Survival of Arthrobacter crystallopoietes During Prolonged Periods of Extreme Desiccation (original) (raw)

Abstract

Cells of Arthrobacter crystallopoietes mixed in sand and air-dried have survived for up to 6 months after an initial period in which approximately half the cells lost their viability. Comparative survival curves have been obtained from inoculated sands maintained under CaSO4 or P2O5. Selections for more desiccation-resistant progeny capable of surviving the initial period were unsuccessful. Both the coccoid and rod-shaped forms are equally resistant to several months of desiccation. Desiccated spherical cells converted 0.0005% of their cell carbon to carbon dioxide per hr, which corresponds to a half-life for self-consumption of approximately 12 years.

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Selected References

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  1. Annear D. I., Bottomley G. A. Survival of bacteria in desiccates at 100 degrees C in dry atmospheres. Nature. 1965 Jun 26;206(991):1373–1374. doi: 10.1038/2061373a0. [DOI] [PubMed] [Google Scholar]
  2. Boylen C. W., Ensign J. C. Intracellular substrates for endogenous metabolism during long-term starvation of rod and spherical cells of Arthrobacter crystallopoietes. J Bacteriol. 1970 Sep;103(3):578–587. doi: 10.1128/jb.103.3.578-587.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Conn H. J., Dimmick I. Soil Bacteria Similar in Morphology to Mycobacterium and Corynebacterium. J Bacteriol. 1947 Sep;54(3):291–303. doi: 10.1128/jb.54.3.291-303.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Desser H., Broda E. Radiochemical determination of the endogenous and exogenous respiration of bacterial spores. Nature. 1965 Jun 19;206(990):1270–1271. doi: 10.1038/2061270b0. [DOI] [PubMed] [Google Scholar]
  5. Desser H., Broda E. Radiochemical investigation of the respiration of spores of Bacillus cereus. Arch Mikrobiol. 1969;65(1):76–86. doi: 10.1007/BF00412066. [DOI] [PubMed] [Google Scholar]
  6. ENSIGN J. C., WOLFE R. S. NUTRITIONAL CONTROL OF MORPHOGENESIS IN ARTHROBACTER CRYSTALLOPIETES. J Bacteriol. 1964 Apr;87:924–932. doi: 10.1128/jb.87.4.924-932.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ensign J. C. Long-term starvation survival of rod and spherical cells of Arthrobacter crystallopoietes. J Bacteriol. 1970 Sep;103(3):569–577. doi: 10.1128/jb.103.3.569-577.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Robinson J. B., Salonius P. O., Chase F. E. A note on the differential response of arthrobacter spp. and pseudomonas spp. to drying in soil. Can J Microbiol. 1965 Aug;11(4):746–748. doi: 10.1139/m65-100. [DOI] [PubMed] [Google Scholar]
  9. SNEATH P. H. Longevity of micro-organisms. Nature. 1962 Aug 18;195:643–646. doi: 10.1038/195643a0. [DOI] [PubMed] [Google Scholar]
  10. WOELLER F. H. Liquid scintillation counting of C-14-labelled CO2 with phenethylamine. Anal Biochem. 1961 Oct;2:508–511. doi: 10.1016/0003-2697(61)90056-2. [DOI] [PubMed] [Google Scholar]