Bacteria-Free Solution Derived from Lactobacillus plantarum Inhibits Multiple NF-KappaB Pathways and Inhibits Proteasome Function (original) (raw)

. Author manuscript; available in PMC: 2009 Oct 1.

Published in final edited form as: Inflamm Bowel Dis. 2009 Oct;15(10):1537–1547. doi: 10.1002/ibd.20930

Abstract

Background

Bacteria play a role in inflammatory bowel disease and other forms of intestinal inflammation. Although much attention has focused on the search for a pathogen or inciting inflammatory bacteria, another possibility is a lack of beneficial bacteria that normally confer anti-inflammatory properties in the gut. The purpose of this study was to determine whether normal commensal bacteria could inhibit inflammatory pathways important in intestinal inflammation.

Methods

Conditioned media from Lactobacillus plantarum (Lp-CM) and other gut bacteria was used to treat intestinal epithelial cell (YAMC) and macrophage (RAW 264.7) or primary culture murine dendritic cells. NF-_κ_B was activated through TNF-Receptor, MyD88-dependent and -independent pathways and effects of Lp-CM on the NF-_κ_B pathway were assessed. NF-_κ_B binding activity was measured using ELISA and EMSA. 1_κ_B expression was assessed by Western blot analysis, and proteasome activity determined using fluorescence-based proteasome assays. MCP-1 release was determined by ELISA.

Results

Lp-CM inhibited NF-κ_B binding activity, degradation of I_κ_B_α and the chymotrypsin-like activity of the proteasome. Moreover, Lp-CM directly inhibited the activity of purified mouse proteasomes. This effect was specific, since conditioned media from other bacteria had no inhibitory effect. Unlike other proteasome inhibitors, Lp-CM was not toxic in cell death assays. Lp-CM inhibited MCP-1 release in all cell types tested.

Conclusions

These studies confirm, and provide a mechanism for, the anti-inflammatory effects of the probiotic and commensal bacterium Lactobacillus plantarum. The use of bacteria-free Lp-CM provides a novel strategy for treatment of intestinal inflammation which would eliminate the risk of bacteremia reported with conventional probiotics.

Keywords: NF-kappaB, probiotics, inflammatory bowel diseases, intestinal microbiota, proteasome

Changes in gut flora have been implicated in the etiologies of inflammatory bowel disease (IBD), anti- biotic-associated diarrhea, and even obesity.1–4 While an inciting bacteria or pathogen may be causal, equally plausible is the possibility that the relative absence of commensal bacteria, normally serving a protective or anti-inflammatory role, may exacerbate intestinal inflammation. Indeed, the lack of certain classes of bacteria has already been associated with human intestinal diseases. In irritable bowel syndrome (IBS), 16S rDNA studies showed a complete absence of certain Lactobacillus species in affected individuals.5 Similarly, human intestinal biopsies have demonstrated a lower proportion of Bifidobacteria in patients with IBD compared to healthy controls.6

Consequently, the use of probiotics to enhance or modify the intestinal bacteria profile has generated much interest.7 Probiotics are defined as ingested microorganisms that provide health benefits beyond their intrinsic nutritional value. The majority of probiotics are derived from bacteria that naturally colonize the human intestine.8,9 However, although commensal and probiotic bacteria may confer beneficial effects on the host, the average “recommended dose” of probiotic consists of billions of live bacteria. Several reports in the literature have raised safety concerns over the practice of ingesting such large bacterial loads, especially in sick and immunocompromised patients.10–14 Recent clinical trials of acute pancreatitis, prematurely terminated due to increased mortality in the probiotic group, further prompt caution in using live probiotics to treat disease.15

Although some protective effects of probiotics require direct bacterial-epithelial cell-to-cell contact with live bacteria, we previously demonstrated that VSL#3, a probiotic mixture used to treat pouchitis,16 produces bioactive factors that inhibit NF-_κ_B.17 The use of bacteria-free products synthesized by bacteria, rather than the live bacteria themselves, may be an approach to improve safety by eliminating the risk of infection reported with many conventional probiotic therapies.18–20

The purpose of this study was two-fold: first, to determine whether single strains of commensal bacteria, which commonly colonize the GI tract, secrete bioactive factors with anti-inflammatory properties. Second, if found, to define the mechanism by which these bioactive factors exert their anti-inflammatory effects. Here we report that a commensal bacteria, Lactobacillus plantarum, produces bioactive factors (conditioned media) that inhibit chymotrypsin-like proteasome function and thus broadly influence the inflammatory activation of NF-_κ_B pathways due to multiple stimuli and in various cell lines. This effect was specific to L. plantarum, as the other bacteria tested did not elicit these effects. The use of bacteria-free _L. plantarum_-conditioned media (Lp-CM) provides a novel strategy for treatment of intestinal inflammation.

MATERIALS AND METHODS

Preparation of Bacteria-Conditioned Media

The Escherichia coli (F18 strain), a normal commensal strain of E. coli and a generous gift of Dr. Beth McCormick, and EPEC (enteropathogenic E. coli, gift of Dr. Gail Hecht) were grown using Luria-Bertani (LB) agar plates and LB broth (Difco, Detroit, MI). Bacteria were grown overnight at 37°C under agitating conditions. Salmonella enterica serovar typhimurium American Type Culture Collection (ATCC, Manassas, VA, strain 14028) was grown in LB as above, but under microaerophilic, nonagitating conditions as previously described.21 The Bacteroides fragilis strain (ATCC No. 25285) and the Bifidobacterium breve (ATCC No. 15700) were grown overnight in chopped meat broth or on blood agar plates (BD Diagnostics, Sparks, MD) under GasPak (Mitsubishi Gas Chemical, New York, NY) anaerobic conditions. All bacterial suspensions were grown to the same Optical Density (as measured at 600 nm) prior to harvest. Conditioned media was prepared by aseptic filtration of the suspension culture through 0.22 _μ_m low protein binding cellulose acetate filters (Millipore, Billerica, MA).

The L. plantarum and L. paracasei strains used in this study were the generous gift of Dr. Stig Bengmark. L. plantarum, L. paracasei and L. acidophilus (ATCC No. 53544) were grown first in MRS (DeMan Rogosa & Sharpe, Difco) broth at 37°C, 5% CO2 under non-agigating conditions, centrifuged (20 min, 5400 rpm), and resuspended in modified Hank’s balanced saline solution (HBSS; Invitrogen, Carlsbad, CA) supplemented with 0.04 M MgSO4, 0.03 M MnSO4, 1.15 M K2PO4, 0.36 M sodium acetate, 0.88 M ammonium citrate, 10% polysorbate (growth factor for Lactobacillus sp) and 20% dextrose, then propagated overnight at 37°C, 5% CO2 nonagitating conditions (2 × 109 cfu/mL). The culture was again centrifuged and the supernatant (conditioned media) aseptically filtered using 0.22 _μ_m low protein binding cellulose acetate filters (Millipore).

Cell Culture

Murine RAW 264.7 macrophage cells (ATCC No. TIB-71) were maintained at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) with 4 mM L-glutamine and 10% fetal bovine serum and used up to passage 30.

YAMC (young adult mouse colon) cells (gift of Dr. R. Whitehead, Vanderbilt University, Nashville, TN)22 were maintained under permissive conditions (33°C) in RPMI 1640 medium with 5% (vol/vol) fetal bovine serum, 5U/mL murine IFN-γ (Gibco BRL, Grand Island, NY), 50 _μ_g/mL streptomycin, 50 U/mL penicillin, supplemented with ITS+ Premix (BD Biosciences, Bedford, MA), and differentiated under nonpermissive conditions at 37°C as previously described.17 Cells were pretreated with Lp-CM for 4 hours, then treated with either TNF-α, 30 ng/mL for 30 minutes (Peprotech, Rocky Hill, NJ), flagellin, 100 ng/mL for 30 minutes (Axxora LCC, San Diego, CA), or poly dI:dC, 50 _μ_g/mL for 16 hours (Invivogen, San Diego, CA).

Preparation of Primary Murine Dendritic Cells

Dendritic cells were isolated from the hindlimbs of 6-week-old C57/Bl6 female mice using the method of Inaba et al.23 Briefly, bone marrow was collected by passing HBSS through the hindlimb, followed by centrifugation and addition of 5 mL sterile Tris-NH4Cl buffer. RPMI complete medium (10% FCS, 1× penicillin-streptomycin-glutamine, 250 _μ_g/ml gentamicin) was then added and cells centrifuged again. Cells were resuspended in complete RPMI supplemented with 2.5 ng/mL GM-CSF (eBiosciences, San Diego, CA) and 5 ng/mL IL-4 (R&D Systems, Minneapolis, MN) and plated on 6-well plates. On day 7–9, cells were collected, pelleted, resuspended in complete RPMI, and plated on 24-well plates. After 4 hours, cells were treated with conditioned medium for 3 hours followed by treatment with lipopolysaccharide (LPS) overnight, then supernatant collected for MCP-1 enzyme-linked immunosorbent assay (ELISA).

Preparation of Nuclear Extracts

Nuclear extracts were harvested utilizing a method modified from Inan et al.24 Briefly, cells were washed and harvested in phosphate-buffered saline, then incubated 15 minutes in extract lysis buffer (10 mM Hepes, 10 mM KCl, 0.1 mM EDTA, 2 mM MgCl2, 0.5 mM sucrose, 0.05% Nonidet P-40 (NP40), 0.5 mM PMSF, 1 mM DTT, 1× complete protease inhibitor). Nucleus-containing pellets were rinsed with extract lysis buffer without NP40 and incubated 40 minutes in high salt buffer (20 mM Hepes pH 7.4, 1.5 mM MgCL2, 420 mM NaCl, 0.2 mM EDTA, 5% glycerol, 1 mM DTT, 0.5 mM PMSF, 1× complete protease inhibitor; Roche Molecular Biochemicals, Indianapolis, IN). The nuclear extract was removed and combined with low salt buffer (20 mM Hepes, pH 7.4, 50 mM NaCl, 0.2 mM EDTA, 20% glycerol, 1 mM DTT, 0.5 mM PMSF, 1× complete protease inhibitor). Protein concentrations were determined by Bio-Rad protein assay (Hercules, CA).

NF-_κ_B electrophoretic mobility shift assay (EMSA): At room temperature, nuclear extracts (5–10 _μ_g) were incubated with 5 mM Tris, pH 7.5, 0.5 mM EDTA, 2% Ficoll, 0.5 mM DTT, 37.5 mM KCl, 1 _μ_g poly dI:dC (Roche), and 50,000 cpm of a _γ_-32P-labeled probe encoding the NF-_κ_B consensus sequence (5′-AGTTGAGGGGACTTTCC CAGGC-3′) (Promega, Madison, WI). Samples were electrophoresed on a native 5% polyacrylamide gel. The gels were then dried and exposed to film. To determine oligonucleotide specificity, a 100-fold excess cold oligonucleotide was added to the reaction mixture prior to incubation with probe (data not shown).

MCP-1 ELISA

YAMC and RAW 264.7 cells were pretreated with Lp-CM for 4 hours and subsequently treated with TNF-α 30 ng/mL to stimulate NF-_κ_B activation. Dendritic cells were pretreated with Lp-CM or vehicle control HBSS for 3 hours and then treated overnight with LPS (1 _μ_g/mL). Supernatants were harvested and tested for the production of MCP-1 (monocyte chemotactic protein-1) using a mouse MCP-1 ELISA kit (Pierce Endogen, Rockford, IL) as per the manufacturer’s instructions.

Cell Death Assay

Sample preparation and cell death ELISA assay was performed according to the manufacturer’s instructions (Roche Applied Science).

IκB Western Blot Analysis

Twenty micrograms of protein per lane was resolved on 11% SDS-PAGE and transferred using a semi-dry transfer system (Bio-Rad) in transfer buffer (200 mL MeOH, 3.03 g Tris, and 14.4 g glycine/L) onto 0.45 _μ_m nitrocellulose membranes (Millipore).25 Membranes were blocked in 5% (wt/vol) nonfat milk in TBS-Tween (Tris-buffered saline, 150 mM NaCl, 5 mM KCl, 10 mM Tris, pH 7.4, with 0.05% (vol/vol) Tween 20) prior to incubation with primary Anti-I_κ_B-α antibody (sc-1643, Santa Cruz Bio-technology, Santa Cruz, CA) and a peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, Fort Washington, PA). Blots were exposed to autoradiographic film and then visualized using an enhanced chemiluminescence (ECL) reagent (Supersignal, Pierce).

Proteasome Assay

Cells were harvested as previously described.17 Proteasome activity was measured using a proteasome assay kit (Calbiochem, San Diego, CA) with 20 _μ_g of each sample added to proteasome assay reaction buffer (25 mM HEPES, 0.5 mM EDTA, pH 7.6, 0.03% (wt/vol) SDS), then 10 _μ_M of the substrate suc-leu-leu-val-tyr-AMC (SLLVY-AMC) was added. Proteasome chymotrypsin-like activity was determined by measuring the fluorogenic signal generated by cleavage and release of the fluorogenic compound AMC (7-amino-4-methylcoumarin) over time. Fluorescence (excitation 380 nm, emission 460 nm) was measured with a Synergy-2 fluorometer (Bio-Tek Instruments, Winooski, VT) and proteasome activity was determined by calculating the rate (slope) of AMC production over time using KC4 software (Bio-Tek Instruments). Cells were treated with MG132 as a positive inhibitor control at a concentration of 25 _μ_M and untreated cells with DMSO as a vehicle control for MG132.

Proteasome assays from purified liver preparations were performed as described above except that a 96-well plate reader (either Biotek Synergy-2, or Varian Eclipse) was used to screen fractions, and in addition to MG132, epoxomicin (Calbiochem:EMD Chemicals, Gibbstown, NJ), a highly specific proteasome inhibitor,26 was used at 5 _μ_M, as a positive inhibitor control to ensure that preparations contained pure proteasome activity. Fluorescence measurements were taken every minute and the slope (proteasome activity) was calculated using KC4 software (for Bio-Tek plate-reader) or Cary-Eclipse software (for Varian plate-reader). Concentrations of the SLLVY-AMC substrate were varied from 25 _μ_M to 100 _μ_M, along with varying concentrations of Lp-CM, in order to perform the Dixon plot to determine the type of inhibition. For the Dixon plot analysis the slope of the reaction was used within the first 5 minutes, to calculate the initial reaction rate, or Vi, with readings being taken every minute for 30 minutes thereafter. These data were then analyzed and Dixon plot generated using Grafit software v. 6 (Erithacus, West Sussex, UK).

Isolation and Purication of Proteasome (20S)

Purified proteasomes were prepared based on the method described by Hirano et al,27 with several modifications as follows: Mice were male 8-week-old CD-1 mice obtained from Charles River Laboratories. Each excised mouse liver was homogenized in 2 mL ice-cold homogenizing buffer (25 mM Tris-HCl, pH 7.5, 0.25 M sucrose, 1 mM DTT) then centrifuged at 70,000_g_ for 1 hour at 4°C (Sorvall Ultra Pro 80 centrifuge with T-865). Glycerol was added to a final concentration of 10%, then the supernatant was mixed with ≈20 mL of Q-Sepharose (Amersham Bioscience, Piscataway, NJ) equilibrated with Buffer A (25 mM Tris-HCl, pH 7.5, containing 10% glycerol and 1 mM DTT) for 30 minutes, then gently centrifuged at 3000 rpm for 10 minutes (Sorvall RC 5C centrifuge with SS-34 rotor). The supernatant was discarded, Q-Sepharose was suspended in Buffer A, and packed into a glass chromatography column (2 × 15 cm, Pharmacia, Gaithersburg, MD). Proteins were eluted by a linear gradient of NaCl from 0–0.8 M using 120 mL each of Buffers A and B (Buffer A plus 0.8 M NaCl), fractions of 1.5 mL each were collected using a fraction collector (Gilson, Worthington, OH). The eluted protein fractions were measured at 280 nm (Beckman-Coulter, Fullerton, CA; DU 530) and salt concentration was monitored using a conductivity meter (Radiometer CDM 210). Eluted peaks were tested for proteasomal activity using the substrate suc-LLVY-AMC 65 μ_M as described above. Excess salt was removed using dialysis tubing, active fractions were combined, and polyethylene-glycol was added to a final concentration of 15% while stirring gently. After 15 minutes the mixture was centrifuged at 10,000_g for 20 minutes, the supernatant was discarded, and the precipitate dissolved into Buffer A. The proteasomal activity of separated fractions was confirmed using the substrate SLLVY-AMC and the highly specific proteasome inhibitor epoxomicin (Calbiochem).26 The final protein concentration was determined using absorbance at 280 nm. BSA was used as a calibration standard.

Physical Characterization of Lp-CM Bioactive Factors

Lp-CM was tested for heat stability by boiling for 10 minutes and for protease sensitivity by pepsin digestion, as previously described, prior to testing for bioactivity in proteasome assays.28

Statistical Analysis

Data were analyzed by analysis of variance (ANOVA) with Bonferroni correction, and P < 0.05 was considered statistically significant. Statistical analysis was performed using GraphPad Instat software (GraphPad Software, San Diego CA). Graphs represent mean ± standard deviation for 3 separate experiments, and in each experiment each treatment group was performed in triplicate unless otherwise specified. Experiments were repeated a minimum of 3–6 times each. For Figure 6, vehicle and Lp-CM treated sample pairs were compared using Student’s _t_-test (P < 0.05 was accepted as a level of statistically significant difference).

FIGURE 6.

FIGURE 6

Pretreatment with Lp-CM inhibits NF-_κ_B activation by multiple signaling pathways. Lp-CM was used to treat YAMC cells, an intestinal epithelial cell line derived from mouse large intestine (A) or RAW 264.7 cells, a murine macrophage cell line (B). Cells were activated either with 100 ng/mL flagellin (TLR5 ligand, MyD88-dependent), or with poly dI:dC (TLR3 ligand, MyD88-independent) and NF-_κ_B activity was tested using an NF-_κ_B ELISA assay (Active Motif) as described in Materials and Methods. TNF-α activation is also shown. Data are presented as mean ± SD for 3 separate experiments and vehicle (gray bars) versus Lp-CM treated sample (black bars) pairs were compared using Student’s _t_-test (P < 0.05 was accepted as a level of statistically significant difference).

RESULTS

Assessment of Intestinal Bacteria-Conditioned Media Inhibition of NF-κB Binding in Gut Epithelial Cells

To assess the ability of soluble factors from different intestinal bacteria to inhibit NF-_κ_B, intestinal epithelial cells (YAMC cells) were pretreated with conditioned media from several different Gram-positive and Gram-negative commensal bacteria, and then treated with TNF-α to stimulate NF-_κ_B. Nuclear extracts were harvested and analyzed for binding activity of NF-_κ_B using a commercially available ELISA assay (Active Motif). As shown in Figure 1, only Lp-CM inhibited NF-_κ_B.

FIGURE 1.

FIGURE 1

Assessment of intestinal bacteria conditioned media to inhibit NF-_κ_B binding in gut epithelial cells. YAMC cells were either pretreated with conditioned media alone from the intestinal bacteria indicated (gray bars), or pre-treated and then stimulated with 30 ng/mL TNF-α for 30 minutes (black bars). A commercially available NF-_κ_B ELISA (Active Motif) was then used to test nuclear extracts and determine degree of NF-_κ_B binding. Results are expressed as mean ± SD for a minimum of 3 separate experiments (*P < 0.01 by ANOVA analysis using Bonferroni correction).

Lactobacillus plantarum CM Inhibits NF-κB Binding in Intestinal Epithelial Cells (IEC)

To further define the nature of the NF-_κ_B inhibition, YAMC cells were pretreated with Lp-CM for the times indicated, treated with TNF-α to stimulate NF-_κ_B, then cell nuclear extracts were harvested for analysis by EMSA to directly examine binding of NF-_κ_B to its target DNA consensus sequence (Fig. 2). Results for Lp-CM were compared to the proteasome inhibitor MG132, which inhibits basal and TNF-α-stimulated NF-_κ_B activity. It can be seen that Lp-CM inhibits binding of the p50/p65 isoform of NF-_κ_B. This observation was confirmed by NF-_κ_B ELISA, which was performed with a longer time course, up to 6 hours (data not shown).

FIGURE 2.

FIGURE 2

Lp-CM inhibits NF-_κ_B binding in intestinal epithelial cells. YAMC cells were pretreated with either MG132 or Lp-CM for the times indicated (Time Tx), and then treated with 30 ng/mL TNF-α for the times indicated prior to nuclear extract harvest and electrophoretic mobility shift assay (EMSA). Pretreatment with Lp-CM blocks TNF-α-induced NF-_κ_B binding. Nonspecific binding (NS) indicated, free probe is at bottom of gel. Blot shown is representative of 3 independent experiments.

Using antibodies against the other NF-_κ_B subunits in supershift assays, it was confirmed that the p50/p65 subunit is the major NF-_κ_B isoform inhibited by Lp-CM (data not shown).

Lp-CM Inhibits Release of MCP-1 from Multiple Cell Types

In order to establish whether Lp-CM inhibits target genes important in the intestinal inflammatory response, we measured release of MCP-1 (monocyte chemotactic protein-1), an inflammatory chemokine important in leukocyte recruitment to areas of inflammation and a well-described downstream gene target of NF-_κ_B.29,30 YAMC cells were pretreated with Lp-CM and subsequently treated with TNF-α to activate NF-_κ_B. Supernatants were harvested and tested for the production of MCP-1 by ELISA (Fig. 3A). Lp-CM attenuated the release of MCP-1 in response to TNF-α compared to both untreated control and L. paracasei and _E. coli_-CM treated controls. Since macrophages play an important role in mucosal immunity, the murine macrophage cell line RAW 264.7 was similarly tested and also demonstrated Lp-CM blockade of TNF-α-induced MCP-1 release (Fig. 3B). Finally, Lp-CM decreased MCP-1 release from primary murine dendritic cells in response to LPS stimulation (Fig. 3C). Collectively, these data demonstrate that intestinal epithelial cells, white blood cells, and dendritic cells all exhibit a dampening of the inflammatory response upon exposure to Lp-CM, providing supporting evidence that Lp-CM exerts its effects across multiple cell types important in the intestinal inflammatory response.

FIGURE 3.

FIGURE 3

Lp-CM inhibits TNF-α-mediated MCP-1 release in multiple cell types. A: YAMC cells were treated with either Lp-CM, _L. paracasei_-CM, or _E. coli_-CM for 4 hours, and then with TNF-α (30 ng/mL) to stimulate NF-_κ_B. Supernatants were harvested after 6 hours and and then assayed for release of the chemokine MCP-1, a downstream gene target of NF-_κ_B, using a commercially available ELISA. Results were compared to vehicle-treated control (No Treatment, column 1), or TNF-α treatment alone (column 2). Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA analysis using Bonferroni correction). B: RAW 264.7 murine macrophage cells were treated with either Lp-CM, _L. paracasei_-CM, or _E. coli_-CM for 4 hours, then with TNF-α (30 ng/mL) to activate NF-_κ_B. Supernatants were harvested after 6 hours and and then assayed for release of MCP-1 using a commercially available ELISA. Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA analysis using Bonferroni correction). C: Primary murine dendritic cells were prepared as described in Materials and Methods and then treated with either Lp-CM, _L. paracasei_-CM, or _E. coli_-CM for 3 hours, followed by LPS treatment for 16 hours to activate NF-_κ_B. Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA analysis using Bonferroni correction).

Lp-CM Inhibits Proteasome Activity in Different Cell Types

We and others have shown that the probiotic mixture VSL#3 inhibits proteasome function in IEC.17,31,32 To determine whether Lp-CM affects proteasome function, CTL-like proteasome activity was measured in extracts from YAMC cells (Fig. 4A) and RAW macrophages (Fig. 4B) after treatment with Lp-CM, _E. coli_-CM, _L. paracase_i-CM, or the synthetic proteasome inhibitor MG132. Only Lp-CM inhibited the CTL-like activity of the proteasome.

FIGURE 4.

FIGURE 4

Lp-CM inhibits proteasome activity in different cell types. A: YAMC cells were treated with Lp-CM, _L. paracasei_-CM, or _E. coli_-CM or the synthetic proteasome inhibitor MG132 for 1 hour, then harvested for proteasome assay using SLLVY-AMC substrate (see text). Results are expressed as proteasome activity in fluorescence units/min over time. Activity was determined by measuring fluorescence (excitation 380 nm, emission 460 nm) every 3 minutes for 30 minutes to determine reaction rate. Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA with Bonferroni correction). B: RAW 264.7 macrophage cells were treated with Lp-CM, _L. paracasei_-CM, or _E. coli_-CM or the synthetic proteasome inhibitor MG132 for 1 hour, then harvested for proteasome assay using SLLVY-AMC substrate as in panel A. Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA with Bonferroni correction).

Lp-CM Preserves Proteins Normally Degraded by the Proteasome

The NF-_κ_B inhibitor, I_κ_Bα, normally sequesters NF-_κ_B in the cytoplasm in an inactive state but can be degraded by the CTL-like activity of the proteasome, allowing release and activation of NF-κ_B. Hence, to determine whether proteasome inhibition by Lp-CM resulted in blocked degradation of I_κ_Bα, YAMC cells were either pre-treated with Lp-CM or HBSS, then stimulated with TNF-α and harvested over a 90-minute time course for Western blot analysis of I_κ_Bα. Compared to HBSS controls, Lp-CM pretreatment inhibited degradation of I_κ_B_α (Fig. 5), consistent with the Lp-CM inhibition of proteasome activity shown in Figure 4.

FIGURE 5.

FIGURE 5

I_κ_B_α_ degradation is inhibited by Lp-CM. YAMC cells were pretreated with either vehicle control (HBSS) or with Lp-CM for 1 hour, then stimulated with TNF-α (30 ng/mL) and harvested at the timepoints (min) indicated. Samples were then subjected to Western blot analysis for the presence of I_κ_Bα. Hsc73 (heat shock cognate 73) was used as a loading control. Blot shown is representative of 3 separate experiments.

Lp-CM Inhibits TLR Signaling Pathways

In addition to stimulation by TNF-α, NF-_κ_B can be activated by Toll-like receptor (TLR) pathways. TLRs recognize various bacterial and viral components and play a key role in innate immunity. If inhibition of NF-_κ_B occurs through a mechanism of proteasome blockade, we reasoned that Lp-CM should inhibit the effect of multiple NF-_κ_B-activating ligands which are located upstream of the proteasome. Hence, the effect of Lp-CM on MyD-88-dependent and MyD-88-independent TLR pathways was investigated by NF-_κ_B ELISA. Following Lp-CM pretreatment the protein flagellin (ligand for TLR5), which is dependent on signaling through the adaptor molecule MyD88, and the nucleotide poly dI:dC (ligand for TLR3), which acts through a MyD88-independent pathway, were used to activate NF-_κ_B. Lp-CM inhibited NF-_κ_B binding in response to both MyD88-dependent and MyD88-independent ligands in both a macrophage cell line and an IEC line (Fig. 6A, B). Conditioned media from L. paracasei failed to inhibit NF-_κ_B (data not shown).

Lp-CM Directly Inhibits Proteasome Activity

The data from Figures 4, 5, and 6 support the conclusion that Lp-CM inhibits NF-_κ_B through inhibition of proteasome function, but do not provide any indication as to whether the inhibition occurs indirectly, mediated through signal transduction pathways, or whether this occurs as a result of a direct interaction with the proteasome itself. To determine whether Lp-CM was acting directly on the proteasome, mouse liver proteasomes were isolated and proteasome activity measured after treatment with Lp-CM, or conditioned media from L. paracasei or E. coli. The proteasome inhibitors epoxomicin, MG132, and lactic acid were included as additional controls. Lp-CM specifically directly inhibits the chymotrypsin-like activity in purified proteasome preparations from mouse liver (Fig. 7A). In addition, based on Dixon plot analysis, Lp-CM exhibits a dose-dependent inhibitory effect on the proteasome, and is a noncompetitive, reversible proteasome inhibitor (Fig. 7B). Preliminary characterization of the proteasome-inhibiting bioactive factors in Lp-CM indicate that they are of small molecular weight (less than 10 kDa, data not shown), heat stable (Fig. 8A), and resistant to pepsin digestion (Fig. 8B), indicating they are not bacterial proteins.

FIGURE 7.

FIGURE 7

Lp-CM directly inhibits proteasome activity and is a reversible proteasome inhibitor. A: Proteasomes were purified from mouse liver as described in Materials and Methods, treated with Lp-CM, _L. paracasei_-CM, or _E. coli_-CM, and then proteasome assays were performed immediately to determine whether the effect of Lp-CM was a direct inhibitory effect on the proteasome. A fluorometer was used to record measurements every minute for 30 minutes (excitation 380 nm, emission 460 nm) and slopes were calculated using CaryEclipse and KC4 software (see Materials and Methods). Results are expressed as percent activity, with untreated proteasome preparations defined as 100% activity (y-axis). Epoxomicin, a highly specific proteasome inhibitor, was used as an inhibitor control to ensure absence of other contaminating proteases. MG132 is also shown. Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA with Bonferroni correction). B: Proteasome assays were performed using varying concentrations of SLLVY-AMC substrate and varying concentrations of Lp-CM as indicated. Reaction rates were used within the first 5 minutes of starting the assay in order to determine Vi (initial reaction rate) for generation of the Dixon plot data. Dixon plots were generated using Grafit software.

FIGURE 8.

FIGURE 8

Bioactive factors in Lp-CM are heat-stable and protease-resistant. A: Purified proteasomes from mouse liver were treated with Lp-CM or L. paracasei-CM that was thoroughly boiled for 10 minutes. Treatments were added directly to the proteasome preparation, SLLVY-AMC substrate was added, and the samples assayed for proteasome activity as previously described (excitation 380 nm, emission 460 nm). Slopes were calculated using CaryEclipse software (see Materials and Methods) and results expressed as percent activity, with untreated proteasome preparations defined as 100% activity (y-axis). MG132 is also shown. Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA with Bonferroni correction). B: Proteasome assays were performed as in panel A except that Lp-CM and _L. paracasei_-CM were subjected to protease digestion using a pepsin digestion method as previously described.28 Results are expressed as mean ± SD for 3 separate experiments (*P < 0.01 by ANOVA with Bonferroni correction).

Lp-CM Does Not Induce Increased Cell Death at Concentrations Used to Inhibit Endogenous NF-κB and Proteasome Activity

NF-_κ_B, while an important mediator of inflammation, also serves an important anti-apoptotic role in the gut.33,34 Therefore, the possibility that blockade of NF-_κ_B by Lp-CM would affect cell viability was investigated using an apoptosis cell death assay. Both RAW 264.7 macrophage cells and YAMC cells were treated with Lp-CM at the same dose and for the same time period used previously to inhibit NF-_κ_B activation and proteasome activity. In contrast to the proteasome inhibitor bortezomib (Velcade®), which is used clinically for the treatment of human hematologic malignancies,35 no toxicity was observed with Lp-CM after 4 hours of exposure (Fig. 9A). A time course of cell death with Lp-CM in YAMC cells from 0–8 hours is also shown (Fig. 9B). Even at 8 hours of exposure, there is less cell death seen with Lp-CM than with MG132, another proteasome inhibitor. However, when TNF-α is added after pretreatment with Lp-CM for 4 hours, there is increased cell death after addition of TNF-α (Fig. 9B). The amount of cell death after addition of TNF-α in the Lp-CM-treated cells was still significantly less than cells treated with the proteasome inhibitor MG132 after TNF-α, and was less than what was observed with CAM, a traditional inducer of apoptosis. No increase in cell death was seen after 4 hours when TNF-α was added prior to, or at the same time as Lp-CM (data not shown).

FIGURE 9.

FIGURE 9

Lp-CM does not induce increased cell death compared to other proteasome inhibitors. A: Lp-CM was used to treat YAMC cells or RAW cells for 4 hours and the effects of Lp-CM on cell death were determined using a commercially available cell death ELISA assay. The proteasome inhibitor bortezomib is also shown. Results are expressed as mean ± SD for 3 separate experiments. B: YAMC cells were pretreated with either MG132 or Lp-CM for the times indicated (0--8 hours). Cells pretreated with either MG132 or Lp-CM for 4 hours were then treated with 30 ng/mL TNF-α for an additional 4 hours and cell death measured by ELISA. Results are expressed as mean ± SD for 2 separate experiments, performed in triplicate.

DISCUSSION

Enteric flora play an essential role in the maintenance of intestinal homeostasis and gastrointestinal health.36–39 Interestingly, these critical roles in intestinal homeostasis appear largely mediated through TLR signaling.37,40 TLRs play a key role in innate immunity by recognizing various bacterial and viral products, thus forming the first line of defense in protecting the host against pathogens.41 However, TLRs do not discriminate between normal enteric flora and pathogens. They activate inflammatory pathways for host defense, but are concurrently critical for activating signaling pathways necessary for the maintenance of intestinal homeostasis.37,40

How the gut preserves this balance and maintains intestinal homeostasis, in the presence of a large bacterial load, remains the subject of intense study. Dysregulation of inflammatory signaling pathways, resulting in an aberrant immune response, is thought to play an important role in the development of inflammatory colitis both in humans and in animal models.42,43 Furthermore, studies of ulcerative colitis (UC) and Crohn’s disease (CD) have demonstrated that changes in the equilibrium of intestinal bacteria occur in areas of active inflammation, for example, fewer Bifidobacteria species and more Enterobactericiae species have been reported as being associated with active disease.6

Lactobacillus plantarum is one of the most common Lactobacillus species in the gut of healthy human volunteers.44 It is a member of the phylum Firmicutes, one of two major phyla that dominate the intestinal microbiota.45 In addition, live L. plantarum ameliorates disease and decreases inflammation in an IL-10 knockout mouse model of colitis46 as well as in a dextran sodium sulfate (DSS) rat model of inflammatory colitis.47 In this study we show that Lp-CM uniquely inhibits NF-_κ_B binding activity in response to TNF-α attenuates release of MCP-1, a proinflammatory chemokine and downstream gene target of NF-_κ_B, and directly, reversibly inhibits proteasome function. Lp-CM inhibited NF-_κ_B activation from TNF-receptor, MyD88-dependent, and MyD88-independent pathways, consistent with its downstream inhibitory effects on the proteasome. Despite its potent effects on proteasome function, Lp-CM resulted in less cell death and toxicity compared to other proteasome inhibitors, even in the presence of TNF-α.

The findings here raise the possibility that the absence of certain commensals important in controlling NF-_κ_B activation may predispose to the development of inflammatory conditions such as IBD. Studies both in humans and in animals have now indicated that modulation of proteasome function and proteasome subunit expression likely plays a role in colitis,31,48,49 and increased chymotrypsin-like proteasome activity has been reported in biopsies from patients with active CD.31 Our findings may explain why some probiotics which inhibit NF-_κ_B and proteasome function such as L. plantarum, a commensal and probiotic, and VSL#3, another probiotic mixture that has been used clinically to treat pouchitis, confer protection against intestinal inflammation.

Although in our screen only L. plantarum was found to possess these properties, it would be presumptuous to assume that only this bacteria possesses this unique ability. It is more likely that other commensals similarly affect NF-κ_B activation to assist the host in minimizing aberrant or overexuberant inflammatory responses. For example, it has been shown that live L. casei was able to inhibit I_κ_B_α degradation during Shigella infection.50 Another recent study51 showed that a decrease in the commensal bacterium Faecalibacterium prausnitzii was associated with endoscopic recurrence of ileal CD. Furthermore, the bacteria-derived supernatant was shown to possess anti-inflammatory properties both in vitro and in a TNBS animal model of colitis.51 Hence, it appears likely that certain commensals such as L. plantarum, F. prausnitzii, and others play a very active anti-inflammatory role.

The balance of gut pro- and anti-inflammatory signaling due to bacteria and host interplay may be what ultimately determines whether there is development of disease or whether intestinal homeostasis is maintained. As L. plantarum is a common commensal, it is tempting to speculate that the factors it produces in Lp-CM may possess anti-inflammatory properties that can block development of colitis, without compromising the ability of the host to fight off infection.

Interestingly, lipoteichoic acid from L. plantarum (pLTA) has been shown to exhibit inhibitory activity against Staphylococcus aureus LTA-induced TNF-α production, and to block NF-_κ_B activated by other TLR ligands such as LPS.52,53 In the case of pLTA, the mechanism of NF-_κ_B inhibition was found to be a downregulation of TLR4, NOD1, and NOD2 receptors.53 These studies, along with ours, further highlight the existence of multiple _L. plantarum_-derived factors with different bioactivity and antiinflammatory function. Future characterization of these various bioactive factors will further enhance our understanding of the crosstalk between commensal bacteria and the gut.

Acknowledgments

This work was performed in the Martin Boyer Research Laboratories at the University of Chicago and in the Gastrointestinal Diseases Research Unit laboratories (GIDRU) at Queen’s University. We thank Curtis Noordhof for expert technical assistance and Ian Spreadbury at Queen’s University for computer support. We also thank Dr. Stig Bengmark for generous gifts of the Lactobacillus strains used in this study, Gail Hecht for the EPEC strain of E. coli, Beth McCormick for the F18 strain of E. coli, and James Madara for the S. typhimurium strain.

Supported by the Crohn’s and Colitis Foundation of Canada (to E.O.P), and by National Institutes of Health grants DK064840 (to E.O.P), HD043839 (to E.C.C), AT004044 (to E.O.P. and E.C.C.), DK47722 (to E.B.C.), and Digestive Disease Research Core Center grant DK42086.

References