shilpa verekar | Piramal Enterprises Limited (original) (raw)

Papers by shilpa verekar

Mycopathologia, Oct 12, 2007

Eighty soil samples were collected from various sites of Bahrain and screened for presence of ker... more Eighty soil samples were collected from various sites of Bahrain and screened for presence of keratinophilic fungi using hair baiting techniques for isolation. Thirty-six isolates were recovered and identified. The cultures were identified using macroand micromorphological features. Their identification was also confirmed by the BLAST search of sequences of ITS1-5.8S-ITS2 rDNA region against the NCBI/Gene bank data and compared with deposited sequences for confirmation. Eight species of five genera were isolated viz. Aphanoascus fulvuscence (8.75%), Aphanoascus punsolae (20.00%), Chrysosporium indicum (2.50%), Chrysosporium tropicum (2.50%), Chrysosporium zonatum (3.75%), Spiromastix warcupii (1.25%), Microsporum gypseum (3.75%), and Trichophyton mentagrophytes (2.50%). In conclusion, our study indicates that keratinophilic fungi do occur in the various soils of Bahrain. Moreover, the narrow diversity and low density of keratinophilic fungi in the investigated soils is expected and is emblematic to other hot arid environments.

Science Publishers eBooks, Jan 3, 2009

Springer eBooks, 2014

This review covers substantially the cytotoxic compounds isolated from endophytic fungi from terr... more This review covers substantially the cytotoxic compounds isolated from endophytic fungi from terrestrial and mangrove plants during 2009–2012. Endophytes living asymptomatically within plant tissues have been found in almost all plants studied to date. Many of the compounds reported here were originally isolated from plants, then from endophytic fungi, whereas some are exclusively isolated from endophytic fungi. The anticancer activities in this review are from the published cytotoxicity against specific cancer cell lines. Development of these natural compounds is based on their cytotoxic activity profiles, chemical structures, and potential structure–activity relationship deduced from the biochemical and cytotoxic studies.

CRC Press eBooks, Jul 15, 2021

Journal De Mycologie Medicale, Jun 1, 2021

A total of 70 feathers samples of Emu (Dromaius novaehollandiae) were collected from 7 Emu farms ... more A total of 70 feathers samples of Emu (Dromaius novaehollandiae) were collected from 7 Emu farms situated at two districts (Raigad and Thane) of Maharashtra (India) and screened for resident keratinophilic fungi. Among them, 44 isolates were recovered and identified by evaluating characteristic macro- and micro-morphological features. Further gene products corresponding to the ITS1-5.8S-ITS2 rDNA region from all isolates were amplified and sequenced. Homology search was performed using BLAST program against non-redundant nucleotide database, and significantly matched DNA sequences deposited to the NCBI Gene Bank for reference purposes. Eight identified fungal species belongs to 7 different genera named as Aphanoascus terreus Ac_MW577456 (21.43%), Microsporum gypseum Ac_MW580920 (14.29%), Ctenomyces serratus Ac_MW577459 (10.0%), Uncinocarpus orissi Ac_MW577461 (5.17%), Aphanoascus verrucosus Ac_MW577458 (4.29%), Gymnascella dankaliensis Ac_MW577460 (2.86%), Gymnoascoideus petalosporus Ac_MW577462 (2.86%) and Arthroderma tuberculatum Ac_MW577457 (1.43%).

Bioorganic Chemistry, Apr 1, 2019

Aggregation/misfolding of α-synuclein and βA4 proteins cause neuronal cell death (NCD) associated... more Aggregation/misfolding of α-synuclein and βA4 proteins cause neuronal cell death (NCD) associated with Parkinson's and Alzheimer's disease. It has been suggested that a heat shock protein-90 (Hsp90) inhibitor can prevent NCD by activating the heat shock transcription factor-1 which, in turn, upregulates molecular chaperones such as Hsp70 that targets aggregated/misfolded proteins for refolding/degradation. We have isolated radicicol, an Hsp90 inhibitor, from a fungus occurring in the crevices of marble rocks of Central India. Radicicol, which was found to be a strong antioxidant, was tested for its ability to rescue yeast cells from death induced by expression of wild-type α-synuclein, its more toxic A53T mutant, and βA4. It effectively overcomes wild-type/ mutant α-synuclein mediated yeast cell death, concomitantly diminishes ROS levels, reverses mitochondrial dysfunction and prevents nuclear DNA-fragmentation, a hallmark of apoptosis. Surprisingly however, radicicol is unable to rescue yeast cells from death triggered by expression of secreted βA4. Moreover, although radicicol acts as an antioxidant it fails to prevent yeast cell death inflicted by the proapoptotic protein, Bax. Our results indicate that radicicol specifically targets aggregated/misfolded α-synuclein's toxicity and opens up the possibility of using multiple yeast assays to screen natural product libraries for compounds that would unambiguously target α-synuclein aggregation/misfolding.

Springer eBooks, Dec 13, 2011

... Chapter 27 Fungi: A Potential Source of Anti-inflammatory Compounds Sunil Kumar Deshmukh , Sh... more ... Chapter 27 Fungi: A Potential Source of Anti-inflammatory Compounds Sunil Kumar Deshmukh , Shilpa A. Verekar , Giridharan Periyasamy , and BN Ganguli Page 2. 614 SK Deshmukh et al. ... B inhibitor Johnson et al. ( 1943 ), Anitha and Murugesan ( 2005 ), Brian ( 1946 ...

Microbiology research, Oct 3, 2011

Alkaline soils commonly called as Usar soil having pH 7.5 to 11.0 is commonly found in regions ha... more Alkaline soils commonly called as Usar soil having pH 7.5 to 11.0 is commonly found in regions having poor drainage and little percolation. These soils occupy vast tracts of barren lands in the northern parts of India. These areas are frequently visited by man and animals, however there are no reports on the occurrence of keratinophilic fungi from the Usar soils. The purpose of this research was to study the occurrence of keratinophilic fungi in the alkaline Usar soil. To investigate the occurrence of soil keratinophilic fungi from Usar soil, 120 samples were collected from five districts of Uttar Pradesh viz. Lucknow, Bareilly, Azamgarh, Balia and Pratapgarh. Keratinophilic fungi were isolated by the hair baiting techniques using human hair as keratin bait. The cultures were identified using macro-and micro morphological features. Eighty-tow strains of keratinophilic fungi were recovered from 120 (68.33%) soil samples. The isolated fungi were composed of ten species of five genera viz. Chrysosporium indicum (19.16%), Microsporum gypseum (12.5%), Chrysosporium tropicum (10.0%), Chrysosporium pannicola (7.5%), Trichophyton terrestre (5.83%), Chrysosporium lucknowense (4.16%), Chrysosporium state of Ctenomyces serratus (3.33%), Gymnascella dankailensis (3.33%), Gymnoascus reessii (1.66 %) and Trichophyton mentagrophytes (1.66 %). The presence of keratinophilic fungi in the Usar soils of Uttar Pradesh, India indicates that these fungi do occur at higher alkalinity.

Springer eBooks, 2017

This chapter discusses the occurrence of keratinophilic fungi in different habitats wherein the p... more This chapter discusses the occurrence of keratinophilic fungi in different habitats wherein the population of these fungi is expected to occur: air, soil from various environments like gardens, caves, cold and saline habitat. The ability to degrade the keratinic material and mechanism of action is discussed. Importance of these fungi in petroleum hydrocarbon degradation is discussed. Various metabolites with antibacterial, antifungal, antitumor and related compounds produced by this group of fungi are documented. Examples of synthesis of silver nanoparticles are also included. The need for an extensive survey of keratinophilic fungi from unexplored substrates and habitats and biochemical potentialities is highlighted. It is concluded that fungi other than dermatophytes can be opportunistic pathogens. The need for a culture collection of this group of fungi is also highlighted.

Microbiology research, Mar 12, 2012

The parks of Mumbai are frequently visited by local residents every morning and evening. However,... more The parks of Mumbai are frequently visited by local residents every morning and evening. However, there are no reports on the occurrence of keratinophilic fungi in these areas. The purpose of this research was to study the occurrence of keratinophilic fungi in the public parks of Mumbai. One hundred soil samples were collected from five public parks: Kamla Nehru Park, Powai Garden, CD Deshmukh Garden, Five Gardens and Chota Kashmir. Keratinophilic fungi were isolated by the hair baiting technique using human hair as keratin bait. The cultures were identified using macroand micro-morphological features. Identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/Genbank data and compared with deposited sequences. The ability of these fungi to use human hair was also evaluated by release of protein in liquid media. A total of 75 strains of keratinophilic fungi were recovered from 100 (75.0%) soil samples. The isolated fungi were composed of eleven species of eight genera: Arthrographis kalrae, Auxarthron conjugatum, Chrysosporium indicum, C. queenslandicum, C. zonatum, Gymnascella dankaliensis, G. hyalinospora, Microsporum gypseum (15.0%), Myriodontium keratinophilum, Trichophyton mentagrophytes and Uncinocarpus reesii. These fungi can release 148.8-307.6 μg/mL protein in liquid media when grown on human hair in shake flask culture and also decompose 16.2-38.6% of human hair after four weeks of incubation. Our study indicates that keratinophilic fungi are to be found in the soils of various public parks in Mumbai and that human hair can be a source of pathogenic fungi.

Journal De Mycologie Medicale, Sep 1, 2018

Iet Nanobiotechnology, Sep 22, 2017

The study explored biological synthesis of metallic silver nanoparticles (AgNPs) from the less ex... more The study explored biological synthesis of metallic silver nanoparticles (AgNPs) from the less explored non‐pathogenic coprophilous fungus, sterile mycelium, PM0651419 and evaluates the antimicrobial efficacy of biosynthesised AgNPs when impregnated in wound fabrics and in combination with six antimicrobial agents. AgNPs alone proved to be potent antibacterial agents and in combination they enhanced the antibacterial activity and spectrum of antibacterials used in the study against a microbiologically diverse battery of Gram positive, Gram negative and multidrug‐resistant bacteria. AgNPs impregnated on the wound dressings established their antibacterial activity by significantly reducing the bacterial load of pathogenic bacteria like Staphylococcus aureus and Bacillus subtilis e stablishing potential as effective antimicrobial wound dressings for treatment of polymicrobial wound infections. This study presents the first report on the potential of biosynthesis of AgNPs from the under explored class of coprophilous fungi. Their promise to be used in wound dressings and as potent antibacterials alone and in combination is evaluated

Frontiers in bioscience, 2012

Introduction 3. Antifungal compounds produced by endophytic fungi 3.1. Compounds produced by coel... more Introduction 3. Antifungal compounds produced by endophytic fungi 3.1. Compounds produced by coelomycetes 3.2. Compounds produced by ascomycetes 3.3. Compounds produced by hyphomycetes 3.4. Compounds produced by unidentified fungus 4. Volatile organic compounds from endophytic fungus 5. Outlook 6. Acknowledgment 7. References Colletotrichum gloeosporioides Cryptocarya mandioccana Cis-4-hydroxy-6-deoxyscytalone (64), (4R)-4,8-dihydroxy-alpha-tetralone (65) Exophiala sp. Adenocarpus foliolosus Exochromone (66) Cryptosporiopsis quercina Tripterigeum wilfordii Cryptocandin A (67) Cryptosporiopsis sp. Pinus sylvestris Echinocandins A (68), B (69), D (70), H (71) Pezicula sp. Fagus sylvatica Echinocandins A (68), B (69), D (70), H (71) Cryptosporiopsis quercina Phleum pratense Cryptocin (72) Hormonema sp. (ATCC 74360) Juniperus communis Enfumafungin (73) Epichloe typhina Phleum pratense Epichlicin (74) Edenia gomezpompae Callicarpa acuminata Preussomerin EG1 (75), Preussomerin EG2 (76), Preussomerin EG3 (77) Endophytic strain E99297 Cistus salvifolius 5-(1,3-Butadien-1-yl)-3-(propen-1-yl)-2 (5H)-furanone (78) Botryosphaeria rhodina Bidens pilosa Botryorhodine A (79), B (80), C (81), D (82) Dinemasporium strigosum Calystegia sepium Dinemasone A (83), B (84), C (85) Chaetomium globosum Ginkgo biloba Chaetoglobosin A (86), C (87) Xylaria sp. PSU-D14 Garcinia dulcis Sordaricin (88) Xylaria sp. F0010 Abies holophylla Griseofulvin (89), 7-dechlorogriseofulvin (90) PSU-N24. Garcinia nigrolineata Griseofulvin (89) Xylaria sp. Palicourea marcgravii 2-hexyl-3-methylbutanodioic acid (91), Cytochalasin D (92) Xylaria sp. Ginkgo biloba 7-amino-4-methylcoumarin (93) Penicillium paxilli PSU-A71 Garcinia atroviridis Penicillone (94), Pyrenocine A (95), Pyrenocine B (96) Verticillium sp. Rehmannia glutinosa 2,6-dihydroxy-2-methyl-7-(prop-1E-enyl)-1-benzofuran-3 (2H)-one (97), Massariphenone (98), Ergosterol peroxide (99) Arthrinium phaeospermum Unidentified grass Arthrichitin (100) Nigrospora sp. YB-141 Azadirachta indica Solanapyrone C (101), Solanapyrone N (102), Solanapyrone O (103), Nigrosporalactone (104), Phomalactone (105) Trichoderma harzianum Llexcornuta Lindl Trichodermin (106) Sr. No Fungus Plant Source Compounds isolated Nodulisporium sp. Erica arborea Nodulisporin D (107), E (108), F (109), (3S,4S,5R)-2,4,6-trimethyloct-6-ene-3,5-diol (110), 5-hydroxy-2-hydroxymethyl-4H-chromen-4-one (111), 3-(2,3-dihydroxyphenoxy)-butanoic acid (112), Benzene 1,2,3 triol (113) Aspergillus clavatus and Paecilomyces sp. Taxus mairei and Torreya grandis Brefeldin A (114) Eupenicillium brefeldianum Arisaema erubescens Brefeldin A (114)

Mycopathologia, Oct 31, 2016

Seventy-eight soil samples were collected from the various locations in the vicinity of Kaziranga... more Seventy-eight soil samples were collected from the various locations in the vicinity of Kaziranga National Park (Assam), India, during April to October 2009 and screened for the presence of keratinophilic fungi using the hair baiting techniques for isolation. Thirty-nine isolates were recovered and identified by recognition of their macro-and micromorphological features. Their identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/GenBank data and compared with deposited sequences for identification purpose. Eleven species related to seven genera were recorded viz. Aphanoascus durus (1.28%), Arthroderma tuberculatum (3.84%), Arthroderma corniculatum (1.28%), Chrysosporium indicum (16.66%), C. tropicum (3.84%), Ctenomyces serratus (5.12%), Keratinophyton punsolae (1.28%), Microsporum appendiculatum (1.28%), Microsporum gypseum complex (11.53%), Trichophyton mentagrophytes (11.28%) and T. terrestre (2.56%).

Mycoses, Apr 6, 2010

Fifty-three soil samples were collected from various sites in the vicinity of Vedanthangal Water ... more Fifty-three soil samples were collected from various sites in the vicinity of Vedanthangal Water Bird Sanctuary and screened for the presence of keratinophilic fungi using the hair baiting techniques for isolation. Twenty-eight isolates were recovered and identified by recognition of their macro- and micromorphological features. Seven species related to five genera were recorded viz. Auxarthron conjugatum (1.89%), Chrysosporium fluviale (3.77%), Chrysosporium indicum (20.75%), Chrysosporium tropicum (7.55%), Chrysosporium state of Ctenomyces serratus (5.66%), Gymnoascus petalosporus (1.89%) and Microsporum gypseum complex (11.32%). The study shows that migratory birds harbour a variety of keratinophiles and may be a potential source of transfer of these fungi from one location to another.

Mycopathologia, Oct 1, 2006

Thirty-two soil samples were collected from six sites in the vicinity of Lonar lake, a meteorite ... more Thirty-two soil samples were collected from six sites in the vicinity of Lonar lake, a meteorite crater and screened for presence of keratinophilic fungi using hair baiting techniques for isolation. Seventeen isolates were recovered and identified. The cultures were identified by recognition of their macro- and micromorphological features. Their identification was also confirmed by the BLAST search of sequences of ITS1-5.8S-ITS2 rDNA region against the NCBI/Gene bank data and compared with deposited sequences for identification purpose. Seven species of four genera were isolated viz. Aphanoascus durus (9.38%), Aphanoascus punsolae (3.125%), Auxarthron kuehnii (3.125%), Chrysosporium indicum (25.00%), Chrysosporium tropicum (3.125%), Chrysosporium sp. (3.125%), Chrysosporium state of Ctenomyces serratus (6.25%).

Czech Mycology, Aug 10, 2006

Results of a preliminary study of keratinophilic fungi from soils collected from a variety of sit... more Results of a preliminary study of keratinophilic fungi from soils collected from a variety of sites in hilly areas of Himachal Pradesh, India, are reported. A total of 122 soil samples were examined, of which 101 samples were positive for keratinophilic fungi and related dermatophytes. Eleven species of five genera were isolated viz. Chrysosporium indicum (11 %), C. keratinophilum (4 %), C. queenslandicum (25 %), C. tropicum (19 %), Chrysosporium sp. (2 %), C. xerophilum (2 %), Chrysosporium state of Ctenomyces serratus (2 %), Malbranchea gypsea (7 %), Microsporum canis (2 %), Microsporum gypseum complex (9 %) and Trichophyton mentagrophytes (1 %).

The Journal of Antibiotics, May 14, 2014

A novel depsipeptide (PM181110) was purified from an endophytic fungus Phomopsis glabrae isolated... more A novel depsipeptide (PM181110) was purified from an endophytic fungus Phomopsis glabrae isolated from the leaves of Pongamia pinnata (family Fabaceae). The chemical structure of PM181110 was elucidated using physiochemical properties, 2D NMR and other spectroscopic methods. PM181110 is very close in structure to FE399. The compound exhibited in vitro anticancer activity against 40 human cancer cell lines with a mean IC 50 value of 0.089 lM and ex vivo efficacy towards 24 human tumor xenografts (mean IC 50 ¼ 0.245 lM).