A review of progress towards elucidating the role of protein kinase CK2 in polymerase III transcription: regulation of the TATA binding protein - PubMed (original) (raw)

. 1999 Jan;191(1-2):143-8.

Affiliations

• PMID: 10094403

A review of progress towards elucidating the role of protein kinase CK2 in polymerase III transcription: regulation of the TATA binding protein

A Ghavidel et al. Mol Cell Biochem. 1999 Jan.

Abstract

We have investigated the molecular basis of the requirement for protein kinase CK2 in nuclear transcription in Saccharomyces cerevisiae. In vivo and in vitro analysis has demonstrated that CK2 is required for efficient transcription of the tRNA and 55 rRNA genes by RNA polymerase III. This suggests that a component of the pol III transcription machinery is regulated by CK2. We tested this possibility by a biochemical complementation approach in which components of the pol III transcription machinery from wild type cells were tested for their ability to rescue transcription in extract from a conditionally CK2-deficient mutant. We found that pol III transcription initiation factor IIIB (TFIIIB) fully restores transcription in CK2-deficient extract. Further in vitro studies revealed that TFIIIB must be phosphorylated to be active, that a single subunit of wild type TFIIIB, the TATA binding protein (TBP), is efficiently phosphorylated by CK2, and that recombinant TBP and a limiting amount of CK2 rescues transcription in CK2-deficient extract. We conclude that TBP is the physiological target of CK2 among the components of the pol III transcription machinery. The implications of this result are discussed in the context of previous data concerning the regulation of TFIIIB.

PubMed Disclaimer

Similar articles

• TATA binding protein-associated CK2 transduces DNA damage signals to the RNA polymerase III transcriptional machinery.
  Ghavidel A, Schultz MC. Ghavidel A, et al. Cell. 2001 Sep 7;106(5):575-84. doi: 10.1016/s0092-8674(01)00473-1. Cell. 2001. PMID: 11551505
• Casein kinase II regulation of yeast TFIIIB is mediated by the TATA-binding protein.
  Ghavidel A, Schultz MC. Ghavidel A, et al. Genes Dev. 1997 Nov 1;11(21):2780-9. doi: 10.1101/gad.11.21.2780. Genes Dev. 1997. PMID: 9353248 Free PMC article.
• Cofractionation of the TATA-binding protein with the RNA polymerase III transcription factor TFIIIB.
  Simmen KA, Bernués J, Lewis JD, Mattaj IW. Simmen KA, et al. Nucleic Acids Res. 1992 Nov 25;20(22):5889-98. doi: 10.1093/nar/20.22.5889. Nucleic Acids Res. 1992. PMID: 1461721 Free PMC article.
• Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.
  Huang Y, Maraia RJ. Huang Y, et al. Nucleic Acids Res. 2001 Jul 1;29(13):2675-90. doi: 10.1093/nar/29.13.2675. Nucleic Acids Res. 2001. PMID: 11433012 Free PMC article. Review.
• RNA polymerase III. Genes, factors and transcriptional specificity.
  Willis IM. Willis IM. Eur J Biochem. 1993 Feb 15;212(1):1-11. doi: 10.1111/j.1432-1033.1993.tb17626.x. Eur J Biochem. 1993. PMID: 8444147 Review.

Cited by

• Bioinformatic and in vitro Analyses of Arabidopsis Starch Synthase 2 Reveal Post-translational Regulatory Mechanisms.
  Patterson JA, Tetlow IJ, Emes MJ. Patterson JA, et al. Front Plant Sci. 2018 Sep 19;9:1338. doi: 10.3389/fpls.2018.01338. eCollection 2018. Front Plant Sci. 2018. PMID: 30283470 Free PMC article.
• Coumestrol from the national cancer Institute's natural product library is a novel inhibitor of protein kinase CK2.
  Liu S, Hsieh D, Yang YL, Xu Z, Peto C, Jablons DM, You L. Liu S, et al. BMC Pharmacol Toxicol. 2013 Jul 11;14:36. doi: 10.1186/2050-6511-14-36. BMC Pharmacol Toxicol. 2013. PMID: 23845105 Free PMC article.
• Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription.
  Dumay-Odelot H, Durrieu-Gaillard S, Da Silva D, Roeder RG, Teichmann M. Dumay-Odelot H, et al. Cell Cycle. 2010 Sep 15;9(18):3687-99. doi: 10.4161/cc.9.18.13203. Epub 2010 Sep 1. Cell Cycle. 2010. PMID: 20890107 Free PMC article. Review.
• Chromatin-associated genes protect the yeast genome from Ty1 insertional mutagenesis.
  Nyswaner KM, Checkley MA, Yi M, Stephens RM, Garfinkel DJ. Nyswaner KM, et al. Genetics. 2008 Jan;178(1):197-214. doi: 10.1534/genetics.107.082602. Genetics. 2008. PMID: 18202368 Free PMC article.
• The alpha catalytic subunit of protein kinase CK2 is required for mouse embryonic development.
  Lou DY, Dominguez I, Toselli P, Landesman-Bollag E, O'Brien C, Seldin DC. Lou DY, et al. Mol Cell Biol. 2008 Jan;28(1):131-9. doi: 10.1128/MCB.01119-07. Epub 2007 Oct 22. Mol Cell Biol. 2008. PMID: 17954558 Free PMC article.

References

1. 1. Science. 1994 Jan 7;263(5143):81-4 - PubMed
2. 1. Biochim Biophys Acta. 1990 Sep 24;1054(3):267-84 - PubMed
3. 1. Mol Cell Biol. 1995 Apr;15(4):1983-92 - PubMed
4. 1. Mol Cell Biol. 1997 Apr;17 (4):1868-80 - PubMed
5. 1. Adv Second Messenger Phosphoprotein Res. 1991;23:123-64 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.

• Molecular Biology Databases

  • Saccharomyces Genome Database