DR MAJID R A U F AHMAD | Postgraduate Medical Institute, University of Health Sciences, Lahore (original) (raw)

Papers by DR MAJID R A U F AHMAD

Research paper thumbnail of Evaluation of Chicago sky blue stain against Potassium Hydroxide-Dimethyl Sulfoxide wet mount in the identification of dermatophytes

Journal of Fatima Jinnah Medical University, 2020

Background: Dermatophytoses infections are widespread in the developing world. The laboratory dia... more Background: Dermatophytoses infections are widespread in the developing world. The laboratory diagnosis of dermatophytes has been a challenge as it involves microscopy and trained personnel. Potassium hydroxide wet mount with dimethyl sulfoxide added is routinely used in direct microscopy. But it lacks color contrast and the hyphae may be missed on routine microscopy. The study aimed to evaluate the effectiveness of the Chicago sky blue stain against routine potassium hydroxide-dimethyl sulfoxide (KOH/DMSO) wet mount in direct microscopy. Patients and methods: The study was carried out at the Department of Microbiology, Postgraduate Medical Institute, Lahore over a period of nine months from July 2013 till March 2014. Patients of either gender regardless of age, clinically diagnosed as having dermatophytoses by dermatologists were selected for this study. Specimens from 100 patients were collected from the dermatology outdoor of a tertiary care hospital for this study. They were eva...

Research paper thumbnail of Pattern of dermatophytes among skin, hair, and nail specimens in a tertiary care hospital of Lahore

Journal of Fatima Jinnah Medical University, Apr 4, 2022

Background: Dermatophytes are considered to be the most common cause fungal of infections globall... more Background: Dermatophytes are considered to be the most common cause fungal of infections globally. The geographical distribution of these fungi varies from region to region. The aim of this study was to isolate and determine the frequency and variety of various dermatophytes from clinically suspected cases of dermatophytoses. Patients and methods: This was a descriptive study carried out at the Department of Microbiology, Post graduate Medical Institute, Lahore over a period of nine months from July 2013 till March 2014. Nonprobability convenient sampling was used. One hundred clinically suspected cases of dermatophytosis were selected for this study. Specimen of skin, hair and nail were taken from patients and were evaluated by both microscopic examination and culture. Dermatophytes were identified based on the colony morphology as well as microscopic arrangement of macroconidia and microconidia. Data was collected and entered in Statistical Package for the Social Sciences (SPSS) version 20.0. Results: Out of a total of 100 patients, 48 were male and 52 were female. The majority of cases were from the age group of 31-40 (25%) followed by less than 10 years (18%). Out of hundred patients, 59 (59%) were positive on direct microscopy with KOH wet mount. Fungal culture was positive in 56 (56%) cases. Out of these 56 positive cultures, twenty-three were identified as dermatophyte species. Trichophyton rubrum was the commonest isolate. Other dermatophyte species isolated were Trichophyton mentagrophytes, Trichophyton verrocosum, Microsporum canis, Trichophyton tonsurans, and Microsporum audonii. Conclusion: Studying the pattern of dermatophyte species is necessary as it helps in the early diagnosis and treatment of dermatomycoses. This study identified Trichophyton rubrum as the commonest etiological agent of dermatophytoses.

Research paper thumbnail of Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species

BIOMEDICA, 2020

Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment i... more Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment is started. Although direct microscopy is routinely performed but culture of dermatophytes is the gold standard. However, it takes about 4 weeks for species identification on primary media. Our aim was to compare dermatophyte test medium (DTM) as a screening medium for the isolation of dermatophytes in comparison with sabouraud dextrose agar (SDA). Methods: It was a comparative study carried out at the Department of Microbiology of Post Graduate Medical Institute, Lahore over a period of nine months. Samples were collected from one hundred patients with clinically suspected dermatophytoses after taking informed written consent. The samples were examined microscopically and then inoculated on two types of culture media, one Sabouraud dextrose agar (SDA) with added chloramphenicol, gentacin and cycloheximide and other dermatophyte test medium (DTM) with added chlortetracycline, gentacin and...

Research paper thumbnail of Evaluation of Chicago sky blue stain against Potassium Hydroxide-Dimethyl Sulfoxide wet mount in the identification of dermatophytes

Journal of Fatima Jinnah Medical University, 2020

Background: Dermatophytoses infections are widespread in the developing world. The laboratory dia... more Background: Dermatophytoses infections are widespread in the developing world. The laboratory diagnosis of dermatophytes has been a challenge as it involves microscopy and trained personnel. Potassium hydroxide wet mount with dimethyl sulfoxide added is routinely used in direct microscopy. But it lacks color contrast and the hyphae may be missed on routine microscopy. The study aimed to evaluate the effectiveness of the Chicago sky blue stain against routine potassium hydroxide-dimethyl sulfoxide (KOH/DMSO) wet mount in direct microscopy. Patients and methods: The study was carried out at the Department of Microbiology, Postgraduate Medical Institute, Lahore over a period of nine months from July 2013 till March 2014. Patients of either gender regardless of age, clinically diagnosed as having dermatophytoses by dermatologists were selected for this study. Specimens from 100 patients were collected from the dermatology outdoor of a tertiary care hospital for this study. They were eva...

Research paper thumbnail of Pattern of dermatophytes among skin, hair, and nail specimens in a tertiary care hospital of Lahore

Journal of Fatima Jinnah Medical University, Apr 4, 2022

Background: Dermatophytes are considered to be the most common cause fungal of infections globall... more Background: Dermatophytes are considered to be the most common cause fungal of infections globally. The geographical distribution of these fungi varies from region to region. The aim of this study was to isolate and determine the frequency and variety of various dermatophytes from clinically suspected cases of dermatophytoses. Patients and methods: This was a descriptive study carried out at the Department of Microbiology, Post graduate Medical Institute, Lahore over a period of nine months from July 2013 till March 2014. Nonprobability convenient sampling was used. One hundred clinically suspected cases of dermatophytosis were selected for this study. Specimen of skin, hair and nail were taken from patients and were evaluated by both microscopic examination and culture. Dermatophytes were identified based on the colony morphology as well as microscopic arrangement of macroconidia and microconidia. Data was collected and entered in Statistical Package for the Social Sciences (SPSS) version 20.0. Results: Out of a total of 100 patients, 48 were male and 52 were female. The majority of cases were from the age group of 31-40 (25%) followed by less than 10 years (18%). Out of hundred patients, 59 (59%) were positive on direct microscopy with KOH wet mount. Fungal culture was positive in 56 (56%) cases. Out of these 56 positive cultures, twenty-three were identified as dermatophyte species. Trichophyton rubrum was the commonest isolate. Other dermatophyte species isolated were Trichophyton mentagrophytes, Trichophyton verrocosum, Microsporum canis, Trichophyton tonsurans, and Microsporum audonii. Conclusion: Studying the pattern of dermatophyte species is necessary as it helps in the early diagnosis and treatment of dermatomycoses. This study identified Trichophyton rubrum as the commonest etiological agent of dermatophytoses.

Research paper thumbnail of Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species

BIOMEDICA, 2020

Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment i... more Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment is started. Although direct microscopy is routinely performed but culture of dermatophytes is the gold standard. However, it takes about 4 weeks for species identification on primary media. Our aim was to compare dermatophyte test medium (DTM) as a screening medium for the isolation of dermatophytes in comparison with sabouraud dextrose agar (SDA). Methods: It was a comparative study carried out at the Department of Microbiology of Post Graduate Medical Institute, Lahore over a period of nine months. Samples were collected from one hundred patients with clinically suspected dermatophytoses after taking informed written consent. The samples were examined microscopically and then inoculated on two types of culture media, one Sabouraud dextrose agar (SDA) with added chloramphenicol, gentacin and cycloheximide and other dermatophyte test medium (DTM) with added chlortetracycline, gentacin and...