Characterization of inositol-1,4,5-trisphosphate-gated channels in the plasma membrane of rat olfactory neurons - PubMed (original) (raw)
Characterization of inositol-1,4,5-trisphosphate-gated channels in the plasma membrane of rat olfactory neurons
F W Lischka et al. Biophys J. 1999 Mar.
Abstract
It is generally accepted that inositol-1,4,5-trisphosphate (InsP3) plays a role in olfactory transduction. However, the precise mode of action of InsP3 remains controversial. We have characterized the conductances activated by the addition of 10 microM InsP3 to excised patches of soma plasma membrane from rat olfactory neurons. InsP3 induced current fluctuations in 25 of 121 inside-out patches. These conductances could be classified into two groups according to the polarity of the current at a holding potential of +40 to +60 mV (with Ringer's in the pipette and pseudointracellular solution in the bath). Conductances mediating outward currents could be further divided into large- (64 +/- 4 pS, n = 4) and small- (16 +/- 1.7 pS, n = 11) conductance channels. Both small- and large-conductance channels were nonspecific cation channels. The large-conductance channel displayed bursting behavior at +40 mV, with flickering increasing at negative holding potentials to the point where single-channel currents were no longer discernible. The small-conductance channel did not display flickering behavior. The conductance mediating inward currents at +40 to +60 mV reversed at +73 +/- 4 mV (n = 4). The current traces displayed considerable fluctuations, and single-channel currents could not be discerned. The current fluctuations returned to baseline after removal of InsP3. The power density spectrum for the excess noise generated by InsP3 followed a 1/f dependence consistent with conductance fluctuations in the channel mediating this current, although other mechanisms are not excluded. These experiments demonstrate the presence of plasma membrane InsP3-gated channels of different ionic specificity in olfactory receptor cells.
Similar articles
- IP3-gated channels and their occurrence relative to CNG channels in the soma and dendritic knob of rat olfactory receptor neurons.
Kaur R, Zhu XO, Moorhouse AJ, Barry PH. Kaur R, et al. J Membr Biol. 2001 May 15;181(2):91-105. J Membr Biol. 2001. PMID: 11420596 - InsP3-gated ion channels in rat olfactory cilia membrane.
Honda E, Teeter JH, Restrepo D. Honda E, et al. Brain Res. 1995 Dec 12;703(1-2):79-85. doi: 10.1016/0006-8993(95)01072-6. Brain Res. 1995. PMID: 8719618 - Basic properties of an inositol 1,4,5-trisphosphate-gated channel in carp olfactory cilia.
Cadiou H, Sienaert I, Vanlingen S, Parys JB, Molle G, Duclohier H. Cadiou H, et al. Eur J Neurosci. 2000 Aug;12(8):2805-11. doi: 10.1046/j.1460-9568.2000.00166.x. Eur J Neurosci. 2000. PMID: 10971622 - Ciliary cation conductances in olfactory receptor cells of the clawed toad Xenopus laevis.
Schild D. Schild D. EXS. 1993;66:165-71. doi: 10.1007/978-3-0348-7327-7_12. EXS. 1993. PMID: 7505648 Review. - Direct ion channel gating: a new function for intracellular messengers.
Hockberger PE, Swandulla D. Hockberger PE, et al. Cell Mol Neurobiol. 1987 Sep;7(3):229-36. doi: 10.1007/BF00711301. Cell Mol Neurobiol. 1987. PMID: 2449964 Review.
Cited by
- Inositol 1,4,5-trisphosphate receptor type 1 autoantibody (ITPR1-IgG/anti-Sj)-associated autoimmune cerebellar ataxia, encephalitis and peripheral neuropathy: review of the literature.
Jarius S, Bräuninger S, Chung HY, Geis C, Haas J, Komorowski L, Wildemann B, Roth C. Jarius S, et al. J Neuroinflammation. 2022 Jul 30;19(1):196. doi: 10.1186/s12974-022-02545-4. J Neuroinflammation. 2022. PMID: 35907972 Free PMC article. Review. - Inositol 1,4,5-trisphosphate receptor type 1 autoantibodies in paraneoplastic and non-paraneoplastic peripheral neuropathy.
Jarius S, Ringelstein M, Haas J, Serysheva II, Komorowski L, Fechner K, Wandinger KP, Albrecht P, Hefter H, Moser A, Neuen-Jacob E, Hartung HP, Wildemann B, Aktas O. Jarius S, et al. J Neuroinflammation. 2016 Oct 24;13(1):278. doi: 10.1186/s12974-016-0737-x. J Neuroinflammation. 2016. PMID: 27776522 Free PMC article. - 'Medusa-head ataxia': the expanding spectrum of Purkinje cell antibodies in autoimmune cerebellar ataxia. Part 1: Anti-mGluR1, anti-Homer-3, anti-Sj/ITPR1 and anti-CARP VIII.
Jarius S, Wildemann B. Jarius S, et al. J Neuroinflammation. 2015 Sep 17;12:166. doi: 10.1186/s12974-015-0356-y. J Neuroinflammation. 2015. PMID: 26377085 Free PMC article. Review. - Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia.
Jarius S, Scharf M, Begemann N, Stöcker W, Probst C, Serysheva II, Nagel S, Graus F, Psimaras D, Wildemann B, Komorowski L. Jarius S, et al. J Neuroinflammation. 2014 Dec 11;11:206. doi: 10.1186/s12974-014-0206-3. J Neuroinflammation. 2014. PMID: 25498830 Free PMC article. - Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in mouse olfactory neurons.
Szebenyi SA, Ogura T, Sathyanesan A, AlMatrouk AK, Chang J, Lin W. Szebenyi SA, et al. Front Cell Neurosci. 2014 Oct 21;8:336. doi: 10.3389/fncel.2014.00336. eCollection 2014. Front Cell Neurosci. 2014. PMID: 25374507 Free PMC article.
References
- Neuron. 1996 Oct;17(4):681-93 - PubMed
- J Gen Physiol. 1996 Mar;107(3):381-97 - PubMed
- Biophys J. 1997 Jul;73(1):168-78 - PubMed
- Biophys J. 1997 Dec;73(6):3349-57 - PubMed
- Physiol Rev. 1998 Apr;78(2):429-66 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources