Erythrocyte entry by malarial parasites. A moving junction between erythrocyte and parasite (original) (raw)

Abstract

Invasion of erythrocytes by merozoites of the monkey malaria, Plasmodium knowlesi, was investigated by electron microscopy. The apical end of the merozoite makes initial contact with the erythrocyte, creating a small depression in the erythrocyte membrane. The area of the erythrocyte membrane to which the merozoite is attached becomes thickened and forms a junction with the plasma membrane of the merozoite. As the merozoite enters the invagination in the erythrocyte surface, the junction, which is in the form of a circumferential zone of attachment between the erythrocyte and merozoite, moves along the confronted membranes to maintain its position at the orifice of the invagination. When entry is completed, the orifice closes behind the parasite in the fashion of an iris diaphragm, and the junction becomes a part of the parasitophorous vacuole. The movement of the junction during invasion is an important component of the mechanism by which the merozoite enters the erythrocyte. The extracellular merozoite is covered with a prominent surface coat. During invasion, this coat appears to be absent from the portion of the merozoite within the erythrocyte invagination, but the density of the surface coat outside the invagination (beyond the junction) is unaltered.

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Selected References

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  1. Aikawa M., Komata Y., Asai T., Midorikawa O. Transmission and scanning electron microscopy of host cell entry by Toxoplasma gondii. Am J Pathol. 1977 May;87(2):285–296. [PMC free article] [PubMed] [Google Scholar]
  2. Aikawa M. Parasitological review. Plasmodium: the fine structure of malarial parasites. Exp Parasitol. 1971 Oct;30(2):284–320. doi: 10.1016/0014-4894(71)90094-4. [DOI] [PubMed] [Google Scholar]
  3. Bannister L. H., Butcher G. A., Dennis E. D., Mitchell G. H. Structure and invasive behaviour of Plasmodium knowlesi merozoites in vitro. Parasitology. 1975 Dec;71(3):483–491. doi: 10.1017/s0031182000047247. [DOI] [PubMed] [Google Scholar]
  4. CHIN W., CONTACOS P. G., COATNEY G. R., KIMBALL H. R. A NATURALLY ACQUITED QUOTIDIAN-TYPE MALARIA IN MAN TRANSFERABLE TO MONKEYS. Science. 1965 Aug 20;149(3686):865–865. doi: 10.1126/science.149.3686.865. [DOI] [PubMed] [Google Scholar]
  5. Dennis E. D., Mitchell G. H., Butcher G. A., Cohen S. In vitro isolation of Plasmodium knowlesi merozoites using polycarbonate sieves. Parasitology. 1975 Dec;71(3):475–481. doi: 10.1017/s0031182000047235. [DOI] [PubMed] [Google Scholar]
  6. Dvorak J. A., Miller L. H., Whitehouse W. C., Shiroishi T. Invasion of erythrocytes by malaria merozoites. Science. 1975 Feb 28;187(4178):748–750. doi: 10.1126/science.803712. [DOI] [PubMed] [Google Scholar]
  7. Griffin F. M., Jr, Griffin J. A., Leider J. E., Silverstein S. C. Studies on the mechanism of phagocytosis. I. Requirements for circumferential attachment of particle-bound ligands to specific receptors on the macrophage plasma membrane. J Exp Med. 1975 Nov 1;142(5):1263–1282. doi: 10.1084/jem.142.5.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haywood A. M. 'Phagocytosis' of sendai virus by model membranes. J Gen Virol. 1975 Oct;29(1):63–68. doi: 10.1099/0022-1317-29-1-63. [DOI] [PubMed] [Google Scholar]
  9. Jensen J. B., Edgar S. A. Possible secretory function of the rhoptries of Eimeria magna during penetration of cultured cells. J Parasitol. 1976 Dec;62(6):988–992. [PubMed] [Google Scholar]
  10. Jensen J. B. Ultrastructure of the invasion of Eimeria magna sporozoites into cultured cells. J Protozool. 1975 Aug;22(3):411–415. doi: 10.1111/j.1550-7408.1975.tb05193.x. [DOI] [PubMed] [Google Scholar]
  11. Jones T. C., Yeh S., Hirsch J. G. The interaction between Toxoplasma gondii and mammalian cells. I. Mechanism of entry and intracellular fate of the parasite. J Exp Med. 1972 Nov 1;136(5):1157–1172. doi: 10.1084/jem.136.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ladda R., Aikawa M., Sprinz H. Penetration of erythrocytes by merozoites of mammalian and avian malarial parasites. J Parasitol. 1969 Jun;55(3):633–644. [PubMed] [Google Scholar]
  13. Miller L. H., Aikawa M., Dvorak J. A. Malaria (Plasmodium knowlesi) merozoites: immunity and the surface coat. J Immunol. 1975 Apr;114(4):1237–1242. [PubMed] [Google Scholar]
  14. Miller L. H., Mason S. J., Dvorak J. A., McGinniss M. H., Rothman I. K. Erythrocyte receptors for (Plasmodium knowlesi) malaria: Duffy blood group determinants. Science. 1975 Aug 15;189(4202):561–563. doi: 10.1126/science.1145213. [DOI] [PubMed] [Google Scholar]
  15. Rudzinska M. A., Trager W., Lewengrub S. J., Gubert E. An electron microscopic study of Babesia microti invading erythrocytes. Cell Tissue Res. 1976 Jun 28;169(3):323–334. doi: 10.1007/BF00219605. [DOI] [PubMed] [Google Scholar]
  16. Sheffield H. G. Electron microscope study of the proliferative form of Besnoitia jellisoni. J Parasitol. 1966 Jun;52(3):583–594. [PubMed] [Google Scholar]
  17. Singer S. J., Nicolson G. L. The fluid mosaic model of the structure of cell membranes. Science. 1972 Feb 18;175(4023):720–731. doi: 10.1126/science.175.4023.720. [DOI] [PubMed] [Google Scholar]