Voltage sensors in domains III and IV, but not I and II, are immobilized by Na+ channel fast inactivation - PubMed (original) (raw)
Voltage sensors in domains III and IV, but not I and II, are immobilized by Na+ channel fast inactivation
A Cha et al. Neuron. 1999 Jan.
Free article
Abstract
Using site-directed fluorescent labeling, we examined conformational changes in the S4 segment of each domain of the human skeletal muscle sodium channel (hSkM1). The fluorescence signals from S4 segments in domains I and II follow activation and are unaffected as fast inactivation settles. In contrast, the fluorescence signals from S4 segments in domains III and IV show kinetic components during activation and deactivation that correlate with fast inactivation and charge immobilization. These results indicate that in hSkM1, the S4 segments in domains III and IV are responsible for voltage-sensitive conformational changes linked to fast inactivation and are immobilized by fast inactivation, while the S4 segments in domains I and II are unaffected by fast inactivation.
Comment in
- Light at the end of the channel.
Miller C. Miller C. Neuron. 1999 Jan;22(1):1-2. doi: 10.1016/s0896-6273(00)80667-4. Neuron. 1999. PMID: 10027278 No abstract available.
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