A mammalian protein with specific demethylase activity for mCpG DNA - PubMed (original) (raw)

. 1999 Feb 18;397(6720):579-83.

doi: 10.1038/17533.

Affiliations

• PMID: 10050851
• DOI: 10.1038/17533

A mammalian protein with specific demethylase activity for mCpG DNA

S K Bhattacharya et al. Nature. 1999.

Abstract

DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.

PubMed Disclaimer

Comment in

• Gene expression. The amazing demethylase.
  Cedar H, Verdine GL. Cedar H, et al. Nature. 1999 Feb 18;397(6720):568-9. doi: 10.1038/17492. Nature. 1999. PMID: 10050847 No abstract available.

Similar articles

• Gene expression. The amazing demethylase.
  Cedar H, Verdine GL. Cedar H, et al. Nature. 1999 Feb 18;397(6720):568-9. doi: 10.1038/17492. Nature. 1999. PMID: 10050847 No abstract available.
• DNA methylation is a reversible biological signal.
  Ramchandani S, Bhattacharya SK, Cervoni N, Szyf M. Ramchandani S, et al. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6107-12. doi: 10.1073/pnas.96.11.6107. Proc Natl Acad Sci U S A. 1999. PMID: 10339549 Free PMC article.
• DNA methylation and chromatin structure.
  Lewis J, Bird A. Lewis J, et al. FEBS Lett. 1991 Jul 22;285(2):155-9. doi: 10.1016/0014-5793(91)80795-5. FEBS Lett. 1991. PMID: 1855583 Review.
• Structure, function and regulation of mammalian DNA methyltransferase.
  Leonhardt H, Bestor TH. Leonhardt H, et al. EXS. 1993;64:109-19. doi: 10.1007/978-3-0348-9118-9_5. EXS. 1993. PMID: 8418946 Review. No abstract available.

Cited by

• Modifiers and Readers of DNA Modifications and Their Impact on Genome Structure, Expression, and Stability in Disease.
  Ludwig AK, Zhang P, Cardoso MC. Ludwig AK, et al. Front Genet. 2016 Jun 21;7:115. doi: 10.3389/fgene.2016.00115. eCollection 2016. Front Genet. 2016. PMID: 27446199 Free PMC article. Review.
• Dynamic alterations in the paternal epigenetic landscape following fertilization.
  Jenkins TG, Carrell DT. Jenkins TG, et al. Front Genet. 2012 Jul 31;3:143. doi: 10.3389/fgene.2012.00143. eCollection 2012. Front Genet. 2012. PMID: 23024648 Free PMC article.
• CpG binding protein is crucial for early embryonic development.
  Carlone DL, Skalnik DG. Carlone DL, et al. Mol Cell Biol. 2001 Nov;21(22):7601-6. doi: 10.1128/MCB.21.22.7601-7606.2001. Mol Cell Biol. 2001. PMID: 11604496 Free PMC article.
• Early demethylation of non-CpG, CpC-rich, elements in the myogenin 5'-flanking region: a priming effect on the spreading of active demethylation.
  Fuso A, Ferraguti G, Grandoni F, Ruggeri R, Scarpa S, Strom R, Lucarelli M. Fuso A, et al. Cell Cycle. 2010 Oct 1;9(19):3965-76. doi: 10.4161/cc.9.19.13193. Epub 2010 Oct 29. Cell Cycle. 2010. PMID: 20935518 Free PMC article.
• Imprinting and epigenetic changes in the early embryo.
  Weaver JR, Susiarjo M, Bartolomei MS. Weaver JR, et al. Mamm Genome. 2009 Sep-Oct;20(9-10):532-43. doi: 10.1007/s00335-009-9225-2. Epub 2009 Sep 16. Mamm Genome. 2009. PMID: 19760320 Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group
  • Ovid Technologies, Inc.

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource
  • REBASE - The Restriction Enzyme Database

• Miscellaneous

  • SciCrunch