Dual-colour imaging with GFP variants - PubMed (original) (raw)
Review
Dual-colour imaging with GFP variants
J Ellenberg et al. Trends Cell Biol. 1999 Feb.
Abstract
Green fluorescent protein (GFP) has become an important tool in cell biology and is widely used as a reporter for imaging intracellular proteins and structures in live cells. Recently, spectral variants of GFP with red- and blue-shifted fluorescence emissions have been characterized, opening the possibility of double labelling with two different-coloured GFP fusion proteins. This article reviews recent advances in this technique, with special emphasis on time-lapse imaging applications in living cells.
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