease. Moreover, the AnxA1 level was found to be 69.8% sensitive and 58.1% specific at a cutoff level of 17.2 ng/ml in predicting the need for intensive care unit (ICU) treatment. Conclusion: AnxA1 levels may be a beneficial biomarker in the diagnosis of COVID-19

Life Sciences, 2021

Introduction To our knowledge, the diagnostic value of the sP-Selectin level in the diagnosis of ... more Introduction To our knowledge, the diagnostic value of the sP-Selectin level in the diagnosis of COVID-19 disease has not yet been investigated. In this study, we aimed to assess this by evaluating the relationship between sP-Selectin level and the clinical severity of COVID-19 infections. Methods A total of 80 patients (50 with mild to moderate and 30 with severe COVID-19 pneumonia), and 60 non-symptomatic healthy volunteers participated in the study. Following serum isolation, sP-Selectin levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA) method. Results The serum sP-Selectin level was 1.7 ng/ml in the control group (1–3.78); 6.24 ng/ml (5.14–7.23) in mild-to-moderate pneumonia group; and 6.72 ng/ml (5.36–8.03) in the severe pneumonia group. Serum sP-Selectin levels in both mild-to-moderate pneumonia and severe pneumonia groups were found to be higher than the control group, with statistical significance (p = 0.0001 and p = 0.0001, respectively). Receiver operating characteristic analysis (ROC) showed greater area under the curve (AUC) for the serum sP-Selectin levels of the COVID-19 patients (AUC = 0.913, 95% CI = 0.857–0.969; p = 0.0001). The serum sP-Selectin level was found to be 97.5% sensitive and 80% specific at 4.125 ng/ml level for diagnosis (p = 0.0001). The serum sP-Selectin level was found to be 76.9% sensitive and 51.9% specific at the level of 6.12 ng/ml (p = 0.005) to predict the need for intensive care treatment. Conclusion This study showed that sP-Selectin can be used as a valuable biomarker in both diagnosing and predicting the need for intensive care treatment of COVID-19 infection.

Genetic Testing and Molecular Biomarkers, 2020

Aims: Nondiabetic patients were studied to determine whether modest elevations in plasma mannose ... more Aims: Nondiabetic patients were studied to determine whether modest elevations in plasma mannose may be associated with a greater incidence of coronary artery disease (CAD). Materials and Methods: Plasma insulin, mannose, glucose, hexokinase 1-2, GLUT1-GLUT4 levels, and serum mannose phosphate isomerase enzyme levels were evaluated with respect to subsequent CAD using records from 120 nondiabetic CAD patients and 120 healthy volunteers. CAD was identified from myocardial infarction and new diagnoses of angina. Results: Of 120 nondiabetic CAD patients studied, their plasma GLUT4 and HK1 levels were significantly lower than those of the control group. A significant increase in plasma mannose levels was found in the patient group compared to the control group. Conclusion: Our findings showed that elevated baseline mannose levels in plasma are associated with an increased risk of CAD over time.

In Vivo, 2019

Background/Aim: Endoplasmic reticulum (ER) stress plays a critical role in the development of car... more Background/Aim: Endoplasmic reticulum (ER) stress plays a critical role in the development of cardiac hypertrophy and heart failure. Heart failure is a crucial health problem that affects 23 million people worldwide, causes approximately 2.4 million people to be hospitalized every year in the USA, and leads to the death of more than 300,000 people. In this study, we aimed to investigate the clinical significance of ER stress markers and the predictive value of acute decompensated heart failure in patients with low ejection fraction heart failure (ADHF). Patients and Methods: This is a prospective case control study. The data included laboratory parameters pertaining to patients with ADHF in the emergency service and lipid parameters obtained during their admission to the hospital. In addition, the same parameters obtained from the control group patients with chronic heart failure (CHF) during their routine polyclinic control were recorded in the data set. Admission time to the hospital and length of hospital stay were included in the data. The levels of glucose regulated protein (GRP78), protein kinase RNA-like endoplasmic reticulum kinase (PERK), and C/EBP homologous protein (CHOP) in peripheral blood serum obtained from the patients and the control group were measured using the ELISA method. Results: Serum GRP78 concentration was lower in the HF group (p=0.003) compared to the control. The median value of serum PERK concentration in the HF group was higher than that of the control group (573 pg/ml, IQR=477.5-650 vs. 495.5 pg/ml, IQR=294-648, respectively) (p=0.001). However, there were no statistically significant differences in GRP78 and PERK serum concentrations between ADHF and CHF subgroups. Receiver operating characteristic (ROC) curve analysis showed greater area under the curve (AUC) for the serum GRP78 levels of the healthy individuals (AUC=0.748, 95% CI=0.681-0.814, p=0.0003). The serum GRP78 level was found to be 80% sensitive and 70% specific at 147.5 pg/ml (p=0.0003) for distinguishing healthy individuals from HF patients. In the ADHF subgroup, there was a moderate correlation between hospitalization time and serum CHOP concentrations (Spearman rho=0.586 and p=0.001). Conclusion: High GRP78 serum concentration may protect the patient from ER stress. In addition, the serum PERK level is high in patients with HF, whereas it is insufficient in predicting acute decompensation. CHOP may be useful in predicting the length of hospital stay in patients with ADHF. Heart failure (HF) is a clinical syndrome caused by the structural or functional impairment of ventricular filling or the ejection of blood. There is no single diagnostic test for HF, because it is a clinical diagnosis largely based on a history and physical examination of patients. HF is divided into two main groups based on the status of ejection fraction (EF) in the 2013 ACCF/AHA Heart Failure Guidelines: EFpreserved HF (HFpEF) and HF with low EF (HfrEF), EF>50

Int. Journal of Clinical Pharmacology and Therapeutics, 2010

Whether the G71R mutation contributes to the high incidence of neonatal indirect hyperbilirubinem... more Whether the G71R mutation contributes to the high incidence of neonatal indirect hyperbilirubinemia in Turkey remains unknown. In this study we screened for G71R mutation of the UGT1A1 gene in prolonged and pathological hyperbilirubinemia with unexplained etiology in newborns in Turkey. In this study, we screened the G71R mutation of the UGT1A1 gene in 70 Turkish newborn infants: 23 infants with pathologic hyperbilirubinemia, 24 infants with prolonged hyperbilirubinemia and 23 infants without pathologic and prolonged hyperbilirubinemia. Mutations were detected by non-radioactive dye terminator cycle sequencing. In these seventy infants enrolled in this study, there were 62 with G/G (88.5 %), 8 with G/R (11.5%), and none with R/R. Two (8.7%) infants in the pathologic jaundice group and 5 (20.8 %) infants in the prolonged indirect hyperbilirubinemia, one (4.3%) infant in the control group had G/R genotype. Although G/R mutation is higher in the prolonged indirect hyperbilirubinemia group, genotypic distributions among the three groups were not statistically significant. The allele frequency of the G71R mutation was found 4.3%, 10.4%, and 2.2% in the pathologic jaundice group, in the prolonged indirect hyperbilirubinemia group, and in the control group respectively. When we compared the peak serum total bilirubin concentrations of neonates according to their genotypes, the peak bilirubin concentration was higher in G/R genotype than G/G genotype. G71R mutation of UGT1A1 gene is also present in Turkish population and the presence of this mutation is associated with otherwise unexplained pathological or prolonged neonatal hyperbilirubinemia in a Turkish population.

European Addiction Research, 2012

Alcohol dependence causes serious problems which may be influenced by genetic factors associated ... more Alcohol dependence causes serious problems which may be influenced by genetic factors associated with alcohol metabolism. The aim was to investigate the allelic and genotypic difference in distribution of a polymorphism in alcohol dehydrogenase 1C gene (ADH1C) between alcohol-dependent individuals and controls, and to examine if these genotypes were associated with the age at which the patient became alcohol-dependent. We conducted a case-control study including 90 alcohol-dependent cases and 100 historic controls. The genomic DNA was isolated and the alleles were analyzed with an RFLP. The ADH1C*1 allele frequencies were 0.89 (95% CI 0.84-0.91) in controls and 0.68 (95% CI 0.61-0.74) in alcohol-dependent patients. The frequencies of the ADH1C*2 allele were 0.11 (95% CI 0.07-0.14) and 0.32 (95% CI 0.25-0.38) among controls and alcohol-dependent patients, respectively (p < 0.0001). The ADH1C*1/*1 genotype frequency was significantly higher in the control group (77%) compared to that of the alcohol-dependents (51%, p < 0.0001). The ADH1C*1/*2 genotype frequency was significantly lower in the control group (23%) compared to that of the alcohol-dependents (42%, p < 0.0001). We obtained no statistically significant difference among the ADH1C genotype groups regarding age. These findings suggest that a significantly higher presence of ADH1C*2 allele is associated with alcohol dependence in a Turkish population. Studies with other related polymorphisms are needed to more precisely estimate the association of alcohol dependence with ADH1C.

Kafkas Journal of Medical Sciences

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was declared a global pand... more Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was declared a global pandemic by WHO on March 11, 2020. Coronavirus disease (COVID-19) is the infectious disease caused by SARS-CoV-2. It is transmitted from person to person through droplets, progresses asymptomatically in 70% of the sufferers, while it may manifest itself in severe clinical conditions, ranging from viral upper respiratory tract infection to pneumonia, sepsis, septic shock, and even acute respiratory distress syndrome (ARDS), in symptomatic patients. Studies on the epidemiological and clinical features of COVID-19 have shown that these patients can develop symptoms of mild or severe acute respiratory infection. In cases with mild symptoms, upper respiratory tract symptoms such as fever, dry cough, and fatigue may develop, and abnormal chest CT findings may also be present. In cases with severe symptoms, dyspnea, diarrhea, severe pneumonia, ARDS or multiple organ failure develop, and mortality rates vary between 4.3% and 15% according to different study reports.

Kafkas Journal of Medical Sciences

Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as pot... more Aim: This study aimed to measure the effects of Scilla autumnalis extracts which might act as potential plant based chemotherapeutic, on U87 glioblastoma cell line. Material and Method: Cytotoxicity assays were performed by determining the cell viability of the samples with MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide). Gene expression levels of glucose transporter 1 (GLUT1), Glucose transporter 3 (GLUT3), Glucose transporter 4 (GLUT4), Hexokinase 1 (HK1) and Hexokinase 2 (HK2), multidrug resistance1 (MDR1), Cytochrome P450 Family 2 Subfamily E Member 1 (CYP2E1) and Pregnane X receptor (PXR) was analyzed in glioblastoma cells using quantitative real-time RT-PCR. Results: According to the analysis, we observed a 10% increase in the expression of Glut1, however, we did not observe a difference in Glut3 expression. For Glut4, root ethanol extract decreased its expression by 13% but shoot extracts elevated the expression levels by only 5-6%. We determined the low expression levels of HK1 and HK2 in glioblastoma compared to the control group. S.autumnalis root extract led to a slight increase in MDR1 expression. We found that the expression level of CYP2E1 was 20% lower in glioblastoma cells treated with Scilla autumnalis root and shoots extracts compared to the control group. We determined downregulation in PXR expression. Conclusion: This study may contribute significantly to the understanding of the cytotoxic effect of Scilla autumnalis. This approach may allow the possibility of Scilla autumnalis plant extract as a candidate drug for the treatment of glioblastoma.

group (23%) compared to that of the alcohol-dependents (42%, p ! 0.0001). We obtained no statisti... more group (23%) compared to that of the alcohol-dependents (42%, p ! 0.0001). We obtained no statistically significant difference among the ADH1C genotype groups regarding age. Conclusions: These findings suggest that a significantly higher presence of ADH1C * 2 allele is associated with alcohol dependence in a Turkish population. Studies with other related polymorphisms are needed to more precisely estimate the association of alcohol dependence with ADH1C .