GABAA receptors are differentially sensitive to zinc: dependence on subunit composition (original) (raw)

Abstract

GABAA receptors with different subunit composition, were expressed in kidney cells and studied by whole cell recording. Expressed GABAA receptors were differentially sensitive to inhibition by zinc; receptors which lacked the gamma subunit were inhibited by zinc. Embryonic neurons also exhibited zinc-sensitive GABA responses, in contrast to adult neurones. This developmentally-sensitive aspect of GABAA receptor pharmacology may be partly dependent on expression of the gamma subunit.

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Selected References

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  1. Draguhn A., Verdorn T. A., Ewert M., Seeburg P. H., Sakmann B. Functional and molecular distinction between recombinant rat GABAA receptor subtypes by Zn2+. Neuron. 1990 Dec;5(6):781–788. doi: 10.1016/0896-6273(90)90337-f. [DOI] [PubMed] [Google Scholar]
  2. Kofuji P., Wang J. B., Moss S. J., Huganir R. L., Burt D. R. Generation of two forms of the gamma-aminobutyric acidA receptor gamma 2-subunit in mice by alternative splicing. J Neurochem. 1991 Feb;56(2):713–715. doi: 10.1111/j.1471-4159.1991.tb08209.x. [DOI] [PubMed] [Google Scholar]
  3. Levitan E. S., Blair L. A., Dionne V. E., Barnard E. A. Biophysical and pharmacological properties of cloned GABAA receptor subunits expressed in Xenopus oocytes. Neuron. 1988 Nov;1(9):773–781. doi: 10.1016/0896-6273(88)90125-0. [DOI] [PubMed] [Google Scholar]
  4. Malherbe P., Draguhn A., Multhaup G., Beyreuther K., Möhler H. GABAA-receptor expressed from rat brain alpha- and beta-subunit cDNAs displays potentiation by benzodiazepine receptor ligands. Brain Res Mol Brain Res. 1990 Aug;8(3):199–208. doi: 10.1016/0169-328x(90)90017-8. [DOI] [PubMed] [Google Scholar]
  5. Moss S. J., Smart T. G., Porter N. M., Nayeem N., Devine J., Stephenson F. A., Macdonald R. L., Barnard E. A. Cloned GABA receptors are maintained in a stable cell line: allosteric and channel properties. Eur J Pharmacol. 1990 Jul 31;189(1):77–88. doi: 10.1016/0922-4106(90)90232-m. [DOI] [PubMed] [Google Scholar]
  6. Pritchett D. B., Sontheimer H., Shivers B. D., Ymer S., Kettenmann H., Schofield P. R., Seeburg P. H. Importance of a novel GABAA receptor subunit for benzodiazepine pharmacology. Nature. 1989 Apr 13;338(6216):582–585. doi: 10.1038/338582a0. [DOI] [PubMed] [Google Scholar]
  7. Smart T. G., Constanti A. Differential effect of zinc on the vertebrate GABAA-receptor complex. Br J Pharmacol. 1990 Apr;99(4):643–654. doi: 10.1111/j.1476-5381.1990.tb12984.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Smart T. G. Uncultured lobster muscle, cultured neurons and brain slices: the neurophysiology of zinc. J Pharm Pharmacol. 1990 Jun;42(6):377–387. doi: 10.1111/j.2042-7158.1990.tb06576.x. [DOI] [PubMed] [Google Scholar]
  9. Verdoorn T. A., Draguhn A., Ymer S., Seeburg P. H., Sakmann B. Functional properties of recombinant rat GABAA receptors depend upon subunit composition. Neuron. 1990 Jun;4(6):919–928. doi: 10.1016/0896-6273(90)90145-6. [DOI] [PubMed] [Google Scholar]
  10. Westbrook G. L., Mayer M. L. Micromolar concentrations of Zn2+ antagonize NMDA and GABA responses of hippocampal neurons. Nature. 1987 Aug 13;328(6131):640–643. doi: 10.1038/328640a0. [DOI] [PubMed] [Google Scholar]