Distribution of TNF-alpha, TNF-R55 and TNF-R75 in the rheumatoid synovial membrane: TNF receptors are localized preferentially in the lining layer; TNF-alpha is distributed mainly in the vicinity of TNF receptors in the deeper layers - PubMed (original) (raw)
Distribution of TNF-alpha, TNF-R55 and TNF-R75 in the rheumatoid synovial membrane: TNF receptors are localized preferentially in the lining layer; TNF-alpha is distributed mainly in the vicinity of TNF receptors in the deeper layers
S Alsalameh et al. Scand J Immunol. 1999 Mar.
Free article
Abstract
The expression of TNF-alpha and its receptors in the rheumatoid synovial membrane was investigated using immunohistochemistry and immunocytofluorescence. TNF-alpha+ cells (< 10% of all cells) were found in all regions, predominantly in sublining and diffuse infiltrates. The highest percentage of TNF-R+ cells was found in the lining layer (50-90%), with a slight predominance of TNF-R55. In the sublining, fewer cells expressed TNF-R (approximately 50%), mostly TNF-R75. TNF-R75+ cells were also detectable in diffuse infiltrates and lymphoid aggregates (10-50%). These contained only individual TNF-R55+ cells. In diffuse infiltrates, there were slightly more TNF-R55+ cells than in lymphoid aggregates (in both cases < 10%). In sequential sections, TNF-alpha+ cells localized mostly in the vicinity of TNF-R+ cells. Macrophage-like cells appeared to be the predominant TNF-R+ cell type. CD3+ T cells in lymphoid aggregates expressed exclusively TNF-R75. Subsequently, the expression of membrane-bound TNF-alpha, TNF-R55 and TNF-R75 was tested by FACS analysis in isolated RA synoviocytes (n = 7 patients). Only four specimens expressed mTNF-alpha, and that on a low percentage of cells (2 +/- 2.4%; mean +/- SD). In contrast, all specimens expressed higher percentages of TNF-R55 and TNF-R75 (21 +/- 1% and 14 +/- 7.1%, respectively). These results demonstrate that: (1) the percentage of cells expressing soluble/transmembrane TNF-alpha is greatly outnumbered by the percentage of cells expressing TNF receptors; and (2) TNF-alpha-expressing cells are localized in regions expressing substantial levels of TNF receptors. Therefore, the known pro-inflammatory and pro-arthritic effects of TNF-alpha are probably mediated by local interactions between the receptors and their soluble and transmembrane ligands.
Similar articles
- Tumor necrosis factor alpha and interleukin-1 alpha stimulate late shedding of p75 TNF receptors but not p55 TNF receptors from human monocytes.
Joyce DA, Steer JH. Joyce DA, et al. J Interferon Cytokine Res. 1995 Nov;15(11):947-54. doi: 10.1089/jir.1995.15.947. J Interferon Cytokine Res. 1995. PMID: 8590306 - Osteoarthritic synovial fibroblasts possess an increased level of tumor necrosis factor-receptor 55 (TNF-R55) that mediates biological activation by TNF-alpha.
Alaaeddine N, DiBattista JA, Pelletier JP, Cloutier JM, Kiansa K, Dupuis M, Martel-Pelletier J. Alaaeddine N, et al. J Rheumatol. 1997 Oct;24(10):1985-94. J Rheumatol. 1997. PMID: 9330943 - [TNF].
Yamauchi N, Niitsu Y. Yamauchi N, et al. Gan To Kagaku Ryoho. 1997 Sep;24(11):1486-94. Gan To Kagaku Ryoho. 1997. PMID: 9309146 Review. Japanese. - TNF ligands and receptors--a matter of life and death.
MacEwan DJ. MacEwan DJ. Br J Pharmacol. 2002 Feb;135(4):855-75. doi: 10.1038/sj.bjp.0704549. Br J Pharmacol. 2002. PMID: 11861313 Free PMC article. Review. No abstract available.
Cited by
- TNF in the era of immune checkpoint inhibitors: friend or foe?
Chen AY, Wolchok JD, Bass AR. Chen AY, et al. Nat Rev Rheumatol. 2021 Apr;17(4):213-223. doi: 10.1038/s41584-021-00584-4. Epub 2021 Mar 8. Nat Rev Rheumatol. 2021. PMID: 33686279 Free PMC article. Review. - Phosphorylated Platelet-Derived Growth Factor Receptor-Positive Cells With Anti-apoptotic Properties Accumulate in the Synovium of Patients With Rheumatoid Arthritis.
Matsumura T, Saito Y, Suzuki T, Teramoto A, Ozasa Y, Yamashita T, Fujimiya M, Saito-Chikenji T. Matsumura T, et al. Front Immunol. 2019 Feb 15;10:241. doi: 10.3389/fimmu.2019.00241. eCollection 2019. Front Immunol. 2019. PMID: 30828336 Free PMC article. - Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell.
Dai Y, Ding J, Yin W, He Y, Yu F, Ye C, Hu S, Yu Y. Dai Y, et al. Biomed Res Int. 2018 Oct 25;2018:4941027. doi: 10.1155/2018/4941027. eCollection 2018. Biomed Res Int. 2018. PMID: 30498756 Free PMC article. - B Lymphocytes in Rheumatoid Arthritis and the Effects of Anti-TNF-α Agents on B Lymphocytes: A Review of the Literature.
Pala O, Diaz A, Blomberg BB, Frasca D. Pala O, et al. Clin Ther. 2018 Jun;40(6):1034-1045. doi: 10.1016/j.clinthera.2018.04.016. Epub 2018 May 23. Clin Ther. 2018. PMID: 29801753 Free PMC article. Review. - Immunomodulatory effects of vitamin D in peripheral blood monocyte-derived macrophages from patients with rheumatoid arthritis.
Neve A, Corrado A, Cantatore FP. Neve A, et al. Clin Exp Med. 2014 Aug;14(3):275-83. doi: 10.1007/s10238-013-0249-2. Epub 2013 Jul 4. Clin Exp Med. 2014. PMID: 23824148
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical