Differential addressing of 5-HT1A and 5-HT1B receptors in epithelial cells and neurons - PubMed (original) (raw)
. 1999 Mar:112 ( Pt 6):967-76.
doi: 10.1242/jcs.112.6.967.
Affiliations
- PMID: 10036246
- DOI: 10.1242/jcs.112.6.967
Differential addressing of 5-HT1A and 5-HT1B receptors in epithelial cells and neurons
A Ghavami et al. J Cell Sci. 1999 Mar.
Abstract
The 5-HT1A and 5-HT1B serotonin receptors are expressed in a variety of neurons in the central nervous system. While the 5-HT1A receptor is found on somas and dendrites, the 5-HT1B receptor has been suggested to be localized predominantly on axon terminals. To study the intracellular addressing of these receptors, we have used in vitro systems including Madin-Darby canine kidney (MDCK II) epithelial cells and primary neuronal cultures. Furthermore, we have extended these studies to examine addressing in vivo in transgenic mice. In epithelial cells, 5-HT1A receptors are found on both apical and basolateral membranes while 5-HT1B receptors are found exclusively in intracellular vesicles. In hippocampal neuronal cultures, 5-HT1A receptors are expressed on somatodendritic membranes but are absent from axons. In contrast, 5-HT1B receptors are found on both dendritic and axonal membranes, including growth cones where they accumulate. Using 5-HT1A and 5-HT1B knockout mice and the binary tTA/tetO system, we generated mice expressing these receptors in striatal neurons. These in vivo experiments demonstrate that, in striatal medium spiny neurons, the 5-HT1A receptor is restricted to the somatodendritic level, while 5-HT1B receptors are shipped exclusively toward axon terminals. Therefore, in all systems we have examined, there is a differential sorting of the 5-HT1A and 5-HT1B receptors. Furthermore, we conclude that our in vivo transgenic system is the only model that reconstitutes proper sorting of these receptors.
Similar articles
- Regional changes in density of serotonin transporter in the brain of 5-HT1A and 5-HT1B knockout mice, and of serotonin innervation in the 5-HT1B knockout.
Ase AR, Reader TA, Hen R, Riad M, Descarries L. Ase AR, et al. J Neurochem. 2001 Aug;78(3):619-30. doi: 10.1046/j.1471-4159.2001.00437.x. J Neurochem. 2001. PMID: 11483665 - 5-HT1A and 5-HT1B receptors control the firing of serotoninergic neurons in the dorsal raphe nucleus of the mouse: studies in 5-HT1B knock-out mice.
Evrard A, Laporte AM, Chastanet M, Hen R, Hamon M, Adrien J. Evrard A, et al. Eur J Neurosci. 1999 Nov;11(11):3823-31. doi: 10.1046/j.1460-9568.1999.00800.x. Eur J Neurosci. 1999. PMID: 10583471 - Somatodendritic localization of 5-HT1A and preterminal axonal localization of 5-HT1B serotonin receptors in adult rat brain.
Riad M, Garcia S, Watkins KC, Jodoin N, Doucet E, Langlois X, el Mestikawy S, Hamon M, Descarries L. Riad M, et al. J Comp Neurol. 2000 Feb 7;417(2):181-94. J Comp Neurol. 2000. PMID: 10660896 - Altered emotional states in knockout mice lacking 5-HT1A or 5-HT1B receptors.
Zhuang X, Gross C, Santarelli L, Compan V, Trillat AC, Hen R. Zhuang X, et al. Neuropsychopharmacology. 1999 Aug;21(2 Suppl):52S-60S. doi: 10.1016/S0893-133X(99)00047-0. Neuropsychopharmacology. 1999. PMID: 10432489 Review. - Serotonin receptors in brain revisited.
Palacios JM. Palacios JM. Brain Res. 2016 Aug 15;1645:46-9. doi: 10.1016/j.brainres.2015.12.042. Epub 2015 Dec 29. Brain Res. 2016. PMID: 26740406 Review.
Cited by
- Development of 2-Aminotetralin-Type Serotonin 5-HT1 Agonists: Molecular Determinants for Selective Binding and Signaling at 5-HT1A, 5-HT1B, 5-HT1D, and 5-HT1F Receptors.
McGlynn RP, Cui M, Brems B, Holbrook O, Booth RG. McGlynn RP, et al. ACS Chem Neurosci. 2024 Jan 17;15(2):357-370. doi: 10.1021/acschemneuro.3c00658. Epub 2023 Dec 27. ACS Chem Neurosci. 2024. PMID: 38150333 Free PMC article. - The Effect of Serotonin Receptor 5-HT1B on Lateral Inhibition between Spiny Projection Neurons in the Mouse Striatum.
Pommer S, Akamine Y, Schiffmann SN, de Kerchove d'Exaerde A, Wickens JR. Pommer S, et al. J Neurosci. 2021 Sep 15;41(37):7831-7847. doi: 10.1523/JNEUROSCI.1037-20.2021. Epub 2021 Aug 4. J Neurosci. 2021. PMID: 34348999 Free PMC article. - Serotonin-mediated inhibition of ventral hippocampus is required for sustained goal-directed behavior.
Yoshida K, Drew MR, Mimura M, Tanaka KF. Yoshida K, et al. Nat Neurosci. 2019 May;22(5):770-777. doi: 10.1038/s41593-019-0376-5. Epub 2019 Apr 15. Nat Neurosci. 2019. PMID: 30988523 - 5-HT1B Receptor-Mediated Activation of ERK1/2 Requires Both Gαi/o and β-Arrestin Proteins.
Liu Y, Gibson AW, Levinstein MR, Lesiak AJ, Ong SE, Neumaier JF. Liu Y, et al. ACS Chem Neurosci. 2019 Jul 17;10(7):3143-3153. doi: 10.1021/acschemneuro.8b00596. Epub 2019 Apr 29. ACS Chem Neurosci. 2019. PMID: 30946562 Free PMC article. - Serotonin receptors in depression: from A to B.
Nautiyal KM, Hen R. Nautiyal KM, et al. F1000Res. 2017 Feb 9;6:123. doi: 10.12688/f1000research.9736.1. eCollection 2017. F1000Res. 2017. PMID: 28232871 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases