Identification of a Bacillus subtilis spo0H allele that is necessary for suppression of the sporulation-defective phenotype of a spo0A mutation (original) (raw)
Abstract
A mutation in Bacillus subtilis spo0A codon 97 suppressed the sporulation defect caused by the spo0A9V mutation. The suppressor activity of the codon 97 mutation was evident only in the presence of a novel spo0H allele. Our results suggest that the spo0A gene product interacts with the sigma factor subunit of RNA polymerase.
Full Text
The Full Text of this article is available as a PDF (205.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benson A. K., Haldenwang W. G. Regulation of sigma B levels and activity in Bacillus subtilis. J Bacteriol. 1993 Apr;175(8):2347–2356. doi: 10.1128/jb.175.8.2347-2356.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burbulys D., Trach K. A., Hoch J. A. Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay. Cell. 1991 Feb 8;64(3):545–552. doi: 10.1016/0092-8674(91)90238-t. [DOI] [PubMed] [Google Scholar]
- Daniels D., Zuber P., Losick R. Two amino acids in an RNA polymerase sigma factor involved in the recognition of adjacent base pairs in the -10 region of a cognate promoter. Proc Natl Acad Sci U S A. 1990 Oct;87(20):8075–8079. doi: 10.1073/pnas.87.20.8075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubnau E., Weir J., Nair G., Carter L., 3rd, Moran C., Jr, Smith I. Bacillus sporulation gene spo0H codes for sigma 30 (sigma H). J Bacteriol. 1988 Mar;170(3):1054–1062. doi: 10.1128/jb.170.3.1054-1062.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Errington J., Jones D. Cloning in Bacillus subtilis by transfection with bacteriophage vector phi 105J27: isolation and preliminary characterization of transducing phages for 23 sporulation loci. J Gen Microbiol. 1987 Mar;133(3):493–502. doi: 10.1099/00221287-133-3-493. [DOI] [PubMed] [Google Scholar]
- Ferrari F. A., Trach K., LeCoq D., Spence J., Ferrari E., Hoch J. A. Characterization of the spo0A locus and its deduced product. Proc Natl Acad Sci U S A. 1985 May;82(9):2647–2651. doi: 10.1073/pnas.82.9.2647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frisby D., Zuber P. Analysis of the upstream activating sequence and site of carbon and nitrogen source repression in the promoter of an early-induced sporulation gene of Bacillus subtilis. J Bacteriol. 1991 Dec;173(23):7557–7564. doi: 10.1128/jb.173.23.7557-7564.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grossman A. D., Lewis T., Levin N., DeVivo R. Suppressors of a spo0A missense mutation and their effects on sporulation in Bacillus subtilis. Biochimie. 1992 Jul-Aug;74(7-8):679–688. doi: 10.1016/0300-9084(92)90140-a. [DOI] [PubMed] [Google Scholar]
- Guzmán P., Westpheling J., Youngman P. Characterization of the promoter region of the Bacillus subtilis spoIIE operon. J Bacteriol. 1988 Apr;170(4):1598–1609. doi: 10.1128/jb.170.4.1598-1609.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Helmann J. D., Chamberlin M. J. Structure and function of bacterial sigma factors. Annu Rev Biochem. 1988;57:839–872. doi: 10.1146/annurev.bi.57.070188.004203. [DOI] [PubMed] [Google Scholar]
- Kenney T. J., York K., Youngman P., Moran C. P., Jr Genetic evidence that RNA polymerase associated with sigma A factor uses a sporulation-specific promoter in Bacillus subtilis. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9109–9113. doi: 10.1073/pnas.86.23.9109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kudoh J., Ikeuchi T., Kurahashi K. Nucleotide sequences of the sporulation gene spo0A and its mutant genes of Bacillus subtilis. Proc Natl Acad Sci U S A. 1985 May;82(9):2665–2668. doi: 10.1073/pnas.82.9.2665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olmedo G., Ninfa E. G., Stock J., Youngman P. Novel mutations that alter the regulation of sporulation in Bacillus subtilis. Evidence that phosphorylation of regulatory protein SpoOA controls the initiation of sporulation. J Mol Biol. 1990 Oct 5;215(3):359–372. doi: 10.1016/s0022-2836(05)80357-2. [DOI] [PubMed] [Google Scholar]
- Perego M., Wu J. J., Spiegelman G. B., Hoch J. A. Mutational dissociation of the positive and negative regulatory properties of the Spo0A sporulation transcription factor of Bacillus subtilis. Gene. 1991 Apr;100:207–212. doi: 10.1016/0378-1119(91)90368-l. [DOI] [PubMed] [Google Scholar]
- Satola S. W., Baldus J. M., Moran C. P., Jr Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis. J Bacteriol. 1992 Mar;174(5):1448–1453. doi: 10.1128/jb.174.5.1448-1453.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schuch R., Piggot P. J. The dacF-spoIIA operon of Bacillus subtilis, encoding sigma F, is autoregulated. J Bacteriol. 1994 Jul;176(13):4104–4110. doi: 10.1128/jb.176.13.4104-4110.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strauch M. A., Trach K. A., Day J., Hoch J. A. Spo0A activates and represses its own synthesis by binding at its dual promoters. Biochimie. 1992 Jul-Aug;74(7-8):619–626. doi: 10.1016/0300-9084(92)90133-y. [DOI] [PubMed] [Google Scholar]
- Strauch M., Webb V., Spiegelman G., Hoch J. A. The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene. Proc Natl Acad Sci U S A. 1990 Mar;87(5):1801–1805. doi: 10.1073/pnas.87.5.1801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trach K., Burbulys D., Strauch M., Wu J. J., Dhillon N., Jonas R., Hanstein C., Kallio P., Perego M., Bird T. Control of the initiation of sporulation in Bacillus subtilis by a phosphorelay. Res Microbiol. 1991 Sep-Oct;142(7-8):815–823. doi: 10.1016/0923-2508(91)90060-n. [DOI] [PubMed] [Google Scholar]
- Wu J. J., Howard M. G., Piggot P. J. Regulation of transcription of the Bacillus subtilis spoIIA locus. J Bacteriol. 1989 Feb;171(2):692–698. doi: 10.1128/jb.171.2.692-698.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- York K., Kenney T. J., Satola S., Moran C. P., Jr, Poth H., Youngman P. Spo0A controls the sigma A-dependent activation of Bacillus subtilis sporulation-specific transcription unit spoIIE. J Bacteriol. 1992 Apr;174(8):2648–2658. doi: 10.1128/jb.174.8.2648-2658.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zuber P., Healy J., Carter H. L., 3rd, Cutting S., Moran C. P., Jr, Losick R. Mutation changing the specificity of an RNA polymerase sigma factor. J Mol Biol. 1989 Apr 20;206(4):605–614. doi: 10.1016/0022-2836(89)90569-x. [DOI] [PubMed] [Google Scholar]
- Zuber P., Losick R. Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation. Cell. 1983 Nov;35(1):275–283. doi: 10.1016/0092-8674(83)90230-1. [DOI] [PubMed] [Google Scholar]