Analysis of the 10q23 chromosomal region and the PTEN gene in human sporadic breast carcinoma - PubMed (original) (raw)

Analysis of the 10q23 chromosomal region and the PTEN gene in human sporadic breast carcinoma

H E Feilotter et al. Br J Cancer. 1999 Feb.

Free PMC article

Abstract

We examined a panel of sporadic breast carcinomas for loss of heterozygosity (LOH) in a 10-cM interval on chromosome 10 known to encompass the PTEN gene. We detected allele loss in 27 of 70 breast tumour DNAs. Fifteen of these showed loss limited to a subregion of the area studied. The most commonly deleted region was flanked by D10S215 and D10S541 and encompasses the PTEN locus. We used a combination of denaturing gradient gel electrophoresis and single-strand conformation polymorphism analyses to investigate the presence of PTEN mutations in tumours with LOH in this region. We did not detect mutations of PTEN in any of these tumours. Our data show that, in sporadic breast carcinoma, loss of heterozygosity of the PTEN locus is frequent, but mutation of PTEN is not. These results are consistent with loss of another unidentified tumour suppressor in this region in sporadic breast carcinoma.

PubMed Disclaimer

Similar articles

• Exclusion of a major role for the PTEN tumour-suppressor gene in breast carcinomas.
  Freihoff D, Kempe A, Beste B, Wappenschmidt B, Kreyer E, Hayashi Y, Meindl A, Krebs D, Wiestler OD, von Deimling A, Schmutzler RK. Freihoff D, et al. Br J Cancer. 1999 Feb;79(5-6):754-8. doi: 10.1038/sj.bjc.6690121. Br J Cancer. 1999. PMID: 10070865 Free PMC article.
• Somatic mutation of PTEN in bladder carcinoma.
  Aveyard JS, Skilleter A, Habuchi T, Knowles MA. Aveyard JS, et al. Br J Cancer. 1999 May;80(5-6):904-8. doi: 10.1038/sj.bjc.6690439. Br J Cancer. 1999. PMID: 10360673 Free PMC article.
• Allelic imbalance and mutations of the PTEN gene in ovarian cancer.
  Saito M, Okamoto A, Kohno T, Takakura S, Shinozaki H, Isonishi S, Yasuhara T, Yoshimura T, Ohtake Y, Ochiai K, Yokota J, Tanaka T. Saito M, et al. Int J Cancer. 2000 Jan 15;85(2):160-5. Int J Cancer. 2000. PMID: 10629071
• Common genetic changes between endometriosis and ovarian cancer.
  Obata K, Hoshiai H. Obata K, et al. Gynecol Obstet Invest. 2000;50 Suppl 1:39-43. doi: 10.1159/000052877. Gynecol Obstet Invest. 2000. PMID: 11093060 Review.
• [An approach to early genetic alterations in precancerous cells].
  Sato N, Nishida M, Noguchi M. Sato N, et al. Hum Cell. 2000 Sep;13(3):103-8. Hum Cell. 2000. PMID: 11197771 Review. Japanese.

Cited by

• Targeting the PI3K/AKT/mTOR signaling axis in children with hematologic malignancies.
  Barrett D, Brown VI, Grupp SA, Teachey DT. Barrett D, et al. Paediatr Drugs. 2012 Oct 1;14(5):299-316. doi: 10.2165/11594740-000000000-00000. Paediatr Drugs. 2012. PMID: 22845486 Free PMC article. Review.
• Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast.
  Perren A, Weng LP, Boag AH, Ziebold U, Thakore K, Dahia PL, Komminoth P, Lees JA, Mulligan LM, Mutter GL, Eng C. Perren A, et al. Am J Pathol. 1999 Oct;155(4):1253-60. doi: 10.1016/S0002-9440(10)65227-3. Am J Pathol. 1999. PMID: 10514407 Free PMC article.
• Haploinsufficiency of the Pten tumor suppressor gene promotes prostate cancer progression.
  Kwabi-Addo B, Giri D, Schmidt K, Podsypanina K, Parsons R, Greenberg N, Ittmann M. Kwabi-Addo B, et al. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11563-8. doi: 10.1073/pnas.201167798. Epub 2001 Sep 11. Proc Natl Acad Sci U S A. 2001. PMID: 11553783 Free PMC article.
• The gene for human TATA-binding-protein-associated factor (TAFII) 170: structure, promoter and chromosomal localization.
  Van Der Knaap JA, Van Den Boom V, Kuipers J, Van Eijk MJ, Van Der Vliet PC, Timmers HT. Van Der Knaap JA, et al. Biochem J. 2000 Feb 1;345 Pt 3(Pt 3):521-7. doi: 10.1042/0264-6021:3450521. Biochem J. 2000. PMID: 10642510 Free PMC article.
• Deficiency of Pten accelerates mammary oncogenesis in MMTV-Wnt-1 transgenic mice.
  Li Y, Podsypanina K, Liu X, Crane A, Tan LK, Parsons R, Varmus HE. Li Y, et al. BMC Mol Biol. 2001;2:2. doi: 10.1186/1471-2199-2-2. Epub 2001 Jan 23. BMC Mol Biol. 2001. PMID: 11178110 Free PMC article.

References

1. 1. Cancer Res. 1997 Sep 1;57(17):3657-9 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9052-7 - PubMed
3. 1. Cancer Res. 1997 Sep 15;57(18):3935-40 - PubMed
4. 1. Nat Genet. 1997 Oct;17(2):143-4 - PubMed
5. 1. Cancer Res. 1997 Oct 1;57(19):4183-6 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central

• Medical

  • MedlinePlus Health Information

• Research Materials

  • NCI CPTC Antibody Characterization Program