A processive single-headed motor: kinesin superfamily protein KIF1A - PubMed (original) (raw)
A processive single-headed motor: kinesin superfamily protein KIF1A
Y Okada et al. Science. 1999.
Abstract
A single kinesin molecule can move "processively" along a microtubule for more than 1 micrometer before detaching from it. The prevailing explanation for this processive movement is the "walking model," which envisions that each of two motor domains (heads) of the kinesin molecule binds coordinately to the microtubule. This implies that each kinesin molecule must have two heads to "walk" and that a single-headed kinesin could not move processively. Here, a motor-domain construct of KIF1A, a single-headed kinesin superfamily protein, was shown to move processively along the microtubule for more than 1 micrometer. The movement along the microtubules was stochastic and fitted a biased Brownian-movement model.
Similar articles
- Conversion of Unc104/KIF1A kinesin into a processive motor after dimerization.
Tomishige M, Klopfenstein DR, Vale RD. Tomishige M, et al. Science. 2002 Sep 27;297(5590):2263-7. doi: 10.1126/science.1073386. Science. 2002. PMID: 12351789 - 15 A resolution model of the monomeric kinesin motor, KIF1A.
Kikkawa M, Okada Y, Hirokawa N. Kikkawa M, et al. Cell. 2000 Jan 21;100(2):241-52. doi: 10.1016/s0092-8674(00)81562-7. Cell. 2000. PMID: 10660047 - Intracellular transport of single-headed molecular motors KIF1A.
Nishinari K, Okada Y, Schadschneider A, Chowdhury D. Nishinari K, et al. Phys Rev Lett. 2005 Sep 9;95(11):118101. doi: 10.1103/PhysRevLett.95.118101. Epub 2005 Sep 7. Phys Rev Lett. 2005. PMID: 16197050 - Directional motility of kinesin motor proteins.
Woehlke G, Schliwa M. Woehlke G, et al. Biochim Biophys Acta. 2000 Mar 17;1496(1):117-27. doi: 10.1016/s0167-4889(00)00013-6. Biochim Biophys Acta. 2000. PMID: 10722881 Review. - Kinesin: a molecular motor with a spring in its step.
Thomas N, Imafuku Y, Kamiya T, Tawada K. Thomas N, et al. Proc Biol Sci. 2002 Nov 22;269(1507):2363-71. doi: 10.1098/rspb.2002.2117. Proc Biol Sci. 2002. PMID: 12495505 Free PMC article. Review.
Cited by
- Processivity of the kinesin-2 KIF3A results from rear head gating and not front head gating.
Chen GY, Arginteanu DF, Hancock WO. Chen GY, et al. J Biol Chem. 2015 Apr 17;290(16):10274-94. doi: 10.1074/jbc.M114.628032. Epub 2015 Feb 5. J Biol Chem. 2015. PMID: 25657001 Free PMC article. - Molecular crowding creates traffic jams of kinesin motors on microtubules.
Leduc C, Padberg-Gehle K, Varga V, Helbing D, Diez S, Howard J. Leduc C, et al. Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6100-5. doi: 10.1073/pnas.1107281109. Epub 2012 Mar 19. Proc Natl Acad Sci U S A. 2012. PMID: 22431622 Free PMC article. - Variable surface loops and myosin activity: accessories to a motor.
Murphy CT, Spudich JA. Murphy CT, et al. J Muscle Res Cell Motil. 2000 Feb;21(2):139-51. doi: 10.1023/a:1005610007209. J Muscle Res Cell Motil. 2000. PMID: 10961838 Review. - Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin.
Okada Y, Hirokawa N. Okada Y, et al. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):640-5. doi: 10.1073/pnas.97.2.640. Proc Natl Acad Sci U S A. 2000. PMID: 10639132 Free PMC article. - High-resolution cryo-EM maps show the nucleotide binding pocket of KIF1A in open and closed conformations.
Kikkawa M, Hirokawa N. Kikkawa M, et al. EMBO J. 2006 Sep 20;25(18):4187-94. doi: 10.1038/sj.emboj.7601299. Epub 2006 Aug 31. EMBO J. 2006. PMID: 16946706 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases