Rajesh Rastogi | Sardar Patel University, Vallabh Vidyanagar, India (original) (raw)

Papers by Rajesh Rastogi

Australian Journal of Botany

The cyanobacterium Scytonema sp. HKAR-3 isolated from a hot spring in India was investigated for ... more The cyanobacterium Scytonema sp. HKAR-3 isolated from a hot spring in India was investigated for the presence of mycosporine-like amino acids (MAAs) and their induction under PAR and PAR+UVR. High-performance liquid-chromatograph (HPLC) analysis revealed the presence of two MAAs, mycosporine–glycine (λmax = 310 nm) and an unknown MAA-334 (λmax = 334 nm), with retention times of 4.1 and 8.7 min, respectively. This is the first report for the presence of two MAAs and, in particular, the synthesis of mycosporine–glycine in any strain of Scytonema. There was no effect of radiation type (PAR or PAR+UVR) on the synthesis of mycosporine–glycine; however, the synthesis of MAA-334 was found to be higher in samples receiving PAR+UVR than in those receiving PAR only. There was a circadian induction in the synthesis of MAAs under alternate 12-h light (PAR or PAR+UVR) and dark periods. MAAs were found to be induced mostly during the light period and their production dropped again during the dark...

Renewable and Sustainable Energy Reviews

Photosynthesis research, Jan 24, 2018

Cyanobacteria exhibit a novel form of non-photochemical quenching (NPQ) at the level of the phyco... more Cyanobacteria exhibit a novel form of non-photochemical quenching (NPQ) at the level of the phycobilisome. NPQ is a process that protects photosystem II (PSII) from possible highlight-induced photo-damage. Although significant advancement has been made in understanding the NPQ, there are still some missing details. This critical review focuses on how the orange carotenoid protein (OCP) and its partner fluorescence recovery protein (FRP) control the extent of quenching. What is and what is not known about the NPQ is discussed under four subtitles; where does exactly the site of quenching lie? (site), how is the quenching being triggered? (trigger), molecular mechanism of quenching (quenching) and recovery from quenching. Finally, a recent working model of NPQ, consistent with recent findings, is been described.

Photosynthesis research, Jan 23, 2018

In the original publication, under the subtitle Recovery: fluorescence recovery protein (FRP), pa... more In the original publication, under the subtitle Recovery: fluorescence recovery protein (FRP), paragraph 4 the text section enclosed in quotation marks does not occur in one of the original publications cited (Sluchanko et al. 2017a, b).

The Science of the total environment, Jan 15, 2018

To explore the potential genes from the industrially polluted Amlakhadi canal, located in Anklesh... more To explore the potential genes from the industrially polluted Amlakhadi canal, located in Ankleshwar, Gujarat, India, its community genome was extracted and cloned into E. coli EPI300™-T1 using a fosmid vector (pCC2 FOS™) generating a library of 3,92,000 clones with average size of 40kb of DNA-insert. From this library, the clone DM1 producing brown colored melanin-like pigment was isolated and characterized. For over expression of the pigment, further sub-cloning of the clone DM1 was done. Sub-clone containing 10kb of the insert was sequenced for gene identification. The amino acids sequence of a protein 4-Hydroxyphenylpyruvate dioxygenase (HPPD), which is know to be involved in melanin biosynthesis was obtained from the gene sequence. The sequence-homology based 3D structure model of HPPD was constructed and analyzed. The physico-chemical nature of pigment was further analysed using H and C NMR, LC-MS, FTIR and UV-visible spectroscopy. The pigment was readily soluble in DMSO with ...

Bioresource Technology

Bio-hydrogen from microalgae including cyanobacteria has attracted commercial awareness due to it... more Bio-hydrogen from microalgae including cyanobacteria has attracted commercial awareness due to its potential as an alternative, reliable and renewable energy source. Photosynthetic hydrogen production from microalgae can be interesting and promising options for clean energy. Advances in hydrogen-fuel-cell technology may attest an eco-friendly way of biofuel production, since, the use of H2 to generate electricity releases only water as a by-product. Progress in genetic/metabolic engineering may significantly enhance the photobiological hydrogen production from microalgae. Manipulation of competing metabolic pathways by modulating the certain key enzymes such as hydrogenase and nitrogenase may enhance the evolution of H2 from photoautotrophic cells. Moreover, biological H2 production at low operating costs is requisite for economic viability. Several photobioreactors have been developed for large-scale biomass and hydrogen production. This review highlights the recent technological progress, enzymes involved and genetic as well as metabolic engineering approaches towards sustainable hydrogen production from microalgae.

Bioenergetics: Open Access, 2016

ABSTRACT The global climate change with an increase in solar ultraviolet (UV) radiations on Earth... more ABSTRACT The global climate change with an increase in solar ultraviolet (UV) radiations on Earth’s surface has generated tremendous concern about its negative impact on all sun-exposed photosynthetic life-forms including cyanobacteria. UV (280 - 400 nm) can affect a number of physiological and biochemical processes in cyanobacteria either directly or indirectly by the generation of reactive oxygen species. Drastic effects caused by UV radiation may imbalance the entire ecosystems. However, many cyanobacteria are able to develop several defense mechanisms against the damaging effects imposed by increased UV radiation. These strategies include the restoration of genetic material by means of excision repair and photoreactivation, repair and re-synthesis of D1 and D2 proteins of PSII reaction center, activation of antioxidant systems, and biosynthesis of several photoprotectants such as mycosporine-like amino acids, scytonemin, carotenoids and polyamines. This chapter presents an overview on the current knowledge of the physiological as well as biochemical adaptation of cyanobacteria with regard to the function of potent defense mechanisms in response to intense solar UV radiation.

Protoplasma, Jan 22, 2016

In vitro antioxidant virtue and life-prolonging effect of phycoerythrin (PE; a pigment protein is... more In vitro antioxidant virtue and life-prolonging effect of phycoerythrin (PE; a pigment protein isolated from Phormidium sp. A09DM) have been revealed in our previous reports (Sonani et al. in Age 36:9717, 2014a; Sonani et al. in Process Biochem 49:1757-1766, 2014b). It has been hypothesized that the PE expands life span of Caenorhabditis elegans (bears large resemblance with human aging pathways) due to its antioxidant virtue. This hypothesis is tested in present study by checking the effect of PE on intracellular reactive oxygen species (ROS) generation and associated physiological deformities using mouse and human skin fibroblasts, C. elegans, and Drosophila melanogaster Oregon R (+) and by divulging PE's structural attributes responsible for its antioxidant asset. PE treatment displayed noteworthy decrease of 67, 48, and 77 % in ROS level in mouse fibroblast (3T3-L1), human fibroblast, and C. elegans N2, respectively, arisen under chemical-induced oxidative stress. PE treatme...

Biochemistry & Analytical Biochemistry, 2015

World Journal of Biological Chemistry, 2016

An obligatory sunlight requirement for photosynthesis has exposed cyanobacteria to different quan... more An obligatory sunlight requirement for photosynthesis has exposed cyanobacteria to different quantity and quality of light. Cyanobacteria can exhibit efficient photosynthesis over broad region (450 to 650 nm) of solar spectrum with the help of brilliantly coloured pigment proteins called phycobiliproteins (PBPs). Besides light-harvesting, PBPs are found to involve in several life sustaining phenomena including photoprotection in cyanobacteria. The unique spectral features (like strong absorbance and fluorescence), proteineous nature and, some imperative properties like hepato-protective, anti-oxidants, anti-inflammatory and anti-aging activity of PBPs enable their use in food, cosmetics, pharmaceutical and biomedical industries. PBPs have been also noted to show beneficial effect in therapeutics of some disease like Alzheimer and cancer. Such large range of applications increases the demand of PBPs in commodity market. Therefore, the large-scale and coast effective production of PBPs is the real need of time. To fulfil this need, many researchers have been working to find the potential producer of PBPs for the production and purification of PBPs. Results of these efforts have caused the inventions of some novel techniques like mixotrophic and heterotrophic strategies for production and aqueous two phase separation for purification purpose. Overall, the present review summarises the recent findings and identifies gaps in the field of production, purification and applications of this biological and economically important proteins.

Frontiers in Microbiology, 2015

Cyanobacteria are ecologically one of the most prolific groups of phototrophic prokaryotes in bot... more Cyanobacteria are ecologically one of the most prolific groups of phototrophic prokaryotes in both marine and freshwater habitats. Both the beneficial and detrimental aspects of cyanobacteria are of considerable significance. They are important primary producers as well as an immense source of several secondary products, including an array of toxic compounds known as cyanotoxins. Abundant growth of cyanobacteria in freshwater, estuarine, and coastal ecosystems due to increased anthropogenic eutrophication and global climate change has created serious concern toward harmful bloom formation and surface water contamination all over the world. Cyanobacterial blooms and the accumulation of several cyanotoxins in water bodies pose severe ecological consequences with high risk to aquatic organisms and global public health. The proper management for mitigating the worldwide incidence of toxic cyanobacterial blooms is crucial for maintenance and sustainable development of functional ecosystems. Here, we emphasize the emerging information on the cyanobacterial bloom dynamics, toxicology of major groups of cyanotoxins, as well as a perspective and integrative approach to their management.

EXCLI journal, 2015

Cyanobacteria trap light energy by arrays of pigment molecules termed "phycobilisomes (PBSs)... more Cyanobacteria trap light energy by arrays of pigment molecules termed "phycobilisomes (PBSs)", organized proximal to "reaction centers" at which chlorophyll perform the energy transduction steps with highest quantum efficiency. PBSs, composed of sequential assembly of various chromophorylated phycobiliproteins (PBPs), as well as nonchromophoric, basic and hydrophobic polypeptides called linkers. Atomic resolution structure of PBP is a heterodimer of two structurally related polypeptides but distinct specialised polypeptides- a and ß, made up of seven alpha-helices each which played a crucial step in evolution of PBPs. PBPs carry out various light dependent responses such as complementary chromatic adaptation. The aim of this review is to summarize and discuss the recent progress in this field and to highlight the new and the questions that remain unresolved.

RSC Adv., 2015

We report the occurrence of a functionally stable single peptide APC α-subunit in cyanobacterium ... more We report the occurrence of a functionally stable single peptide APC α-subunit in cyanobacterium Nostoc sp. R76DM.

The correct execution of the DNA replication process is crucially important for the maintenance o... more The correct execution of the DNA replication process is crucially important for the maintenance of genome integrity of the cell. Several types of sources, both endogenous and exogenous, can give rise to DNA damage. The most potent carcinogenic forms of UV-induced DNA lesions are CPDs, 6-4PPs and their Dewar isomers. The incidence of UVR, IR and certain genotoxic chemicals may result in single as well as double DNA strand breaks (DSBs) leading to arrest of DNA replication fork that may impede with normal cellular capability and functional integrity, reduction of RNA synthesis, arrest of cell cycle progression, mutagenesis, tumorigenesis and apoptosis. A number of detection strategies have been devised to detect different types of DNA lesions. To overcome the above malfunctions, nature has invented certain specific repair mechanisms such as photoreactivation, excision repair, mismatch repair (MMR), double strand break (DSB) repair and certain other mechanisms like damage tolerance (dimer bypass), prokaryotic SOS response and cell cycle checkpoint activation in organisms. The defects in repair machinery may alter the configuration of cell structure, lipids, proteins and nucleic acids and can cause a number of human diseases.