Nguyen Thanh Vu | Pukyong National University, Korea (original) (raw)

Several transgenic marine medaka Oryzias dancena strains harboring a green fluorescent protein (G... more Several transgenic marine medaka Oryzias dancena strains harboring a green fluorescent protein (GFP) reporter construct regulated by an endogenous β-actin promoter were established and their expression characteristics in relation to transgene copy numbers were examined in 21 transgene genotypes. Most of the transgenic strains displayed transgene insertion patterns typical of microinjection-mediated introduction of foreign DNA into fish embryos, characterized by the random integration of multiple transgene copies (ranging from 1 – 282 copies per cell), often accompanied by the formation of concatemer(s), as assessed by genomic Southern blot hybridization analysis and qPCR. Transgenic strains showed ubiquitous and continued temporal and spatial expression patterns of the transgenic GFP during most of their life cycle, from the embryonic stage to adulthood, enabling assessment of the expression pattern of the endogenous β-actin gene. However, a comparative evaluation of transgene copy numbers and expression levels showed that copy number-dependent expression, the stability of the ubiquitous distribution and expression efficiency per transgene copy varied among the transgenic strains. Fluorescence expression levels were positively correlated with absolute transgene copy numbers, whereas the expression efficiency per transgene copy was inversely related to the number of transgene integrant copies. Data from this study will guide the selection of potentially desirable transgenic strains with ubiquitous expression of a fluorescent transgene, not only in this marine medaka species but also in other related model fish species.

To evaluate their potential utility as an ornamental organism, novel transgenic marine medaka Ory... more To evaluate their potential utility as an ornamental organism, novel transgenic marine medaka Oryzias dancena strains with a highly vivid fluorescent phenotype were established through transgenesis of a cyan fluorescent protein gene (cfp) driven by the endogenous fast skeletal myosin light chain 2 gene (mlc2f) promoter. The transgenic marine medaka strains possessed multiple copies of transgene integrants and passed their fluorescent transgenes successfully to subsequent generations. Transgenic expression in skeletal muscles at both the mRNA and phenotypic levels was, overall, dependent upon transgene copy numbers. In the external phenotype, an authentic fluorescent color was dominant in the skeletal muscles of the transgenic fish and clearly visible to the unaided eye. The phenotypic fluorescent color presented differentially in response to different light-irradiation sources; the transgenics displayed a yellow-green color under normal daylight or white room light conditions, a strong green-glowing fluorescence under ultraviolet light, and a cyan-like fluorescence under blue light from a light-emitting diode.

Study on the maturated gonad and reproduction in pilot condition from pond culture is one of the ... more Study on the maturated gonad and reproduction in pilot condition from pond culture is one of the most important solutions to control source of broodstock Tiger shrimp, which is good quality of seed to support exported shrimp culture. Study the effects of various size and diets on reproduction of Tiger shrimp (Penaeus momodon), will feedback reproduction and quality of seed of F2 generation effectively. Broodstocks were colleted from pond of F1 – generation, and divided into three size groups of 110-120 g, 121-130 g, and 131-150 g. With 3 treatments of diet (T1,T2,T3): T1 100% fresh squid; T2 50% fresh squid: snail; T3 50% fresh squid: 25% snail: 25% worm, respectively. The results show that a positive correlation between size and fecundity of shrimp showing by formula ( = 0.82; = 0.74). The shrimp has 126.7 – 173.2 g in body weight and 23.3 – 25.5 cm in length, which has absolutely fecundity ca. 107200 – 253900 egg/individual. The requirement of nutrition in both treatments T2 and T3, which have been enough for their maturated culture and reproduction, it has absolutely fecundity of 250700 – 262600 egg/individual, respectively. The absolutely fecundity of T1 is lowest with 158600 egg/individual. In seed production, these results will promoting a program which has selected broodstock with the size has over of 130 g/individual and feeding not only single diet but also abundance source diet in the maturated cultured stage and reproduction.

Due to an increase in shrimp produce, natural exploitation has also increased leading to exhausti... more Due to an increase in shrimp produce, natural exploitation has also increased leading to exhaustion of the broodstock. Because of this, the source of broodstock in general and the males in practicular has been gradually rare and become more expensive. Moreover, in order to overcome the failure of natural intercourse. Title: study on Cryopreservation spermatophore of Penaeidea for artificial production may be able to solve the problem by actively producing artificial seeds. Content of implementation: study on morpholosis of sperm ability of sterilization of frozen sperm. The theis has been studied on two spesies: tiger shrimp Penaeus monodon and banana shrimp P. merguiensis. The sperm is preserved in Extender environment (EX1, EX2, EX3). Shrimp are wholly sryopreserved at 4 - 50C, and (-8)-(-10) 0C. The result has shown that at at 4 - 50C, the mean hatching rate is 19.39 – 42.12%. Preserving in EX1 IS 6.24 – 19.82%; in EX2 IS 0 – 11.67% and in EX3 is 0%. Wholly preserving the shrimp at (-8)-(-10) 0C is 0%, but in EX1 is 5.71 – 36.01%; EX2 is 2.28 – 23.95% and EX3 is 2.08 – 19.43%. the result has shown that the hatching rate in EX1 is the best. This theis should be soon carried out on other shrimp and preserving substances should also be experimented for moer appropriate method of preserving the spermatophore.

White leg shrimp (Litopenaeus vannamei) is imported to Vietnam from different countries (Hawaii, ... more White leg shrimp (Litopenaeus vannamei) is imported to Vietnam from different countries (Hawaii, China…) in 2001, which is serviced hatcheries and grow-out farming. The technologies that are relative to this species are not studied in Vietnam until 2001. Hence, to develop practicable white leg shrimp culture in Vietnam must to sciential and suitable studies, evaluation. Especially, study on seed production and grow-out in Vietnam would be effectively using of water ecosystem that is necessary and urgent requirements. The results of study on applied process of seed production and basis of science to service area culture scheme of white leg shrimp will be solutions above problems.

Đề tài được thực hiện nhằm mục đích đánh giá chất lượng tinh trùng tôm sú nuôi trong ao đìa và kh... more Đề tài được thực hiện nhằm mục đích đánh giá chất lượng tinh trùng tôm sú nuôi trong ao đìa và khả năng ứng dụng tôm F2 vào sản xuất giống; đồng thời so sánh chất lượng tinh trùng của tôm sú thành thục ngoài tự nhiên với tôm nuôi ao đìa để đưa ra những kết luận chính xác nhất. Đề tài gồm hai nội dụng chính: một là, đánh giá chất lượng tinh trùng của tôm sú thế hệ F2 nuôi trong ao đìa theo nhóm kích thước: 60-65 g, 66-70 g và >71 g; hai là, so sánh chất lượng tinh trùng của tôm sú đực thế hệ F2 nuôi trong ao đìa so với tôm sú đực thành thục ngoài tự nhiên. Kết quả cho thấy chất lượng tinh trùng của tôm sú nuôi trong ao đìa ở nhóm khối lượng từ 60 – 70 g là tốt nhất với tổng số tinh trùng đạt 155,9 – 205,4×106, tinh trùng bình thường đạt 80,9 – 81,7%, tinh trùng chết đạt 29,5 – 31,2% còn nhóm khối lượng > 71 g cho chất lượng tinh trùng kém hơn với tỷ lệ tinh trùng bình thường đạt 75,7%, tinh trùng chết đạt 37,2%. Đồng thời, sau khi tiến hành thí nghiệm cũng nhận thấy chất lượng tinh trùng của tôm thành thục ngoài tự nhiên tốt hơn tôm nuôi trong ao đìa, thể hiện ở chỗ số lượng tinh trùng giao động từ 280,0 - 358,8×106 , tỷ lệ phần trăm tinh trùng bình thường từ 84,3 - 87,5%, tinh trùng chết từ 14,6 - 17,9%.