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Papers by Chandraprakash Srinivasan

Plant Archives, An International Journal, 2024

Turmeric (Curcuma longa), valued for its medicinal properties in India, faces a threat from Colle... more Turmeric (Curcuma longa), valued for its medicinal properties in India, faces a threat from Colletotrichum capsici, causing leaf spot and yield losses of 15% to 60%. Colletotrichum capsici was isolated from infected turmeric leaves, displaying characteristic features. Trichoderma viride effectively inhibited its growth in vitro. Treatment T1R3 showed the highest inhibition (72.22% PIOC) of mycelial growth, while T1R2 exhibited the lowest (2.85cm). This study underscores Trichoderma viride's potential as a biocontrol agent against Colletotrichum capsici, offering insights for managing turmeric leaf spot and improving agricultural productivity.

Eco. Env. & Cons., 2024

Sesame (Sesamum indicum L.) is an important oilseed crop cultivated in various parts of the world... more Sesame (Sesamum indicum L.) is an important oilseed crop cultivated in various parts of the world. Alternaria sesami is a devastating fungal pathogen that causes significant yield losses in sesame crops worldwide. The survey conducted revealed a maximum disease incidence of 55.00 percent at Namakkal. Alternaria sesami was isolated from the leaf blight-infected sesame leaves. Trichoderma viride recorded significant results against Alternaria sesami in vitro. T1R2 showed the highest percent inhibition over control (PIOC) (77.77%) on mycelial growth of Alternaria sesami. 72.22 and 67.77 percentages of inhibition were recorded in T1R1 and T1R3, respectively.

Eco. Env. & Cons. 28 (December Suppl. Issue) : 2022; pp. (S252-S256) Copyright@ EM International ISSN 0971–765X, 2022

Oyster mushrooms are considered an important health food all over the world. There are various va... more Oyster mushrooms are considered an important health food all over the world. There are various varieties of mushrooms cultivated by the growers. The experiment at Ramakrishna Mission Vivekananda Educational and Research Institute, Faculty Centre for Agricultural Education and Research, Coimbatore was designed to assess the performance of the oyster mushroom Pleurotus sajar-caju in various substrates and to identify the best substrates for cultivation. Six different substrates (viz., paddy straw, sugarcane bagasse, teak leaves, forest waste, turf waste, and bamboo leaves) were used for oyster mushroom production. T3-Forest waste and T1-Sugarcane Bagasse had the shortest spawn running durations (20 and 20.3 days, respectively), followed by T2 and T5-teak and bamboo leaves (22.3 days). Among the six substrates, T0-Paddy straw (570 g) produced a significantly higher yield, followed by T4-Turf grass (467 g).

International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Special Issue-7 pp. 3066-3080, 2018

Xanthomonas oryzae pv. oryzae (Xoo) is the pathogen causing bacterial leaf blight (BLB) disease i... more Xanthomonas oryzae pv. oryzae (Xoo) is the pathogen causing bacterial leaf blight
(BLB) disease in paddy crop and its occurrence was observed in severe form in paddy
fields of Madurai during Kharif and Rabi seasons of 2016. Survey on the severity of
BLB disease was conducted in 11 different places of Madurai district. From the BLB
infected samples collected in these areas, 11 isolates of the pathogen, Xoo were isolated
and characterised by standard morphological and molecular methods. All the 11
isolates of Xoo were virulent, by expressing differences in disease intensity on
susceptible paddy cultivar, ADT 39 inoculated with clip inoculation method. The
isolate XooM1 was found to be virulent and used in further study. Bacteriophages were
isolated from the paddy field flood water and BLB infected leaf samples. Totally five
isolates of bacteriophage was recovered by three successive single plaque isolations by
using Xoo as host. Among these five, Xoo Bp (MDU 1) was found to be highly
effective in relative plaque forming efficiency in vitro and recovering viable count of
Xoo M1 after 120 min of incubation. Then it was examined using a Transmission
Electron Microscope (TEM) under 20 nm and 50 nm magnification and the size of the
phage head was 61.8 x 55.7 nm and length of tail was 95.9 nm. Phage Xoo Bp MDU1
was formulated in different protectants viz., pregelatinised corn flour (PCF – 0.5% and
0.25%), Skim milk (0.05% and 0.75%), Sucrose (0.5%), Gluten (1%), Gelatin (0.5%)
and in combinations. Likewise 11 formulations were prepared in combinations of the
protectants and tested for their effect on BLB disease management. Among these 11
formulations, phage titer was found to be highest in skim milk (0.5%) + sucrose (0.5%)
formulation and the phage titre was 9.85 x1010 PFU/ml. The skim milk (0.5%) +
sucrose (0.5%) formulation recorded the lowest PDI of 33.58 with a percent reduction
over control (PROC) of 40.73. Under artificially inoculated condition, seed treatment
with skim milk formulation of Xoo Bp (MDU1) @ 200ml/kg and foliar spray @ 20%
recorded PDI of 37.5 and 24.40 PROC.

The Pharma Innovation Journal, 2022

The performance of the oyster mushroom Pleurotus florida on various substrates was evaluated, as ... more The performance of the oyster mushroom Pleurotus florida on various substrates was evaluated, as well as the best substrates for mushroom cultivation. Six different substrates, viz., banana leaves, sugarcane bagasse, teak leaves, bamboo leaves, coconut husk fibre, and paddy straw, were used for oyster mushroom production. The banana leaves (438.95 g) produced a significantly higher yield next to paddy straw (455.54g).

International Journal of Agriculture Sciences, 2022

Oyster mushrooms are considered an important health food all over the world. There are various va... more Oyster mushrooms are considered an important health food all over the world. There are various varieties of mushrooms cultivated by the growers. The main objective of this study is to identify the high-yielding variety of oyster mushrooms. In this experiment, the spawn of various varieties of oyster mushrooms was inoculated in paddy straw and kept in a mushroom cultivation shed. The investigation shows that the highest yield (974.05 g) was recorded in Pleurotus florida with a biological efficiency of 97.40%, followed by Pleurotus sajar-caju with an 825.28 g yield and a biological efficiency of 82.53%. Pleurotus djamor recorded a low yield (552.30 g) with 55.23% biological efficiency.

International Journal of Agriculture Sciences, 2022

Oyster mushrooms are considered an important health food all over the world. There are various va... more Oyster mushrooms are considered an important health food all over the world. There are various varieties of mushrooms cultivated by the growers. The main objective of this study is to identify the high-yielding variety of oyster mushrooms. In this experiment, the spawn of various varieties of oyster mushrooms was inoculated in paddy straw and kept in a mushroom cultivation shed. The investigation shows that the highest yield (974.05 g) was recorded in Pleurotus florida with a biological efficiency of 97.40%, followed by Pleurotus sajar-caju with an 825.28 g yield and a biological efficiency of 82.53%. Pleurotus djamor recorded a low yield (552.30 g) with 55.23% biological efficiency.

Plant Archives, An International Journal, 2024

Turmeric (Curcuma longa), valued for its medicinal properties in India, faces a threat from Colle... more Turmeric (Curcuma longa), valued for its medicinal properties in India, faces a threat from Colletotrichum capsici, causing leaf spot and yield losses of 15% to 60%. Colletotrichum capsici was isolated from infected turmeric leaves, displaying characteristic features. Trichoderma viride effectively inhibited its growth in vitro. Treatment T1R3 showed the highest inhibition (72.22% PIOC) of mycelial growth, while T1R2 exhibited the lowest (2.85cm). This study underscores Trichoderma viride's potential as a biocontrol agent against Colletotrichum capsici, offering insights for managing turmeric leaf spot and improving agricultural productivity.

Eco. Env. & Cons., 2024

Sesame (Sesamum indicum L.) is an important oilseed crop cultivated in various parts of the world... more Sesame (Sesamum indicum L.) is an important oilseed crop cultivated in various parts of the world. Alternaria sesami is a devastating fungal pathogen that causes significant yield losses in sesame crops worldwide. The survey conducted revealed a maximum disease incidence of 55.00 percent at Namakkal. Alternaria sesami was isolated from the leaf blight-infected sesame leaves. Trichoderma viride recorded significant results against Alternaria sesami in vitro. T1R2 showed the highest percent inhibition over control (PIOC) (77.77%) on mycelial growth of Alternaria sesami. 72.22 and 67.77 percentages of inhibition were recorded in T1R1 and T1R3, respectively.

Eco. Env. & Cons. 28 (December Suppl. Issue) : 2022; pp. (S252-S256) Copyright@ EM International ISSN 0971–765X, 2022

Oyster mushrooms are considered an important health food all over the world. There are various va... more Oyster mushrooms are considered an important health food all over the world. There are various varieties of mushrooms cultivated by the growers. The experiment at Ramakrishna Mission Vivekananda Educational and Research Institute, Faculty Centre for Agricultural Education and Research, Coimbatore was designed to assess the performance of the oyster mushroom Pleurotus sajar-caju in various substrates and to identify the best substrates for cultivation. Six different substrates (viz., paddy straw, sugarcane bagasse, teak leaves, forest waste, turf waste, and bamboo leaves) were used for oyster mushroom production. T3-Forest waste and T1-Sugarcane Bagasse had the shortest spawn running durations (20 and 20.3 days, respectively), followed by T2 and T5-teak and bamboo leaves (22.3 days). Among the six substrates, T0-Paddy straw (570 g) produced a significantly higher yield, followed by T4-Turf grass (467 g).

International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Special Issue-7 pp. 3066-3080, 2018

Xanthomonas oryzae pv. oryzae (Xoo) is the pathogen causing bacterial leaf blight (BLB) disease i... more Xanthomonas oryzae pv. oryzae (Xoo) is the pathogen causing bacterial leaf blight
(BLB) disease in paddy crop and its occurrence was observed in severe form in paddy
fields of Madurai during Kharif and Rabi seasons of 2016. Survey on the severity of
BLB disease was conducted in 11 different places of Madurai district. From the BLB
infected samples collected in these areas, 11 isolates of the pathogen, Xoo were isolated
and characterised by standard morphological and molecular methods. All the 11
isolates of Xoo were virulent, by expressing differences in disease intensity on
susceptible paddy cultivar, ADT 39 inoculated with clip inoculation method. The
isolate XooM1 was found to be virulent and used in further study. Bacteriophages were
isolated from the paddy field flood water and BLB infected leaf samples. Totally five
isolates of bacteriophage was recovered by three successive single plaque isolations by
using Xoo as host. Among these five, Xoo Bp (MDU 1) was found to be highly
effective in relative plaque forming efficiency in vitro and recovering viable count of
Xoo M1 after 120 min of incubation. Then it was examined using a Transmission
Electron Microscope (TEM) under 20 nm and 50 nm magnification and the size of the
phage head was 61.8 x 55.7 nm and length of tail was 95.9 nm. Phage Xoo Bp MDU1
was formulated in different protectants viz., pregelatinised corn flour (PCF – 0.5% and
0.25%), Skim milk (0.05% and 0.75%), Sucrose (0.5%), Gluten (1%), Gelatin (0.5%)
and in combinations. Likewise 11 formulations were prepared in combinations of the
protectants and tested for their effect on BLB disease management. Among these 11
formulations, phage titer was found to be highest in skim milk (0.5%) + sucrose (0.5%)
formulation and the phage titre was 9.85 x1010 PFU/ml. The skim milk (0.5%) +
sucrose (0.5%) formulation recorded the lowest PDI of 33.58 with a percent reduction
over control (PROC) of 40.73. Under artificially inoculated condition, seed treatment
with skim milk formulation of Xoo Bp (MDU1) @ 200ml/kg and foliar spray @ 20%
recorded PDI of 37.5 and 24.40 PROC.

The Pharma Innovation Journal, 2022

The performance of the oyster mushroom Pleurotus florida on various substrates was evaluated, as ... more The performance of the oyster mushroom Pleurotus florida on various substrates was evaluated, as well as the best substrates for mushroom cultivation. Six different substrates, viz., banana leaves, sugarcane bagasse, teak leaves, bamboo leaves, coconut husk fibre, and paddy straw, were used for oyster mushroom production. The banana leaves (438.95 g) produced a significantly higher yield next to paddy straw (455.54g).

International Journal of Agriculture Sciences, 2022

Oyster mushrooms are considered an important health food all over the world. There are various va... more Oyster mushrooms are considered an important health food all over the world. There are various varieties of mushrooms cultivated by the growers. The main objective of this study is to identify the high-yielding variety of oyster mushrooms. In this experiment, the spawn of various varieties of oyster mushrooms was inoculated in paddy straw and kept in a mushroom cultivation shed. The investigation shows that the highest yield (974.05 g) was recorded in Pleurotus florida with a biological efficiency of 97.40%, followed by Pleurotus sajar-caju with an 825.28 g yield and a biological efficiency of 82.53%. Pleurotus djamor recorded a low yield (552.30 g) with 55.23% biological efficiency.

International Journal of Agriculture Sciences, 2022

Oyster mushrooms are considered an important health food all over the world. There are various va... more Oyster mushrooms are considered an important health food all over the world. There are various varieties of mushrooms cultivated by the growers. The main objective of this study is to identify the high-yielding variety of oyster mushrooms. In this experiment, the spawn of various varieties of oyster mushrooms was inoculated in paddy straw and kept in a mushroom cultivation shed. The investigation shows that the highest yield (974.05 g) was recorded in Pleurotus florida with a biological efficiency of 97.40%, followed by Pleurotus sajar-caju with an 825.28 g yield and a biological efficiency of 82.53%. Pleurotus djamor recorded a low yield (552.30 g) with 55.23% biological efficiency.